The Experts below are selected from a list of 2634 Experts worldwide ranked by ideXlab platform
Tadashi Matsunaga - One of the best experts on this subject based on the ideXlab platform.
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fully automated Chemiluminescence Immunoassay of insulin using antibody protein a bacterial magnetic particle complexes
2000Co-Authors: Tsuyoshi Tanaka, Tadashi MatsunagaAbstract:We report a fully automated sandwich Immunoassay for the determination of human insulin using antibody−protein A−bacterial magnetic particle (BMP) complexes and an alkaline phosphatase-conjugated secondary antibody. BMPs bearing protein A−MagA inserted on the external surface of the membrane were prepared in the Magnetospirillum sp. AMB-1 transconjugant for a protein A−magA fusion gene. MagA protein was used as an anchor to attach protein A onto the membrane. Protein A−BMP complexes harvested from transconjugant AMB-1 were subsequently complexed with anti-human insulin antibodies by specific binding between the Z domain of protein A and the Fc component of IgG to form the antibody−protein A−BMP complexes. The complexes were quite monodisperse after the binding of the antibody. The BMPs' monodispersity resulted in high signal and low noise in the Immunoassay. The luminescence intensity ((kilocounts/s)/μg of antibody) from antibody−protein A−BMP complexes after immunoreaction was higher than that from BMPs ...
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fully automated Chemiluminescence Immunoassay of insulin using antibody protein a bacterial magnetic particle complexes
2000Co-Authors: Tsuyoshi Tanaka, Tadashi MatsunagaAbstract:We report a fully automated sandwich Immunoassay for the determination of human insulin using antibody−protein A−bacterial magnetic particle (BMP) complexes and an alkaline phosphatase-conjugated s...
Linda Julisafrida - One of the best experts on this subject based on the ideXlab platform.
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HUBUNGAN PEMBERIAN ASETILSISTEIN INTRAVENA TERHADAP KADAR PROKALSITONIN PADA PPOK EKSASERBASI DI RUMAH SAKIT UMUM DAERAH DR. ZAINOEL ABIDIN BANDA ACEH HUBUNGAN PEMBERIAN ASETILSISTEIN INTRAVENA TERHADAP KADAR PROKALSITONIN PADA PPOK EKSASERBASI DI RUMAH SAKIT UMUM DAERAH DR. ZAINOEL ABIDIN BANDA ACEH HUBUNGAN PEMBERIAN ASETILSISTEIN INTRAVENA TERHADAP KADAR PROKALSITONIN PADA PPOK EKSASERBASI DI RUMAH SAKIT UMUM DAERAH DR. ZAINOEL ABIDIN BANDA ACEH
2020Co-Authors: Linda JulisafridaAbstract:Latar belakang: Penyakit paru obstruktif kronik (PPOK) merupakan salah satu penyakit tidak menular yang menjadi masalah kesehatan masyarakat di Indonesia dan penyebab kematian keempat di dunia. PPOK eksaserbasi akut masih menjadi masalah kesehatan saat ini yang berdampak terhadap penurunan kualitas hidup, upaya yang dilakukan dalam penanganan pasien PPOK eksaserbasi akut baik terapi farmakologi dan non farmakologi terus berkembang pesat. N-Asetilsistein (NAC) merupakan salah satu terapi farmakologi yang di berikan dengan tujuan tersebut, terapi ini dapat diberikan secara oral maupun intravena. Pemberian NAC dosis tinggi pada pasien PPOK eksaserbasi dapat mengurangi kerusakan sel akibat terjadinya stress okidatif, sehingga dapat mengurangi tingkat eksaserbasi dan memperbaiki kualitas hidup pasien. Metode: Penelitian ini merupakan penelitian analitik dengan desain penelitian cross-sectional. Sampel dikumpulkan dengan metode simple random sampling. Pemeriksaan kadar prokalsitonon dengan metode pemeriksaan Chemiluminescence Immunoassay (CLIA). Hasil: Hasil penelitian didapatkan pasien PPOK eksaserbasi di RSUDZA paling banyak jenis kelamin laki-laki, dengan kelompok umur 50-59 tahun, dengan tingkat pendidikan SLTA, bekerja sebagai wiraswasta, memiliki Indeks Brinkman berat, dengan PPOK eksaserbasi derajat berat, dengan nilai saturasi oksigenBanda Ace
Tsuyoshi Tanaka - One of the best experts on this subject based on the ideXlab platform.
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fully automated Chemiluminescence Immunoassay of insulin using antibody protein a bacterial magnetic particle complexes
2000Co-Authors: Tsuyoshi Tanaka, Tadashi MatsunagaAbstract:We report a fully automated sandwich Immunoassay for the determination of human insulin using antibody−protein A−bacterial magnetic particle (BMP) complexes and an alkaline phosphatase-conjugated secondary antibody. BMPs bearing protein A−MagA inserted on the external surface of the membrane were prepared in the Magnetospirillum sp. AMB-1 transconjugant for a protein A−magA fusion gene. MagA protein was used as an anchor to attach protein A onto the membrane. Protein A−BMP complexes harvested from transconjugant AMB-1 were subsequently complexed with anti-human insulin antibodies by specific binding between the Z domain of protein A and the Fc component of IgG to form the antibody−protein A−BMP complexes. The complexes were quite monodisperse after the binding of the antibody. The BMPs' monodispersity resulted in high signal and low noise in the Immunoassay. The luminescence intensity ((kilocounts/s)/μg of antibody) from antibody−protein A−BMP complexes after immunoreaction was higher than that from BMPs ...
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fully automated Chemiluminescence Immunoassay of insulin using antibody protein a bacterial magnetic particle complexes
2000Co-Authors: Tsuyoshi Tanaka, Tadashi MatsunagaAbstract:We report a fully automated sandwich Immunoassay for the determination of human insulin using antibody−protein A−bacterial magnetic particle (BMP) complexes and an alkaline phosphatase-conjugated s...
Bin Qiao - One of the best experts on this subject based on the ideXlab platform.
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a rolling circle amplified g quadruplex hemin dnazyme for Chemiluminescence Immunoassay of the sars cov 2 protein
2021Co-Authors: Rui Zhang, Bin QiaoAbstract:Sensitive detection of the SARS-CoV-2 protein remains a great research interest in clinical screening and diagnosis owing to the coronavirus epidemic. Here, an ultrasensitive Chemiluminescence (CL) imaging strategy was developed through proximity hybridization to trigger the formation of a rolling circle-amplified G-quadruplex/hemin DNAzyme for the detection of the SARS-CoV-2 protein. The target protein was first recognized by a pair of DNA-antibody conjugates, Ab-1 and Ab-2, to form a proximity-ligated complex, Ab-1/SARS-CoV-2/Ab-2, which contained a DNA sequence complemental to block DNA and thus induced a strand displacement reaction to release the primer from a block/primer complex. The released primer then triggered a rolling circle amplification to form abundant DNAzyme units in the presence of hemin, which produced a strong chemiluminescent signal for the detection of the target protein by catalyzing the oxidation of luminol by hydrogen peroxide. The proposed assay showed a detectable concentration range over 5 orders of magnitude with the detection limit down to 6.46 fg/mL. The excellent selectivity, simple procedure, acceptable accuracy, and intrinsic high throughput of the imaging technique for analysis of serum samples demonstrated the potential applicability of the proposed detection method in clinical screening and diagnosis.
Xiang Fang - One of the best experts on this subject based on the ideXlab platform.
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point of care test system for detection of immunoglobulin g and m against nucleocapsid protein and spike glycoprotein of sars cov 2
2021Co-Authors: Tao Peng, Zhiwei Sui, Zhenghui Huang, Jie Xie, Kai Wen, Yongzhuo Zhang, Wenfeng Huang, Ke Peng, Xinhua Dai, Xiang FangAbstract:Abstract The coronavirus disease 2019 (COVID-19) epidemic continues to ravage the world. In epidemic control, dealing with a large number of samples is a huge challenge. In this study, a point-of-care test (POCT) system was successfully developed and applied for rapid and accurate detection of immunoglobulin-G and -M against nucleocapsid protein (anti-N IgG/IgM) and receptor-binding domain in spike glycoprotein (anti-S-RBD IgG/IgM) of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2). Any one of the IgG/IgM found in a sample was identified as positive. The POCT system contains colloidal gold-based lateral flow Immunoassay test strips, homemade portable reader, and certified reference materials, which detected anti-N and anti-S-RBD IgG/IgM objectively in serum within 15 min. Receiver operating characteristic curve analysis was used to determine the optimal cutoff values, sensitivity, and specificity. It exhibited equal to or better performances than four approved commercial kits. Results of the system and Chemiluminescence Immunoassay kit detecting 108 suspicious samples had high consistency with kappa coefficient at 0.804 (P