The Experts below are selected from a list of 96 Experts worldwide ranked by ideXlab platform

Jan Zima - One of the best experts on this subject based on the ideXlab platform.

  • Research Article Comparison of the Chromosome Banding Pattern in the 2
    2016
    Co-Authors: Xanthodon From N. Turkey, Atilla Arslan, Apdil Arjsoy, Jan Zima
    Abstract:

    Copyright © 2014 Atilla Arslan et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. We present the karyotype characteristics of five cytotypes of mole rats (Nannospalax) with 56 Chromosomes revealed by the C-Banding and AgNOR staining analyses. We attempt to investigate if the specific distinction between the populations fromThrace (N. leucodon) and Anatolia (N. xanthodon) is reflected also in their karyotypic differentiation. The specimens from each of the five populations studied revealed a distinct karyotype which was different from those found in other populations. The fundamental number of autosomal arms varied from 68 to 72.The amount of C-heterochromatin was larger in theThrace sample ofN. leucodon compared to the Anatolian population ofN. xanthodon.The active NOR sites were recorded on five autosomal pairs inN. leucodon, whereas only three or four pairs bearing NOR were observed in N. xanthodon. Differences between the studied populations were quantified in the analysis of the distribution Pattern of the C-positive bands and the AgNOR sites in individual Chromosomes which indicated a basal position of the Thrace sample of N. leucodon and its divergence from other studied populations. The karyotypes of the 56-Chromosome populations of N. leucodon and N. xanthodon are thus distinctly different. 1. Introduction. The mole rats (Muridae, Spalacinae) [1] represent an attrac-tive model for various biological and evolutionary studie

  • Chromosome Banding Pattern retrieves an independent origin of 2n 50 Chromosome populations of nannospalax xanthodon from turkey
    Mammalian Biology, 2015
    Co-Authors: Atilla Arslan, Jan Zima
    Abstract:

    We present the karyotype characteristics of four populations of mole rats (Nannospalax xanthodon) with 50 Chromosomes investigated by C-Banding and AgNOR staining. The populations studied originated from the Asiatic part of Turkey and the collecting sites were separated by geographic distance of several hundreds of kilometres. The comparative analysis showed that these populations differ, in spite of the identical diploid number of Chromosomes, in various discrete cytogenetic markers. The overall amount of C-heterochromatin varied between populations, and also variation in the number and localization of C-heterochromatic autosomal short arms was observed. The nucleolar organizer regions occurred on two, three or four autosomal pairs. They were found usually in the telomeric position and were associated with the C-heterochromatic arms. These results indicate that a cytotype with the particular number of Chromosomes may include several populations with different karyotypes that have evolved independently. We therefore assume that convergent evolution of karyotypes with the same diploid Chromosome number is not exceptional in mole rats.

  • Comparison of the Chromosome Banding Pattern in the 2n = 56 cytotypes of Nannospalax leucodon and N. xanthodon from Turkey.
    TheScientificWorldJournal, 2014
    Co-Authors: Atilla Arslan, Apdil Arısoy, Jan Zima
    Abstract:

    We present the karyotype characteristics of five cytotypes of mole rats (Nannospalax) with 56 Chromosomes revealed by the C-Banding and AgNOR staining analyses. We attempt to investigate if the specific distinction between the populations from Thrace (N. leucodon) and Anatolia (N. xanthodon) is reflected also in their karyotypic differentiation. The specimens from each of the five populations studied revealed a distinct karyotype which was different from those found in other populations. The fundamental number of autosomal arms varied from 68 to 72. The amount of C-heterochromatin was larger in the Thrace sample of N. leucodon compared to the Anatolian population of N. xanthodon. The active NOR sites were recorded on five autosomal pairs in N. leucodon, whereas only three or four pairs bearing NOR were observed in N. xanthodon. Differences between the studied populations were quantified in the analysis of the distribution Pattern of the C-positive bands and the AgNOR sites in individual Chromosomes which indicated a basal position of the Thrace sample of N. leucodon and its divergence from other studied populations. The karyotypes of the 56-Chromosome populations of N. leucodon and N. xanthodon are thus distinctly different.

  • comparison of the Chromosome Banding Pattern in the 2n 56 cytotypes of nannospalax leucodon and n xanthodon from turkey
    The Scientific World Journal, 2014
    Co-Authors: Atilla Arslan, Apdil Arısoy, Jan Zima
    Abstract:

    We present the karyotype characteristics of five cytotypes of mole rats (Nannospalax) with 56 Chromosomes revealed by the C-Banding and AgNOR staining analyses. We attempt to investigate if the specific distinction between the populations from Thrace (N. leucodon) and Anatolia (N. xanthodon) is reflected also in their karyotypic differentiation. The specimens from each of the five populations studied revealed a distinct karyotype which was different from those found in other populations. The fundamental number of autosomal arms varied from 68 to 72. The amount of C-heterochromatin was larger in the Thrace sample of N. leucodon compared to the Anatolian population of N. xanthodon. The active NOR sites were recorded on five autosomal pairs in N. leucodon, whereas only three or four pairs bearing NOR were observed in N. xanthodon. Differences between the studied populations were quantified in the analysis of the distribution Pattern of the C-positive bands and the AgNOR sites in individual Chromosomes which indicated a basal position of the Thrace sample of N. leucodon and its divergence from other studied populations. The karyotypes of the 56-Chromosome populations of N. leucodon and N. xanthodon are thus distinctly different.

  • The Chromosome Banding Pattern in two cytotypes (2n = 36 and 38) of blind mole rats from Turkey (Mammalia: Spalaxidae)
    Zoology in the Middle East, 2013
    Co-Authors: Atilla Arslan, Apdil Arısoy, Jan Zima
    Abstract:

    Two cytotypes (2n = 36 and 38) of blind mole rats, Nannospalax xanthodon (Nordmann, 1840), from the Aydin and Manisa provinces in Turkey were investigated. Conventional Chromosome staining, Ag-NOR staining and C-Banding analysis were carried out. From the cytogenetic point of view, the particular phylogenetic position of these populations is supported by their low diploid numbers only, and the CBanding Pattern and the NORs distribution seem generally similar to populations with higher Chromosome numbers. Several autosomal pairs with centromeric dark Cbands were observed in the 2n=36 cytotype. One autosomal pair possessed an interstitial dark C-band on the short arm; another pair possessed an interstitial dark Cband on the long arm. Whole C-heterochromatic short arms were observed in three subtelocentric autosomal pairs in the 2n=38 cytotype. Most of the other autosomal pairs possessed centromeric dark C-bands. Distinct dark C-bands were observed also in the presumed X Chromosomes of both the cytotypes. Th...

Muhammad Shoaib - One of the best experts on this subject based on the ideXlab platform.

  • Topokaryotyping demonstrates single cell variability and stress dependent variations in nuclear envelope associated domains
    Nucleic Acids Research, 2018
    Co-Authors: Anamarija Jurisic, Chloé Robin, Pavel Tarlykov, Lee Siggens, Brigitte Schoell, Anna Jauch, Karl Ekwall, Claus Storgaard Sørensen, Marc Lipinski, Muhammad Shoaib
    Abstract:

    Analysis of large-scale interphase genome positioning with reference to a nuclear landmark has recently been studied using sequencing-based single cell approaches. However, these approaches are dependent upon technically challenging, time consuming and costly high throughput sequencing technologies, requiring specialized bioinformatics tools and expertise. Here, we propose a novel, affordable and robust microscopy-based single cell approach, termed Topokaryotyping, to analyze and reconstruct the interphase positioning of genomic loci relative to a given nuclear landmark, detectable as Banding Pattern on mitotic Chromosomes. This is accomplished by proximity-dependent histone labeling, where biotin ligase BirA fused to nuclear envelope marker Emerin was coexpressed together with Biotin Acceptor Peptide (BAP)-histone fusion followed by (i) biotin labeling, (ii) generation of mitotic spreads, (iii) detection of the biotin label on mitotic Chromosomes and (iv) their identification by karyotyping. Using Topokaryotyping, we identified both cooperativity and stochasticity in the positioning of emerin-associated chromatin domains in individual cells. Furthermore, the Chromosome-Banding Pattern showed dynamic changes in emerin-associated domains upon physical and radiological stress. In summary, Topokaryotyping is a sensitive and reliable technique to quantitatively analyze spatial positioning of genomic regions interacting with a given nuclear landmark at the single cell level in various experimental conditions.

Atilla Arslan - One of the best experts on this subject based on the ideXlab platform.

  • Research Article Comparison of the Chromosome Banding Pattern in the 2
    2016
    Co-Authors: Xanthodon From N. Turkey, Atilla Arslan, Apdil Arjsoy, Jan Zima
    Abstract:

    Copyright © 2014 Atilla Arslan et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. We present the karyotype characteristics of five cytotypes of mole rats (Nannospalax) with 56 Chromosomes revealed by the C-Banding and AgNOR staining analyses. We attempt to investigate if the specific distinction between the populations fromThrace (N. leucodon) and Anatolia (N. xanthodon) is reflected also in their karyotypic differentiation. The specimens from each of the five populations studied revealed a distinct karyotype which was different from those found in other populations. The fundamental number of autosomal arms varied from 68 to 72.The amount of C-heterochromatin was larger in theThrace sample ofN. leucodon compared to the Anatolian population ofN. xanthodon.The active NOR sites were recorded on five autosomal pairs inN. leucodon, whereas only three or four pairs bearing NOR were observed in N. xanthodon. Differences between the studied populations were quantified in the analysis of the distribution Pattern of the C-positive bands and the AgNOR sites in individual Chromosomes which indicated a basal position of the Thrace sample of N. leucodon and its divergence from other studied populations. The karyotypes of the 56-Chromosome populations of N. leucodon and N. xanthodon are thus distinctly different. 1. Introduction. The mole rats (Muridae, Spalacinae) [1] represent an attrac-tive model for various biological and evolutionary studie

  • Chromosome Banding Pattern retrieves an independent origin of 2n 50 Chromosome populations of nannospalax xanthodon from turkey
    Mammalian Biology, 2015
    Co-Authors: Atilla Arslan, Jan Zima
    Abstract:

    We present the karyotype characteristics of four populations of mole rats (Nannospalax xanthodon) with 50 Chromosomes investigated by C-Banding and AgNOR staining. The populations studied originated from the Asiatic part of Turkey and the collecting sites were separated by geographic distance of several hundreds of kilometres. The comparative analysis showed that these populations differ, in spite of the identical diploid number of Chromosomes, in various discrete cytogenetic markers. The overall amount of C-heterochromatin varied between populations, and also variation in the number and localization of C-heterochromatic autosomal short arms was observed. The nucleolar organizer regions occurred on two, three or four autosomal pairs. They were found usually in the telomeric position and were associated with the C-heterochromatic arms. These results indicate that a cytotype with the particular number of Chromosomes may include several populations with different karyotypes that have evolved independently. We therefore assume that convergent evolution of karyotypes with the same diploid Chromosome number is not exceptional in mole rats.

  • Comparison of the Chromosome Banding Pattern in the 2n = 56 cytotypes of Nannospalax leucodon and N. xanthodon from Turkey.
    TheScientificWorldJournal, 2014
    Co-Authors: Atilla Arslan, Apdil Arısoy, Jan Zima
    Abstract:

    We present the karyotype characteristics of five cytotypes of mole rats (Nannospalax) with 56 Chromosomes revealed by the C-Banding and AgNOR staining analyses. We attempt to investigate if the specific distinction between the populations from Thrace (N. leucodon) and Anatolia (N. xanthodon) is reflected also in their karyotypic differentiation. The specimens from each of the five populations studied revealed a distinct karyotype which was different from those found in other populations. The fundamental number of autosomal arms varied from 68 to 72. The amount of C-heterochromatin was larger in the Thrace sample of N. leucodon compared to the Anatolian population of N. xanthodon. The active NOR sites were recorded on five autosomal pairs in N. leucodon, whereas only three or four pairs bearing NOR were observed in N. xanthodon. Differences between the studied populations were quantified in the analysis of the distribution Pattern of the C-positive bands and the AgNOR sites in individual Chromosomes which indicated a basal position of the Thrace sample of N. leucodon and its divergence from other studied populations. The karyotypes of the 56-Chromosome populations of N. leucodon and N. xanthodon are thus distinctly different.

  • comparison of the Chromosome Banding Pattern in the 2n 56 cytotypes of nannospalax leucodon and n xanthodon from turkey
    The Scientific World Journal, 2014
    Co-Authors: Atilla Arslan, Apdil Arısoy, Jan Zima
    Abstract:

    We present the karyotype characteristics of five cytotypes of mole rats (Nannospalax) with 56 Chromosomes revealed by the C-Banding and AgNOR staining analyses. We attempt to investigate if the specific distinction between the populations from Thrace (N. leucodon) and Anatolia (N. xanthodon) is reflected also in their karyotypic differentiation. The specimens from each of the five populations studied revealed a distinct karyotype which was different from those found in other populations. The fundamental number of autosomal arms varied from 68 to 72. The amount of C-heterochromatin was larger in the Thrace sample of N. leucodon compared to the Anatolian population of N. xanthodon. The active NOR sites were recorded on five autosomal pairs in N. leucodon, whereas only three or four pairs bearing NOR were observed in N. xanthodon. Differences between the studied populations were quantified in the analysis of the distribution Pattern of the C-positive bands and the AgNOR sites in individual Chromosomes which indicated a basal position of the Thrace sample of N. leucodon and its divergence from other studied populations. The karyotypes of the 56-Chromosome populations of N. leucodon and N. xanthodon are thus distinctly different.

  • The Chromosome Banding Pattern in two cytotypes (2n = 36 and 38) of blind mole rats from Turkey (Mammalia: Spalaxidae)
    Zoology in the Middle East, 2013
    Co-Authors: Atilla Arslan, Apdil Arısoy, Jan Zima
    Abstract:

    Two cytotypes (2n = 36 and 38) of blind mole rats, Nannospalax xanthodon (Nordmann, 1840), from the Aydin and Manisa provinces in Turkey were investigated. Conventional Chromosome staining, Ag-NOR staining and C-Banding analysis were carried out. From the cytogenetic point of view, the particular phylogenetic position of these populations is supported by their low diploid numbers only, and the CBanding Pattern and the NORs distribution seem generally similar to populations with higher Chromosome numbers. Several autosomal pairs with centromeric dark Cbands were observed in the 2n=36 cytotype. One autosomal pair possessed an interstitial dark C-band on the short arm; another pair possessed an interstitial dark Cband on the long arm. Whole C-heterochromatic short arms were observed in three subtelocentric autosomal pairs in the 2n=38 cytotype. Most of the other autosomal pairs possessed centromeric dark C-bands. Distinct dark C-bands were observed also in the presumed X Chromosomes of both the cytotypes. Th...

Anamarija Jurisic - One of the best experts on this subject based on the ideXlab platform.

  • Topokaryotyping demonstrates single cell variability and stress dependent variations in nuclear envelope associated domains
    Nucleic Acids Research, 2018
    Co-Authors: Anamarija Jurisic, Chloé Robin, Pavel Tarlykov, Lee Siggens, Brigitte Schoell, Anna Jauch, Karl Ekwall, Claus Storgaard Sørensen, Marc Lipinski, Muhammad Shoaib
    Abstract:

    Analysis of large-scale interphase genome positioning with reference to a nuclear landmark has recently been studied using sequencing-based single cell approaches. However, these approaches are dependent upon technically challenging, time consuming and costly high throughput sequencing technologies, requiring specialized bioinformatics tools and expertise. Here, we propose a novel, affordable and robust microscopy-based single cell approach, termed Topokaryotyping, to analyze and reconstruct the interphase positioning of genomic loci relative to a given nuclear landmark, detectable as Banding Pattern on mitotic Chromosomes. This is accomplished by proximity-dependent histone labeling, where biotin ligase BirA fused to nuclear envelope marker Emerin was coexpressed together with Biotin Acceptor Peptide (BAP)-histone fusion followed by (i) biotin labeling, (ii) generation of mitotic spreads, (iii) detection of the biotin label on mitotic Chromosomes and (iv) their identification by karyotyping. Using Topokaryotyping, we identified both cooperativity and stochasticity in the positioning of emerin-associated chromatin domains in individual cells. Furthermore, the Chromosome-Banding Pattern showed dynamic changes in emerin-associated domains upon physical and radiological stress. In summary, Topokaryotyping is a sensitive and reliable technique to quantitatively analyze spatial positioning of genomic regions interacting with a given nuclear landmark at the single cell level in various experimental conditions.

M Guerra - One of the best experts on this subject based on the ideXlab platform.

  • Cytogenetics of Rutaceae. V. High chromosomal variability in Citrus species revealed by CMA/DAPI staining
    Heredity, 1993
    Co-Authors: M Guerra
    Abstract:

    The CMA/DAPI Chromosome Banding Pattern of six Citrus species was analysed. All of them showed a large amount of heterochromatin and heterozygosity. Most of the heterochromatin was CMA+/DAPI− and was located mainly in the terminal region of the long arms. Each individual was heterozygous for at least one Chromosome pair and each species presented a different Banding Pattern. The greatest amount of information on karyotype variation can be obtained from the four largest pairs. Pairs I and III showed the highest amount of heterochromatin and heterozygosity whereas pair II was highly stable and almost completely lacked CMA+ heterochromatin. On the other hand, some features common to all species suggest a strong Chromosome homoeology conservation. The CMA staining revealed some Chromosome markers for each Citrus species that may be very useful in the identification of zygotic versus nucellar embryos, as well as for further evolutionary studies.

  • cytogenetics of rutaceae v high chromosomal variability in citrus species revealed by cma dapi staining
    Heredity, 1993
    Co-Authors: M Guerra
    Abstract:

    The CMA/DAPI Chromosome Banding Pattern of six Citrus species was analysed. All of them showed a large amount of heterochromatin and heterozygosity. Most of the heterochromatin was CMA+/DAPI− and was located mainly in the terminal region of the long arms. Each individual was heterozygous for at least one Chromosome pair and each species presented a different Banding Pattern. The greatest amount of information on karyotype variation can be obtained from the four largest pairs. Pairs I and III showed the highest amount of heterochromatin and heterozygosity whereas pair II was highly stable and almost completely lacked CMA+ heterochromatin. On the other hand, some features common to all species suggest a strong Chromosome homoeology conservation. The CMA staining revealed some Chromosome markers for each Citrus species that may be very useful in the identification of zygotic versus nucellar embryos, as well as for further evolutionary studies.