The Experts below are selected from a list of 1806 Experts worldwide ranked by ideXlab platform
Joanne R Dillon - One of the best experts on this subject based on the ideXlab platform.
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suitability of a Chocolate Agar based medium for the transportation and long term storage of neisseria gonorrhoeae isolates at room temperature
Journal of Microbiological Methods, 2018Co-Authors: S D Thakur, Joanne R DillonAbstract:Neisseria gonorrhoeae isolates were grown on Chocolate Agar slants comprising GC Agar Medium Base supplemented with 1% modified Kellogg's supplement and 1% beef blood hemoglobin. The bacterial growth was covered with sterilized paraffin oil. Cultures were used for transportation and storage of Neisseria gonorrhoeae for 3months at room temperature.
S D Thakur - One of the best experts on this subject based on the ideXlab platform.
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suitability of a Chocolate Agar based medium for the transportation and long term storage of neisseria gonorrhoeae isolates at room temperature
Journal of Microbiological Methods, 2018Co-Authors: S D Thakur, Joanne R DillonAbstract:Neisseria gonorrhoeae isolates were grown on Chocolate Agar slants comprising GC Agar Medium Base supplemented with 1% modified Kellogg's supplement and 1% beef blood hemoglobin. The bacterial growth was covered with sterilized paraffin oil. Cultures were used for transportation and storage of Neisseria gonorrhoeae for 3months at room temperature.
Emilie Bessede - One of the best experts on this subject based on the ideXlab platform.
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maldi tof mass spectrometry for early identification of bacteria grown in blood culture bottles
Journal of Microbiological Methods, 2015Co-Authors: Jeanbenoit Zabbe, Laura Zanardo, Francis Megraud, Emilie BessedeAbstract:This note reports an interesting way to rapidly identify bacteria grown from blood culture bottles. Chocolate Agar plates were inoculated with 1 drop of the positive blood bottle medium. After a 3-hour incubation, the growth veil was submitted to MALDI-TOF mass spectrometry: 77% of the bacteria present have been correctly identified.
Anu Patarisampo - One of the best experts on this subject based on the ideXlab platform.
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identification of urinary tract pathogens after 3 hours urine culture by maldi tof mass spectrometry
Journal of Microbiological Methods, 2016Co-Authors: Johanna Haiko, Risto Hilla, Laura E Savolainen, Anu PatarisampoAbstract:Abstract Complicated urinary tract infections, such as pyelonephritis, may lead to sepsis. Rapid diagnosis is needed to identify the causative urinary pathogen and to verify the appropriate empirical antimicrobial therapy. We describe here a rapid identification method for urinary pathogens: urine is incubated on Chocolate Agar for 3 h at 35 °C with 5% CO 2 and subjected to MALDI-TOF MS analysis by VITEK MS. Overall 207 screened clinical urine samples were tested in parallel with conventional urine culture. The method, called U-si-MALDI-TOF ( u rine s hort i ncubation MALDI-TOF), showed correct identification for 86% of Gram-negative urinary tract pathogens ( Escherichia coli , Klebsiella pneumoniae , and other Enterobacteriaceae), when present at > 10 5 cfu/ml in culture ( n = 107), compared with conventional culture method. However, Gram-positive bacteria ( n = 28) were not successfully identified by U-si-MALDI-TOF. This method is especially suitable for rapid identification of E. coli , the most common cause of urinary tract infections and urosepsis. Turnaround time for identification using U-si-MALDI-TOF compared with conventional urine culture was improved from 24 h to 4–6 h.
Tanja Schulincasonato - One of the best experts on this subject based on the ideXlab platform.
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in vitro susceptibility of bartonella species to 17 antimicrobial compounds comparison of etest and Agar dilution
Journal of Antimicrobial Chemotherapy, 2006Co-Authors: Christina Dorbecker, Anna Sander, Karin Oberle, Tanja SchulincasonatoAbstract:OBJECTIVES: In vitro susceptibility testing of 31 Bartonella spp. strains including 21 Bartonella henselae isolates was performed for 17 antimicrobial agents (telithromycin, four macrolides, five fluoroquinolones, five aminoglycosides, doxycycline and rifampicin). METHODS: MICs were determined by Agar dilution and Etest using Chocolate Agar containing 5% defibrinated sheep blood as assay medium. Longer incubation periods of 3-5 days in a humid atmosphere with 5% CO(2) were required until bacterial growth became visible and MICs could be read. RESULTS: The ketolide telithromycin was the most active agent exhibiting the lowest MICs. The Bartonella spp. were also highly susceptible to macrolides, particularly clarithromycin, and to doxycycline and rifampicin, with MICs of