The Experts below are selected from a list of 114753 Experts worldwide ranked by ideXlab platform
Jinghao Zheng - One of the best experts on this subject based on the ideXlab platform.
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electrospun gelatin polycaprolactone nanofibrous membranes combined with a coculture of bone marrow stromal cells and Chondrocytes for cartilage engineering
International Journal of Nanomedicine, 2015Co-Authors: Xiaomin He, Chuanpei Huang, Bei Feng, Renjie Hu, Zhiwei Xu, Yang Ge, Wei Wang, Hao Wang, Wei Fu, Jinghao ZhengAbstract:Electrospinning has recently received considerable attention, showing notable potential as a novel method of scaffold fabrication for cartilage engineering. The aim of this study was to use a coculture strategy of Chondrocytes combined with electrospun gelatin/polycaprolactone (GT/PCL) membranes, instead of pure Chondrocytes, to evaluate the formation of cartilaginous tissue. We prepared the GT/PCL membranes, seeded bone marrow stromal cell (BMSC)/Chondrocyte cocultures (75% BMSCs and 25% Chondrocytes) in a sandwich model in vitro, and then implanted the constructs subcutaneously into nude mice for 12 weeks. Gross observation, histological and immunohistological evaluation, glycosaminoglycan analyses, Young’s modulus measurement, and immunofluorescence staining were performed postimplantation. We found that the coculture group formed mature cartilage-like tissue, with no statistically significant difference from the Chondrocyte group, and labeled BMSCs could differentiate into Chondrocyte-like cells under the chondrogenic niche of Chondrocytes. This entire strategy indicates that GT/PCL membranes are also a suitable scaffold for stem cell-based cartilage engineering and may provide a potentially clinically feasible approach for cartilage repairs.
Qian Chen - One of the best experts on this subject based on the ideXlab platform.
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subcellular relocation of histone deacetylase 4 regulates growth plate Chondrocyte differentiation through ca2 calmodulin dependent kinase iv
American Journal of Physiology-cell Physiology, 2012Co-Authors: Yingjie Guan, Paul Haines, Richard M. Terek, Qian Chen, Xu Yang, Tingcun ZhaoAbstract:Regulatory mechanisms of Chondrocyte differentiation in the growth plate are incompletely understood. Here, we find that histone deacetylase 4 (HDAC4) is located in the nucleus of Chondrocytes in the proliferation zone and relocates to the cytoplasm of Chondrocytes in the prehypertrophic zone in vivo. This suggests that the relocation of HDAC4 from the nucleus to the cytoplasm may play a role during Chondrocyte differentiation. Expression of active CaMKIV in Chondrocytes promotes HDAC4 relocation into cytoplasm in primary Chondrocytes. Conversely, HDAC4 relocation is blocked by a Ca2+/calmodulin-dependent kinase IV (CaMKIV) inhibitor. This indicates that CaMKIV signaling plays an important role in regulating HDAC4 relocation. In addition, CaMKIV is required for HDAC4 phosphorylation, which is required for HDAC4 association with the cytoplasmic protein 14-3-3. Active CaMKIV also stimulates runt-related transcription factor-2 (RunX2) and type X collagen (Col X) promoter activities and overcomes repression of these promoter activities by HDAC4. Furthermore, CaMKIV increases gene expression of the Chondrocyte differentiation markers Ihh and Col X. Our results demonstrate that CaMKIV induces Chondrocyte differentiation through regulation of HDAC4 subcellular relocation, from the nucleus to the cytoplasm, which results in increased activity of RunX2 and transition of Chondrocytes from the proliferative to the prehypertrophic stage. Thus, CaMKIV plays an important regulatory role during Chondrocyte differentiation.
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Subcellular relocation of histone deacetylase 4 regulates growth plate Chondrocyte differentiation through Ca2+/calmodulin-dependent kinase IV
American Journal of Physiology-cell Physiology, 2012Co-Authors: Yingjie Guan, Paul Haines, Richard M. Terek, Qian Chen, Xu Yang, Tingcun ZhaoAbstract:Regulatory mechanisms of Chondrocyte differentiation in the growth plate are incompletely understood. Here, we find that histone deacetylase 4 (HDAC4) is located in the nucleus of Chondrocytes in the proliferation zone and relocates to the cytoplasm of Chondrocytes in the prehypertrophic zone in vivo. This suggests that the relocation of HDAC4 from the nucleus to the cytoplasm may play a role during Chondrocyte differentiation. Expression of active CaMKIV in Chondrocytes promotes HDAC4 relocation into cytoplasm in primary Chondrocytes. Conversely, HDAC4 relocation is blocked by a Ca2+/calmodulin-dependent kinase IV (CaMKIV) inhibitor. This indicates that CaMKIV signaling plays an important role in regulating HDAC4 relocation. In addition, CaMKIV is required for HDAC4 phosphorylation, which is required for HDAC4 association with the cytoplasmic protein 14-3-3. Active CaMKIV also stimulates runt-related transcription factor-2 (RunX2) and type X collagen (Col X) promoter activities and overcomes repression of these promoter activities by HDAC4. Furthermore, CaMKIV increases gene expression of the Chondrocyte differentiation markers Ihh and Col X. Our results demonstrate that CaMKIV induces Chondrocyte differentiation through regulation of HDAC4 subcellular relocation, from the nucleus to the cytoplasm, which results in increased activity of RunX2 and transition of Chondrocytes from the proliferative to the prehypertrophic stage. Thus, CaMKIV plays an important regulatory role during Chondrocyte differentiation.
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mechanoregulation of Chondrocyte proliferation maturation and hypertrophy ion channel dependent transduction of matrix deformation signals
Experimental Cell Research, 2000Co-Authors: Qiuqian Wu, Qian ChenAbstract:Abstract Mechanical stress-induced matrix deformation plays a fundamental role in regulating cellular activities; however, little is known about its underlying mechanisms. To understand the effects of matrix deformation on Chondrocytes, we characterized primary Chondrocytes cultured on three-dimensional collagen scaffoldings, which can be loaded mechanically with a computer-controlled “Bio-Stretch” device. Cyclic matrix deformation greatly stimulated proliferation of immature Chondrocytes, but not that of hypertrophic Chondrocytes. This indicates that mechanical stimulation of Chondrocyte proliferation is developmental stage specific. Synthesis of cartilage matrix protein (CMP/matrilin-1), a mature Chondrocyte marker, and type X collagen, a hypertrophic Chondrocyte marker, was up-regulated by stretch-induced matrix deformation. Therefore, genes of CMP and type X collagen are responsive to mechanical stress. Mechanical stimulation of the mRNA levels of CMP and type X collagen occurred exactly at the same time points when these markers were synthesized by nonloading cells. This indicates that cyclic matrix deformation does not alter the speed of differentiation, but affects the extent of differentiation. The addition of the stretch-activated channel blocker gadolinium during loading abolished mechanical stimulation of Chondrocyte proliferation, but did not affect the up-regulation of CMP mRNA by mechanical stretch. In contrast, the calcium channel blocker nifedipine inhibited both the stretch-induced proliferation and the increase of CMP mRNA. This suggests that stretch-induced matrix deformation regulates Chondrocyte proliferation and differentiation via two signal transduction pathways, with stretch-activated channels involved in transducing the proliferative signals and calcium channels involved in transducing the signals for both proliferation and differentiation.
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progression and recapitulation of the Chondrocyte differentiation program cartilage matrix protein is a marker for cartilage maturation
Developmental Biology, 1995Co-Authors: Qian Chen, David M Johnson, Dominik R Haudenschild, Paul F GoetinckAbstract:Abstract During endochondral bone formation, Chondrocytes in the cartilaginous anlage of long bones progress through a spatially and temporally regulated differentiation program before being replaced by bone. To understand this process, we have characterized the differentiation program and analyzed the relationship between Chondrocytes and their extracellular environment in the regulation of the program. Our results indicate that, within an epiphyseal growth plate, the zone of proliferating Chondrocytes is not contiguous with the zone of hypertrophic Chondrocytes identified by the transcription of the type X collagen gene. We find that the postproliferative Chondrocytes which make up the zone between the zones of proliferation and hypertrophy specifically transcribe the gene for cartilage matrix protein (CMP). This zone has been termed the zone of maturation. The identification of this unique population of Chondrocytes demonstrates that the Chondrocyte differentiation program consists of at least three stages. CMP translation products are present in the matrix surrounding the nonproliferative Chondrocytes of both the zones of maturation and hypertrophy. Thus, CMP is a marker for postmitotic Chondrocytes. As a result of the changes in gene expression during the differentiation program, Chondrocytes in each zone reside in an extracellular matrix with a unique macromolecular composition. Chondrocytes in primary cell culture can proceed through the same differentiation program as they do in the cartilaginous rudiments. In culture, a wave of differentiation begins in the center of a colony and spreads to its periphery. The cessation of proliferation coincides with the appearance of CMP and eventually the cells undergo hypertrophy and synthesize type X collagen. These results reveal distinct switches at the proliferative–maturation transition and at the maturation–hypertrophy transition during Chondrocyte differentiation and indicate that Chondrocytes synthesize new matrix molecules and thus modify their preexisting microenvironment as differentiation progresses. However, when “terminally” differentiated hypertrophic Chondrocytes are released from their surrounding environment and incubated in pellet culture, they stop type X collagen synthesis, resume proliferation, and reinitiate aggrecan synthesis. Eventually they cease proliferation and reinitiate CMP synthesis and finally type X collagen. Thus they are capable of recapitulating all three stages of the differentiation programin vitro.The data suggest a high degree of plasticity in the Chondrocyte differentiation program and demonstrate that the progression and maintenance of this program is regulated, at least in part, by the extracellular environment which surrounds a differentiating Chondrocyte during endochondral bone formation.
Xiaomin He - One of the best experts on this subject based on the ideXlab platform.
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Electrospun gelatin/polycaprolactone nanofibrous membranes combined with a coculture of bone marrow stromal cells and Chondrocytes for cartilage engineering.
International Journal of Nanomedicine, 2015Co-Authors: Xiaomin He, Chuanpei Huang, Bei Feng, Renjie Hu, Zhiwei Xu, Yang Ge, Wei Wang, Hao Wang, Wei FuAbstract:Electrospinning has recently received considerable attention, showing notable potential as a novel method of scaffold fabrication for cartilage engineering. The aim of this study was to use a coculture strategy of Chondrocytes combined with electrospun gelatin/polycaprolactone (GT/PCL) membranes, instead of pure Chondrocytes, to evaluate the formation of cartilaginous tissue. We prepared the GT/PCL membranes, seeded bone marrow stromal cell (BMSC)/Chondrocyte cocultures (75% BMSCs and 25% Chondrocytes) in a sandwich model in vitro, and then implanted the constructs subcutaneously into nude mice for 12 weeks. Gross observation, histological and immunohistological evaluation, glycosaminoglycan analyses, Young’s modulus measurement, and immunofluorescence staining were performed postimplantation. We found that the coculture group formed mature cartilage-like tissue, with no statistically significant difference from the Chondrocyte group, and labeled BMSCs could differentiate into Chondrocyte-like cells under the chondrogenic niche of Chondrocytes. This entire strategy indicates that GT/PCL membranes are also a suitable scaffold for stem cell-based cartilage engineering and may provide a potentially clinically feasible approach for cartilage repairs.
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electrospun gelatin polycaprolactone nanofibrous membranes combined with a coculture of bone marrow stromal cells and Chondrocytes for cartilage engineering
International Journal of Nanomedicine, 2015Co-Authors: Xiaomin He, Chuanpei Huang, Bei Feng, Renjie Hu, Zhiwei Xu, Yang Ge, Wei Wang, Hao Wang, Wei Fu, Jinghao ZhengAbstract:Electrospinning has recently received considerable attention, showing notable potential as a novel method of scaffold fabrication for cartilage engineering. The aim of this study was to use a coculture strategy of Chondrocytes combined with electrospun gelatin/polycaprolactone (GT/PCL) membranes, instead of pure Chondrocytes, to evaluate the formation of cartilaginous tissue. We prepared the GT/PCL membranes, seeded bone marrow stromal cell (BMSC)/Chondrocyte cocultures (75% BMSCs and 25% Chondrocytes) in a sandwich model in vitro, and then implanted the constructs subcutaneously into nude mice for 12 weeks. Gross observation, histological and immunohistological evaluation, glycosaminoglycan analyses, Young’s modulus measurement, and immunofluorescence staining were performed postimplantation. We found that the coculture group formed mature cartilage-like tissue, with no statistically significant difference from the Chondrocyte group, and labeled BMSCs could differentiate into Chondrocyte-like cells under the chondrogenic niche of Chondrocytes. This entire strategy indicates that GT/PCL membranes are also a suitable scaffold for stem cell-based cartilage engineering and may provide a potentially clinically feasible approach for cartilage repairs.
Wei Fu - One of the best experts on this subject based on the ideXlab platform.
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Electrospun gelatin/polycaprolactone nanofibrous membranes combined with a coculture of bone marrow stromal cells and Chondrocytes for cartilage engineering.
International Journal of Nanomedicine, 2015Co-Authors: Xiaomin He, Chuanpei Huang, Bei Feng, Renjie Hu, Zhiwei Xu, Yang Ge, Wei Wang, Hao Wang, Wei FuAbstract:Electrospinning has recently received considerable attention, showing notable potential as a novel method of scaffold fabrication for cartilage engineering. The aim of this study was to use a coculture strategy of Chondrocytes combined with electrospun gelatin/polycaprolactone (GT/PCL) membranes, instead of pure Chondrocytes, to evaluate the formation of cartilaginous tissue. We prepared the GT/PCL membranes, seeded bone marrow stromal cell (BMSC)/Chondrocyte cocultures (75% BMSCs and 25% Chondrocytes) in a sandwich model in vitro, and then implanted the constructs subcutaneously into nude mice for 12 weeks. Gross observation, histological and immunohistological evaluation, glycosaminoglycan analyses, Young’s modulus measurement, and immunofluorescence staining were performed postimplantation. We found that the coculture group formed mature cartilage-like tissue, with no statistically significant difference from the Chondrocyte group, and labeled BMSCs could differentiate into Chondrocyte-like cells under the chondrogenic niche of Chondrocytes. This entire strategy indicates that GT/PCL membranes are also a suitable scaffold for stem cell-based cartilage engineering and may provide a potentially clinically feasible approach for cartilage repairs.
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electrospun gelatin polycaprolactone nanofibrous membranes combined with a coculture of bone marrow stromal cells and Chondrocytes for cartilage engineering
International Journal of Nanomedicine, 2015Co-Authors: Xiaomin He, Chuanpei Huang, Bei Feng, Renjie Hu, Zhiwei Xu, Yang Ge, Wei Wang, Hao Wang, Wei Fu, Jinghao ZhengAbstract:Electrospinning has recently received considerable attention, showing notable potential as a novel method of scaffold fabrication for cartilage engineering. The aim of this study was to use a coculture strategy of Chondrocytes combined with electrospun gelatin/polycaprolactone (GT/PCL) membranes, instead of pure Chondrocytes, to evaluate the formation of cartilaginous tissue. We prepared the GT/PCL membranes, seeded bone marrow stromal cell (BMSC)/Chondrocyte cocultures (75% BMSCs and 25% Chondrocytes) in a sandwich model in vitro, and then implanted the constructs subcutaneously into nude mice for 12 weeks. Gross observation, histological and immunohistological evaluation, glycosaminoglycan analyses, Young’s modulus measurement, and immunofluorescence staining were performed postimplantation. We found that the coculture group formed mature cartilage-like tissue, with no statistically significant difference from the Chondrocyte group, and labeled BMSCs could differentiate into Chondrocyte-like cells under the chondrogenic niche of Chondrocytes. This entire strategy indicates that GT/PCL membranes are also a suitable scaffold for stem cell-based cartilage engineering and may provide a potentially clinically feasible approach for cartilage repairs.
Bei Feng - One of the best experts on this subject based on the ideXlab platform.
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Electrospun gelatin/polycaprolactone nanofibrous membranes combined with a coculture of bone marrow stromal cells and Chondrocytes for cartilage engineering.
International Journal of Nanomedicine, 2015Co-Authors: Xiaomin He, Chuanpei Huang, Bei Feng, Renjie Hu, Zhiwei Xu, Yang Ge, Wei Wang, Hao Wang, Wei FuAbstract:Electrospinning has recently received considerable attention, showing notable potential as a novel method of scaffold fabrication for cartilage engineering. The aim of this study was to use a coculture strategy of Chondrocytes combined with electrospun gelatin/polycaprolactone (GT/PCL) membranes, instead of pure Chondrocytes, to evaluate the formation of cartilaginous tissue. We prepared the GT/PCL membranes, seeded bone marrow stromal cell (BMSC)/Chondrocyte cocultures (75% BMSCs and 25% Chondrocytes) in a sandwich model in vitro, and then implanted the constructs subcutaneously into nude mice for 12 weeks. Gross observation, histological and immunohistological evaluation, glycosaminoglycan analyses, Young’s modulus measurement, and immunofluorescence staining were performed postimplantation. We found that the coculture group formed mature cartilage-like tissue, with no statistically significant difference from the Chondrocyte group, and labeled BMSCs could differentiate into Chondrocyte-like cells under the chondrogenic niche of Chondrocytes. This entire strategy indicates that GT/PCL membranes are also a suitable scaffold for stem cell-based cartilage engineering and may provide a potentially clinically feasible approach for cartilage repairs.
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electrospun gelatin polycaprolactone nanofibrous membranes combined with a coculture of bone marrow stromal cells and Chondrocytes for cartilage engineering
International Journal of Nanomedicine, 2015Co-Authors: Xiaomin He, Chuanpei Huang, Bei Feng, Renjie Hu, Zhiwei Xu, Yang Ge, Wei Wang, Hao Wang, Wei Fu, Jinghao ZhengAbstract:Electrospinning has recently received considerable attention, showing notable potential as a novel method of scaffold fabrication for cartilage engineering. The aim of this study was to use a coculture strategy of Chondrocytes combined with electrospun gelatin/polycaprolactone (GT/PCL) membranes, instead of pure Chondrocytes, to evaluate the formation of cartilaginous tissue. We prepared the GT/PCL membranes, seeded bone marrow stromal cell (BMSC)/Chondrocyte cocultures (75% BMSCs and 25% Chondrocytes) in a sandwich model in vitro, and then implanted the constructs subcutaneously into nude mice for 12 weeks. Gross observation, histological and immunohistological evaluation, glycosaminoglycan analyses, Young’s modulus measurement, and immunofluorescence staining were performed postimplantation. We found that the coculture group formed mature cartilage-like tissue, with no statistically significant difference from the Chondrocyte group, and labeled BMSCs could differentiate into Chondrocyte-like cells under the chondrogenic niche of Chondrocytes. This entire strategy indicates that GT/PCL membranes are also a suitable scaffold for stem cell-based cartilage engineering and may provide a potentially clinically feasible approach for cartilage repairs.