The Experts below are selected from a list of 273 Experts worldwide ranked by ideXlab platform
David C Baulcombe - One of the best experts on this subject based on the ideXlab platform.
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a potato hypersensitive resistance gene against potato virus x maps to a resistance gene cluster on Chromosome 5
Theoretical and Applied Genetics, 1997Co-Authors: W De Jong, A Forsyth, Dario Leister, Christiane Gebhardt, David C BaulcombeAbstract:The dominant Nb gene of potato confers strain-specific hypersensitive resistance against potato virus X (PVX). A population segregating for Nb was screened for resistance by inoculating with PVX strain CP2, which is sensitive to Nb. Through a combination of bulked segregant analysis and selective restriction fragment amplification, several amplified fragment length polymorphism (AFLP) markers linked to Nb were identified. These were cloned and converted into dominant cleaved amplified polymorphic sequence (CAPS) markers. The segregation of these markers in a Lycopersicon esculentum×L. pennellii mapping population suggested that Nb is located on Chromosome 5. This was confirmed by examining resistant and susceptible potato individuals with several tomato and potato Chromosome-5-specific markers. Nb maps to a region of Chromosome 5 where several other resistance genes– including R1, a resistance gene against Phytophthora infestans, Gpa, a locus that confers resistance against Globodera pallida, and Rx2, a gene that confers extreme resistance against PVX–have previously been identified.
W De Jong - One of the best experts on this subject based on the ideXlab platform.
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a potato hypersensitive resistance gene against potato virus x maps to a resistance gene cluster on Chromosome 5
Theoretical and Applied Genetics, 1997Co-Authors: W De Jong, A Forsyth, Dario Leister, Christiane Gebhardt, David C BaulcombeAbstract:The dominant Nb gene of potato confers strain-specific hypersensitive resistance against potato virus X (PVX). A population segregating for Nb was screened for resistance by inoculating with PVX strain CP2, which is sensitive to Nb. Through a combination of bulked segregant analysis and selective restriction fragment amplification, several amplified fragment length polymorphism (AFLP) markers linked to Nb were identified. These were cloned and converted into dominant cleaved amplified polymorphic sequence (CAPS) markers. The segregation of these markers in a Lycopersicon esculentum×L. pennellii mapping population suggested that Nb is located on Chromosome 5. This was confirmed by examining resistant and susceptible potato individuals with several tomato and potato Chromosome-5-specific markers. Nb maps to a region of Chromosome 5 where several other resistance genes– including R1, a resistance gene against Phytophthora infestans, Gpa, a locus that confers resistance against Globodera pallida, and Rx2, a gene that confers extreme resistance against PVX–have previously been identified.
Seiji Kojima - One of the best experts on this subject based on the ideXlab platform.
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Myelodysplastic syndromes in a pediatric patient with Cri du Chat syndrome with a ring Chromosome 5
International Journal of Hematology, 2020Co-Authors: Akifumi Nozawa, Michio Ozeki, Shiho Yasue, Saori Endo, Tomonori Kadowaki, Hidenori Ohnishi, Hideki Muramatsu, Asahito Hama, Yoshiyuki Takahashi, Seiji KojimaAbstract:Few hematological complications have previously been reported in association with Cri du Chat syndrome (CdCS). A case of myelodysplastic syndromes (MDS) in a pediatric patient with CdCS is herein presented. A 17-year-old female with CdCS caused by ring Chromosome 5 was admitted to the hospital for investigation of a 1-month history of anemia. Based on the morphological findings of bone marrow, the patient was diagnosed with refractory cytopenia with multilineage dysplasia. The risk group was classified as intermediate-1 in the International Prognostic Scoring System (IPSS), and low in the revised IPSS. Assessment by microarray comparative genomic hybridization (CGH) identified the breakpoints of ring Chromosome 5 as 46,XX,r(5)(p14.3q35.3). This revealed that the 5q terminal deletion did not include the common deleted region of MDS with del(5q). Treatment with azacitidine was initiated to control disease progression and improve quality of life. At baseline, the patient had a mean transfusion requirement of 3 units/month, which decreased to 2 units/month after six cycles of azacitidine and to 1 unit/month after 10 cycles of azacitidine. Cytopenia observed in the presented case seemed irrelevant to ring Chromosome 5 which is the causative cytogenetic abnormality of CdCS, and further analyses may be needed to clarify the pathogenesis.
C Cieuta - One of the best experts on this subject based on the ideXlab platform.
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No evidence for a susceptibility locus for idiopathic generalized epilepsy on Chromosome 5 in families with typical absence seizures.
Epilepsy research, 2020Co-Authors: C Windemuth, H Schulz, K Saar, E Gennaro, A Bianchi, F Zara, C Bulteau, A Kaminska, D Ville, C CieutaAbstract:A recent genome-wide scan revealed suggestive evidence for two susceptibility loci for idiopathic generalized epilepsy (IGE) in the chromosomal regions 5p15 and 5q14-q22 in families with typical absence seizures. The present replication study tested the validity of the tentative IGE loci on Chromosome 5. Our study included 99 multiplex families in which at least one family member had typical absence seizures. Parametric and non-parametric multipoint linkage analyses were carried out between the IGE trait and 23 microsatellite polymorphisms covering the entire region of Chromosome 5. Multipoint parametric heterogeneity lod scores < -2 were obtained along Chromosome 5 when a proportion of linked families greater than 50% was assumed under recessive inheritance and > 60% under dominant inheritance. Furthermore, non-parametric multipoint linkage analyses revealed no hint of linkage throughout the candidate region (P > 0.05). Accordingly, we failed to support previous evidence for common IGE loci on Chromosome 5. If there is a susceptibility locus for IGE on Chromosome 5 then the size of the effect or the proportion of linked families is too small to detect linkage in the investigated family sample.
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No evidence for a susceptibility locus for idiopathic generalized epilepsy on Chromosome 5 in families with typical absence seizures
Epilepsy Research, 2002Co-Authors: C Windemuth, H Schulz, K Saar, E Gennaro, A Bianchi, F Zara, C Bulteau, A Kaminska, D Ville, C CieutaAbstract:A recent genome-wide scan revealed suggestive evidence for two susceptibility loci for idiopathic generalized epilepsy (IGE) in the chromosomal regions 5p15 and 5q14–q22 in families with typical absence seizures. The present replication study tested the validity of the tentative IGE loci on Chromosome 5. Our study included 99 multiplex families in which at least one family member had typical absence seizures. Parametric and non-parametric multipoint linkage analyses were carried out between the IGE trait and 23 microsatellite polymorphisms covering the entire region of Chromosome 5. Multipoint parametric heterogeneity lod scores 60% under dominant inheritance. Furthermore, non-parametric multipoint linkage analyses revealed no hint of linkage throughout the candidate region (P>0.05). Accordingly, we failed to support previous evidence for common IGE loci on Chromosome 5. If there is a susceptibility locus for IGE on Chromosome 5 then the size of the effect or the proportion of linked families is too small to detect linkage in the investigated family sample.
Christiane Gebhardt - One of the best experts on this subject based on the ideXlab platform.
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a potato hypersensitive resistance gene against potato virus x maps to a resistance gene cluster on Chromosome 5
Theoretical and Applied Genetics, 1997Co-Authors: W De Jong, A Forsyth, Dario Leister, Christiane Gebhardt, David C BaulcombeAbstract:The dominant Nb gene of potato confers strain-specific hypersensitive resistance against potato virus X (PVX). A population segregating for Nb was screened for resistance by inoculating with PVX strain CP2, which is sensitive to Nb. Through a combination of bulked segregant analysis and selective restriction fragment amplification, several amplified fragment length polymorphism (AFLP) markers linked to Nb were identified. These were cloned and converted into dominant cleaved amplified polymorphic sequence (CAPS) markers. The segregation of these markers in a Lycopersicon esculentum×L. pennellii mapping population suggested that Nb is located on Chromosome 5. This was confirmed by examining resistant and susceptible potato individuals with several tomato and potato Chromosome-5-specific markers. Nb maps to a region of Chromosome 5 where several other resistance genes– including R1, a resistance gene against Phytophthora infestans, Gpa, a locus that confers resistance against Globodera pallida, and Rx2, a gene that confers extreme resistance against PVX–have previously been identified.