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Norbert A. Dencher - One of the best experts on this subject based on the ideXlab platform.
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Metabolism controls dimerization of the chloroplast FoF1 ATP synthase in Chlamydomonas reinhardtii
FEBS Letters, 2007Co-Authors: Helena J. Schwaßmann, Sascha Rexroth, Holger Seelert, Norbert A. DencherAbstract:Abstract Dimers and oligomers of F-type ATP synthases have been observed previously in mitochondria of various organisms and for the CFoF1 ATP synthase of chloroplasts of Chlamydomonas reinhardtii. In contrast to mitochondria, however, dimers of chloroplast ATP synthases dissociate at elevated phosphate concentration. This suggests a regulation by cell physiological processes. Stable isotope labeling of living cells and blue-native PAGE have been employed to quantitate changes in the ratio of monomeric to dimeric CFoF1 ATP synthase. Chlamydomonas reinhardtii cells were cultivated photoautotrophically in the presence of 15N and photomixotrophically at natural 14N abundance, respectively. As compared to photoautotrophic growth, an increased assembly of ATP synthase dimers on the expense of preexisting monomers during photomixotrophic growth was observed, demonstrating a metabolic control of the dimerization process.
Christoph F. Beck - One of the best experts on this subject based on the ideXlab platform.
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Identification of novel genes specifically expressed in Chlamydomonas reinhardtii zygotes
Plant Molecular Biology, 1991Co-Authors: Dorothee Wegener, Christoph F. BeckAbstract:The maturation of zygotes formed by the fusion of two gametes is the essential part of the diploid phase of the Chlamydomonas reinhardtii sexual life cycle and results in mature zygotes competent to germinate. To understand the molecular mechanisms underlying zygote maturation and the attainment of competence for germination we isolated genomic clones representing three different genes that are specifically expressed in Chlamydomonas reinhardtii zygotes. Accumulation of the RNAs started more than 24 h after mating, setting these genes apart from genes expressed in young zygotes [9]. Upon light-induced germination of zygotes, the mRNAs disappeared. The patterns of RNA accumulation and disappearance were gene-specific and suggested a function of these genes in maturation and/or in initial steps of germination.
Akio Kobayashi - One of the best experts on this subject based on the ideXlab platform.
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introduction of the archaebacterial geranylgeranyl pyrophosphate synthase gene into Chlamydomonas reinhardtii chloroplast
Journal of Bioscience and Bioengineering, 2003Co-Authors: Eiichiro Fukusaki, Atsuhiko Shinmyo, Ko Kato, Tokuzo Nishino, Hisashi Hemmi, Takashi Nishikawa, Akio KobayashiAbstract:Geranylgeranyl pyrophosphate (GGPP) synthase gene (gds) derived from a thermophilic Archae Sulfolobus acidocaldarius, was introduced into a unicellular green alga Chlamydomonas reinhardtii chloroplast. Heat treatment abolished the prenyltransferase activity of the wild strain, but the activity of the transforment remained. The transformant accumulated gds gene mRNA and translation product.
Laura R. Keller - One of the best experts on this subject based on the ideXlab platform.
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Introduction of exogenous DNA into Chlamydomonas reinhardtii by electroporation.
Molecular and cellular biology, 1991Co-Authors: L. E. Brown, S. L. Sprecher, Laura R. KellerAbstract:The fate of exogenous DNA introduced into Chlamydomonas reinhardtii by electroporation was analyzed. With single and double electrical pulses, plasmids as large as 14 kb were introduced into cells with and without intact cell walls. Within hours after introduction, exogenous plasmid DNA was associated with nuclei isolated from cells; several weeks after introduction, exogenous DNA was stably integrated into the Chlamydomonas genome. These studies establish electroporation as a method for introducing DNA, and potentially other molecules, into C. reinhardtii.
Helena J. Schwaßmann - One of the best experts on this subject based on the ideXlab platform.
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Metabolism controls dimerization of the chloroplast FoF1 ATP synthase in Chlamydomonas reinhardtii
FEBS Letters, 2007Co-Authors: Helena J. Schwaßmann, Sascha Rexroth, Holger Seelert, Norbert A. DencherAbstract:Abstract Dimers and oligomers of F-type ATP synthases have been observed previously in mitochondria of various organisms and for the CFoF1 ATP synthase of chloroplasts of Chlamydomonas reinhardtii. In contrast to mitochondria, however, dimers of chloroplast ATP synthases dissociate at elevated phosphate concentration. This suggests a regulation by cell physiological processes. Stable isotope labeling of living cells and blue-native PAGE have been employed to quantitate changes in the ratio of monomeric to dimeric CFoF1 ATP synthase. Chlamydomonas reinhardtii cells were cultivated photoautotrophically in the presence of 15N and photomixotrophically at natural 14N abundance, respectively. As compared to photoautotrophic growth, an increased assembly of ATP synthase dimers on the expense of preexisting monomers during photomixotrophic growth was observed, demonstrating a metabolic control of the dimerization process.