The Experts below are selected from a list of 44262 Experts worldwide ranked by ideXlab platform
Roohollah Sharifi - One of the best experts on this subject based on the ideXlab platform.
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effects of tomato sauce consumption on apoptotic cell death in Prostate benign hyperplasia and carcinoma
Nutrition and Cancer, 2003Co-Authors: Phyllis E. Bowen, Longwen Chen, Claudine Duncan, Luna Ghosh, Roohollah Sharifi, Konstantin ChristovAbstract:Population studies have suggested that lycopene, which is mostly found in tomato and tomato products, may reduce the risk of Prostate cancer. We previously found that tomato sauce consumption prior to Prostatectomy for Prostate cancer decreased serum Prostate specific antigen, decreased oxidative DNA damage, and increased lycopene concentrations in Prostate Tissue (Chen et al., 2001). Here, we extended those investigations to determine whether apoptotic cell death and associated Bcl-2 and Bax proteins were modulated by tomato sauce intervention. Thirty-two patients diagnosed by biopsy with Prostate carcinoma were given tomato sauce pasta entrees (30 mg lycopene/day) for 3 wk before Prostatectomy. Thirty-four patients with Prostate cancer who did not consume tomato sauce and underwent Prostatectomy served as controls. When tumor areas with the most apoptotic cells were compared in the biopsy (before) and resected Prostate Tissue (after), tomato sauce consumption increased apoptotic cells in benign Prostate...
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liquid chromatography mass spectrometry of cis and all trans lycopene in human serum and Prostate Tissue after dietary supplementation with tomato sauce
Journal of Agricultural and Food Chemistry, 2002Co-Authors: Richard B Van Breemen, Longwen Chen, Claudine Duncan, Maria Stacewiczsapuntzakis, Marlos A G Viana, Xiaoying Xu, Phyllis E. Bowen, Roohollah SharifiAbstract:Several epidemiological studies suggest a lower incidence of Prostate cancer in men who routinely consume tomato products. Tomatoes are the primary dietary source of lycopene, which is among the most potent antioxidants of the carotenoids. Men with clinical stage T1 or T2 Prostate adenocarcinoma were recruited (n = 32) and consumed tomato sauce based pasta dishes for 3 weeks (equivalent to 30 mg of lycopene per day) before radical prostectomy. Prostate Tissue from needle biopsy just before intervention and prostectomy after supplementation from a subset of 11 subjects was evaluated for both total lycopene and lycopene geometrical isomer ratios. A gradient HPLC system using a C(18) column with UV-vis absorbance detection was used to measure total lycopene. Because the absorbance detector was insufficiently sensitive, HPLC with a C(30) column and positive ion atmospheric pressure chemical ionization mass spectrometric (LC-MS) detection was developed as a new assay to measure the ratio of lycopene cis/trans isomers in these samples. The limit of detection of the LC-MS method was determined to be 0.93 pmol of lycopene on-column, and a linear response was obtained over 3 orders of magnitude. Total lycopene in serum increased 2.0-fold from 35.6 to 69.9 microg/dL (from 0.664 to 1.30 microM) as a result of dietary supplementation with tomato sauce, whereas total lycopene in Prostate Tissue increased 3.0-fold from 0.196 to 0.582 ng/mg of Tissue (from 0.365 to 1.09 pmol/mg). all-trans-Lycopene and at least 14 cis-isomer peaks were detected in Prostate Tissue and serum. The mean proportion of all-trans-lycopene in Prostate Tissue was approximately 12.4% of total lycopene before supplementation but increased to 22.7% after dietary intervention with tomato sauce. In serum there was only a 2.8% but statistically significant increase in the proportion of all-trans-lycopene after intervention. These results indicate that short-term supplementation with tomato sauce containing primarily all-trans-lycopene (83% of total lycopene) results in substantial increases in total lycopene in serum and Prostate and a substantial increase in all-trans-lycopene in Prostate but relatively less in serum.
Phyllis E. Bowen - One of the best experts on this subject based on the ideXlab platform.
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effects of tomato sauce consumption on apoptotic cell death in Prostate benign hyperplasia and carcinoma
Nutrition and Cancer, 2003Co-Authors: Phyllis E. Bowen, Longwen Chen, Claudine Duncan, Luna Ghosh, Roohollah Sharifi, Konstantin ChristovAbstract:Population studies have suggested that lycopene, which is mostly found in tomato and tomato products, may reduce the risk of Prostate cancer. We previously found that tomato sauce consumption prior to Prostatectomy for Prostate cancer decreased serum Prostate specific antigen, decreased oxidative DNA damage, and increased lycopene concentrations in Prostate Tissue (Chen et al., 2001). Here, we extended those investigations to determine whether apoptotic cell death and associated Bcl-2 and Bax proteins were modulated by tomato sauce intervention. Thirty-two patients diagnosed by biopsy with Prostate carcinoma were given tomato sauce pasta entrees (30 mg lycopene/day) for 3 wk before Prostatectomy. Thirty-four patients with Prostate cancer who did not consume tomato sauce and underwent Prostatectomy served as controls. When tumor areas with the most apoptotic cells were compared in the biopsy (before) and resected Prostate Tissue (after), tomato sauce consumption increased apoptotic cells in benign Prostate...
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liquid chromatography mass spectrometry of cis and all trans lycopene in human serum and Prostate Tissue after dietary supplementation with tomato sauce
Journal of Agricultural and Food Chemistry, 2002Co-Authors: Richard B Van Breemen, Longwen Chen, Claudine Duncan, Maria Stacewiczsapuntzakis, Marlos A G Viana, Xiaoying Xu, Phyllis E. Bowen, Roohollah SharifiAbstract:Several epidemiological studies suggest a lower incidence of Prostate cancer in men who routinely consume tomato products. Tomatoes are the primary dietary source of lycopene, which is among the most potent antioxidants of the carotenoids. Men with clinical stage T1 or T2 Prostate adenocarcinoma were recruited (n = 32) and consumed tomato sauce based pasta dishes for 3 weeks (equivalent to 30 mg of lycopene per day) before radical prostectomy. Prostate Tissue from needle biopsy just before intervention and prostectomy after supplementation from a subset of 11 subjects was evaluated for both total lycopene and lycopene geometrical isomer ratios. A gradient HPLC system using a C(18) column with UV-vis absorbance detection was used to measure total lycopene. Because the absorbance detector was insufficiently sensitive, HPLC with a C(30) column and positive ion atmospheric pressure chemical ionization mass spectrometric (LC-MS) detection was developed as a new assay to measure the ratio of lycopene cis/trans isomers in these samples. The limit of detection of the LC-MS method was determined to be 0.93 pmol of lycopene on-column, and a linear response was obtained over 3 orders of magnitude. Total lycopene in serum increased 2.0-fold from 35.6 to 69.9 microg/dL (from 0.664 to 1.30 microM) as a result of dietary supplementation with tomato sauce, whereas total lycopene in Prostate Tissue increased 3.0-fold from 0.196 to 0.582 ng/mg of Tissue (from 0.365 to 1.09 pmol/mg). all-trans-Lycopene and at least 14 cis-isomer peaks were detected in Prostate Tissue and serum. The mean proportion of all-trans-lycopene in Prostate Tissue was approximately 12.4% of total lycopene before supplementation but increased to 22.7% after dietary intervention with tomato sauce. In serum there was only a 2.8% but statistically significant increase in the proportion of all-trans-lycopene after intervention. These results indicate that short-term supplementation with tomato sauce containing primarily all-trans-lycopene (83% of total lycopene) results in substantial increases in total lycopene in serum and Prostate and a substantial increase in all-trans-lycopene in Prostate but relatively less in serum.
Andrew Evans - One of the best experts on this subject based on the ideXlab platform.
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Therapy‐associated effects in the Prostate gland
Histopathology, 2011Co-Authors: John R Srigley, Brett Delahunt, Andrew EvansAbstract:Srigley J R, Delahunt B & Evans A J (2012) Histopathology 60, 153–165 Therapy-associated effects in the Prostate gland Diverse therapies are used to treat both benign prostatic hyperplasia and adenocarcinoma. Transurethral resection, a common surgical procedure, may give rise to characteristic necrobiotic granulomas that manifest in subsequent pathology samples. Radiation and hormone therapy have traditionally been used in prostatic adenocarcinoma. Morphological effects are often identified in needle biopsy specimens, transurethral resectates, and radical Prostatectomy specimens. A range of histological changes are noted in the non-neoplastic Prostate Tissue, as well as in the pre-neoplastic and carcinomatous areas. Other ablative therapies, such as cryotherapy, and emerging focal therapies, including high-intensity focused ultrasound, photodynamic therapy, and interstitial laser thermotherapy, may have morphological effects on Prostate Tissue. It is important for the pathologist to be aware of the spectrum of histological changes affecting the Prostate gland post-therapy. The treatment effects may obscure residual carcinoma, and make measurements of tumour extent and stage difficult. Furthermore, some therapies can profoundly alter the neoplastic glands to such an extent that Gleason scoring is no longer valid. As new therapies are developed for Prostate cancer, it is important to document their effects on benign and malignant Prostate Tissue and to understand possible implications for traditional prognostic factors, especially Gleason grade.
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Treatment effects in the Prostate including those associated with traditional and emerging therapies.
Advances in Anatomic Pathology, 2011Co-Authors: Andrew Evans, Paul Ryan, Theodorus Van DerkwastAbstract:Classic treatment options for Prostate cancer consist of radical Prostatectomy, antiandrogen (or hormonal) therapy, and radiation therapy. Hormonal and radiation therapy, in particular, have well known, often profound effects on the histologic appearance of benign Prostate Tissue and prostatic carcinoma. The Tissue changes induced by these treatments have been comprehensively described in several sources. Novel therapies ranging from focal ablative treatments to highly targeted molecular therapies are beginning to emerge and pathologists will play a central role in documenting the effects of these treatments on normal and malignant Prostate Tissue. It is therefore important that pathologists have access to basic treatment information and a solid working knowledge of the morphologic changes induced by these therapies. This will ensure accurate interpretation and reporting of posttreatment Prostate specimens. This review is based on a presentation given by Dr A. Evans at the International Society of Urological Pathology Companion Society Meeting (Hot Topics in Urological Pathology) at The United States Canadian Academy of Pathology Meeting in Washington DC on March 20, 2010. This review will cover the histopathologic features seen in benign Prostate Tissue and prostatic carcinoma seen following: hormonal therapy, radiation therapy, ablative therapies such as vascular-targeted photodynamic therapy, interstitial laser thermotherapy, and high-intensity focussed ultrasound. An emphasis is placed on these specific modalities as they are currently in use as primary, salvage, or investigational therapy in the treatment of Prostate cancer.
Longwen Chen - One of the best experts on this subject based on the ideXlab platform.
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effects of tomato sauce consumption on apoptotic cell death in Prostate benign hyperplasia and carcinoma
Nutrition and Cancer, 2003Co-Authors: Phyllis E. Bowen, Longwen Chen, Claudine Duncan, Luna Ghosh, Roohollah Sharifi, Konstantin ChristovAbstract:Population studies have suggested that lycopene, which is mostly found in tomato and tomato products, may reduce the risk of Prostate cancer. We previously found that tomato sauce consumption prior to Prostatectomy for Prostate cancer decreased serum Prostate specific antigen, decreased oxidative DNA damage, and increased lycopene concentrations in Prostate Tissue (Chen et al., 2001). Here, we extended those investigations to determine whether apoptotic cell death and associated Bcl-2 and Bax proteins were modulated by tomato sauce intervention. Thirty-two patients diagnosed by biopsy with Prostate carcinoma were given tomato sauce pasta entrees (30 mg lycopene/day) for 3 wk before Prostatectomy. Thirty-four patients with Prostate cancer who did not consume tomato sauce and underwent Prostatectomy served as controls. When tumor areas with the most apoptotic cells were compared in the biopsy (before) and resected Prostate Tissue (after), tomato sauce consumption increased apoptotic cells in benign Prostate...
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liquid chromatography mass spectrometry of cis and all trans lycopene in human serum and Prostate Tissue after dietary supplementation with tomato sauce
Journal of Agricultural and Food Chemistry, 2002Co-Authors: Richard B Van Breemen, Longwen Chen, Claudine Duncan, Maria Stacewiczsapuntzakis, Marlos A G Viana, Xiaoying Xu, Phyllis E. Bowen, Roohollah SharifiAbstract:Several epidemiological studies suggest a lower incidence of Prostate cancer in men who routinely consume tomato products. Tomatoes are the primary dietary source of lycopene, which is among the most potent antioxidants of the carotenoids. Men with clinical stage T1 or T2 Prostate adenocarcinoma were recruited (n = 32) and consumed tomato sauce based pasta dishes for 3 weeks (equivalent to 30 mg of lycopene per day) before radical prostectomy. Prostate Tissue from needle biopsy just before intervention and prostectomy after supplementation from a subset of 11 subjects was evaluated for both total lycopene and lycopene geometrical isomer ratios. A gradient HPLC system using a C(18) column with UV-vis absorbance detection was used to measure total lycopene. Because the absorbance detector was insufficiently sensitive, HPLC with a C(30) column and positive ion atmospheric pressure chemical ionization mass spectrometric (LC-MS) detection was developed as a new assay to measure the ratio of lycopene cis/trans isomers in these samples. The limit of detection of the LC-MS method was determined to be 0.93 pmol of lycopene on-column, and a linear response was obtained over 3 orders of magnitude. Total lycopene in serum increased 2.0-fold from 35.6 to 69.9 microg/dL (from 0.664 to 1.30 microM) as a result of dietary supplementation with tomato sauce, whereas total lycopene in Prostate Tissue increased 3.0-fold from 0.196 to 0.582 ng/mg of Tissue (from 0.365 to 1.09 pmol/mg). all-trans-Lycopene and at least 14 cis-isomer peaks were detected in Prostate Tissue and serum. The mean proportion of all-trans-lycopene in Prostate Tissue was approximately 12.4% of total lycopene before supplementation but increased to 22.7% after dietary intervention with tomato sauce. In serum there was only a 2.8% but statistically significant increase in the proportion of all-trans-lycopene after intervention. These results indicate that short-term supplementation with tomato sauce containing primarily all-trans-lycopene (83% of total lycopene) results in substantial increases in total lycopene in serum and Prostate and a substantial increase in all-trans-lycopene in Prostate but relatively less in serum.
Claudine Duncan - One of the best experts on this subject based on the ideXlab platform.
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effects of tomato sauce consumption on apoptotic cell death in Prostate benign hyperplasia and carcinoma
Nutrition and Cancer, 2003Co-Authors: Phyllis E. Bowen, Longwen Chen, Claudine Duncan, Luna Ghosh, Roohollah Sharifi, Konstantin ChristovAbstract:Population studies have suggested that lycopene, which is mostly found in tomato and tomato products, may reduce the risk of Prostate cancer. We previously found that tomato sauce consumption prior to Prostatectomy for Prostate cancer decreased serum Prostate specific antigen, decreased oxidative DNA damage, and increased lycopene concentrations in Prostate Tissue (Chen et al., 2001). Here, we extended those investigations to determine whether apoptotic cell death and associated Bcl-2 and Bax proteins were modulated by tomato sauce intervention. Thirty-two patients diagnosed by biopsy with Prostate carcinoma were given tomato sauce pasta entrees (30 mg lycopene/day) for 3 wk before Prostatectomy. Thirty-four patients with Prostate cancer who did not consume tomato sauce and underwent Prostatectomy served as controls. When tumor areas with the most apoptotic cells were compared in the biopsy (before) and resected Prostate Tissue (after), tomato sauce consumption increased apoptotic cells in benign Prostate...
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liquid chromatography mass spectrometry of cis and all trans lycopene in human serum and Prostate Tissue after dietary supplementation with tomato sauce
Journal of Agricultural and Food Chemistry, 2002Co-Authors: Richard B Van Breemen, Longwen Chen, Claudine Duncan, Maria Stacewiczsapuntzakis, Marlos A G Viana, Xiaoying Xu, Phyllis E. Bowen, Roohollah SharifiAbstract:Several epidemiological studies suggest a lower incidence of Prostate cancer in men who routinely consume tomato products. Tomatoes are the primary dietary source of lycopene, which is among the most potent antioxidants of the carotenoids. Men with clinical stage T1 or T2 Prostate adenocarcinoma were recruited (n = 32) and consumed tomato sauce based pasta dishes for 3 weeks (equivalent to 30 mg of lycopene per day) before radical prostectomy. Prostate Tissue from needle biopsy just before intervention and prostectomy after supplementation from a subset of 11 subjects was evaluated for both total lycopene and lycopene geometrical isomer ratios. A gradient HPLC system using a C(18) column with UV-vis absorbance detection was used to measure total lycopene. Because the absorbance detector was insufficiently sensitive, HPLC with a C(30) column and positive ion atmospheric pressure chemical ionization mass spectrometric (LC-MS) detection was developed as a new assay to measure the ratio of lycopene cis/trans isomers in these samples. The limit of detection of the LC-MS method was determined to be 0.93 pmol of lycopene on-column, and a linear response was obtained over 3 orders of magnitude. Total lycopene in serum increased 2.0-fold from 35.6 to 69.9 microg/dL (from 0.664 to 1.30 microM) as a result of dietary supplementation with tomato sauce, whereas total lycopene in Prostate Tissue increased 3.0-fold from 0.196 to 0.582 ng/mg of Tissue (from 0.365 to 1.09 pmol/mg). all-trans-Lycopene and at least 14 cis-isomer peaks were detected in Prostate Tissue and serum. The mean proportion of all-trans-lycopene in Prostate Tissue was approximately 12.4% of total lycopene before supplementation but increased to 22.7% after dietary intervention with tomato sauce. In serum there was only a 2.8% but statistically significant increase in the proportion of all-trans-lycopene after intervention. These results indicate that short-term supplementation with tomato sauce containing primarily all-trans-lycopene (83% of total lycopene) results in substantial increases in total lycopene in serum and Prostate and a substantial increase in all-trans-lycopene in Prostate but relatively less in serum.
