The Experts below are selected from a list of 231 Experts worldwide ranked by ideXlab platform

Guangjing Chen - One of the best experts on this subject based on the ideXlab platform.

  • influences of different drying methods on the structural characteristics and prebiotic activity of polysaccharides from bamboo shoot Chimonobambusa quadrangularis residues
    International Journal of Biological Macromolecules, 2020
    Co-Authors: Guangjing Chen, Qingyue Hong, Ning Ji, Wenneng Wu
    Abstract:

    Abstract In the present study, in order to assess the influences of drying methods on the chemical structures, in vitro digestibility and prebiotic potential of polysaccharides extracted from Chimonobambusa quadrangularis shoot (CPSs), four drying methods, including hot air-, vacuum-, freeze-, and spray-drying, were utilized to dry CPSs. Results revealed that the physicochemical characteristics and prebiotic activity of CPSs varied by different drying methods. In comparison with the other drying methods, freeze-dried CPSs had higher uronic acid content (9.58%), lower medium-high molecular weight (117.63 kDa), smaller particle size (115.97 nm) and better solubility. All four CPSs fractions exhibited low degree of digestibility to pretended human gastric juice (

  • comparisons of prebiotic activity of polysaccharides from shoot residues of bamboo Chimonobambusa quadrangularis via different ethanol concentrations
    Journal of Food Biochemistry, 2020
    Co-Authors: Guangjing Chen, Changfeng Li, Zhengwei Xiong
    Abstract:

    : Three polysaccharide fractions from bamboo shoot (Chimonobambusa quadrangularis), CPS70, CPS75, and CPS80, were prepared using a final ethanol concentration of 70%, 75%, and 80% in the precipitation process. In vitro digestibility and the prebiotic activity of CPS70, CPS75, and CPS80 were evaluated and compared. The results indicated that all three of the CPS fractions exhibit a high degree of nondigestibility to human gastric juice (>98.5%) or α-amylase hydrolysis (>94.5%). Compared with the blank control, the three CPS fractions could not only significantly (p < .05) stimulate the proliferation of B. adolescentis, B. infantis, B. bifidum, and L. acidophilus, but also significantly (p < .05) enhance the production of lactic, acetic, propionic, and butyric acids when these polysaccharides were added as alternative carbon sources to glucose during the in vitro fermentation of four probiotics. Furthermore, when comparing the three CPS fractions, CPS75 displayed the strongest prebiotic potential, as this polysaccharide had the strongest effect on the proliferation of probiotic bacteria as well as the greatest effect on SCFAs production. These results demonstrated that the concentration of ethanol used during the precipitation process has a significant impact on the prebiotic activity of CPS. PRACTICAL APPLICATIONS: Ethanol precipitation is the first step when extracting polysaccharides from aqueous extracts as it is simple, rapid, and easy to carry out. This study focuses on how different concentrations of ethanol used in the precipitation process affect the prebiotic potential of bamboo shoot (Chimonobambusa quadrangularis) polysaccharides (CPS). The result indicated that the concentration of ethanol used during the precipitation process has a significant impact on the prebiotic activity of CPS. To our knowledge, it is the first to evaluate the effects of the concentration of ethanol during the process of precipitation on prebiotic potential of polysaccharides, which can subsequently be applied to the optimization of ethanol concentration when precipitating natural polysaccharides for the purpose of in vitro fermentation.

  • Comparisons of prebiotic activity of polysaccharides from shoot residues of bamboo (Chimonobambusa quadrangularis) via different ethanol concentrations.
    Journal of Food Biochemistry, 2020
    Co-Authors: Guangjing Chen, Changfeng Li, Zheng‐wei Xiong, Li-zhi Ma
    Abstract:

    : Three polysaccharide fractions from bamboo shoot (Chimonobambusa quadrangularis), CPS70, CPS75, and CPS80, were prepared using a final ethanol concentration of 70%, 75%, and 80% in the precipitation process. In vitro digestibility and the prebiotic activity of CPS70, CPS75, and CPS80 were evaluated and compared. The results indicated that all three of the CPS fractions exhibit a high degree of nondigestibility to human gastric juice (>98.5%) or α-amylase hydrolysis (>94.5%). Compared with the blank control, the three CPS fractions could not only significantly (p 

  • New insight into bamboo shoot (Chimonobambusa quadrangularis) polysaccharides: Impact of extraction processes on its prebiotic activity
    Food Hydrocolloids, 2019
    Co-Authors: Guangjing Chen, Xuhui Chen, Bing Yang, Qingqing Yu, Yongbo Ding
    Abstract:

    The in vitro digestibility, prebiotic activity, and physicochemical properties of polysaccharides obtained from bamboo shoots (Chimonobambusa quadrangularis) using different extraction processes were investigated in this study. Five polysaccharide fractions were prepared by hot water-, accelerated solvent-, ultrasound-, microwave-, and enzyme-based extraction processes. First, the chemical and monosaccharide compositions, molecular weights, zeta potentials, and particle sizes of the five fractions were assessed using colorimetry, high-performance liquid chromatography, high-performance gel permeation chromatography, and dynamic light scattering. Next, digestibility was tested using artificial gastric juice and α-amylase. Finally, the five polysaccharide fractions were added to liquid cultures of bifidobacteria and lactobacilli strains to serve as carbon sources alternative to glucose for in vitro fermentation to evaluate their ability to promote the growth of probiotic bacterial species and stimulate short chain fatty acids (SCFAs) production. It was found polysaccharides obtained by ultrasound- and enzyme-assisted extraction had significantly (p 

  • new insight into bamboo shoot Chimonobambusa quadrangularis polysaccharides impact of extraction processes on its prebiotic activity
    Food Hydrocolloids, 2019
    Co-Authors: Guangjing Chen, Xuhui Chen, Bing Yang, Qingqing Yu, Yongbo Ding
    Abstract:

    The in vitro digestibility, prebiotic activity, and physicochemical properties of polysaccharides obtained from bamboo shoots (Chimonobambusa quadrangularis) using different extraction processes were investigated in this study. Five polysaccharide fractions were prepared by hot water-, accelerated solvent-, ultrasound-, microwave-, and enzyme-based extraction processes. First, the chemical and monosaccharide compositions, molecular weights, zeta potentials, and particle sizes of the five fractions were assessed using colorimetry, high-performance liquid chromatography, high-performance gel permeation chromatography, and dynamic light scattering. Next, digestibility was tested using artificial gastric juice and α-amylase. Finally, the five polysaccharide fractions were added to liquid cultures of bifidobacteria and lactobacilli strains to serve as carbon sources alternative to glucose for in vitro fermentation to evaluate their ability to promote the growth of probiotic bacterial species and stimulate short chain fatty acids (SCFAs) production. It was found polysaccharides obtained by ultrasound- and enzyme-assisted extraction had significantly (p < 0.05) higher total carbohydrate contents of 61.9% and 61.5%, lower medium-high MWs of 117.49 and 117.25 kDa, and smaller particle sizes of 116.8 and 190.73 nm, respectively. All of the polysaccharide fractions were resistant to hydrolysis by artificial gastric juice and α-amylase. Furthermore, the five polysaccharides significantly stimulated the growth of the tested probiotics and increased lactic, acetate, propionate, and butyrate acids production from fermentation for 48 h compared to the control (p < 0.05). The polysaccharides prepared using ultrasound- and enzyme-assisted extraction processes displayed better prebiotic activity than the other fractions as they induced notably more proliferation of probiotic bacteria and higher production of SCFAs.

Shasha Wang - One of the best experts on this subject based on the ideXlab platform.

  • characterization of physicochemical properties and antioxidant activity of polysaccharides from shoot residues of bamboo Chimonobambusa quadrangularis effect of drying procedures
    Food Chemistry, 2019
    Co-Authors: Guangjing Chen, Changfeng Li, Shasha Wang, Hongxin Zhang
    Abstract:

    Abstract In the present study, the bamboo shoot (Chimonobambusa quadrangularis) residue (BSR)-derived polysaccharides (CPS) were dried using different drying procedures, including hot air-, vacuum-, freeze-, and spray-drying. The influences of different drying procedures on the chemical compositions, physicochemical characteristics, and antioxidant activity of CPS were investigated. The results indicate that freeze-dried CPS possessed the highest polysaccharide yield (8.86%) and uronic acid content (9.42%), and the lowest medium-high molecular weight (117.49 kDa) and glucose content (5.81 mol%). Also, freeze-dried CPS had better water (5.17 g/g) and oil retention capacities (2.32 g/g) than hot air- and vacuum-dried CPS. Moreover, freeze-dried CPS exhibited the most potent antioxidant ability. Based on correlation analysis, a higher uronic acid content, lower molecular weight and lower glucose content might be closely associated with the better antioxidant activity of the freeze-dried CPS. Hence, the above results revealed freeze-drying was the optimal method for industrial production of high quality CPS.

  • ultrasonic extraction structural characterization physicochemical properties and antioxidant activities of polysaccharides from bamboo shoots Chimonobambusa quadrangularis processing by products
    International Journal of Biological Macromolecules, 2018
    Co-Authors: Guangjing Chen, Changfeng Li, Xuhui Chen, Fan Bu, Shasha Wang
    Abstract:

    Abstract In this study, a novel polysaccharide (PCPs) was obtained from bamboo shoots (Chimonobambusa quadrangularis) processing by-products through the ultrasonic-assisted extraction (UAE) and Sephadex G-100 size-exclusion chromatography purification. By using a Box–Behnken design (BBD) to optimize the parameters for extracting the crude PCPs with UAE, the maximum yield of crude PCPs (8.76%) was obtained under the optimal extraction conditions as follows: ratio of water to raw material 20.2 mL/g, extraction temperature 49 °C, ultrasonic power 240 W, and extraction time 40 min. Structurally, PCPs was identified as hetero-polysaccharides, which was composed of Man, Rha, GlcA, GalA, Glc, Gal, Xyl, and Ara in a molecular ratio of 1.00:2.05:2.28:2.36:15.76:49.06:16.70:39.86. The molecular weight distribution analysis showed that PCPs had three components with molecular weights of 136.1, 29.7 and 8.3 kD, respectively. The glycosidic linkage analysis showed that PCPs contained 1 → or 1 → 6 glycosidic linkages (35.1%), 1 → 2 or 1 → 4 glycosidic linkages (14.8%), and 1 → 3 glycosidic linkages (50.1%). conformations. The obtained results revealed that PCPs with a semi-crystalline structure exhibited important functional properties including the repose angle, swelling index (SC), water retention capacity (WRC), and oil retention capacity (ORC). The viscoelastic property of PCPs system exhibited a gel-like behavior with storage modulus G′ always being higher than loss modulus G″. PCPs displayed excellent thermal stability as assessed by rheology and TGA/DSC. Furthermore, PCPs exhibited good chemical antioxidant activities in DPPH, ABTS and hydroxyl radical scavenging abilities, and metal chelating activity.

Hongxin Zhang - One of the best experts on this subject based on the ideXlab platform.

  • characterization of physicochemical properties and antioxidant activity of polysaccharides from shoot residues of bamboo Chimonobambusa quadrangularis effect of drying procedures
    Food Chemistry, 2019
    Co-Authors: Guangjing Chen, Changfeng Li, Shasha Wang, Hongxin Zhang
    Abstract:

    Abstract In the present study, the bamboo shoot (Chimonobambusa quadrangularis) residue (BSR)-derived polysaccharides (CPS) were dried using different drying procedures, including hot air-, vacuum-, freeze-, and spray-drying. The influences of different drying procedures on the chemical compositions, physicochemical characteristics, and antioxidant activity of CPS were investigated. The results indicate that freeze-dried CPS possessed the highest polysaccharide yield (8.86%) and uronic acid content (9.42%), and the lowest medium-high molecular weight (117.49 kDa) and glucose content (5.81 mol%). Also, freeze-dried CPS had better water (5.17 g/g) and oil retention capacities (2.32 g/g) than hot air- and vacuum-dried CPS. Moreover, freeze-dried CPS exhibited the most potent antioxidant ability. Based on correlation analysis, a higher uronic acid content, lower molecular weight and lower glucose content might be closely associated with the better antioxidant activity of the freeze-dried CPS. Hence, the above results revealed freeze-drying was the optimal method for industrial production of high quality CPS.

Changfeng Li - One of the best experts on this subject based on the ideXlab platform.

  • comparisons of prebiotic activity of polysaccharides from shoot residues of bamboo Chimonobambusa quadrangularis via different ethanol concentrations
    Journal of Food Biochemistry, 2020
    Co-Authors: Guangjing Chen, Changfeng Li, Zhengwei Xiong
    Abstract:

    : Three polysaccharide fractions from bamboo shoot (Chimonobambusa quadrangularis), CPS70, CPS75, and CPS80, were prepared using a final ethanol concentration of 70%, 75%, and 80% in the precipitation process. In vitro digestibility and the prebiotic activity of CPS70, CPS75, and CPS80 were evaluated and compared. The results indicated that all three of the CPS fractions exhibit a high degree of nondigestibility to human gastric juice (>98.5%) or α-amylase hydrolysis (>94.5%). Compared with the blank control, the three CPS fractions could not only significantly (p < .05) stimulate the proliferation of B. adolescentis, B. infantis, B. bifidum, and L. acidophilus, but also significantly (p < .05) enhance the production of lactic, acetic, propionic, and butyric acids when these polysaccharides were added as alternative carbon sources to glucose during the in vitro fermentation of four probiotics. Furthermore, when comparing the three CPS fractions, CPS75 displayed the strongest prebiotic potential, as this polysaccharide had the strongest effect on the proliferation of probiotic bacteria as well as the greatest effect on SCFAs production. These results demonstrated that the concentration of ethanol used during the precipitation process has a significant impact on the prebiotic activity of CPS. PRACTICAL APPLICATIONS: Ethanol precipitation is the first step when extracting polysaccharides from aqueous extracts as it is simple, rapid, and easy to carry out. This study focuses on how different concentrations of ethanol used in the precipitation process affect the prebiotic potential of bamboo shoot (Chimonobambusa quadrangularis) polysaccharides (CPS). The result indicated that the concentration of ethanol used during the precipitation process has a significant impact on the prebiotic activity of CPS. To our knowledge, it is the first to evaluate the effects of the concentration of ethanol during the process of precipitation on prebiotic potential of polysaccharides, which can subsequently be applied to the optimization of ethanol concentration when precipitating natural polysaccharides for the purpose of in vitro fermentation.

  • Comparisons of prebiotic activity of polysaccharides from shoot residues of bamboo (Chimonobambusa quadrangularis) via different ethanol concentrations.
    Journal of Food Biochemistry, 2020
    Co-Authors: Guangjing Chen, Changfeng Li, Zheng‐wei Xiong, Li-zhi Ma
    Abstract:

    : Three polysaccharide fractions from bamboo shoot (Chimonobambusa quadrangularis), CPS70, CPS75, and CPS80, were prepared using a final ethanol concentration of 70%, 75%, and 80% in the precipitation process. In vitro digestibility and the prebiotic activity of CPS70, CPS75, and CPS80 were evaluated and compared. The results indicated that all three of the CPS fractions exhibit a high degree of nondigestibility to human gastric juice (>98.5%) or α-amylase hydrolysis (>94.5%). Compared with the blank control, the three CPS fractions could not only significantly (p 

  • characterization of physicochemical properties and antioxidant activity of polysaccharides from shoot residues of bamboo Chimonobambusa quadrangularis effect of drying procedures
    Food Chemistry, 2019
    Co-Authors: Guangjing Chen, Changfeng Li, Shasha Wang, Hongxin Zhang
    Abstract:

    Abstract In the present study, the bamboo shoot (Chimonobambusa quadrangularis) residue (BSR)-derived polysaccharides (CPS) were dried using different drying procedures, including hot air-, vacuum-, freeze-, and spray-drying. The influences of different drying procedures on the chemical compositions, physicochemical characteristics, and antioxidant activity of CPS were investigated. The results indicate that freeze-dried CPS possessed the highest polysaccharide yield (8.86%) and uronic acid content (9.42%), and the lowest medium-high molecular weight (117.49 kDa) and glucose content (5.81 mol%). Also, freeze-dried CPS had better water (5.17 g/g) and oil retention capacities (2.32 g/g) than hot air- and vacuum-dried CPS. Moreover, freeze-dried CPS exhibited the most potent antioxidant ability. Based on correlation analysis, a higher uronic acid content, lower molecular weight and lower glucose content might be closely associated with the better antioxidant activity of the freeze-dried CPS. Hence, the above results revealed freeze-drying was the optimal method for industrial production of high quality CPS.

  • ultrasonic extraction structural characterization physicochemical properties and antioxidant activities of polysaccharides from bamboo shoots Chimonobambusa quadrangularis processing by products
    International Journal of Biological Macromolecules, 2018
    Co-Authors: Guangjing Chen, Changfeng Li, Xuhui Chen, Fan Bu, Shasha Wang
    Abstract:

    Abstract In this study, a novel polysaccharide (PCPs) was obtained from bamboo shoots (Chimonobambusa quadrangularis) processing by-products through the ultrasonic-assisted extraction (UAE) and Sephadex G-100 size-exclusion chromatography purification. By using a Box–Behnken design (BBD) to optimize the parameters for extracting the crude PCPs with UAE, the maximum yield of crude PCPs (8.76%) was obtained under the optimal extraction conditions as follows: ratio of water to raw material 20.2 mL/g, extraction temperature 49 °C, ultrasonic power 240 W, and extraction time 40 min. Structurally, PCPs was identified as hetero-polysaccharides, which was composed of Man, Rha, GlcA, GalA, Glc, Gal, Xyl, and Ara in a molecular ratio of 1.00:2.05:2.28:2.36:15.76:49.06:16.70:39.86. The molecular weight distribution analysis showed that PCPs had three components with molecular weights of 136.1, 29.7 and 8.3 kD, respectively. The glycosidic linkage analysis showed that PCPs contained 1 → or 1 → 6 glycosidic linkages (35.1%), 1 → 2 or 1 → 4 glycosidic linkages (14.8%), and 1 → 3 glycosidic linkages (50.1%). conformations. The obtained results revealed that PCPs with a semi-crystalline structure exhibited important functional properties including the repose angle, swelling index (SC), water retention capacity (WRC), and oil retention capacity (ORC). The viscoelastic property of PCPs system exhibited a gel-like behavior with storage modulus G′ always being higher than loss modulus G″. PCPs displayed excellent thermal stability as assessed by rheology and TGA/DSC. Furthermore, PCPs exhibited good chemical antioxidant activities in DPPH, ABTS and hydroxyl radical scavenging abilities, and metal chelating activity.

Yongbo Ding - One of the best experts on this subject based on the ideXlab platform.

  • New insight into bamboo shoot (Chimonobambusa quadrangularis) polysaccharides: Impact of extraction processes on its prebiotic activity
    Food Hydrocolloids, 2019
    Co-Authors: Guangjing Chen, Xuhui Chen, Bing Yang, Qingqing Yu, Yongbo Ding
    Abstract:

    The in vitro digestibility, prebiotic activity, and physicochemical properties of polysaccharides obtained from bamboo shoots (Chimonobambusa quadrangularis) using different extraction processes were investigated in this study. Five polysaccharide fractions were prepared by hot water-, accelerated solvent-, ultrasound-, microwave-, and enzyme-based extraction processes. First, the chemical and monosaccharide compositions, molecular weights, zeta potentials, and particle sizes of the five fractions were assessed using colorimetry, high-performance liquid chromatography, high-performance gel permeation chromatography, and dynamic light scattering. Next, digestibility was tested using artificial gastric juice and α-amylase. Finally, the five polysaccharide fractions were added to liquid cultures of bifidobacteria and lactobacilli strains to serve as carbon sources alternative to glucose for in vitro fermentation to evaluate their ability to promote the growth of probiotic bacterial species and stimulate short chain fatty acids (SCFAs) production. It was found polysaccharides obtained by ultrasound- and enzyme-assisted extraction had significantly (p 

  • new insight into bamboo shoot Chimonobambusa quadrangularis polysaccharides impact of extraction processes on its prebiotic activity
    Food Hydrocolloids, 2019
    Co-Authors: Guangjing Chen, Xuhui Chen, Bing Yang, Qingqing Yu, Yongbo Ding
    Abstract:

    The in vitro digestibility, prebiotic activity, and physicochemical properties of polysaccharides obtained from bamboo shoots (Chimonobambusa quadrangularis) using different extraction processes were investigated in this study. Five polysaccharide fractions were prepared by hot water-, accelerated solvent-, ultrasound-, microwave-, and enzyme-based extraction processes. First, the chemical and monosaccharide compositions, molecular weights, zeta potentials, and particle sizes of the five fractions were assessed using colorimetry, high-performance liquid chromatography, high-performance gel permeation chromatography, and dynamic light scattering. Next, digestibility was tested using artificial gastric juice and α-amylase. Finally, the five polysaccharide fractions were added to liquid cultures of bifidobacteria and lactobacilli strains to serve as carbon sources alternative to glucose for in vitro fermentation to evaluate their ability to promote the growth of probiotic bacterial species and stimulate short chain fatty acids (SCFAs) production. It was found polysaccharides obtained by ultrasound- and enzyme-assisted extraction had significantly (p < 0.05) higher total carbohydrate contents of 61.9% and 61.5%, lower medium-high MWs of 117.49 and 117.25 kDa, and smaller particle sizes of 116.8 and 190.73 nm, respectively. All of the polysaccharide fractions were resistant to hydrolysis by artificial gastric juice and α-amylase. Furthermore, the five polysaccharides significantly stimulated the growth of the tested probiotics and increased lactic, acetate, propionate, and butyrate acids production from fermentation for 48 h compared to the control (p < 0.05). The polysaccharides prepared using ultrasound- and enzyme-assisted extraction processes displayed better prebiotic activity than the other fractions as they induced notably more proliferation of probiotic bacteria and higher production of SCFAs.

  • polysaccharides obtained from bamboo shoots Chimonobambusa quadrangularis processing by products new insight into ethanol precipitation and characterization
    International Journal of Biological Macromolecules, 2018
    Co-Authors: Fusheng Zhang, Yongbo Ding, Jiong Zheng, Guangjing Chen
    Abstract:

    Abstract Chimonobambusa quadrangularis polysaccharides (CPS) were extracted by ultrasonic-assisted extraction from bamboo shoots ( C . quadrangularis ) processing by-products. Three polysaccharide fractions, CPS70, CPS75 and CPS80, were obtained by precipitation at final ethanol concentrations of 70%, 75% and 80%, respectively. The physicochemical characterization and chemical antioxidant activities of the three polysaccharide fractions were compared on the basis of HPLC, FT-IR, XRD, TGA, and antioxidant measurements in vitro . The results suggested that ethanol concentrations used for precipitation of CPS can affect its physicochemical and associated functional properties, and antioxidant activities. Compared with CPS70 and CPS80, CPS75 had lower glucose content, higher total sugar content, and higher protein and uronic acid contents. The CPS70 and CPS80 were composed of Man, Rha, GlcA, Glc, Gal, Xyl and Ara, but none of them were found to contain GalA. In contrast, CPS75 consisted of Man, Rha, GlcA, GalA, Glc, Gal, Xyl and Ara. CPS75 had the lowest medium-high-molecular-weight value (116.53–118.18 kDa) and the highest medium-low-molecular-weight value (21.30–22.68 kDa). Meanwhile, CPS75 exhibited better functional properties including the repose angle, swelling capacity (SC), water retention capacity (WRC), and oil retention capacity (ORC). Moreover, CPS75 possessed higher scavenging capacities on DPPH, hydroxyl and ABTS radicals, higher oxygen radical absorbance capacity (OARC), higher metal chelating activity, and more significant reducing power. According to the results above, a final ethanol concentration of 75% could be chose to precipitate polysaccharides from bamboo shoots ( C . quadrangularis ) processing by-products. In summary, it is strongly recommended that the ethanol concentration employed in precipitation of natural polysaccharides could be optimized in advance.