The Experts below are selected from a list of 360 Experts worldwide ranked by ideXlab platform
Shulian Xie - One of the best experts on this subject based on the ideXlab platform.
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molecular mechanism of lipid accumulation and metabolism of oleaginous Chlorococcum sphacosum gd from soil under salt stress
International Journal of Molecular Sciences, 2021Co-Authors: Jia Feng, Qi Liu, Fangru Nan, Xudong Liu, Shulian XieAbstract:The oleaginous microalgae species Chlorococcum sphacosum GD is a promising feedstock for biodiesel production from soil. However, its metabolic mechanism of lipid production remains unclear. In this study, the lipid accumulation and metabolism mechanisms of Chlorococcum sphacosum GD were analyzed under salt stress based on transcriptome sequencing. The biomass and lipid content of the alga strain were determined under different NaCl concentrations, and total RNA from fresh cells were isolated and sequenced by HiSeq 2000 high throughput sequencing technology. As the salt concentration increased in culture medium, the algal lipid content increased but the biomass decreased. Following transcriptome sequencing by assembly and splicing, 24,128 unigenes were annotated, with read lengths mostly distributed in the 200–300 bp interval. Statistically significant differentially expressed unigenes were observed in different experimental groups, with 2051 up-regulated genes and 1835 down-regulated genes. The lipid metabolism pathway analysis showed that, under salt stress, gene-related fatty acid biosynthesis (ACCase, KASII, KAR, HAD, FATA) was significantly up-regulated, but some gene-related fatty acid degradation was significantly down-regulated. The comprehensive results showed that salt concentration can affect the lipid accumulation and metabolism of C. sphacosum GD, and the lipid accumulation is closely related to the fatty acid synthesis pathway.
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transcriptomic analysis reveals the mechanism on the response of Chlorococcum sp gd to glucose concentration in mixotrophic cultivation
Bioresource Technology, 2019Co-Authors: Fei Zhao, Jia Feng, Qi Liu, Fangru Nan, Xudong Liu, Shulian XieAbstract:Abstract In this study, the performance of Chlorococcum sp. GD in synthetic medium with different glucose concentrations (ranging from 1 to10 g/L) was investigated. Moreover, transcriptome sequencing was conducted to clarify the response of the microalga to glucose concentrations. High concentration of glucose (6–10 g/L) not only did not provide a higher yield of biomass but also inhibited photosynthesis. Transcriptomic analysis revealed that the glucose metabolism mainly depended on the glycolysis and the pentose phosphate pathway (PPP) as the microalga was cultivated with 10 g/L glucose. Meanwhile the tricarboxylic acid (TCA) cycle, oxidative phosphorylation and photosynthesis were significantly inhibited. The significant change on carbon metabolic flux caused by the increase in glucose concentration affected the synthesis of reducing power and ATP, which ultimately influenced the growth of the microalga. Appropriate supplement of organic carbon not only enhances the biomass accumulation but also increases the utilization efficiency of organic carbon.
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the performance of a self flocculating microalga Chlorococcum sp gd in wastewater with different ammonia concentrations
International Journal of Environmental Research and Public Health, 2018Co-Authors: Xuechun Wang, Jia Feng, Qi Liu, Wei Liu, Fangru Nan, Xiaoyan Jiao, Shulian XieAbstract:The performance of a self-flocculating microalga Chlorococcum sp. GD on the flocculation, growth, and lipid accumulation in wastewater with different ammonia nitrogen concentrations was investigated. It was revealed that relative high ammonia nitrogen concentration (20–50 mg·L−1) was beneficial to the flocculation of Chlorococcum sp. GD, and the highest flocculating efficiency was up to 84.4%. It was also found that the highest flocculating efficiency occurred in the middle of the culture (4–5 days) regardless of initial ammonia concentration in wastewater. It was speculated that high flocculating efficiency was likely related to the production of extracellular proteins. 20 mg·L−1 of ammonia was found to be a preferred concentration for both biomass production and lipid accumulation. 92.8% COD, 98.8% ammonia, and 69.4% phosphorus were removed when Chlorococcum sp. GD was cultivated in wastewater with 20 mg·L−1 ammonia. The novelty and significance of the investigation was the integration of flocculation, biomass production, wastewater treatment, and lipid accumulation, simultaneously, which made Chlorococcum sp. GD a potential candidate for wastewater treatment and biodiesel production if harvested in wastewater with suitable ammonia nitrogen concentration.
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effect of sulfate ions on growth and pollutants removal of self flocculating microalga Chlorococcum sp gd in synthetic municipal wastewater
Bioresource Technology, 2017Co-Authors: Junyan Guo, Jia Feng, Qi Liu, Shulian XieAbstract:Abstract Sulfate is a primary sulfur source and can be available in wastewaters. Nevertheless, effect of sulfate ions on growth and pollutants removal of microalgae seems to be less investigated. At the present study, self-flocculating microalga Chlorococcum sp. GD was grown in synthetic municipal wastewater with different sulfate concentrations. Results indicated that Chlorococcum sp. GD grew better in synthetic municipal wastewater with 18, 45, 77, 136 and 271 mg/L SO 4 2− than in wastewater without SO 4 2− . Chlorococcum sp. GD had also excellent removal efficiencies of nitrogen and phosphorus and effectively flocculated in sulfate wastewater. Sulfate deprivation weakened the growth, pollutants removal and self-flocculation of Chlorococcum sp. GD in wastewater. Antioxidative enzymes activity significantly increased and photosynthetic activity significantly decreased when Chlorococcum sp. GD was cultivated in sulfate-free wastewater. Sulfate deprivation probably reduced cell activity of growth, pollutants removal and flocculation via inducing the over-accumulation of reactive oxygen species (ROS).
Xiujuan Chen - One of the best experts on this subject based on the ideXlab platform.
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molecular toxicity of triclosan and carbamazepine to green algae Chlorococcum sp a single cell view using synchrotron based fourier transform infrared spectromicroscopy
Environmental Pollution, 2017Co-Authors: Xiaying Xin, G H Huang, Harold G Weger, Xia Liu, Yao Yao, Peng Zhang, Xiujuan ChenAbstract:Although pharmaceuticals and personal care products have been used and introduced into the environment in large quantities, little information on potential ecological risks is currently available considering their effects on living organisms. We verified the feasibility of using synchrotron-based Fourier Transform Infrared (SR-FTIR) spectromicroscopy to explore in vivo toxic effects on single living Chlorococcum sp. cells. The study provided important information to achieve a better understanding of the toxic mechanism of triclosan and carbamazepine on living algae Chlorococcum sp.. Triclosan and carbamazepine had distinctive toxic effects on unicellular living algae. Most strikingly, triclosan had more dramatic toxic effects on biochemical components than carbamazepine. Triclosan can affect algae primarily by inhibiting fatty acid synthesis and causing protein aggregation. The toxicity response was irreversible at higher concentration (100.000 μM), but attenuated at lower concentration (0.391 μM) as time extended. Carbamazepine can produce hydrophobic interactions to affect the phospholipid bilayer and work on specific proteins to disfunction the cell membrane. Carbamazepine-exposed cells developed a resistance while extending exposure time. This is the first demonstration from an ecological standpoint that SR-FTIR can provide an innovative approach to reveal the toxicity of emerging pollutants in aquatic environments.
Yuankun Lee - One of the best experts on this subject based on the ideXlab platform.
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effect of total secondary carotenoids extracts from Chlorococcum sp on helicobacter pylori infected balb c mice
International Immunopharmacology, 2003Co-Authors: B H Liu, Yuankun LeeAbstract:Abstract Helicobacter pylori is a human pathogen associated with type B gastritis, peptic ulcer disease and gastric cancer. A high intake of carotenoids has been suggested to prevent development of gastric malignancies. Microalgae Chlorococcum sp. could accumulate secondary carotenoids under stress conditions. The aim of the present study was to investigate whether dietary cell extract of Chlorococcum sp. could affect the bacterial load of H. pylori infected BALB/c mice and whether it could induce modulation of cytokine production. BALB/c mice were infected with H. pylori three times at 2-day intervals. Two weeks later, they were orally fed with cell extract of Chlorococcum sp. for the following 2 weeks. Animals were killed at the end of the experiments. Stomachs were removed and paraffin sections were stained for histology examination. Splenocytes were obtained and cultured in vitro with H. pylori sonicate to evaluate induction of interferon gamma (IFN-γ) and interleukin 4 (IL-4) secretion. Mice treated with carotenoids-rich algal meal showed significantly reduced bacterial load and gastric inflammation. These changes were associated with a shift of the T-lymphocytes response from a predominant T helper type 1 (Th1) response dominated by IFN-γ to a Th1/Th2 response with IFN-γ and IL-4.
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secondary carotenoids formation by the green alga Chlorococcum sp
Journal of Applied Phycology, 2000Co-Authors: Beihui Liu, Yuankun LeeAbstract:The effects of temperature, pH and oxygen level onsecondary carotenoids (SC) accumulation in Chlorococcum sp. were investigated. The optimaltemperature, pH and oxygen level for the yields ofsecondary carotenoids and astaxanthin were35 °C, pH 8 and 10% (v/v), respectively. Whilethe ratio (R) of products containing hydroxyl group(s) to those containing keto group (s) increased withthe increase of temperature and pH, R value decreasedwith the level of oxygen. These results indicate thathigher temperature and pH favor the introduction ofhydroxyl group to the corresponding substrates,however, under high oxygen level keto group wasrelatively easy to be added compared to the additionof hydroxyl.
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composition and biosynthetic pathways of carotenoids in the astaxanthin producing green alga Chlorococcum sp
Biotechnology Letters, 1999Co-Authors: Beihui Liu, Yuankun LeeAbstract:The composition of the secondary carotenoids in the astaxanthin-producing green alga Chlorococcum sp. was analyzed. Eight types of carotenoids were identified by a variety of spectroscopic techniques. Canthaxanthin, 3′-hydroxyechinenone, adonirubin and adonixanthin comprised, respectively, 32%, 23%, 12% and 9% of total carotenoids. The results imply that Chlorococcum sp. synthesized astaxanthin from β-carotene through various pathways which are different from other astaxanthin-producing microorganisms.
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ketocarotenoid production by a mutant of Chlorococcum sp in an outdoor tubular photobioreactor
Biotechnology Letters, 1999Co-Authors: Daohai Zhang, Yuankun LeeAbstract:A mutant, MA-1, of Chlorococcum sp., grown in batch culture, produced about 54 mg ketocarotenoids/l with 10 mM nitrogen. The accumulation rate of these ketocarotenoids was independent of the nitrogen concentration under sunlight illumination. Fed-batch cultures showed poor growth and the average productivity of ketocarotenoids dropped from 2.6 mg/l day to 1.6 mg/l day in the two consecutive fed-batch runs.
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enhanced accumulation of secondary carotenoids in a mutant of the green alga Chlorococcum sp
Journal of Applied Phycology, 1997Co-Authors: Dao Hua Zhang, Yuankun LeeAbstract:A colour mutant of the unicellular green alga Chlorococcum sp. was obtained by visual colour detection method on plates with medium containing sodium azide. The growth of the mutant MA-1 was more susceptible to azide compared with the wild type, whereas the total secondary carotenoid (SC)synthesis was more resistant to the inhibitor. The azide concentration that inhibited SC formation by 50% (I50) was ten times higher than that required for the wild type. The mutant was stable over several consecutive subculturings in the absence of azide. The indoor and outdoor studies showed that the mutant could synthesise more than 2-fold of the total SC and astaxanthin of the wild type. The mutant MA-1 could be a natural source of SC and astaxanthin.
Xiaying Xin - One of the best experts on this subject based on the ideXlab platform.
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insights into the toxicity of triclosan to green microalga Chlorococcum sp using synchrotron based fourier transform infrared spectromicroscopy biophysiological analyses and roles of environmental factors
Environmental Science & Technology, 2018Co-Authors: Xiaying Xin, G H Huang, Charley Huang, Harold G Weger, Shan Zhao, Yang Zhou, Scott M RosendahlAbstract:This study investigated the toxicity of triclosan to the green microalga Chlorococcum sp. under multiple environmental stressors. The interactions between triclosan and environmental stressors were explored through full two-way factorial, synchrotron-based Fourier transform infrared spectromicroscopy and principal component analyses. Phosphorus concentration, pH * phosphorus concentration, and temperature * pH * NaCl concentration were the most statistically significant factors under triclosan exposure. The variation of those factors would have a huge impact on biophysiological performances. It is interesting to find Chlorococcum sp. may become more resistant against triclosan in phosphorus-enriched environment. Besides, particular significant factors from multiple environmental stressors showed the impacts of triclosan on the corresponding response of Chlorococcum sp. owing to the specific structure and performance of biomolecular components. Moreover, two high-order interactions of temperature * pH * NaCl concentration and temperature * pH * NaCl concentration * phosphorus concentration had more contributions than others at the subcellular level, which could be attributed to the interactive complexity of biomolecular components. Due to cellular self-regulation mechanism and short exposure time, the biophysiological changes of Chlorococcum sp. were undramatic. These findings can help reveal the interactive complexity among triclosan and multiple environmental stressors. It is suggested that multiple environmental stressors should be considered during ecological risk assessment and management of emerging pollutants.
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molecular toxicity of triclosan and carbamazepine to green algae Chlorococcum sp a single cell view using synchrotron based fourier transform infrared spectromicroscopy
Environmental Pollution, 2017Co-Authors: Xiaying Xin, G H Huang, Harold G Weger, Xia Liu, Yao Yao, Peng Zhang, Xiujuan ChenAbstract:Although pharmaceuticals and personal care products have been used and introduced into the environment in large quantities, little information on potential ecological risks is currently available considering their effects on living organisms. We verified the feasibility of using synchrotron-based Fourier Transform Infrared (SR-FTIR) spectromicroscopy to explore in vivo toxic effects on single living Chlorococcum sp. cells. The study provided important information to achieve a better understanding of the toxic mechanism of triclosan and carbamazepine on living algae Chlorococcum sp.. Triclosan and carbamazepine had distinctive toxic effects on unicellular living algae. Most strikingly, triclosan had more dramatic toxic effects on biochemical components than carbamazepine. Triclosan can affect algae primarily by inhibiting fatty acid synthesis and causing protein aggregation. The toxicity response was irreversible at higher concentration (100.000 μM), but attenuated at lower concentration (0.391 μM) as time extended. Carbamazepine can produce hydrophobic interactions to affect the phospholipid bilayer and work on specific proteins to disfunction the cell membrane. Carbamazepine-exposed cells developed a resistance while extending exposure time. This is the first demonstration from an ecological standpoint that SR-FTIR can provide an innovative approach to reveal the toxicity of emerging pollutants in aquatic environments.
Jia Feng - One of the best experts on this subject based on the ideXlab platform.
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molecular mechanism of lipid accumulation and metabolism of oleaginous Chlorococcum sphacosum gd from soil under salt stress
International Journal of Molecular Sciences, 2021Co-Authors: Jia Feng, Qi Liu, Fangru Nan, Xudong Liu, Shulian XieAbstract:The oleaginous microalgae species Chlorococcum sphacosum GD is a promising feedstock for biodiesel production from soil. However, its metabolic mechanism of lipid production remains unclear. In this study, the lipid accumulation and metabolism mechanisms of Chlorococcum sphacosum GD were analyzed under salt stress based on transcriptome sequencing. The biomass and lipid content of the alga strain were determined under different NaCl concentrations, and total RNA from fresh cells were isolated and sequenced by HiSeq 2000 high throughput sequencing technology. As the salt concentration increased in culture medium, the algal lipid content increased but the biomass decreased. Following transcriptome sequencing by assembly and splicing, 24,128 unigenes were annotated, with read lengths mostly distributed in the 200–300 bp interval. Statistically significant differentially expressed unigenes were observed in different experimental groups, with 2051 up-regulated genes and 1835 down-regulated genes. The lipid metabolism pathway analysis showed that, under salt stress, gene-related fatty acid biosynthesis (ACCase, KASII, KAR, HAD, FATA) was significantly up-regulated, but some gene-related fatty acid degradation was significantly down-regulated. The comprehensive results showed that salt concentration can affect the lipid accumulation and metabolism of C. sphacosum GD, and the lipid accumulation is closely related to the fatty acid synthesis pathway.
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transcriptomic analysis reveals the mechanism on the response of Chlorococcum sp gd to glucose concentration in mixotrophic cultivation
Bioresource Technology, 2019Co-Authors: Fei Zhao, Jia Feng, Qi Liu, Fangru Nan, Xudong Liu, Shulian XieAbstract:Abstract In this study, the performance of Chlorococcum sp. GD in synthetic medium with different glucose concentrations (ranging from 1 to10 g/L) was investigated. Moreover, transcriptome sequencing was conducted to clarify the response of the microalga to glucose concentrations. High concentration of glucose (6–10 g/L) not only did not provide a higher yield of biomass but also inhibited photosynthesis. Transcriptomic analysis revealed that the glucose metabolism mainly depended on the glycolysis and the pentose phosphate pathway (PPP) as the microalga was cultivated with 10 g/L glucose. Meanwhile the tricarboxylic acid (TCA) cycle, oxidative phosphorylation and photosynthesis were significantly inhibited. The significant change on carbon metabolic flux caused by the increase in glucose concentration affected the synthesis of reducing power and ATP, which ultimately influenced the growth of the microalga. Appropriate supplement of organic carbon not only enhances the biomass accumulation but also increases the utilization efficiency of organic carbon.
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the performance of a self flocculating microalga Chlorococcum sp gd in wastewater with different ammonia concentrations
International Journal of Environmental Research and Public Health, 2018Co-Authors: Xuechun Wang, Jia Feng, Qi Liu, Wei Liu, Fangru Nan, Xiaoyan Jiao, Shulian XieAbstract:The performance of a self-flocculating microalga Chlorococcum sp. GD on the flocculation, growth, and lipid accumulation in wastewater with different ammonia nitrogen concentrations was investigated. It was revealed that relative high ammonia nitrogen concentration (20–50 mg·L−1) was beneficial to the flocculation of Chlorococcum sp. GD, and the highest flocculating efficiency was up to 84.4%. It was also found that the highest flocculating efficiency occurred in the middle of the culture (4–5 days) regardless of initial ammonia concentration in wastewater. It was speculated that high flocculating efficiency was likely related to the production of extracellular proteins. 20 mg·L−1 of ammonia was found to be a preferred concentration for both biomass production and lipid accumulation. 92.8% COD, 98.8% ammonia, and 69.4% phosphorus were removed when Chlorococcum sp. GD was cultivated in wastewater with 20 mg·L−1 ammonia. The novelty and significance of the investigation was the integration of flocculation, biomass production, wastewater treatment, and lipid accumulation, simultaneously, which made Chlorococcum sp. GD a potential candidate for wastewater treatment and biodiesel production if harvested in wastewater with suitable ammonia nitrogen concentration.
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effect of sulfate ions on growth and pollutants removal of self flocculating microalga Chlorococcum sp gd in synthetic municipal wastewater
Bioresource Technology, 2017Co-Authors: Junyan Guo, Jia Feng, Qi Liu, Shulian XieAbstract:Abstract Sulfate is a primary sulfur source and can be available in wastewaters. Nevertheless, effect of sulfate ions on growth and pollutants removal of microalgae seems to be less investigated. At the present study, self-flocculating microalga Chlorococcum sp. GD was grown in synthetic municipal wastewater with different sulfate concentrations. Results indicated that Chlorococcum sp. GD grew better in synthetic municipal wastewater with 18, 45, 77, 136 and 271 mg/L SO 4 2− than in wastewater without SO 4 2− . Chlorococcum sp. GD had also excellent removal efficiencies of nitrogen and phosphorus and effectively flocculated in sulfate wastewater. Sulfate deprivation weakened the growth, pollutants removal and self-flocculation of Chlorococcum sp. GD in wastewater. Antioxidative enzymes activity significantly increased and photosynthetic activity significantly decreased when Chlorococcum sp. GD was cultivated in sulfate-free wastewater. Sulfate deprivation probably reduced cell activity of growth, pollutants removal and flocculation via inducing the over-accumulation of reactive oxygen species (ROS).