The Experts below are selected from a list of 13704 Experts worldwide ranked by ideXlab platform
Joe G N Garcia - One of the best experts on this subject based on the ideXlab platform.
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sphingosine 1 phosphate released from platelets during clotting accounts for the potent endothelial cell Chemotactic Activity of blood serum and provides a novel link between hemostasis and angiogenesis
The FASEB Journal, 2000Co-Authors: Denis English, Zachary Welch, Thomas A Kovala, Kevin A Harvey, Olga V Volpert, David N Brindley, Joe G N GarciaAbstract:Recent studies have identified factors responsible for angiogenesis within developing tumors, but mediators of vessel formation at sites of trauma, injury, and wound healing are not clearly established. Here we show that sphingosine 1-phosphate (S1P) released by platelets during blood clotting is a potent, specific, and selective endothelial cell chemoattractant that accounts for most of the strong endothelial cell Chemotactic Activity of blood serum, an Activity that is markedly diminished in plasma. Preincubation of endothelial cells with pertussis toxin inhibited this effect of S1P, demonstrating the involvement of a Gαi-coupled receptor. After S1P-induced migration, endothelial cells proliferated avidly and differentiated forming multicellular structures suggestive of early blood vessel formation. S1P was strikingly effective in enhancing the ability of fibroblast growth factor to induce angiogenesis in the avascular mouse cornea. Our results show that blood coagulation initiates endothelial cell angi...
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sphingosine 1 phosphate released from platelets during clotting accounts for the potent endothelial cell Chemotactic Activity of blood serum and provides a novel link between hemostasis and angiogenesis
The FASEB Journal, 2000Co-Authors: Denis English, Zachary Welch, Thomas A Kovala, Kevin A Harvey, Olga V Volpert, David N Brindley, Joe G N GarciaAbstract:Recent studies have identified factors responsible for angiogenesis within developing tumors, but mediators of vessel formation at sites of trauma, injury, and wound healing are not clearly established. Here we show that sphingosine 1-phosphate (S1P) released by platelets during blood clotting is a potent, specific, and selective endothelial cell chemoattractant that accounts for most of the strong endothelial cell Chemotactic Activity of blood serum, an Activity that is markedly diminished in plasma. Preincubation of endothelial cells with pertussis toxin inhibited this effect of S1P, demonstrating the involvement of a Galphai-coupled receptor. After S1P-induced migration, endothelial cells proliferated avidly and differentiated forming multicellular structures suggestive of early blood vessel formation. S1P was strikingly effective in enhancing the ability of fibroblast growth factor to induce angiogenesis in the avascular mouse cornea. Our results show that blood coagulation initiates endothelial cell angiogenic responses through the release of S1P, a potent endothelial cell chemoattractant that exerts its effects by activating a receptor-dependent process.
Mark Gjomarkaj - One of the best experts on this subject based on the ideXlab platform.
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tlr4 upregulation underpins airway neutrophilia in smokers with chronic obstructive pulmonary disease and acute respiratory failure
Human Immunology, 2011Co-Authors: Elisabetta Pace, Maria Ferraro, Liboria Siena, Malcolm Johnson, Antonino Giarratano, Andreina Bruno, Salvatore Mangione, Mark GjomarkajAbstract:Activation of Toll-like receptors (TLR) seems to be involved in the pathogenesis of chronic obstructive pulmonary disease (COPD). Upon TLR activation the release of defensins, including human beta defensin 2 (HBD-2), may occur. In this study, we explored the innate responses in patients with respiratory failure, with and without COPD, requiring intubation and mechanical ventilation. Mini–bronchoalveolar lavage (mini-BAL) samples were collected from nonsmoker subjects without COPD (n = 10), smokers without COPD (n = 6), and smokers with COPD (n = 15). TLR4, TLR2, and HBD-2 expression was evaluated by immunocytochemistry; interleukin (IL)–8, IP-10, and HBD-2 concentrations were evaluated by enzyme-linked immunosorbent assay; Chemotactic Activity toward neutrophils and lymphocytes; and cell apoptosis was evaluated by terminal deoxynucleotidyl transferase dUTP nick end labeling [TUNEL] and by flow cytometry with anti-TLR4 and with HBD-2 depleted and not depleted mini-BAL). COPD mini-BAL showed increased neutrophil numbers, reduced neutrophil apoptosis, increased TLR4 and HBD-2 expression, increased neutrophil Chemotactic Activity, reduced IP-10 concentrations, and reduced lymphocyte Chemotactic Activity compared with those in nonsmoker subjects without COPD. In the smokers without COPD the mini-BAL showed reduced TLR4 and HBD-2 expression, higher IP-10 concentrations, and higher Chemotactic Activity than in patients with COPD. The blocking of TLR4 activation and HBD-2 depletion increased neutrophil apoptosis. No differences were observed for TLR2 expression and IL-8 concentrations. This study strengthens the contribution of TLR4 to promoting airway neutrophilia in COPD.
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cilomilast counteracts the effects of cigarette smoke in airway epithelial cells
Cellular Immunology, 2011Co-Authors: Elisabetta Pace, Maria Ferraro, Malcolm Johnson, Carina Gabriela Uasuf, Antonino Giarratano, Stefania La Grutta, Giuseppe Liotta, Mark GjomarkajAbstract:Abstract Cigarette smoke extracts (CSE) alter TLR4 expression and activation in bronchial epithelial cells. Cilomilast, a phosphodiesterase-4 inhibitor, inhibits cigarette smoke-induced neutrophilia. This study was aimed to explore whether cilomilast, in a human bronchial epithelial cell line (16-HBE), counteracted CSE effects. In particular, TLR4 expression, IP-10 and IL-8 release, lymphocyte and neutrophil Chemotactic Activity and ERK and IkBa phosphorylation in CSE and LPS-stimulated 16-HBE were assessed. CSE increased TLR4 expression, reduced IP-10 release and lymphocyte Chemotactic Activity and increased IL-8 release and neutrophil Chemotactic Activity. Cilomilast reduced TLR4 expression, IL-8 release and neutrophil Chemotactic Activity as well as it increased IP-10 release and lymphocyte Chemotactic Activity. All these cilomilast mediated effects were associated with a reduced ERK1/2 and with an increased IkBa phosphorylation. In conclusion, the present study provides compelling evidences that cilomilast may be considered a possible valid therapeutic option in controlling inflammatory processes present in smokers.
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cigarette smoke increases toll like receptor 4 and modifies lipopolysaccharide mediated responses in airway epithelial cells
Immunology, 2008Co-Authors: Elisabetta Pace, Maria Ferraro, Liboria Siena, Mario Melis, Angela Marina Montalbano, Malcolm Johnson, Maria R Bonsignore, G Bonsignore, Mark GjomarkajAbstract:Airway epithelium is emerging as a regulator of innate immune responses to a variety of insults including cigarette smoke. The main goal of this study was to explore the effects of cigarette smoke extracts (CSE) on Toll-like receptor (TLR) expression and activation in a human bronchial epithelial cell line (16-HBE). The CSE increased the expression of TLR4 and the lipopolysaccharide (LPS) binding, the nuclear factor-kappaB (NF-kappaB) activation, the release of interleukin-8 (IL-8) and the Chemotactic Activity toward neutrophils. It did not induce TLR2 expression or extracellular signal-regulated signal kinase 1/2 (ERK1/2) activation. The LPS increased the expression of TLR4 and induced both NF-kappaB and ERK1/2 activation. The combined exposure of 16-HBE to CSE and LPS was associated with ERK activation rather than NF-kappaB activation and with a further increase of IL-8 release and of Chemotactic Activity toward neutrophils. Furthermore, CSE decreased the constitutive interferon-inducible protein-10 (IP-10) release and counteracted the effect of LPS in inducing both the IP-10 release and the Chemotactic Activity toward lymphocytes. In conclusion, cigarette smoke, by altering the expression and the activation of TLR4 via the preferential release of IL-8, may contribute to the accumulation of neutrophils within the airways of smokers.
Denis English - One of the best experts on this subject based on the ideXlab platform.
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sphingosine 1 phosphate released from platelets during clotting accounts for the potent endothelial cell Chemotactic Activity of blood serum and provides a novel link between hemostasis and angiogenesis
The FASEB Journal, 2000Co-Authors: Denis English, Zachary Welch, Thomas A Kovala, Kevin A Harvey, Olga V Volpert, David N Brindley, Joe G N GarciaAbstract:Recent studies have identified factors responsible for angiogenesis within developing tumors, but mediators of vessel formation at sites of trauma, injury, and wound healing are not clearly established. Here we show that sphingosine 1-phosphate (S1P) released by platelets during blood clotting is a potent, specific, and selective endothelial cell chemoattractant that accounts for most of the strong endothelial cell Chemotactic Activity of blood serum, an Activity that is markedly diminished in plasma. Preincubation of endothelial cells with pertussis toxin inhibited this effect of S1P, demonstrating the involvement of a Gαi-coupled receptor. After S1P-induced migration, endothelial cells proliferated avidly and differentiated forming multicellular structures suggestive of early blood vessel formation. S1P was strikingly effective in enhancing the ability of fibroblast growth factor to induce angiogenesis in the avascular mouse cornea. Our results show that blood coagulation initiates endothelial cell angi...
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sphingosine 1 phosphate released from platelets during clotting accounts for the potent endothelial cell Chemotactic Activity of blood serum and provides a novel link between hemostasis and angiogenesis
The FASEB Journal, 2000Co-Authors: Denis English, Zachary Welch, Thomas A Kovala, Kevin A Harvey, Olga V Volpert, David N Brindley, Joe G N GarciaAbstract:Recent studies have identified factors responsible for angiogenesis within developing tumors, but mediators of vessel formation at sites of trauma, injury, and wound healing are not clearly established. Here we show that sphingosine 1-phosphate (S1P) released by platelets during blood clotting is a potent, specific, and selective endothelial cell chemoattractant that accounts for most of the strong endothelial cell Chemotactic Activity of blood serum, an Activity that is markedly diminished in plasma. Preincubation of endothelial cells with pertussis toxin inhibited this effect of S1P, demonstrating the involvement of a Galphai-coupled receptor. After S1P-induced migration, endothelial cells proliferated avidly and differentiated forming multicellular structures suggestive of early blood vessel formation. S1P was strikingly effective in enhancing the ability of fibroblast growth factor to induce angiogenesis in the avascular mouse cornea. Our results show that blood coagulation initiates endothelial cell angiogenic responses through the release of S1P, a potent endothelial cell chemoattractant that exerts its effects by activating a receptor-dependent process.
David N Brindley - One of the best experts on this subject based on the ideXlab platform.
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sphingosine 1 phosphate released from platelets during clotting accounts for the potent endothelial cell Chemotactic Activity of blood serum and provides a novel link between hemostasis and angiogenesis
The FASEB Journal, 2000Co-Authors: Denis English, Zachary Welch, Thomas A Kovala, Kevin A Harvey, Olga V Volpert, David N Brindley, Joe G N GarciaAbstract:Recent studies have identified factors responsible for angiogenesis within developing tumors, but mediators of vessel formation at sites of trauma, injury, and wound healing are not clearly established. Here we show that sphingosine 1-phosphate (S1P) released by platelets during blood clotting is a potent, specific, and selective endothelial cell chemoattractant that accounts for most of the strong endothelial cell Chemotactic Activity of blood serum, an Activity that is markedly diminished in plasma. Preincubation of endothelial cells with pertussis toxin inhibited this effect of S1P, demonstrating the involvement of a Gαi-coupled receptor. After S1P-induced migration, endothelial cells proliferated avidly and differentiated forming multicellular structures suggestive of early blood vessel formation. S1P was strikingly effective in enhancing the ability of fibroblast growth factor to induce angiogenesis in the avascular mouse cornea. Our results show that blood coagulation initiates endothelial cell angi...
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sphingosine 1 phosphate released from platelets during clotting accounts for the potent endothelial cell Chemotactic Activity of blood serum and provides a novel link between hemostasis and angiogenesis
The FASEB Journal, 2000Co-Authors: Denis English, Zachary Welch, Thomas A Kovala, Kevin A Harvey, Olga V Volpert, David N Brindley, Joe G N GarciaAbstract:Recent studies have identified factors responsible for angiogenesis within developing tumors, but mediators of vessel formation at sites of trauma, injury, and wound healing are not clearly established. Here we show that sphingosine 1-phosphate (S1P) released by platelets during blood clotting is a potent, specific, and selective endothelial cell chemoattractant that accounts for most of the strong endothelial cell Chemotactic Activity of blood serum, an Activity that is markedly diminished in plasma. Preincubation of endothelial cells with pertussis toxin inhibited this effect of S1P, demonstrating the involvement of a Galphai-coupled receptor. After S1P-induced migration, endothelial cells proliferated avidly and differentiated forming multicellular structures suggestive of early blood vessel formation. S1P was strikingly effective in enhancing the ability of fibroblast growth factor to induce angiogenesis in the avascular mouse cornea. Our results show that blood coagulation initiates endothelial cell angiogenic responses through the release of S1P, a potent endothelial cell chemoattractant that exerts its effects by activating a receptor-dependent process.
Thomas A Kovala - One of the best experts on this subject based on the ideXlab platform.
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sphingosine 1 phosphate released from platelets during clotting accounts for the potent endothelial cell Chemotactic Activity of blood serum and provides a novel link between hemostasis and angiogenesis
The FASEB Journal, 2000Co-Authors: Denis English, Zachary Welch, Thomas A Kovala, Kevin A Harvey, Olga V Volpert, David N Brindley, Joe G N GarciaAbstract:Recent studies have identified factors responsible for angiogenesis within developing tumors, but mediators of vessel formation at sites of trauma, injury, and wound healing are not clearly established. Here we show that sphingosine 1-phosphate (S1P) released by platelets during blood clotting is a potent, specific, and selective endothelial cell chemoattractant that accounts for most of the strong endothelial cell Chemotactic Activity of blood serum, an Activity that is markedly diminished in plasma. Preincubation of endothelial cells with pertussis toxin inhibited this effect of S1P, demonstrating the involvement of a Gαi-coupled receptor. After S1P-induced migration, endothelial cells proliferated avidly and differentiated forming multicellular structures suggestive of early blood vessel formation. S1P was strikingly effective in enhancing the ability of fibroblast growth factor to induce angiogenesis in the avascular mouse cornea. Our results show that blood coagulation initiates endothelial cell angi...
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sphingosine 1 phosphate released from platelets during clotting accounts for the potent endothelial cell Chemotactic Activity of blood serum and provides a novel link between hemostasis and angiogenesis
The FASEB Journal, 2000Co-Authors: Denis English, Zachary Welch, Thomas A Kovala, Kevin A Harvey, Olga V Volpert, David N Brindley, Joe G N GarciaAbstract:Recent studies have identified factors responsible for angiogenesis within developing tumors, but mediators of vessel formation at sites of trauma, injury, and wound healing are not clearly established. Here we show that sphingosine 1-phosphate (S1P) released by platelets during blood clotting is a potent, specific, and selective endothelial cell chemoattractant that accounts for most of the strong endothelial cell Chemotactic Activity of blood serum, an Activity that is markedly diminished in plasma. Preincubation of endothelial cells with pertussis toxin inhibited this effect of S1P, demonstrating the involvement of a Galphai-coupled receptor. After S1P-induced migration, endothelial cells proliferated avidly and differentiated forming multicellular structures suggestive of early blood vessel formation. S1P was strikingly effective in enhancing the ability of fibroblast growth factor to induce angiogenesis in the avascular mouse cornea. Our results show that blood coagulation initiates endothelial cell angiogenic responses through the release of S1P, a potent endothelial cell chemoattractant that exerts its effects by activating a receptor-dependent process.