The Experts below are selected from a list of 9972 Experts worldwide ranked by ideXlab platform
S D Ehrlich - One of the best experts on this subject based on the ideXlab platform.
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UvrD-dependent replication of Rolling-Circle plasmids in Escherichia coli
Molecular Microbiology, 2000Co-Authors: Claude Bruand, S D EhrlichAbstract:The Escherichia coli UvrD helicase (or helicase II) is known for its involvement in DNA repair. We report that UvrD is required for DNA replication of several different Rolling-Circle plasmids in E. coli, whereas its homologue, the Rep helicase, is not. Lack of UvrD helicase does not impair the first step of plasmid replication, nicking of the double-stranded origin by the plasmid initiator protein. However, replication proceeds no further without UvrD. Indeed, the nicked plasmid molecules accumulate to a high level in uvrD mutants. We conclude that UvrD is the replicative helicase of various Rolling-Circle plasmids. This is the first description of a direct implication of UvrD in DNA replication in vivo.
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Inhibition of a naturally occurring Rolling-Circle replicon in derivatives of the theta-replicating plasmid pIP501
Molecular Microbiology, 1998Co-Authors: Catherine Pujol, Frédéric Chédin, S D Ehrlich, Laurent JannièreAbstract:The mechanisms ensuring regulation of DNA replication in genomes containing multiple replicons are poorly understood. In this report, we addressed this question by analysing in Bacillus subtilis the replication of a derivative of the promiscuous plasmid pIP501 that carries a Rolling-Circle and a theta replicon. Genetic analyses revealed that the Rolling-Circle replicon is strongly inhibited in the derivative and that inhibition requires three elements involved in theta replication: the replication origin, the initiator RepR protein and strong transcription of the repR gene. Inhibition is, however, independent of DNA synthesis at the theta origin. We conclude that Rolling-Circle inhibition is caused by an inhibitory signal encoded by the theta replicon and propose that the signal is composed, at least, of the RepR protein bound to its cognate origin.
Laurent Jannière - One of the best experts on this subject based on the ideXlab platform.
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Inhibition of a naturally occurring Rolling‐Circle replicon in derivatives of the theta‐replicating plasmid pIP501
Molecular Microbiology, 1998Co-Authors: Catherine Pujol, Frédéric Chédin, S. Dusko Ehrlich, Laurent JannièreAbstract:: The mechanisms ensuring regulation of DNA replication in genomes containing multiple replicons are poorly understood. In this report, we addressed this question by analysing in Bacillus subtilis the replication of a derivative of the promiscuous plasmid pIP501 that carries a Rolling-Circle and a theta replicon. Genetic analyses revealed that the Rolling-Circle replicon is strongly inhibited in the derivative and that inhibition requires three elements involved in theta replication: the replication origin, the initiator RepR protein and strong transcription of the repR gene. Inhibition is, however, independent of DNA synthesis at the theta origin. We conclude that Rolling-Circle inhibition is caused by an inhibitory signal encoded by the theta replicon and propose that the signal is composed, at least, of the RepR protein bound to its cognate origin.
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Inhibition of a naturally occurring Rolling-Circle replicon in derivatives of the theta-replicating plasmid pIP501
Molecular Microbiology, 1998Co-Authors: Catherine Pujol, Frédéric Chédin, S D Ehrlich, Laurent JannièreAbstract:The mechanisms ensuring regulation of DNA replication in genomes containing multiple replicons are poorly understood. In this report, we addressed this question by analysing in Bacillus subtilis the replication of a derivative of the promiscuous plasmid pIP501 that carries a Rolling-Circle and a theta replicon. Genetic analyses revealed that the Rolling-Circle replicon is strongly inhibited in the derivative and that inhibition requires three elements involved in theta replication: the replication origin, the initiator RepR protein and strong transcription of the repR gene. Inhibition is, however, independent of DNA synthesis at the theta origin. We conclude that Rolling-Circle inhibition is caused by an inhibitory signal encoded by the theta replicon and propose that the signal is composed, at least, of the RepR protein bound to its cognate origin.
Antoine M. Van Oijen - One of the best experts on this subject based on the ideXlab platform.
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Design of DNA Rolling-Circle templates with controlled fork topology to study mechanisms of DNA replication
Analytical Biochemistry, 2018Co-Authors: Enrico Monachino, Harshad Ghodke, Richard R. Spinks, Ben S. Hoatson, Slobodan Jergic, Zhi-qiang Xu, Nicholas E. Dixon, Antoine M. Van OijenAbstract:Rolling-Circle DNA amplification is a powerful tool employed in biotechnology to produce large from small amounts of DNA. This mode of DNA replication proceeds via a DNA topology that resembles a replication fork, thus also providing experimental access to the molecular mechanisms of DNA replication. However, conventional templates do not allow controlled access to multiple fork topologies, which is an important factor in mechanistic studies. Here we present the design and production of a Rolling-Circle substrate with a tunable length of both the gap and the overhang, and we show its application to the bacterial DNA-replication reaction.
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real time single molecule observation of Rolling Circle dna replication
Nucleic Acids Research, 2009Co-Authors: Nathan A Tanner, Slobodan Jergic, Nicholas E. Dixon, Joseph J Loparo, Samir M Hamdan, Antoine M. Van OijenAbstract:We present a simple technique for visualizing replication of individual DNA molecules in real time. By attaching a Rolling-Circle substrate to a TIRF microscope-mounted flow chamber, we are able to monitor the progression of single-DNA synthesis events and accurately measure rates and processivities of single T7 and Escherichia coli replisomes as they replicate DNA. This method allows for rapid and precise characterization of the kinetics of DNA synthesis and the effects of replication inhibitors.
Catherine Pujol - One of the best experts on this subject based on the ideXlab platform.
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Inhibition of a naturally occurring Rolling‐Circle replicon in derivatives of the theta‐replicating plasmid pIP501
Molecular Microbiology, 1998Co-Authors: Catherine Pujol, Frédéric Chédin, S. Dusko Ehrlich, Laurent JannièreAbstract:: The mechanisms ensuring regulation of DNA replication in genomes containing multiple replicons are poorly understood. In this report, we addressed this question by analysing in Bacillus subtilis the replication of a derivative of the promiscuous plasmid pIP501 that carries a Rolling-Circle and a theta replicon. Genetic analyses revealed that the Rolling-Circle replicon is strongly inhibited in the derivative and that inhibition requires three elements involved in theta replication: the replication origin, the initiator RepR protein and strong transcription of the repR gene. Inhibition is, however, independent of DNA synthesis at the theta origin. We conclude that Rolling-Circle inhibition is caused by an inhibitory signal encoded by the theta replicon and propose that the signal is composed, at least, of the RepR protein bound to its cognate origin.
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Inhibition of a naturally occurring Rolling-Circle replicon in derivatives of the theta-replicating plasmid pIP501
Molecular Microbiology, 1998Co-Authors: Catherine Pujol, Frédéric Chédin, S D Ehrlich, Laurent JannièreAbstract:The mechanisms ensuring regulation of DNA replication in genomes containing multiple replicons are poorly understood. In this report, we addressed this question by analysing in Bacillus subtilis the replication of a derivative of the promiscuous plasmid pIP501 that carries a Rolling-Circle and a theta replicon. Genetic analyses revealed that the Rolling-Circle replicon is strongly inhibited in the derivative and that inhibition requires three elements involved in theta replication: the replication origin, the initiator RepR protein and strong transcription of the repR gene. Inhibition is, however, independent of DNA synthesis at the theta origin. We conclude that Rolling-Circle inhibition is caused by an inhibitory signal encoded by the theta replicon and propose that the signal is composed, at least, of the RepR protein bound to its cognate origin.
Mats Nilsson - One of the best experts on this subject based on the ideXlab platform.
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Formation of Silver Nanostructures by Rolling Circle Amplification Using Boranephosphonate-Modified Nucleotides.
Analytical Chemistry, 2015Co-Authors: Camilla Russell, Saheli Ganguly, Xiaoyan Qian, Marvin H. Caruthers, Mats NilssonAbstract:We investigate the efficiency of incorporation of boranephosphonate-modified nucleotides by phi29 DNA polymerase and present a simple method for forming large defined silver nanostructures by Rolling Circle amplification (RCA) using boranephosphonate internucleotide linkages. RCA is a linear DNA amplification technique that can use specifically circularized DNA probes for detection of target nucleic acids and proteins. The resulting product is a collapsed single-stranded DNA molecule with tandem repeats of the DNA probe. By substituting each of the natural nucleotides with the corresponding 5′-(α-P-borano)deoxynucleosidetriphosphate, only a small reduction in amplification rate is observed. Also, by substituting all four natural nucleotides, it is possible to enzymatically synthesize a micrometer-sized, single-stranded DNA molecule with only boranephosphonate internucleotide linkages. Well-defined silver particles are then readily formed along the Rolling Circle product.
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Compaction of Rolling Circle amplification products increases signal strength and integrity
2014Co-Authors: Carl-magnus Clausson, Ola Söderberg, Linda Arngården, Omer Ishaq, Carolina Wählby, Mats Nilsson, Tomasz KrzywkowskiAbstract:Compaction of Rolling Circle amplification products increases signal strength and integrity
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Padlock probes and Rolling Circle amplification for detection of repeats and single-copy genes in the single-cell comet assay.
Methods of Molecular Biology, 2012Co-Authors: Sara Henriksson, Mats NilssonAbstract:: Padlock probes and Rolling Circle amplification are techniques which can be used for detection of DNA sequences in situ with high specificity and high signal to noise. The single-cell gel electrophoresis assay is used to measure DNA damage and repair in cells. Here, we describe how padlock probes and Rolling Circle amplification can be used to detect DNA sequences within comet preparations.
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real time monitoring of Rolling Circle amplification using a modified molecular beacon design
Nucleic Acids Research, 2002Co-Authors: Mats Nilsson, Mats Gullberg, Fredrik Dahl, Karoly Szuhai, Anton K RaapAbstract:We describe a method to monitor Rolling-Circle replication of circular oligonucleotides in dual-color and in real-time using molecular beacons. The method can be used to study the kinetics of the p ...
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Real‐time monitoring of Rolling‐Circle amplification using a modified molecular beacon design
Nucleic Acids Research, 2002Co-Authors: Mats Nilsson, Mats Gullberg, Fredrik Dahl, Karoly Szuhai, Anton K RaapAbstract:We describe a method to monitor Rolling-Circle replication of circular oligonucleotides in dual-color and in real-time using molecular beacons. The method can be used to study the kinetics of the p ...
