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Takeki Tsutsui - One of the best experts on this subject based on the ideXlab platform.

  • assessment of genotoxicity of 14 chemical agents used in dental practice ability to induce Chromosome Aberrations in syrian hamster embryo cells
    Mutation Research-genetic Toxicology and Environmental Mutagenesis, 2006
    Co-Authors: Makoto Hagiwara, Eiko Watanabe, Carl J Barrett, Takeki Tsutsui
    Abstract:

    To assess the genotoxicity of 14 chemical agents used as locally applied agents in dental practice, the ability of these agents to elicit Chromosome Aberrations was examined using Syrian hamster embryo (SHE) cells. Chromosome Aberrations in SHE cells were induced by treatment with three of eight chemical agents used as endodontic medicaments, i.e. ethylenediaminetetraacetic acid (EDTA), formocresol (a mixture of formalin and tricresol), and sodium arsenite. The other five chemical agents, i.e. chloramphenicol, p-chlorophenol, p-phenolsulfonic acid, sodium hypochlorite, and tetracycline hydrochloride exhibited a negative response for Chromosome Aberrations. Assessment of three dyes used for disclosing dental plaque showed Chromosome Aberrations induced by basic fuchsin but not by acid fuchsin and erythrosine B. Three local anesthetics, lidocaine hydrochloride, prilocaine hydrochloride, and procaine hydrochloride, were negative for Chromosome Aberrations. Among the ten chemical agents that exhibited a negative response in the assay, p-chlorophenol, sodium hypochlorite, and erythrosine B induced Chromosome Aberrations in SHE cells when treated in the presence of exogenous metabolic activation. The percentages of cells with polyploidy or endoreduplication were enhanced by formocresol, sodium arsenite, p-chlorophenol, p-phenolsulfonic acid, sodium hypochlorite, erythrosine B, prilocaine hydrochloride, and procaine hydrochloride in the absence or presence of exogenous metabolic activation. Our results indicate that the chemical agents that had a positive response in the present study are potentially genotoxic to mammalian cells.

  • ability of fourteen chemical agents used in dental practice to induce Chromosome Aberrations in syrian hamster embryo cells
    Journal of Pharmacological Sciences, 2005
    Co-Authors: Hirohito Hikiba, Eiko Watanabe, Carl J Barrett, Takeki Tsutsui
    Abstract:

    To assess the genotoxicity of 14 chemical agents used in dental practice, the ability of these agents to induce Chromosome Aberrations was examined using Syrian hamster embryo (SHE) cells. Statistically significant increases in the frequencies of Chromosome Aberrations were induced in SHE cells treated with 7 of 10 chemical agents used as endodontic medicaments, that is, carbol camphor, m-cresol, eugenol, guaiacol, zinc oxide, hydrogen peroxide, and formaldehyde. The other 3 chemical agents, that is, thymol, glutaraldehyde, and iodoform, did not increase the levels of Chromosome Aberrations. Of the 4 chemical agents that are used as an antiseptic on the oral mucosa, Chromosome Aberrations were induced by iodine, but not by the other 3 antiseptics, benzalkonium chloride, benzethonium chloride, and chlorhexidine. Among the 6 chemical agents exhibiting a negative response in the assay, only thymol induced Chromosome Aberrations in the presence of exogenous metabolic activation. Our results indicate that chemical agents having a positive response in the present study are potentially genotoxic to mammalian cells and need to be studied further in detail.

N.a. Demopoulos - One of the best experts on this subject based on the ideXlab platform.

J J M Van Dongen - One of the best experts on this subject based on the ideXlab platform.

  • Split-signal FISH for detection of Chromosome Aberrations in acute lymphoblastic leukemia
    Leukemia, 2004
    Co-Authors: M Van Der Burg, A. W. Langerak, T S Poulsen, S P Hunger, H B Beverloo, E M E Smit, K Vang-nielsen, J J M Van Dongen
    Abstract:

    Chromosome Aberrations are frequently observed in precursor-B-acute lymphoblastic leukemias (ALL) and T-cell acute lymphoblastic leukemias (T-ALL). These translocations can form leukemia-specific chimeric fusion proteins or they can deregulate expression of an (onco)gene, resulting in aberrant expression or overexpression. Detection of Chromosome Aberrations is an important tool for risk classification. We developed rapid and sensitive split-signal fluorescent in situ hybridization (FISH) assays for six of the most frequent Chromosome Aberrations in precursor-B-ALL and T-ALL. The split-signal FISH approach uses two differentially labeled probes, located in one gene at opposite sites of the breakpoint region. Probe sets were developed for the genes TCF3 (E2A) at 19p13, MLL at 11q23, ETV6 at 12p13, BCR at 22q11, SIL-TAL1 at 1q32 and TLX3 (HOX11L2) at 5q35. In normal karyotypes, two colocalized green/red signals are visible, but a translocation results in a split of one of the colocalized signals. Split-signal FISH has three main advantages over the classical fusion-signal FISH approach, which uses two labeled probes located in two genes. First, the detection of a Chromosome aberration is independent of the involved partner gene. Second, split-signal FISH allows the identification of the partner gene or Chromosome region if metaphase spreads are present, and finally it reduces false-positivity.

Carl J Barrett - One of the best experts on this subject based on the ideXlab platform.

  • assessment of genotoxicity of 14 chemical agents used in dental practice ability to induce Chromosome Aberrations in syrian hamster embryo cells
    Mutation Research-genetic Toxicology and Environmental Mutagenesis, 2006
    Co-Authors: Makoto Hagiwara, Eiko Watanabe, Carl J Barrett, Takeki Tsutsui
    Abstract:

    To assess the genotoxicity of 14 chemical agents used as locally applied agents in dental practice, the ability of these agents to elicit Chromosome Aberrations was examined using Syrian hamster embryo (SHE) cells. Chromosome Aberrations in SHE cells were induced by treatment with three of eight chemical agents used as endodontic medicaments, i.e. ethylenediaminetetraacetic acid (EDTA), formocresol (a mixture of formalin and tricresol), and sodium arsenite. The other five chemical agents, i.e. chloramphenicol, p-chlorophenol, p-phenolsulfonic acid, sodium hypochlorite, and tetracycline hydrochloride exhibited a negative response for Chromosome Aberrations. Assessment of three dyes used for disclosing dental plaque showed Chromosome Aberrations induced by basic fuchsin but not by acid fuchsin and erythrosine B. Three local anesthetics, lidocaine hydrochloride, prilocaine hydrochloride, and procaine hydrochloride, were negative for Chromosome Aberrations. Among the ten chemical agents that exhibited a negative response in the assay, p-chlorophenol, sodium hypochlorite, and erythrosine B induced Chromosome Aberrations in SHE cells when treated in the presence of exogenous metabolic activation. The percentages of cells with polyploidy or endoreduplication were enhanced by formocresol, sodium arsenite, p-chlorophenol, p-phenolsulfonic acid, sodium hypochlorite, erythrosine B, prilocaine hydrochloride, and procaine hydrochloride in the absence or presence of exogenous metabolic activation. Our results indicate that the chemical agents that had a positive response in the present study are potentially genotoxic to mammalian cells.

  • ability of fourteen chemical agents used in dental practice to induce Chromosome Aberrations in syrian hamster embryo cells
    Journal of Pharmacological Sciences, 2005
    Co-Authors: Hirohito Hikiba, Eiko Watanabe, Carl J Barrett, Takeki Tsutsui
    Abstract:

    To assess the genotoxicity of 14 chemical agents used in dental practice, the ability of these agents to induce Chromosome Aberrations was examined using Syrian hamster embryo (SHE) cells. Statistically significant increases in the frequencies of Chromosome Aberrations were induced in SHE cells treated with 7 of 10 chemical agents used as endodontic medicaments, that is, carbol camphor, m-cresol, eugenol, guaiacol, zinc oxide, hydrogen peroxide, and formaldehyde. The other 3 chemical agents, that is, thymol, glutaraldehyde, and iodoform, did not increase the levels of Chromosome Aberrations. Of the 4 chemical agents that are used as an antiseptic on the oral mucosa, Chromosome Aberrations were induced by iodine, but not by the other 3 antiseptics, benzalkonium chloride, benzethonium chloride, and chlorhexidine. Among the 6 chemical agents exhibiting a negative response in the assay, only thymol induced Chromosome Aberrations in the presence of exogenous metabolic activation. Our results indicate that chemical agents having a positive response in the present study are potentially genotoxic to mammalian cells and need to be studied further in detail.

Eiko Watanabe - One of the best experts on this subject based on the ideXlab platform.

  • assessment of genotoxicity of 14 chemical agents used in dental practice ability to induce Chromosome Aberrations in syrian hamster embryo cells
    Mutation Research-genetic Toxicology and Environmental Mutagenesis, 2006
    Co-Authors: Makoto Hagiwara, Eiko Watanabe, Carl J Barrett, Takeki Tsutsui
    Abstract:

    To assess the genotoxicity of 14 chemical agents used as locally applied agents in dental practice, the ability of these agents to elicit Chromosome Aberrations was examined using Syrian hamster embryo (SHE) cells. Chromosome Aberrations in SHE cells were induced by treatment with three of eight chemical agents used as endodontic medicaments, i.e. ethylenediaminetetraacetic acid (EDTA), formocresol (a mixture of formalin and tricresol), and sodium arsenite. The other five chemical agents, i.e. chloramphenicol, p-chlorophenol, p-phenolsulfonic acid, sodium hypochlorite, and tetracycline hydrochloride exhibited a negative response for Chromosome Aberrations. Assessment of three dyes used for disclosing dental plaque showed Chromosome Aberrations induced by basic fuchsin but not by acid fuchsin and erythrosine B. Three local anesthetics, lidocaine hydrochloride, prilocaine hydrochloride, and procaine hydrochloride, were negative for Chromosome Aberrations. Among the ten chemical agents that exhibited a negative response in the assay, p-chlorophenol, sodium hypochlorite, and erythrosine B induced Chromosome Aberrations in SHE cells when treated in the presence of exogenous metabolic activation. The percentages of cells with polyploidy or endoreduplication were enhanced by formocresol, sodium arsenite, p-chlorophenol, p-phenolsulfonic acid, sodium hypochlorite, erythrosine B, prilocaine hydrochloride, and procaine hydrochloride in the absence or presence of exogenous metabolic activation. Our results indicate that the chemical agents that had a positive response in the present study are potentially genotoxic to mammalian cells.

  • ability of fourteen chemical agents used in dental practice to induce Chromosome Aberrations in syrian hamster embryo cells
    Journal of Pharmacological Sciences, 2005
    Co-Authors: Hirohito Hikiba, Eiko Watanabe, Carl J Barrett, Takeki Tsutsui
    Abstract:

    To assess the genotoxicity of 14 chemical agents used in dental practice, the ability of these agents to induce Chromosome Aberrations was examined using Syrian hamster embryo (SHE) cells. Statistically significant increases in the frequencies of Chromosome Aberrations were induced in SHE cells treated with 7 of 10 chemical agents used as endodontic medicaments, that is, carbol camphor, m-cresol, eugenol, guaiacol, zinc oxide, hydrogen peroxide, and formaldehyde. The other 3 chemical agents, that is, thymol, glutaraldehyde, and iodoform, did not increase the levels of Chromosome Aberrations. Of the 4 chemical agents that are used as an antiseptic on the oral mucosa, Chromosome Aberrations were induced by iodine, but not by the other 3 antiseptics, benzalkonium chloride, benzethonium chloride, and chlorhexidine. Among the 6 chemical agents exhibiting a negative response in the assay, only thymol induced Chromosome Aberrations in the presence of exogenous metabolic activation. Our results indicate that chemical agents having a positive response in the present study are potentially genotoxic to mammalian cells and need to be studied further in detail.