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S.h. Ferreira - One of the best experts on this subject based on the ideXlab platform.
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sephadex induces eosinophil migration to the rat and mouse peritoneal cavity involvement of mast cells ltb4 tnf α il 8 and paf
Inflammation Research, 2002Co-Authors: Sandra Helena Penha De Oliveira, C H S Costa, S.h. Ferreira, Fernando Q. CunhaAbstract:Objective and design: In this study we investigated the Chemotactic mediators involved in the Sephadex-induced eosinophil migration into the peritoneal cavities of rats and mice, and which resident peritoneal cells release these mediators.¶Materials and methods: Sephadex suspension was injected into the peritoneal cavities of rats or mice which were pre-treated, or not, with specific drugs that inhibit synthesis or production of the inflammatory mediators and eosinophil Chemotactic activities were observed. To investigate the role of resident peritoneal cells as a source of these Chemotactic factors, the macrophage population was enhanced or the mast cell population was depleted. The resident cells were also stimulated, in vitro, with Sephadex and the Chemotactic activity of the supernatants was determined.¶Results: Sephadex induced dose and time dependent eosinophil migration in rats and mouse, which were inhibited by dexamethasone and MK 886. BN 52021 only affected the eosinophil migration into the mouse peritoneal cavity. An increase in the macrophage population did not alter the eosinophil migration induced by Sephadex in rat or mouse. However, mast cell population depletion reduced eosinophil migration in rats, but did not alter the migration in mice. Sephadex-stimulated rat mast cells released an eosinophil Chemotactic factor whose release was inhibited by dexamethasone and MK 886. Anti-TNF-α and anti-IL-8 Abs inhibited the Chemotactic activity of the mast cell supernatant.¶Conclusion: Sephadex-induced eosinophil migration into the rat peritoneal cavity is dependent on mast cells, which release LTB4, TNF-α and CINC-1. Conversely, Sephadex-induced eosinophil migration into the mouse peritoneal cavity is mediated by PAF and LTB4, which are not released from resident macrophages or mast cells.¶Abbreviations: LTB4 (leukotriene B4), TNF-α (tumour necrosis factor-α), IL-8 (interleukin-8), PAF (platelet activating factor), IL-1β (interleukin-1β), CINC-1 (cytokine-induced neutrophil chemoattractant-1).
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biological characterization of purified macrophage derived neutrophil Chemotactic factor
Mediators of Inflammation, 1995Co-Authors: Marcelo Diasbaruffi, Fernando Q. Cunha, Maria Cristina Roquebarreira, S.h. FerreiraAbstract:We have recently described the purification of a 54 kDa acidic protein, identified as macrophage-derived neutrophil Chemotactic factor (MNCF). This protein causes in vitro chemotaxis as well as in vivo neutrophil migration even in animals treated with dexamethasone. This in vivo Chemotactic activity of MNCF in animals pretreated with dexamethasone is an uncommon characteristic which discriminates MNCF from known Chemotactic cytokines. MNCF is released in the supernatant by macrophage monolayers stimulated with lipopolysaccharide (LPS). In the present study, we describe some biological characteristics of homogenous purified MNCF. When assayed in vitro, MNCF gave a bell-shaped dose–response curve. This in vitro activity was shown to be caused by haptotaxis. Unlike N-formyl-methionylleucyl- phenylalanine (FMLP) or interleukin 8 (IL-8), the Chemotactic activity of MNCF in vivo and in vitro, was inhibited by preincubation with D-galactose but not with D-mannose. In contrast with IL-8, MNCF did not bind to heparin and antiserum against IL-8 was ineffective in inhibiting its Chemotactic activity. These data indicate that MNCF induces neutrophil migration through a carbohydrate recognition property, but by a mechanism different from that of the known chemokines. It is suggested that MNCF may be an important mediator in the recruitment of neutrophils via the formation of a substrate bound Chemotactic gradient (haptotaxis) in the inflamed tissues.
Fernando Q. Cunha - One of the best experts on this subject based on the ideXlab platform.
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sephadex induces eosinophil migration to the rat and mouse peritoneal cavity involvement of mast cells ltb4 tnf α il 8 and paf
Inflammation Research, 2002Co-Authors: Sandra Helena Penha De Oliveira, C H S Costa, S.h. Ferreira, Fernando Q. CunhaAbstract:Objective and design: In this study we investigated the Chemotactic mediators involved in the Sephadex-induced eosinophil migration into the peritoneal cavities of rats and mice, and which resident peritoneal cells release these mediators.¶Materials and methods: Sephadex suspension was injected into the peritoneal cavities of rats or mice which were pre-treated, or not, with specific drugs that inhibit synthesis or production of the inflammatory mediators and eosinophil Chemotactic activities were observed. To investigate the role of resident peritoneal cells as a source of these Chemotactic factors, the macrophage population was enhanced or the mast cell population was depleted. The resident cells were also stimulated, in vitro, with Sephadex and the Chemotactic activity of the supernatants was determined.¶Results: Sephadex induced dose and time dependent eosinophil migration in rats and mouse, which were inhibited by dexamethasone and MK 886. BN 52021 only affected the eosinophil migration into the mouse peritoneal cavity. An increase in the macrophage population did not alter the eosinophil migration induced by Sephadex in rat or mouse. However, mast cell population depletion reduced eosinophil migration in rats, but did not alter the migration in mice. Sephadex-stimulated rat mast cells released an eosinophil Chemotactic factor whose release was inhibited by dexamethasone and MK 886. Anti-TNF-α and anti-IL-8 Abs inhibited the Chemotactic activity of the mast cell supernatant.¶Conclusion: Sephadex-induced eosinophil migration into the rat peritoneal cavity is dependent on mast cells, which release LTB4, TNF-α and CINC-1. Conversely, Sephadex-induced eosinophil migration into the mouse peritoneal cavity is mediated by PAF and LTB4, which are not released from resident macrophages or mast cells.¶Abbreviations: LTB4 (leukotriene B4), TNF-α (tumour necrosis factor-α), IL-8 (interleukin-8), PAF (platelet activating factor), IL-1β (interleukin-1β), CINC-1 (cytokine-induced neutrophil chemoattractant-1).
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biological characterization of purified macrophage derived neutrophil Chemotactic factor
Mediators of Inflammation, 1995Co-Authors: Marcelo Diasbaruffi, Fernando Q. Cunha, Maria Cristina Roquebarreira, S.h. FerreiraAbstract:We have recently described the purification of a 54 kDa acidic protein, identified as macrophage-derived neutrophil Chemotactic factor (MNCF). This protein causes in vitro chemotaxis as well as in vivo neutrophil migration even in animals treated with dexamethasone. This in vivo Chemotactic activity of MNCF in animals pretreated with dexamethasone is an uncommon characteristic which discriminates MNCF from known Chemotactic cytokines. MNCF is released in the supernatant by macrophage monolayers stimulated with lipopolysaccharide (LPS). In the present study, we describe some biological characteristics of homogenous purified MNCF. When assayed in vitro, MNCF gave a bell-shaped dose–response curve. This in vitro activity was shown to be caused by haptotaxis. Unlike N-formyl-methionylleucyl- phenylalanine (FMLP) or interleukin 8 (IL-8), the Chemotactic activity of MNCF in vivo and in vitro, was inhibited by preincubation with D-galactose but not with D-mannose. In contrast with IL-8, MNCF did not bind to heparin and antiserum against IL-8 was ineffective in inhibiting its Chemotactic activity. These data indicate that MNCF induces neutrophil migration through a carbohydrate recognition property, but by a mechanism different from that of the known chemokines. It is suggested that MNCF may be an important mediator in the recruitment of neutrophils via the formation of a substrate bound Chemotactic gradient (haptotaxis) in the inflamed tissues.
Ghislain Opdenakker - One of the best experts on this subject based on the ideXlab platform.
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in vivo neutrophil recruitment by granulocyte Chemotactic protein 2 is assisted by gelatinase b mmp 9 in the mouse
Journal of Interferon and Cytokine Research, 2000Co-Authors: Anne Dhaese, Anja Wuyts, Chris Dillen, Benedicte Dubois, Alfons Billiau, Hubertine Heremans, Jo Van Damme, Bernd Arnold, Ghislain OpdenakkerAbstract:Granulocyte Chemotactic protein-2 (GCP-2) of the mouse is a potent neutrophil Chemotactic and activating factor in vitro and in vivo. Gelatinase B/matrix metalloproteinase-9 is released from neutro...
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structural and functional identification of two human tumor derived monocyte Chemotactic proteins mcp 2 and mcp 3 belonging to the chemokine family
Journal of Experimental Medicine, 1992Co-Authors: J Van Damme, Paul Proost, J P Lenaerts, Ghislain OpdenakkerAbstract:Cytokine-stimulated human osteosarcoma cells (MG-63) secrete several related Chemotactic factors, including the neutrophil-activating protein interleukin 8 (IL-8) and the monocyte Chemotactic protein (MCP)-1. We describe the isolation and characterization of two novel monocyte Chemotactic factors from this tumor cell line. Although these proteins copurified with MCP-1 and IL-8 on heparin-Sepharose, they could be separated by cation-exchange fast protein liquid chromatography and reverse-phase high-performance liquid chromatography. The corresponding 7.5- and 11-kD proteins were NH2-terminally blocked but were identified by sequencing peptide fragments. They showed a primary structure mostly related to that of MCP-1 and were therefore designated MCP-2 and MCP-3, respectively. These molecules can be classified in a subfamily of proinflammatory proteins characterized by the conservation of cysteine residues. MCP-2 and MCP-3 are also functionally related to MCP-1 because they specifically attract monocytes, but not neutrophils, in vitro. The Chemotactic potency (specific activity) was comparable for all three MCPs. Intradermal injection of these proteins in rabbits resulted in selective monocyte recruitment in vivo. Since tumor cells are good producers of leukocyte Chemotactic factors, it could be questioned whether these molecules can indirectly control tumor growth by attracting leukocytes or whether they rather promote invasion by the secretion of proteases from the attracted cells.
Hidenobu Tanihara - One of the best experts on this subject based on the ideXlab platform.
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monocyte Chemotactic protein 1 level in the aqueous humour as a prognostic factor for the outcome of trabeculectomy
Clinical and Experimental Ophthalmology, 2014Co-Authors: Toshihiro Inoue, Takahiro Kawaji, Hidenobu TaniharaAbstract:Background The aim of this study was to elucidate the effects of the aqueous humour monocyte Chemotactic protein-1 level on the surgical outcome of trabeculectomy in cases of phakic glaucoma. Design Retrospective study. Participants Fifty-three cases of open-angle glaucoma. Methods At the time of trabeculectomy, aqueous humour samples were collected before any incisions were made. The monocyte Chemotactic protein-1 level was then determined by immunoassay. The Cox proportional hazards test was used to analyse the clinical factors related to failure of trabeculectomy. Subsequently, the success probability was calculated by Kaplan–Meier analysis and cumulative success probabilities were analysed by the log-rank test. Main Outcome Measures Probability of success. Results In 30 phakic glaucoma and 23 pseudophakic glaucoma cases, the mean monocyte Chemotactic protein-1 levels in aqueous humour were 1165.2 and 2152.9 pg/mL, respectively; the difference was statistically significant (P < 0.0001). Univariate analysis showed that the outcomes of surgery were correlated with the monocyte Chemotactic protein-1 level, but not with age, gender, diagnosis of exfoliation glaucoma, pseudophakic status or pretrabeculectomy intraocular pressure. Subsequent multivariate analysis revealed that only the aqueous humour monocyte Chemotactic protein-1 level was significantly correlated with the outcomes of trabeculectomy (P = 0.043). Among the 30 phakic glaucoma eyes, the success probabilities after trabeculectomy were significantly different between the high and low monocyte Chemotactic protein-1 groups (P = 0.018). Conclusion In open-angle glaucoma patients, the monocyte Chemotactic protein-1 level is a prognostic factor for the results of trabeculectomy.
Rodolphe Maheux - One of the best experts on this subject based on the ideXlab platform.
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increased monocyte Chemotactic protein 1 level and activity in the peripheral blood of women with endometriosis
American Journal of Obstetrics and Gynecology, 1996Co-Authors: Ali Akoum, Andre Lemay, Shaun R Mccoll, Isabelle Paradis, Rodolphe MaheuxAbstract:Abstract OBJECTIVE: Our purpose was to evaluate monocyte Chemotactic protein-1 in the peripheral blood of women with and without endometriosis. STUDY DESIGN: Fifty-seven patients with endometriosis at laparoscopy done for infertility and pelvic pain were compared with 44 fertile women with no evidence of endometriosis at tubal ligation by laparoscopy. Monocyte Chemotactic protein-1 concentration in the plasma was determined by enzyme-linked immunosorbent assay and its biologic activity was evaluated by measuring monocyte chemotaxis with use of a human histiocytic cell line (U937). RESULTS: Monocyte Chemotactic protein-1 concentrations (median and range of values) found in the plasma were higher in patients with endometriosis (163, 0 to 788 pg/ml) than in normal controls (0, 0 to 355 pg/ml). This elevation was significant only in the minimal stage of endometriosis (revised American Fertility Society stage I). However, increased Chemotactic activity (mean number of migrating cells/mm 2 ± SEM) was found in the stages I (1240 ± 141), II (519 ± 30), and III-IV (523 ± 23) of the disease compared with normal controls (205 ± 20). A total of 35% to 44% of this activity was inhibited in the presence of an antibody specific to monocyte Chemotactic protein-1. CONCLUSION: Endometriosis is associated with increased level and activity of monocyte Chemotactic protein-1 in the peripheral blood. The elevation and activation of this cytokine could play a relevant role in the immunoinflammatory process associated with the disease. (Am J Obstet Gynecol 1996;175:1620-5.)