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Xiaodong Zheng - One of the best experts on this subject based on the ideXlab platform.
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effect of cryptococcus laurentii on inducing disease resistance in Cherry Tomato fruit with focus on the expression of defense related genes
Food Chemistry, 2018Co-Authors: Ting Yu, Xiaodong Zheng, Qiang Wang, Yanwen Zhang, Huangping LuAbstract:Abstract The objective of this study was to prove and explain the disease resistance-inducing ability of Cryptococcus laurentii on Cherry Tomato, as well as assay its effect on fruit quality. Apart from disease incidence, activities of defense-related enzymes and expression of critical genes were studied. With pre-treatment of C. laurentii, disease incidences of Botrytis cinerea and Alternaria alternate infected fruits were both significantly reduced. Corresponding mechanism could be explained as C. laurentii can induce resistance in Cherry Tomato by activating the expression of important defense-related genes, such as genes involved in salicylic acid (SA) and jasmonic acid (JA) signaling pathways and genes encoding pathogenesis related proteins, thus activating comprehensive defense reaction against pathogen invasion. Coupled with the results that fruit color was improved and other physicochemical parameters remained uninfluenced, our study suggests that pre-treatment with C. laurentii can be a promising method to preserve Cherry Tomato fruits.
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Biocontrol activity of a cold-adapted yeast from Tibet against gray mold in Cherry Tomato and its action mechanism
Extremophiles, 2017Co-Authors: Hao Hu, Michael E. Wisniewski, Ahmed Abdelfattah, Xiaodong ZhengAbstract:Cold-adapted biocontrol yeast was selected from four yeast isolates from Tibet against gray mold of Cherry Tomato in cold storage. The strain numbered LB2 showed the best biocontrol activity and identified as Cryptococcus laurentii. Competition for nutrient, space, and induced fruit resistance was also its antagonistic mechanism. Compared with C. laurentii from sea-level place, the reason why LB2 had a better biocontrol activity was studied. More trehalose and proline in cell of LB2 made it exhibit a better cellular activity at low temperature, such as higher population dynamics in the wounds of Cherry Tomato and more biocontrol-related enzyme secretion, chitinase and β-glucanase. The better oxidative stress tolerance was another characteristic of LB2. Maybe because of the ideal culture condition, there was no obvious difference between these two yeasts in the growth in vitro test at low temperature. Although the same phenomenon existed in the low pH stress test, LB2 still had higher cell concentration under this stress. Comparative transcriptomics method was also applied to analyze the cell activity of LB2 and C. laurentii at different temperatures. The results showed that more active response in the intracellular structure and intracellular metabolic process to cold temperature made LB2 had a better activity. The present study indicated a possibility to select cold-adapted biocontrol yeast from Tibet and also showed its primary action mechanism.
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Rhamnolipids induce oxidative stress responses in Cherry Tomato fruit to Alternaria alternata.
Pest Management Science, 2015Co-Authors: Hao Hu, Laifeng Lu, Xiaodong ZhengAbstract:BACKGROUND Rhamnolipids showed an antimicrobial activity applicable to a variety of pathogenic microorganisms, but mechanisms were mostly focused on their direct inhibitory effect. RESULTS This study showed that disease incidence obviously decreased when Cherry Tomatoes were treated with rhamnolipids, whether before or after Alternaria alternata inoculation. The activities of antioxidant enzymes, including superoxide dismutase and catalase, were increased in rhamnolipid-pretreated Cherry Tomato inoculated with A. alternata within 12 h, while contents of reactive oxygen species decreased. Moreover, resistant response of Cherry Tomato treated with rhamnolipids and A. alternata was also attributed to activity stimulation of ascorbate peroxidase and glutathione reductase, accompanied with an increase in reduced glutathione, which is beneficial for scavenging excessive H2O2. CONCLUSION Results indicated that rhamnolipids could effectively reduce fungal disease of harvested Cherry Tomato by inducing fruit resistance and mechanisms involved in elicitation of antioxidative reactions such as the ability to scavenge excess reactive oxygen species. © 2015 Society of Chemical Industry
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effect of rhamnolipids on rhodotorula glutinis biocontrol of alternaria alternata infection in Cherry Tomato fruit
Postharvest Biology and Technology, 2014Co-Authors: Shixiang Xu, Yushu Chen, Xiaodong ZhengAbstract:Abstract This study was conducted to evaluate the efficacy of the biocontrol yeast, Rhodotorula glutinis with a surfactant produced by Pseudomonas aeruginosa , rhamnolipids, on the inhibition of Alternaria alternata on Cherry Tomato fruit. A combination of R. glutinis with rhamnolipids at 500 μg/mL was more effective in suppressing A. alternata infection than application of R. glutinis or rhamnolipids alone. Moreover, integration of R. glutinis with rhamnolipids significantly stimulated peroxidase, polyphenoloxidase and phenylalanine ammonialyase activities of Cherry Tomato fruit, which were stronger than the single treatments. Rhamnolipids with reduced concentrations did not affect the growth of R. glutinis in vitro , but were utilized as carbon sources by yeasts under conditions of limited carbon sources. Moreover, rhamnolipids themselves reduced decay incidence of A. alternata on Cherry Tomato fruit and promoted the population growth of R. glutinis in vivo . This combined treatment could induce natural resistance as well as accelerate colonization of yeasts on the fruit surface, providing an effective and safe strategy to control postharvest diseases.
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effect of citronella essential oil on the inhibition of postharvest alternaria alternata in Cherry Tomato
Journal of the Science of Food and Agriculture, 2014Co-Authors: Qianru Chen, Xiaodong Zheng, Shixiang Xu, Tao Wu, Ting YuAbstract:BACKGROUND: Essential oils such as citronella oil exhibit antifungal activity and are potential alternative inhibitors to chemical synthetic fungicides for controlling postharvest diseases. In this study the antifungal activity of citronella oil against Alternaria alternata was investigated. RESULTS: In vitro, citronella oil showed strong inhibition activity against A. alternata. The minimum inhibitory concentration in potato dextrose agar and potato dextrose broth medium was determined as 1 and 0.8 µL mL(-1) respectively. In vivo the disease incidence of Lycopersicon esculentum (Cherry Tomato) treated with citronella oil was significantly (P < 0.05) reduced compared with the control after 5 days of storage at 25 °C and 95% relative humidity. The disease incidence at oil concentrations of 0.2-1.5 µL mL(-1) was 88-48%. The most effective dosage of the oil was 1.5 µL mL(-1), with 52% reduction, and the oil had no negative effect on fruit quality. Scanning electron microscopy observation revealed considerably abnormal mycelial morphology. CONCLUSION: Citronella oil can significantly inhibit A. alternata in vitro and in vivo and has potential as a promising natural product for controlling black rot in Cherry Tomato.
Jishuang Chen - One of the best experts on this subject based on the ideXlab platform.
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microarray analysis of gene expression profile induced by the biocontrol yeast cryptococcus laurentii in Cherry Tomato fruit
Gene, 2009Co-Authors: Feng Jiang, Xiaodong Zheng, Jishuang ChenAbstract:Abstract To obtain an overall view on transcript modification during the Cherry Tomato fruit responding to biocontrol yeast Cryptococcus laurentii , we performed a microarrary analysis, using Affymetrix Tomato Genechip arrays, representing approximately 10,000 genes. The results showed that 194 and 312 genes were up- or down-regulated, respectively, more then ten time fold (log2 change ratio ≥ 2 or log2 change ratio ≤ − 2) in biocontrol yeast treated Tomato fruit as compared with control fruits. Those up-regulated included genes involved in metabolism, signal transduction, and stress response. Conversely, genes related to energy metabolism and photosynthesis were generally down-regulated. Our results suggest that biocontrol yeast treatment induces fruit resistance response, suppresses energy metabolism and photosynthesis. A number of transcripts encoding proteins/enzymes which are known to be up-regulated under some biotic and abiotic stress are also up-regulated after the applying biological control yeast to Cherry Tomato fruit. The expression of these proteins might increase the fruit resistance towards postharvest pathogen infection and damage.
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Microarray analysis of gene expression profile induced by the biocontrol yeast Cryptococcus laurentii in Cherry Tomato fruit
Gene, 2009Co-Authors: Feng Jiang, Xiaodong Zheng, Jishuang ChenAbstract:To obtain an overall view on transcript modification during the Cherry Tomato fruit responding to biocontrol yeast Cryptococcus laurentii, we performed a microarrary analysis, using Affymetrix Tomato Genechip arrays, representing approximately 10,000 genes. The results showed that 194 and 312 genes were up- or down-regulated, respectively, more then ten time fold (log2 change ratio ??? 2 or log2 change ratio ??? - 2) in biocontrol yeast treated Tomato fruit as compared with control fruits. Those up-regulated included genes involved in metabolism, signal transduction, and stress response. Conversely, genes related to energy metabolism and photosynthesis were generally down-regulated. Our results suggest that biocontrol yeast treatment induces fruit resistance response, suppresses energy metabolism and photosynthesis. A number of transcripts encoding proteins/enzymes which are known to be up-regulated under some biotic and abiotic stress are also up-regulated after the applying biological control yeast to Cherry Tomato fruit. The expression of these proteins might increase the fruit resistance towards postharvest pathogen infection and damage. ?? 2008 Elsevier B.V. All rights reserved.
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Identification of differentially expressed genes from Cherry Tomato fruit (Lycopersicon esculentum) after application of the biological control yeast Cryptococcus laurentii
Postharvest Biology and Technology, 2009Co-Authors: Feng Jiang, Karin Krupinska, Ying Miao, Jishuang Chen, Xiaodong ZhengAbstract:Postharvest decay of fruit may be controlled by the use of a variety of diverse microorganisms acting as biocontrol agents, but the mechanisms associated with control are not fully understood. In order to gain insight into the action of antagonistic microorganisms on fruit, a forward subtractive suppression hybridization (SSH) cDNA library was constructed. SSH was performed with cDNA from Cherry Tomato fruit (Lycopersicon esculentum) inoculated with water as the "driver" and cDNA from Tomato fruit inoculated by Cryptococcus laurentii as the "tester". A total of 150 clones in the SSH library were sequenced and found to represent 50 unigenes. BLASTX results reveal that 35 cDNAs had significant sequence homologies with known sequences in the NCBI database. The identified cDNAs encode proteins involved in cellular processes such as primary metabolism, signal transduction, defense and responses to pathogens, stress-related, cell wall assembly, and photosynthesis and transcription related sequences. Six cDNA clones were selected for temporal expression analysis using RT-PCR. The results show that a number of transcripts encoding proteins/enzymes which are known to be up-regulated under some biotic and abiotic stresses are also up-regulated after the application of biological control yeast to Cherry Tomato fruit. The expression of these proteins may play a role in increasing fruit resistance to postharvest pathogen infection. ?? 2009 Elsevier B.V. All rights reserved.
Feng Jiang - One of the best experts on this subject based on the ideXlab platform.
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microarray analysis of gene expression profile induced by the biocontrol yeast cryptococcus laurentii in Cherry Tomato fruit
Gene, 2009Co-Authors: Feng Jiang, Xiaodong Zheng, Jishuang ChenAbstract:Abstract To obtain an overall view on transcript modification during the Cherry Tomato fruit responding to biocontrol yeast Cryptococcus laurentii , we performed a microarrary analysis, using Affymetrix Tomato Genechip arrays, representing approximately 10,000 genes. The results showed that 194 and 312 genes were up- or down-regulated, respectively, more then ten time fold (log2 change ratio ≥ 2 or log2 change ratio ≤ − 2) in biocontrol yeast treated Tomato fruit as compared with control fruits. Those up-regulated included genes involved in metabolism, signal transduction, and stress response. Conversely, genes related to energy metabolism and photosynthesis were generally down-regulated. Our results suggest that biocontrol yeast treatment induces fruit resistance response, suppresses energy metabolism and photosynthesis. A number of transcripts encoding proteins/enzymes which are known to be up-regulated under some biotic and abiotic stress are also up-regulated after the applying biological control yeast to Cherry Tomato fruit. The expression of these proteins might increase the fruit resistance towards postharvest pathogen infection and damage.
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Microarray analysis of gene expression profile induced by the biocontrol yeast Cryptococcus laurentii in Cherry Tomato fruit
Gene, 2009Co-Authors: Feng Jiang, Xiaodong Zheng, Jishuang ChenAbstract:To obtain an overall view on transcript modification during the Cherry Tomato fruit responding to biocontrol yeast Cryptococcus laurentii, we performed a microarrary analysis, using Affymetrix Tomato Genechip arrays, representing approximately 10,000 genes. The results showed that 194 and 312 genes were up- or down-regulated, respectively, more then ten time fold (log2 change ratio ??? 2 or log2 change ratio ??? - 2) in biocontrol yeast treated Tomato fruit as compared with control fruits. Those up-regulated included genes involved in metabolism, signal transduction, and stress response. Conversely, genes related to energy metabolism and photosynthesis were generally down-regulated. Our results suggest that biocontrol yeast treatment induces fruit resistance response, suppresses energy metabolism and photosynthesis. A number of transcripts encoding proteins/enzymes which are known to be up-regulated under some biotic and abiotic stress are also up-regulated after the applying biological control yeast to Cherry Tomato fruit. The expression of these proteins might increase the fruit resistance towards postharvest pathogen infection and damage. ?? 2008 Elsevier B.V. All rights reserved.
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Identification of differentially expressed genes from Cherry Tomato fruit (Lycopersicon esculentum) after application of the biological control yeast Cryptococcus laurentii
Postharvest Biology and Technology, 2009Co-Authors: Feng Jiang, Karin Krupinska, Ying Miao, Jishuang Chen, Xiaodong ZhengAbstract:Postharvest decay of fruit may be controlled by the use of a variety of diverse microorganisms acting as biocontrol agents, but the mechanisms associated with control are not fully understood. In order to gain insight into the action of antagonistic microorganisms on fruit, a forward subtractive suppression hybridization (SSH) cDNA library was constructed. SSH was performed with cDNA from Cherry Tomato fruit (Lycopersicon esculentum) inoculated with water as the "driver" and cDNA from Tomato fruit inoculated by Cryptococcus laurentii as the "tester". A total of 150 clones in the SSH library were sequenced and found to represent 50 unigenes. BLASTX results reveal that 35 cDNAs had significant sequence homologies with known sequences in the NCBI database. The identified cDNAs encode proteins involved in cellular processes such as primary metabolism, signal transduction, defense and responses to pathogens, stress-related, cell wall assembly, and photosynthesis and transcription related sequences. Six cDNA clones were selected for temporal expression analysis using RT-PCR. The results show that a number of transcripts encoding proteins/enzymes which are known to be up-regulated under some biotic and abiotic stresses are also up-regulated after the application of biological control yeast to Cherry Tomato fruit. The expression of these proteins may play a role in increasing fruit resistance to postharvest pathogen infection. ?? 2009 Elsevier B.V. All rights reserved.
Kang Tu - One of the best experts on this subject based on the ideXlab platform.
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defense response of Cherry Tomato at different maturity stages to combined treatment of hot air and cryptococcus laurentii
Postharvest Biology and Technology, 2016Co-Authors: Miao Xu, Sicong Tu, Hailun Wu, Kang TuAbstract:Abstract Hot air (HA) treatment (38 °C, 12 h) and Cryptococcus laurentii were investigated separately or in combination for controlling postharvest decay caused by artificially inoculated with Botrytis cinerea , or natural infection in Cherry Tomato at different maturity stages. Results indicated that the combined treatment significantly reduced disease incidence of gray mold in Cherry Tomato at both mature green and pink stages regardless fruit were artificially inoculated with B. cinerea before or after treatment. The disease incidence of mature green fruit was lower than that of pink fruit with the same treatment. Similar results were observed on testing fruit with natural infection. HA could postpone fruit softening and increase the population density of C. laurentii in fruit wounds. Moreover, HA combined with C. laurentii induced higher activities of peroxidase, phenylalanine ammonia-lyase, chitinase, and β-1, 3-glucanases than the control. These results suggest that HA combined with C. laurentii had a curative and preventive effect to control postharvest disease in Cherry Tomato at both mature green and pink stages.
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a combination of heat treatment and pichia guilliermondii prevents Cherry Tomato spoilage by fungi
International Journal of Food Microbiology, 2010Co-Authors: Yan Zhao, Kang Tu, Sicong Tu, Jing SuAbstract:Abstract This study investigated the effectiveness of heat treatment and Pichia guilliermondii , either alone or in combination, to combat postharvest fungal spoilage in Cherry Tomato fruit. In vitro experiments demonstrated that heat treatment at 38 °C significantly inhibited mycelial growth of three different pathogens ( Botrytis cinerea , Alternaria alternata and Rhizopus stolonifer Ehrenb). In vivo experiments unveiled that either heat treatment or P. guilliermondii reduced decay caused by these pathogens. Furthermore, a combination of heat treatment followed by the application of P. guilliermondii (H + P) provided the best efficacy in prevention of Cherry Tomato from fungal spoilage. Following, H + P treatment, electronic nose detected a reduction of volatility in Cherry Tomato fruit odor, an indicator of preserving fruit's freshness. Scanning electron microscopy unveiled that heat treatment at 38 °C for 24 h inhibited hyphae growth and spore germination of R. stolonifer Ehrenb while P. guilliermondii multiplied rapidly on fruit wounds, and its cells had a strong capability of adhesion to the hyphae of R. stolonifer Ehrenb. However, heat treatment also seriously injured P. guilliermondii , therefore P. guilliermondii should be applied after heat treatment. A combination of heat treatment and P. guilliermondii is one of the most effective techniques at controlling postharvest fungal spoilage in Cherry Tomato fruit.
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heat treatment in combination with antagonistic yeast reduces diseases and elicits the active defense responses in harvested Cherry Tomato fruit
Journal of Agricultural and Food Chemistry, 2009Co-Authors: Yan Zhao, Kang Tu, Jing Su, Sicong TuAbstract:This study investigated the effects of heat treatment (hot air at 38 °C) and antagonistic yeast (Pichia guilliermondii) alone or in combination against postharvest diseases (Botrytis cinerea, Alternaria alternata and Rhizopus nigricans) on Cherry Tomato fruit, and evaluated the elicitation of active defense responses. Results showed that heat treatment at 38 °C for 24 h in combination with P. guilliermondii at 1 × 108 CFU mL−1 was the most effective approach to reduce various infections on Cherry Tomato fruit’s wounds. Moreover, the combined heat and P. guilliermondii treatment stimulated a rapid increase of H2O2 and higher lignin deposition in Cherry Tomato fruit showing that the oxidative burst and biological synthesis of lignin might play important roles in the fruit’s active defense responses. In addition, the reduction of the fruit’s susceptibility to pathogens by the combined treatment was positively correlated with higher activities of phenylalanine ammonia-lyase (PAL) and β-1,3-glucanase in Cherry...
Sicong Tu - One of the best experts on this subject based on the ideXlab platform.
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defense response of Cherry Tomato at different maturity stages to combined treatment of hot air and cryptococcus laurentii
Postharvest Biology and Technology, 2016Co-Authors: Miao Xu, Sicong Tu, Hailun Wu, Kang TuAbstract:Abstract Hot air (HA) treatment (38 °C, 12 h) and Cryptococcus laurentii were investigated separately or in combination for controlling postharvest decay caused by artificially inoculated with Botrytis cinerea , or natural infection in Cherry Tomato at different maturity stages. Results indicated that the combined treatment significantly reduced disease incidence of gray mold in Cherry Tomato at both mature green and pink stages regardless fruit were artificially inoculated with B. cinerea before or after treatment. The disease incidence of mature green fruit was lower than that of pink fruit with the same treatment. Similar results were observed on testing fruit with natural infection. HA could postpone fruit softening and increase the population density of C. laurentii in fruit wounds. Moreover, HA combined with C. laurentii induced higher activities of peroxidase, phenylalanine ammonia-lyase, chitinase, and β-1, 3-glucanases than the control. These results suggest that HA combined with C. laurentii had a curative and preventive effect to control postharvest disease in Cherry Tomato at both mature green and pink stages.
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a combination of heat treatment and pichia guilliermondii prevents Cherry Tomato spoilage by fungi
International Journal of Food Microbiology, 2010Co-Authors: Yan Zhao, Kang Tu, Sicong Tu, Jing SuAbstract:Abstract This study investigated the effectiveness of heat treatment and Pichia guilliermondii , either alone or in combination, to combat postharvest fungal spoilage in Cherry Tomato fruit. In vitro experiments demonstrated that heat treatment at 38 °C significantly inhibited mycelial growth of three different pathogens ( Botrytis cinerea , Alternaria alternata and Rhizopus stolonifer Ehrenb). In vivo experiments unveiled that either heat treatment or P. guilliermondii reduced decay caused by these pathogens. Furthermore, a combination of heat treatment followed by the application of P. guilliermondii (H + P) provided the best efficacy in prevention of Cherry Tomato from fungal spoilage. Following, H + P treatment, electronic nose detected a reduction of volatility in Cherry Tomato fruit odor, an indicator of preserving fruit's freshness. Scanning electron microscopy unveiled that heat treatment at 38 °C for 24 h inhibited hyphae growth and spore germination of R. stolonifer Ehrenb while P. guilliermondii multiplied rapidly on fruit wounds, and its cells had a strong capability of adhesion to the hyphae of R. stolonifer Ehrenb. However, heat treatment also seriously injured P. guilliermondii , therefore P. guilliermondii should be applied after heat treatment. A combination of heat treatment and P. guilliermondii is one of the most effective techniques at controlling postharvest fungal spoilage in Cherry Tomato fruit.
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heat treatment in combination with antagonistic yeast reduces diseases and elicits the active defense responses in harvested Cherry Tomato fruit
Journal of Agricultural and Food Chemistry, 2009Co-Authors: Yan Zhao, Kang Tu, Jing Su, Sicong TuAbstract:This study investigated the effects of heat treatment (hot air at 38 °C) and antagonistic yeast (Pichia guilliermondii) alone or in combination against postharvest diseases (Botrytis cinerea, Alternaria alternata and Rhizopus nigricans) on Cherry Tomato fruit, and evaluated the elicitation of active defense responses. Results showed that heat treatment at 38 °C for 24 h in combination with P. guilliermondii at 1 × 108 CFU mL−1 was the most effective approach to reduce various infections on Cherry Tomato fruit’s wounds. Moreover, the combined heat and P. guilliermondii treatment stimulated a rapid increase of H2O2 and higher lignin deposition in Cherry Tomato fruit showing that the oxidative burst and biological synthesis of lignin might play important roles in the fruit’s active defense responses. In addition, the reduction of the fruit’s susceptibility to pathogens by the combined treatment was positively correlated with higher activities of phenylalanine ammonia-lyase (PAL) and β-1,3-glucanase in Cherry...