The Experts below are selected from a list of 25653 Experts worldwide ranked by ideXlab platform
S Natkunam - One of the best experts on this subject based on the ideXlab platform.
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combined detection and genotyping of Chikungunya virus by a specific reverse transcription polymerase chain reaction
Journal of Medical Virology, 2002Co-Authors: Futoshi Hasebe, Maria Del Carmen Parquet, Basu Dev Pandey, Edward Gitau Matumbi Mathenge, Kouichi Morita, V Balasubramaniam, Z Saat, A Yusop, M Sinniah, S NatkunamAbstract:A reverse transcription-polymerase chain reaction (RT-PCR) was developed for the detection of Chikungunya virus infection. Based on the nonstructural protein 1 (nsP1) and glycoprotein E1 (E1) genes of Chikungunya, two primer sets were designed. Total RNA were extracted from the cell culture fluid of Aedes albopictus C6/36 cells inoculated with the S27 prototype virus, isolated in Tanzania in 1953, and the Malaysian strains (MALh0198, MALh0298, and MALh0398), isolated in Malaysia in 1998. For both sets of RNA samples, the expected 354- and 294-base pair (bp) cDNA fragments were amplified effectively from the nsP1 and E1 genes, respectively. Phylogenetic analysis was conducted for the Malaysian strain and other virus strains isolated from different regions in the world endemic for Chikungunya, using partial E1 gene sequence data. The Malaysian strains isolated during the epidemics of 1998 fell into a cluster with other members of the Asian genotype.
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combined detection and genotyping of Chikungunya virus by a specific reverse transcription polymerase chain reaction
Journal of Medical Virology, 2002Co-Authors: Futoshi Hasebe, Basu Dev Pandey, Edward Gitau Matumbi Mathenge, Kouichi Morita, V Balasubramaniam, Z Saat, A Yusop, M Sinniah, Maria Del Carmen Parquet, S NatkunamAbstract:A reverse transcription-polymerase chain reaction (RT-PCR) was developed for the detection of Chikungunya virus infection. Based on the nonstructural protein 1 (nsP1) and glycoprotein E1 (E1) genes of Chikungunya, two primer sets were designed. Total RNA were extracted from the cell culture fluid of Aedes albopictus C6/36 cells inoculated with the S27 prototype virus, isolated in Tanzania in 1953, and the Malaysian strains (MALh0198, MALh0298, and MALh0398), isolated in Malaysia in 1998. For both sets of RNA samples, the expected 354- and 294-base pair (bp) cDNA fragments were amplified effectively from the nsP1 and E1 genes, respectively. Phylogenetic analysis was conducted for the Malaysian strain and other virus strains isolated from different regions in the world endemic for Chikungunya, using partial E1 gene sequence data. The Malaysian strains isolated during the epidemics of 1998 fell into a cluster with other members of the Asian genotype. J. Med Virol. 67:370–374, 2002. © 2002 Wiley-Liss, Inc.
Futoshi Hasebe - One of the best experts on this subject based on the ideXlab platform.
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combined detection and genotyping of Chikungunya virus by a specific reverse transcription polymerase chain reaction
Journal of Medical Virology, 2002Co-Authors: Futoshi Hasebe, Maria Del Carmen Parquet, Basu Dev Pandey, Edward Gitau Matumbi Mathenge, Kouichi Morita, V Balasubramaniam, Z Saat, A Yusop, M Sinniah, S NatkunamAbstract:A reverse transcription-polymerase chain reaction (RT-PCR) was developed for the detection of Chikungunya virus infection. Based on the nonstructural protein 1 (nsP1) and glycoprotein E1 (E1) genes of Chikungunya, two primer sets were designed. Total RNA were extracted from the cell culture fluid of Aedes albopictus C6/36 cells inoculated with the S27 prototype virus, isolated in Tanzania in 1953, and the Malaysian strains (MALh0198, MALh0298, and MALh0398), isolated in Malaysia in 1998. For both sets of RNA samples, the expected 354- and 294-base pair (bp) cDNA fragments were amplified effectively from the nsP1 and E1 genes, respectively. Phylogenetic analysis was conducted for the Malaysian strain and other virus strains isolated from different regions in the world endemic for Chikungunya, using partial E1 gene sequence data. The Malaysian strains isolated during the epidemics of 1998 fell into a cluster with other members of the Asian genotype.
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combined detection and genotyping of Chikungunya virus by a specific reverse transcription polymerase chain reaction
Journal of Medical Virology, 2002Co-Authors: Futoshi Hasebe, Basu Dev Pandey, Edward Gitau Matumbi Mathenge, Kouichi Morita, V Balasubramaniam, Z Saat, A Yusop, M Sinniah, Maria Del Carmen Parquet, S NatkunamAbstract:A reverse transcription-polymerase chain reaction (RT-PCR) was developed for the detection of Chikungunya virus infection. Based on the nonstructural protein 1 (nsP1) and glycoprotein E1 (E1) genes of Chikungunya, two primer sets were designed. Total RNA were extracted from the cell culture fluid of Aedes albopictus C6/36 cells inoculated with the S27 prototype virus, isolated in Tanzania in 1953, and the Malaysian strains (MALh0198, MALh0298, and MALh0398), isolated in Malaysia in 1998. For both sets of RNA samples, the expected 354- and 294-base pair (bp) cDNA fragments were amplified effectively from the nsP1 and E1 genes, respectively. Phylogenetic analysis was conducted for the Malaysian strain and other virus strains isolated from different regions in the world endemic for Chikungunya, using partial E1 gene sequence data. The Malaysian strains isolated during the epidemics of 1998 fell into a cluster with other members of the Asian genotype. J. Med Virol. 67:370–374, 2002. © 2002 Wiley-Liss, Inc.
Rémi N. Charrel - One of the best experts on this subject based on the ideXlab platform.
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first cases of autochthonous dengue fever and Chikungunya fever in france from bad dream to reality
Clinical Microbiology and Infection, 2010Co-Authors: Ernest A. Gould, Pierre Gallian, X De Lamballerie, Rémi N. CharrelAbstract:Following the outbreak of autochthonous Chikungunya fever in humans in northern Italy, first reported 3 years ago (1) we have now witnessed two independent cases of autochthonous transmission of dengue fever and two cases of Chikungunya fever in south-eastern France during September 2010. This worrying trend has raised serious concern among the health authorities, as Aedes albopictus, the Asian 'tiger mosquito', is the most likely transmission vector of both viruses, and this mosquito species is known to be spreading widely throughout many regions of southern Europe. The two cases of dengue fever occurred in patients (a 64-year-old male and an 18-year- old male) living in Nice in the same neighbourhood and know- ing each other. The two cases of Chikungunya fever occurred in 12-year-old female patients also living in the vicinity of each other in Frejus, and attending the same high school. Both pre- sented with high fever, headache, lombalgia and arthralgia. These two patients lived in the same neighbourhood as the unique laboratory-documented imported case of the county, affecting a 7-year-old female patient returning from Asia. All four cases resulted in mild, self-resolving infections. The virological identification of these four cases of arbo- viral disease, with no travel history, and within the recorded area of dissemination of A. albopictus, along the 180-km region of the Mediterranean coast from Menton to Toulon (2): (i) demonstrates the first cases of autochthonous trans- mission of both viruses (dengue and Chikungunya) in France; (ii) implies that A. albopictus, which is resident in this region, is the most likely competent vector for dengue virus (http:// www.sante-sports.gouv.fr/apparition-des-premiers-cas-auto chtones-de-dengue-en-france-metropolitaine.html); and (iii) raises important issues to be resolved in time for the next 'mosquito season', namely the adaptation of surveillance systems and the implementation of countermeasures to limit dispersal of the viruses and to limit the number of human cases of dengue or Chikungunya fever. How alarmed should we be in Europe? Imported cases of dengue and Chikungunya fever have long been reported in infected viraemic travellers (3) returning from tropical regions where the viruses are endemic or transiently epi- demic. Indeed, dengue virus ranks second only to the malar- ial parasite as an agent of systemic febrile illness in travellers to the tropics who are returning to Europe (4). However, during the past 5-10 years, the perceived risk of infection in Europe, particularly with these two arboviruses, has increased. This is because a competent mosquito vector was not previously present at a sufficient density in Europe to be able to initiate and generate secondary human cases of Chikungunya or dengue fever among human populations. With the establishment and amplification of A. albopictus in Europe, and these cases of dengue and Chikungunya fever in France, the perceived risk of epidemics caused by these viruses or other emerging arboviruses has increased dramati- cally. Consequently, in an attempt to prevent and monitor the appearance of autochthonous cases, the French authori- ties have elaborated a surveillance programme targeting both viruses in returning travellers during the summer period of vector activity (http://www.circulaires.gouv.fr/pdf/2010/05/cir_
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first cases of autochthonous dengue fever and Chikungunya fever in france from bad dream to reality
Clinical Microbiology and Infection, 2010Co-Authors: Ernest A. Gould, Pierre Gallian, Rémi N. CharrelAbstract:Following the outbreak of autochthonous Chikungunya fever in humans in northern Italy, first reported 3 years ago (1) we have now witnessed two independent cases of autochthonous transmission of dengue fever and two cases of Chikungunya fever in south-eastern France during September 2010. This worrying trend has raised serious concern among the health authorities, as Aedes albopictus, the Asian 'tiger mosquito', is the most likely transmission vector of both viruses, and this mosquito species is known to be spreading widely throughout many regions of southern Europe. The two cases of dengue fever occurred in patients (a 64-year-old male and an 18-year- old male) living in Nice in the same neighbourhood and know- ing each other. The two cases of Chikungunya fever occurred in 12-year-old female patients also living in the vicinity of each other in Frejus, and attending the same high school. Both pre- sented with high fever, headache, lombalgia and arthralgia. These two patients lived in the same neighbourhood as the unique laboratory-documented imported case of the county, affecting a 7-year-old female patient returning from Asia. All four cases resulted in mild, self-resolving infections. The virological identification of these four cases of arbo- viral disease, with no travel history, and within the recorded area of dissemination of A. albopictus, along the 180-km region of the Mediterranean coast from Menton to Toulon (2): (i) demonstrates the first cases of autochthonous trans- mission of both viruses (dengue and Chikungunya) in France; (ii) implies that A. albopictus, which is resident in this region, is the most likely competent vector for dengue virus (http:// www.sante-sports.gouv.fr/apparition-des-premiers-cas-auto chtones-de-dengue-en-france-metropolitaine.html); and (iii) raises important issues to be resolved in time for the next 'mosquito season', namely the adaptation of surveillance systems and the implementation of countermeasures to limit dispersal of the viruses and to limit the number of human cases of dengue or Chikungunya fever. How alarmed should we be in Europe? Imported cases of dengue and Chikungunya fever have long been reported in infected viraemic travellers (3) returning from tropical regions where the viruses are endemic or transiently epi- demic. Indeed, dengue virus ranks second only to the malar- ial parasite as an agent of systemic febrile illness in travellers to the tropics who are returning to Europe (4). However, during the past 5-10 years, the perceived risk of infection in Europe, particularly with these two arboviruses, has increased. This is because a competent mosquito vector was not previously present at a sufficient density in Europe to be able to initiate and generate secondary human cases of Chikungunya or dengue fever among human populations. With the establishment and amplification of A. albopictus in Europe, and these cases of dengue and Chikungunya fever in France, the perceived risk of epidemics caused by these viruses or other emerging arboviruses has increased dramati- cally. Consequently, in an attempt to prevent and monitor the appearance of autochthonous cases, the French authori- ties have elaborated a surveillance programme targeting both viruses in returning travellers during the summer period of vector activity (http://www.circulaires.gouv.fr/pdf/2010/05/cir_
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Chikungunya outbreaks--the globalization of vectorborne diseases.
The New England Journal of Medicine, 2007Co-Authors: Rémi N. Charrel, Xavier De Lamballerie, Didier RaoultAbstract:In 2006, an outbreak of Chikungunya fever swept over a number of islands in the Indian Ocean and India. Drs. Remi Charrel, Xavier de Lamballerie, and Didier Raoult write that the Chikungunya outbreak is an example of the abrupt expression of vectorborne diseases in the global village.
Christophe N. Peyrefitte - One of the best experts on this subject based on the ideXlab platform.
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aedes albopictus mosquito the main vector of the 2007 Chikungunya outbreak in gabon
PLOS ONE, 2009Co-Authors: Frédéric Pagès, Dieudonne Nkoghe, Fanny Jarjaval, Isabelle Iteman, Patrick Gravier, Sylvain Brisse, Christophe N. Peyrefitte, Marc GrandadamAbstract:The primary vector at the origin of the 2007 outbreak in Libreville, Gabon is identified as Aedes albopictus, trapped around the nearby French military camp. The Chikungunya virus was isolated from mosquitoes and found to be identical to the A226V circulating human strain. This is the first field study showing the role of the recently arrived species Aedes albopictus in Chikungunya virus transmission in Central Africa, and it demonstrates this species' role in modifying the epidemiological presentation of Chikungunya in Gabon.
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epidemic resurgence of Chikungunya virus in democratic republic of the congo identification of a new central african strain
Journal of Medical Virology, 2004Co-Authors: Boris Pastorino, Fabienne Tock, J J Muyembetamfum, Hugues Tolou, Mael Bessaud, J P Durand, Christophe N. PeyrefitteAbstract:The resurgence of Chikungunya virus is described during an urban epidemic in Kinshasa Democratic Republic of the Congo, after 39 years without any isolation of the virus. Chikungunya virus was isolated in sera from nine patients with clinical symptoms. A 1,200 bp long partial sequence of the E1/3'UTR genomic region was determined for each isolate. All sequences clustered in the central African lineage. They constitute Chikungunya virus reference sequences for the Democratic Republic of the Congo. J. Med. Virol. 74:277–282, 2004. © 2004 Wiley-Liss, Inc.
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epidemic resurgence of Chikungunya virus in democratic republic of the congo identification of a new central african strain
Journal of Medical Virology, 2004Co-Authors: Boris Pastorino, Fabienne Tock, J J Muyembetamfum, Hugues Tolou, Mael Bessaud, J P Durand, Christophe N. PeyrefitteAbstract:The resurgence of Chikungunya virus is described during an urban epidemic in Kinshasa Democratic Republic of the Congo, after 39 years without any isolation of the virus. Chikungunya virus was isolated in sera from nine patients with clinical symptoms. A 1,200 bp long partial sequence of the E1/3'UTR genomic region was determined for each isolate. All sequences clustered in the central African lineage. They constitute Chikungunya virus reference sequences for the Democratic Republic of the Congo.
A Yusop - One of the best experts on this subject based on the ideXlab platform.
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combined detection and genotyping of Chikungunya virus by a specific reverse transcription polymerase chain reaction
Journal of Medical Virology, 2002Co-Authors: Futoshi Hasebe, Maria Del Carmen Parquet, Basu Dev Pandey, Edward Gitau Matumbi Mathenge, Kouichi Morita, V Balasubramaniam, Z Saat, A Yusop, M Sinniah, S NatkunamAbstract:A reverse transcription-polymerase chain reaction (RT-PCR) was developed for the detection of Chikungunya virus infection. Based on the nonstructural protein 1 (nsP1) and glycoprotein E1 (E1) genes of Chikungunya, two primer sets were designed. Total RNA were extracted from the cell culture fluid of Aedes albopictus C6/36 cells inoculated with the S27 prototype virus, isolated in Tanzania in 1953, and the Malaysian strains (MALh0198, MALh0298, and MALh0398), isolated in Malaysia in 1998. For both sets of RNA samples, the expected 354- and 294-base pair (bp) cDNA fragments were amplified effectively from the nsP1 and E1 genes, respectively. Phylogenetic analysis was conducted for the Malaysian strain and other virus strains isolated from different regions in the world endemic for Chikungunya, using partial E1 gene sequence data. The Malaysian strains isolated during the epidemics of 1998 fell into a cluster with other members of the Asian genotype.
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combined detection and genotyping of Chikungunya virus by a specific reverse transcription polymerase chain reaction
Journal of Medical Virology, 2002Co-Authors: Futoshi Hasebe, Basu Dev Pandey, Edward Gitau Matumbi Mathenge, Kouichi Morita, V Balasubramaniam, Z Saat, A Yusop, M Sinniah, Maria Del Carmen Parquet, S NatkunamAbstract:A reverse transcription-polymerase chain reaction (RT-PCR) was developed for the detection of Chikungunya virus infection. Based on the nonstructural protein 1 (nsP1) and glycoprotein E1 (E1) genes of Chikungunya, two primer sets were designed. Total RNA were extracted from the cell culture fluid of Aedes albopictus C6/36 cells inoculated with the S27 prototype virus, isolated in Tanzania in 1953, and the Malaysian strains (MALh0198, MALh0298, and MALh0398), isolated in Malaysia in 1998. For both sets of RNA samples, the expected 354- and 294-base pair (bp) cDNA fragments were amplified effectively from the nsP1 and E1 genes, respectively. Phylogenetic analysis was conducted for the Malaysian strain and other virus strains isolated from different regions in the world endemic for Chikungunya, using partial E1 gene sequence data. The Malaysian strains isolated during the epidemics of 1998 fell into a cluster with other members of the Asian genotype. J. Med Virol. 67:370–374, 2002. © 2002 Wiley-Liss, Inc.