The Experts below are selected from a list of 96 Experts worldwide ranked by ideXlab platform
T A Mcmeekin - One of the best experts on this subject based on the ideXlab platform.
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glaciecola punicea gen nov sp nov and glaciecola pallidula gen nov sp nov psychrophilic bacteria from antarctic sea ice habitats
International Journal of Systematic and Evolutionary Microbiology, 1998Co-Authors: J P Bowman, Sharee A Mccammon, Janelle L Brown, T A McmeekinAbstract:A group of pigmented, psychrophilic, strictly aerobic Chemoheterotrophs isolated from sea-ice cores collected from coastal areas of eastern Antarctica was found to represent a novel 16S rRNA lineage within the gamma subclass of the Proteobacteria, adjacent to the genus Alteromonas. The isolates are motile, Gram-negative, rod-shaped cells, which are psychrophilic and slightly halophilic, and possess an absolute requirement for seawater. Differences in phenotypic characteristics and DNA-DNA hybridization indicated the isolates formed two distinct taxa which have DNA G+C contents of 44–46 mol% and 40 mol%, respectively. Whole-cell fatty acid profiles of the isolates were however very similar and included 16:1ω7c, 18:1ω7c, 16:0 and 17:1ω8c as the major fatty acid components. Overall, sufficient differences exist to distinguish the sea-ice strains from currently recognized bacterial genera. It is proposed the sea-ice strains represent a new genus, Glaciecola, which contains two species, Glaciecola punicea gen. nov., sp. nov. (ACAM 611T) and Glaciecola pallidula gen. nov., sp. nov. (ACAM 615T).
Paul H Blum - One of the best experts on this subject based on the ideXlab platform.
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carbohydrate hydrolysis and transport in the extreme thermoacidophile sulfolobus solfataricus
Applied and Environmental Microbiology, 2012Co-Authors: Sreedevi Lalithambika, Landon Peterson, Karl Dana, Paul H BlumAbstract:Extremely thermoacidophilic microbes, such as Sulfolobus solfataricus, are strict Chemoheterotrophs despite their geologic niche. To clarify their ecophysiology, the overlapping roles of endoglucanases and carbohydrate transporters were examined during growth on soluble cellodextrins as the sole carbon and energy source. Strain-specific differences in genome structure implied a unique role for one of three endogenous endoglucanases. Plasmid-based endoglucanase expression promoted the consumption of oligosaccharides, including cellohexaose (G6) through cellonanaose (G9). Protein transporters required for cellodextrin uptake were identified through mutagenesis and complementation of an ABC transporter cassette, including a putative oligosaccharide binding protein. In addition, ablation of the binding protein compromised growth on glucose and alpha-linked oligosaccharides while inactivation of a previously described glucose transporter had no apparent impact. These data demonstrate that S. solfataricus employs a redundant mechanism for soluble cellodextrin catabolism having both substrate uptake and extracytoplasmic hydrolytic components.
J P Bowman - One of the best experts on this subject based on the ideXlab platform.
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glaciecola punicea gen nov sp nov and glaciecola pallidula gen nov sp nov psychrophilic bacteria from antarctic sea ice habitats
International Journal of Systematic and Evolutionary Microbiology, 1998Co-Authors: J P Bowman, Sharee A Mccammon, Janelle L Brown, T A McmeekinAbstract:A group of pigmented, psychrophilic, strictly aerobic Chemoheterotrophs isolated from sea-ice cores collected from coastal areas of eastern Antarctica was found to represent a novel 16S rRNA lineage within the gamma subclass of the Proteobacteria, adjacent to the genus Alteromonas. The isolates are motile, Gram-negative, rod-shaped cells, which are psychrophilic and slightly halophilic, and possess an absolute requirement for seawater. Differences in phenotypic characteristics and DNA-DNA hybridization indicated the isolates formed two distinct taxa which have DNA G+C contents of 44–46 mol% and 40 mol%, respectively. Whole-cell fatty acid profiles of the isolates were however very similar and included 16:1ω7c, 18:1ω7c, 16:0 and 17:1ω8c as the major fatty acid components. Overall, sufficient differences exist to distinguish the sea-ice strains from currently recognized bacterial genera. It is proposed the sea-ice strains represent a new genus, Glaciecola, which contains two species, Glaciecola punicea gen. nov., sp. nov. (ACAM 611T) and Glaciecola pallidula gen. nov., sp. nov. (ACAM 615T).
Sreedevi Lalithambika - One of the best experts on this subject based on the ideXlab platform.
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carbohydrate hydrolysis and transport in the extreme thermoacidophile sulfolobus solfataricus
Applied and Environmental Microbiology, 2012Co-Authors: Sreedevi Lalithambika, Landon Peterson, Karl Dana, Paul H BlumAbstract:Extremely thermoacidophilic microbes, such as Sulfolobus solfataricus, are strict Chemoheterotrophs despite their geologic niche. To clarify their ecophysiology, the overlapping roles of endoglucanases and carbohydrate transporters were examined during growth on soluble cellodextrins as the sole carbon and energy source. Strain-specific differences in genome structure implied a unique role for one of three endogenous endoglucanases. Plasmid-based endoglucanase expression promoted the consumption of oligosaccharides, including cellohexaose (G6) through cellonanaose (G9). Protein transporters required for cellodextrin uptake were identified through mutagenesis and complementation of an ABC transporter cassette, including a putative oligosaccharide binding protein. In addition, ablation of the binding protein compromised growth on glucose and alpha-linked oligosaccharides while inactivation of a previously described glucose transporter had no apparent impact. These data demonstrate that S. solfataricus employs a redundant mechanism for soluble cellodextrin catabolism having both substrate uptake and extracytoplasmic hydrolytic components.
Brian Austin - One of the best experts on this subject based on the ideXlab platform.
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sneathiella chinensis gen nov sp nov a novel marine alphaproteobacterium isolated from coastal sediment in qingdao china
International Journal of Systematic and Evolutionary Microbiology, 2007Co-Authors: Elizabeth M Jordan, Fabiano L Thompson, Xiaohua Zhang, Marc Vancanneyt, Reiner M Kroppenstedt, Fergus G Priest, Brian AustinAbstract:The taxonomic position of strain LMG 23452(T), which was isolated from coastal sediment from an aquaculture site near Qingdao, China, in 2000, was determined. Strain LMG 23452(T) comprised Gram-negative, non-spore-forming, motile rods and was found to be a halotolerant, aerobic, Chemoheterotroph that produces catalase and oxidase. Comparative 16S rRNA gene sequence analysis revealed that strain LMG 23452(T) shared approximately 89 % sequence similarity with members of the genera Devosia, Hyphomonas, Ensifer and Chelatococcus, which belong to two different orders within the Alphaproteobacteria. Further phylogenetic analysis of the 16S rRNA gene sequence showed that strain LMG 23452(T) formed a separate branch within the order Rhizobiales, falling between the genera Devosia and Ensifer of the families Hyphomicrobiaceae and Rhizobiaceae, respectively. Strain LMG 23452(T) could be differentiated from its closest phylogenetic neighbours on the basis of several phenotypic features, including hydrolysis of the substrates starch and casein and assimilation of the carbohydrates d-glucose, d-mannose, mannitol, maltose and l-arabinose, and chemotaxonomically by the presence of the fatty acids C(14 : 0) 3-OH, C(16 : 1)omega11c, C(16 : 1)omega5c and C(18 : 1)omega5c. The major fatty acids detected in strain LMG 23452(T) were C(18 : 1)omega7c, C(16 : 0), C(19 : 0) cyclo omega8c, C(16 : 1)omega7c and C(17 : 1)omega6c and the G+C content of the genomic DNA was 57.1 mol%. Therefore, the polyphasic data support the placement of strain LMG 23452(T) within a novel genus and species, for which the name Sneathiella chinensis gen. nov., sp. nov. is proposed. The type strain is LMG 23452(T) (=CBMAI 737(T)).