The Experts below are selected from a list of 849 Experts worldwide ranked by ideXlab platform
S. Aoki - One of the best experts on this subject based on the ideXlab platform.
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Characterization of two genes, Sig1 and Sig2, encoding distinct plastid sigma factors(1) in the moss Physcomitrella patens: phylogenetic relationships to plastid sigma factors in higher plants.
FEBS letters, 2001Co-Authors: K. Hara, M Sugita, R. Takahashi, S. Kato, M Morita, S. AokiAbstract:We isolated the cDNA for a sigma factor from the moss Physcomitrella patens, which possesses unusually large N-terminal extension and the conserved subdomains 1.2-4.2. Phylogenetic analyses indicated that this novel sigma factor and PpSIG1*(2), a plastid sigma factor previously identified from Physcomitrella, were classified into SigA and SigB groups, two major classes of higher plant plastid sigma factors, respectively. According to the nomenclature recently proposed, we renamed PpSIG1* into PpSIG2, and named the novel sigma factor PpSIG1. A transient expression assay using a green fluorescent protein showed that the N-terminal region of PpSIG1 acts as a Chloroplast-Targeting Signal. Reverse transcription-PCR experiments showed that light induces the expression of the Sig1 and Sig2 genes encoding PpSIG1 and PpSIG2, respectively. Thus, PpSIG1 and PpSIG2 are likely plastid sigma factors regulating plastid gene expression in response to light Signals.
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Characterization of two genes, Sig1 and Sig2, encoding distinct plastid σ factors1 in the moss Physcomitrella patens: phylogenetic relationships to plastid σ factors in higher plants
FEBS Letters, 2001Co-Authors: K. Hara, M Sugita, R. Takahashi, S. Kato, M Morita, S. AokiAbstract:We isolated the cDNA for a σ factor from the moss Physcomitrella patens, which possesses unusually large N-terminal extension and the conserved subdomains 1.2–4.2. Phylogenetic analyses indicated that this novel σ factor and PpSIG1* 2, a plastid σ factor previously identified from Physcomitrella, were classified into SigA and SigB groups, two major classes of higher plant plastid σ factors, respectively. According to the nomenclature recently proposed, we renamed PpSIG1* into PpSIG2, and named the novel σ factor PpSIG1. A transient expression assay using a green fluorescent protein showed that the N-terminal region of PpSIG1 acts as a Chloroplast-Targeting Signal. Reverse transcription-PCR experiments showed that light induces the expression of the Sig1 and Sig2 genes encoding PpSIG1 and PpSIG2, respectively. Thus, PpSIG1 and PpSIG2 are likely plastid σ factors regulating plastid gene expression in response to light Signals.
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Cloning and characterization of the cDNA for a plastid σ factor from the moss Physcomitrella patens
Biochimica et biophysica acta, 2001Co-Authors: K. Hara, M Sugita, S. AokiAbstract:Abstract We isolated a cDNA PpSig1 encoding a plastid σ factor from the moss Physcomitrella patens . The PpSIG1 protein is composed of the conserved subdomains for recognition of −10 and −35 promoter elements, core complex binding and DNA melting. Southern blot analysis showed that the moss sig1 gene is likely a member of a small gene family. Transient expression assay using green fluorescent protein demonstrated that the N-terminal region of PpSIG1 functions as a Chloroplast-Targeting Signal peptide. These observations suggest that multiple nuclear-encoded σ factors regulate Chloroplast gene expression in P. patens .
K. Hara - One of the best experts on this subject based on the ideXlab platform.
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Characterization of two genes, Sig1 and Sig2, encoding distinct plastid sigma factors(1) in the moss Physcomitrella patens: phylogenetic relationships to plastid sigma factors in higher plants.
FEBS letters, 2001Co-Authors: K. Hara, M Sugita, R. Takahashi, S. Kato, M Morita, S. AokiAbstract:We isolated the cDNA for a sigma factor from the moss Physcomitrella patens, which possesses unusually large N-terminal extension and the conserved subdomains 1.2-4.2. Phylogenetic analyses indicated that this novel sigma factor and PpSIG1*(2), a plastid sigma factor previously identified from Physcomitrella, were classified into SigA and SigB groups, two major classes of higher plant plastid sigma factors, respectively. According to the nomenclature recently proposed, we renamed PpSIG1* into PpSIG2, and named the novel sigma factor PpSIG1. A transient expression assay using a green fluorescent protein showed that the N-terminal region of PpSIG1 acts as a Chloroplast-Targeting Signal. Reverse transcription-PCR experiments showed that light induces the expression of the Sig1 and Sig2 genes encoding PpSIG1 and PpSIG2, respectively. Thus, PpSIG1 and PpSIG2 are likely plastid sigma factors regulating plastid gene expression in response to light Signals.
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Characterization of two genes, Sig1 and Sig2, encoding distinct plastid σ factors1 in the moss Physcomitrella patens: phylogenetic relationships to plastid σ factors in higher plants
FEBS Letters, 2001Co-Authors: K. Hara, M Sugita, R. Takahashi, S. Kato, M Morita, S. AokiAbstract:We isolated the cDNA for a σ factor from the moss Physcomitrella patens, which possesses unusually large N-terminal extension and the conserved subdomains 1.2–4.2. Phylogenetic analyses indicated that this novel σ factor and PpSIG1* 2, a plastid σ factor previously identified from Physcomitrella, were classified into SigA and SigB groups, two major classes of higher plant plastid σ factors, respectively. According to the nomenclature recently proposed, we renamed PpSIG1* into PpSIG2, and named the novel σ factor PpSIG1. A transient expression assay using a green fluorescent protein showed that the N-terminal region of PpSIG1 acts as a Chloroplast-Targeting Signal. Reverse transcription-PCR experiments showed that light induces the expression of the Sig1 and Sig2 genes encoding PpSIG1 and PpSIG2, respectively. Thus, PpSIG1 and PpSIG2 are likely plastid σ factors regulating plastid gene expression in response to light Signals.
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Cloning and characterization of the cDNA for a plastid σ factor from the moss Physcomitrella patens
Biochimica et biophysica acta, 2001Co-Authors: K. Hara, M Sugita, S. AokiAbstract:Abstract We isolated a cDNA PpSig1 encoding a plastid σ factor from the moss Physcomitrella patens . The PpSIG1 protein is composed of the conserved subdomains for recognition of −10 and −35 promoter elements, core complex binding and DNA melting. Southern blot analysis showed that the moss sig1 gene is likely a member of a small gene family. Transient expression assay using green fluorescent protein demonstrated that the N-terminal region of PpSIG1 functions as a Chloroplast-Targeting Signal peptide. These observations suggest that multiple nuclear-encoded σ factors regulate Chloroplast gene expression in P. patens .
M Sugita - One of the best experts on this subject based on the ideXlab platform.
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Characterization of two genes, Sig1 and Sig2, encoding distinct plastid sigma factors(1) in the moss Physcomitrella patens: phylogenetic relationships to plastid sigma factors in higher plants.
FEBS letters, 2001Co-Authors: K. Hara, M Sugita, R. Takahashi, S. Kato, M Morita, S. AokiAbstract:We isolated the cDNA for a sigma factor from the moss Physcomitrella patens, which possesses unusually large N-terminal extension and the conserved subdomains 1.2-4.2. Phylogenetic analyses indicated that this novel sigma factor and PpSIG1*(2), a plastid sigma factor previously identified from Physcomitrella, were classified into SigA and SigB groups, two major classes of higher plant plastid sigma factors, respectively. According to the nomenclature recently proposed, we renamed PpSIG1* into PpSIG2, and named the novel sigma factor PpSIG1. A transient expression assay using a green fluorescent protein showed that the N-terminal region of PpSIG1 acts as a Chloroplast-Targeting Signal. Reverse transcription-PCR experiments showed that light induces the expression of the Sig1 and Sig2 genes encoding PpSIG1 and PpSIG2, respectively. Thus, PpSIG1 and PpSIG2 are likely plastid sigma factors regulating plastid gene expression in response to light Signals.
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Characterization of two genes, Sig1 and Sig2, encoding distinct plastid σ factors1 in the moss Physcomitrella patens: phylogenetic relationships to plastid σ factors in higher plants
FEBS Letters, 2001Co-Authors: K. Hara, M Sugita, R. Takahashi, S. Kato, M Morita, S. AokiAbstract:We isolated the cDNA for a σ factor from the moss Physcomitrella patens, which possesses unusually large N-terminal extension and the conserved subdomains 1.2–4.2. Phylogenetic analyses indicated that this novel σ factor and PpSIG1* 2, a plastid σ factor previously identified from Physcomitrella, were classified into SigA and SigB groups, two major classes of higher plant plastid σ factors, respectively. According to the nomenclature recently proposed, we renamed PpSIG1* into PpSIG2, and named the novel σ factor PpSIG1. A transient expression assay using a green fluorescent protein showed that the N-terminal region of PpSIG1 acts as a Chloroplast-Targeting Signal. Reverse transcription-PCR experiments showed that light induces the expression of the Sig1 and Sig2 genes encoding PpSIG1 and PpSIG2, respectively. Thus, PpSIG1 and PpSIG2 are likely plastid σ factors regulating plastid gene expression in response to light Signals.
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Cloning and characterization of the cDNA for a plastid σ factor from the moss Physcomitrella patens
Biochimica et biophysica acta, 2001Co-Authors: K. Hara, M Sugita, S. AokiAbstract:Abstract We isolated a cDNA PpSig1 encoding a plastid σ factor from the moss Physcomitrella patens . The PpSIG1 protein is composed of the conserved subdomains for recognition of −10 and −35 promoter elements, core complex binding and DNA melting. Southern blot analysis showed that the moss sig1 gene is likely a member of a small gene family. Transient expression assay using green fluorescent protein demonstrated that the N-terminal region of PpSIG1 functions as a Chloroplast-Targeting Signal peptide. These observations suggest that multiple nuclear-encoded σ factors regulate Chloroplast gene expression in P. patens .
Lih-jen Chen - One of the best experts on this subject based on the ideXlab platform.
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A Novel Chloroplastic Outer Membrane-Targeting Signal That Functions at Both Termini of Passenger Polypeptides
Journal of Biological Chemistry, 1997Co-Authors: Hsou-min Li, Lih-jen ChenAbstract:Abstract Several components in the machinery mediating the import of nuclear-encoded Chloroplastic precursor proteins have been identified. One of the components, OEP34, is an outer membrane protein and is synthesized at its mature size in the cytosol without a distinguishable Chloroplast-Targeting Signal. To address the question of how components in the transport machinery are imported to Chloroplasts themselves, we first identified the Chloroplastic outer membrane-Targeting Signal of OEP34. Using an Arabidopsishomologue of the originally isolated pea OEP34, we show that the outer membrane-Targeting Signal of OEP34 is located within a 10-amino acid hydrophobic core of the C-terminal membrane anchor. Interestingly, this Signal can target a passenger protein to the Chloroplastic outer membrane no matter whether it is placed at the N or C terminus of a passenger protein. Proper insertion of fusion proteins into the outer membrane requires in addition the C-terminal hydrophilic region following the hydrophobic core. Furthermore, passenger proteins fused to the C terminus of the Targeting/insertion Signal were most likely imported into the intermembrane space of the envelope.
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A Novel Chloroplastic Outer Membrane-Targeting Signal That Functions at Both Termini of Passenger Polypeptides
Journal of Biological Chemistry, 1997Co-Authors: Hsou-min Li, Lih-jen ChenAbstract:Abstract Several components in the machinery mediating the import of nuclear-encoded Chloroplastic precursor proteins have been identified. One of the components, OEP34, is an outer membrane protein and is synthesized at its mature size in the cytosol without a distinguishable Chloroplast-Targeting Signal. To address the question of how components in the transport machinery are imported to Chloroplasts themselves, we first identified the Chloroplastic outer membrane-Targeting Signal of OEP34. Using an Arabidopsishomologue of the originally isolated pea OEP34, we show that the outer membrane-Targeting Signal of OEP34 is located within a 10-amino acid hydrophobic core of the C-terminal membrane anchor. Interestingly, this Signal can target a passenger protein to the Chloroplastic outer membrane no matter whether it is placed at the N or C terminus of a passenger protein. Proper insertion of fusion proteins into the outer membrane requires in addition the C-terminal hydrophilic region following the hydrophobic core. Furthermore, passenger proteins fused to the C terminus of the Targeting/insertion Signal were most likely imported into the intermembrane space of the envelope.
M Morita - One of the best experts on this subject based on the ideXlab platform.
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Characterization of two genes, Sig1 and Sig2, encoding distinct plastid sigma factors(1) in the moss Physcomitrella patens: phylogenetic relationships to plastid sigma factors in higher plants.
FEBS letters, 2001Co-Authors: K. Hara, M Sugita, R. Takahashi, S. Kato, M Morita, S. AokiAbstract:We isolated the cDNA for a sigma factor from the moss Physcomitrella patens, which possesses unusually large N-terminal extension and the conserved subdomains 1.2-4.2. Phylogenetic analyses indicated that this novel sigma factor and PpSIG1*(2), a plastid sigma factor previously identified from Physcomitrella, were classified into SigA and SigB groups, two major classes of higher plant plastid sigma factors, respectively. According to the nomenclature recently proposed, we renamed PpSIG1* into PpSIG2, and named the novel sigma factor PpSIG1. A transient expression assay using a green fluorescent protein showed that the N-terminal region of PpSIG1 acts as a Chloroplast-Targeting Signal. Reverse transcription-PCR experiments showed that light induces the expression of the Sig1 and Sig2 genes encoding PpSIG1 and PpSIG2, respectively. Thus, PpSIG1 and PpSIG2 are likely plastid sigma factors regulating plastid gene expression in response to light Signals.
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Characterization of two genes, Sig1 and Sig2, encoding distinct plastid σ factors1 in the moss Physcomitrella patens: phylogenetic relationships to plastid σ factors in higher plants
FEBS Letters, 2001Co-Authors: K. Hara, M Sugita, R. Takahashi, S. Kato, M Morita, S. AokiAbstract:We isolated the cDNA for a σ factor from the moss Physcomitrella patens, which possesses unusually large N-terminal extension and the conserved subdomains 1.2–4.2. Phylogenetic analyses indicated that this novel σ factor and PpSIG1* 2, a plastid σ factor previously identified from Physcomitrella, were classified into SigA and SigB groups, two major classes of higher plant plastid σ factors, respectively. According to the nomenclature recently proposed, we renamed PpSIG1* into PpSIG2, and named the novel σ factor PpSIG1. A transient expression assay using a green fluorescent protein showed that the N-terminal region of PpSIG1 acts as a Chloroplast-Targeting Signal. Reverse transcription-PCR experiments showed that light induces the expression of the Sig1 and Sig2 genes encoding PpSIG1 and PpSIG2, respectively. Thus, PpSIG1 and PpSIG2 are likely plastid σ factors regulating plastid gene expression in response to light Signals.