The Experts below are selected from a list of 270 Experts worldwide ranked by ideXlab platform
Jun Shimazaki - One of the best experts on this subject based on the ideXlab platform.
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suppression of metastasis of rat prostatic cancer by introducing human Chromosome 8
Cancer Research, 1994Co-Authors: Tomohiko Ichikawa, Naoki Nihei, Hiroyoshi Suzuki, Mitsuo Oshimura, Yusuke Nakamura, Isamu Hayata, John T Isaacs, Jun ShimazakiAbstract:In previous allelotype analyses of human prostatic cancer specimens, allelic loss on the short arm of Chromosome 8 is frequently observed. However, it is still unclear whether this allelic loss is an initial event or a later one in development of prostatic cancer. Our previous studies demonstrate that introduction of human Chromosome 11 into highly metastatic rat prostatic cancer cells results in suppression of metastatic ability without suppression of the in vivo growth rate or tumorigenicity of the hybrid cells (T. Ichikawa et al. Cancer Res., 52: 3486–3490, 1992). To clarify the role of human Chromosome 8 in prostatic cancer, this Chromosome was introduced into highly metastatic rat prostatic cancer cells using microcell-mediated Chromosome transfer. Introduction of human Chromosome 8 resulted in suppression of metastatic ability of the microcell hybrids, whereas no suppression of the in vivo growth rate or tumorigenicity was observed. These results demonstrate that human Chromosome 8 contains metastasls suppressor gene(s) for prostatic cancer derived from a rat. These also suggest that human Chromosome 8 has an important role in development of prostatic cancer.
Tomohiko Ichikawa - One of the best experts on this subject based on the ideXlab platform.
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suppression of metastasis of rat prostatic cancer by introducing human Chromosome 8
Cancer Research, 1994Co-Authors: Tomohiko Ichikawa, Naoki Nihei, Hiroyoshi Suzuki, Mitsuo Oshimura, Yusuke Nakamura, Isamu Hayata, John T Isaacs, Jun ShimazakiAbstract:In previous allelotype analyses of human prostatic cancer specimens, allelic loss on the short arm of Chromosome 8 is frequently observed. However, it is still unclear whether this allelic loss is an initial event or a later one in development of prostatic cancer. Our previous studies demonstrate that introduction of human Chromosome 11 into highly metastatic rat prostatic cancer cells results in suppression of metastatic ability without suppression of the in vivo growth rate or tumorigenicity of the hybrid cells (T. Ichikawa et al. Cancer Res., 52: 3486–3490, 1992). To clarify the role of human Chromosome 8 in prostatic cancer, this Chromosome was introduced into highly metastatic rat prostatic cancer cells using microcell-mediated Chromosome transfer. Introduction of human Chromosome 8 resulted in suppression of metastatic ability of the microcell hybrids, whereas no suppression of the in vivo growth rate or tumorigenicity was observed. These results demonstrate that human Chromosome 8 contains metastasls suppressor gene(s) for prostatic cancer derived from a rat. These also suggest that human Chromosome 8 has an important role in development of prostatic cancer.
Edson J Pontes - One of the best experts on this subject based on the ideXlab platform.
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fluorescence in situ hybridization analysis of 8p allelic loss and Chromosome 8 instability in human prostate cancer
Cancer Research, 1994Co-Authors: Jill A Macoska, Tanya M Trybus, Wael Sakr, Margaret C Wolf, Philip D Benson, Isaac J Powell, Edson J PontesAbstract:Abstract Cytogenetic and molecular biological studies have demonstrated deletion of sequences that map to the short arm of Chromosome 8 (8p) in tumors from several organ systems, including sequences that map within or near 8p22 in human prostate tumors. Recent studies in our laboratory have suggested that deletion of sequences on 8p may be concurrent with alterations in dosage for Chromosome 8. In order to further investigate this finding, the present study has applied fluorescence in situ hybridization (FISH) techniques to determine the status of Chromosome 8 in prostate tumors that have undergone deletion of sequences at 8p22. Dosage of 8p22 sequences was assayed utilizing unique sequence cosmid DNA probes by FISH and confirmed by amplification of microsatellite sequences by polymerase chain reaction (PCR). Chromosome 8 dosage was assayed by FISH utilizing both unique sequence cosmid probe DNA (specific to the 8q13.1-q13.3 chromosomal region) and pericentromeric probe DNA. FISH analysis of 10 specimens of normal or benign prostatic hyperplasia tissues paired with 9 tumor and one prostatic intraepithelial neoplasia tissues from the same patients for dosage at 8p, 8cen, and 8q, and PCR analysis for dosage at 8p, demonstrated that ( a ) FISH provided a more precise means of evaluating allelic loss than PCR in prostate tissue; ( b ) 8p22 sequence losses occurred frequently in prostate tumors; ( c ) 8p22 sequence losses were most often detected in the absence of 8cen or 8q sequence dosage alterations, although they were sometimes accompanied by gain or loss of 8cen or 8q sequences; and ( d ) the pattern of 8p22 sequence losses was most often widespread rather than focal. This study is the first to describe FISH analysis of interphase nuclei within tissue sections using cosmid probe DNAs.
John T Isaacs - One of the best experts on this subject based on the ideXlab platform.
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suppression of metastasis of rat prostatic cancer by introducing human Chromosome 8
Cancer Research, 1994Co-Authors: Tomohiko Ichikawa, Naoki Nihei, Hiroyoshi Suzuki, Mitsuo Oshimura, Yusuke Nakamura, Isamu Hayata, John T Isaacs, Jun ShimazakiAbstract:In previous allelotype analyses of human prostatic cancer specimens, allelic loss on the short arm of Chromosome 8 is frequently observed. However, it is still unclear whether this allelic loss is an initial event or a later one in development of prostatic cancer. Our previous studies demonstrate that introduction of human Chromosome 11 into highly metastatic rat prostatic cancer cells results in suppression of metastatic ability without suppression of the in vivo growth rate or tumorigenicity of the hybrid cells (T. Ichikawa et al. Cancer Res., 52: 3486–3490, 1992). To clarify the role of human Chromosome 8 in prostatic cancer, this Chromosome was introduced into highly metastatic rat prostatic cancer cells using microcell-mediated Chromosome transfer. Introduction of human Chromosome 8 resulted in suppression of metastatic ability of the microcell hybrids, whereas no suppression of the in vivo growth rate or tumorigenicity was observed. These results demonstrate that human Chromosome 8 contains metastasls suppressor gene(s) for prostatic cancer derived from a rat. These also suggest that human Chromosome 8 has an important role in development of prostatic cancer.
Isamu Hayata - One of the best experts on this subject based on the ideXlab platform.
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suppression of metastasis of rat prostatic cancer by introducing human Chromosome 8
Cancer Research, 1994Co-Authors: Tomohiko Ichikawa, Naoki Nihei, Hiroyoshi Suzuki, Mitsuo Oshimura, Yusuke Nakamura, Isamu Hayata, John T Isaacs, Jun ShimazakiAbstract:In previous allelotype analyses of human prostatic cancer specimens, allelic loss on the short arm of Chromosome 8 is frequently observed. However, it is still unclear whether this allelic loss is an initial event or a later one in development of prostatic cancer. Our previous studies demonstrate that introduction of human Chromosome 11 into highly metastatic rat prostatic cancer cells results in suppression of metastatic ability without suppression of the in vivo growth rate or tumorigenicity of the hybrid cells (T. Ichikawa et al. Cancer Res., 52: 3486–3490, 1992). To clarify the role of human Chromosome 8 in prostatic cancer, this Chromosome was introduced into highly metastatic rat prostatic cancer cells using microcell-mediated Chromosome transfer. Introduction of human Chromosome 8 resulted in suppression of metastatic ability of the microcell hybrids, whereas no suppression of the in vivo growth rate or tumorigenicity was observed. These results demonstrate that human Chromosome 8 contains metastasls suppressor gene(s) for prostatic cancer derived from a rat. These also suggest that human Chromosome 8 has an important role in development of prostatic cancer.