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Yujie Huang - One of the best experts on this subject based on the ideXlab platform.

  • regulated electroporation mediated delivery of pro opiomelanocortin gene suppresses Chronic Constriction Injury induced neuropathic pain in rats
    Gene Therapy, 2004
    Co-Authors: C.m. Wu, Yenling Wang, Jiintsuey Cheng, Yujie Huang
    Abstract:

    Regulated, electroporation-mediated delivery of pro-opiomelanocortin gene suppresses Chronic Constriction Injury-induced neuropathic pain in rats

  • Regulated, electroporation-mediated delivery of pro-opiomelanocortin gene suppresses Chronic Constriction Injury-induced neuropathic pain in rats.
    Gene therapy, 2004
    Co-Authors: Ming-chia Lin, Yenling Wang, Jiintsuey Cheng, Yujie Huang, Wei-zen Sun, Chen-jung Lin
    Abstract:

    We previously reported that intrathecal pro-opiomelanocortin gene electroporation could reduce pain sensitivity induced by Chronic Constriction Injury (CCI) of the sciatic nerve. For optimal use of antinociceptive gene therapy, it might be important to control the expression of the transfected gene extrinsically. For this purpose, a doxycycline-controlled transrepressor system composed of two plasmids coding, respectively, for pro-opiomelanocortin gene (pTRE2-POMC) and the silencer (pTel-off) was employed. The regulation of beta-endorphin expression was first assessed in spinal neuronal culture, then we electrotranfected this plasmid into the spinal cord of mononeuropathic rats and evaluated the analgesic potential of this therapy in vivo by thermal and mechanical withdrawal latency. Intraperitoneal injections of various doses of doxycycline were made to elucidate the possible exogenous downregulation of transfected beta-endorphin gene expression in vivo. The levels of beta-endorphin were analyzed by intrathecal microdialysis and radioimmunoassay. Intrathecal pTRE2-POMC/pTel-off electroporation elevated spinal beta-endorphin levels, as manifested in a significantly elevated pain threshold for Chronic Constriction Injury limbs. Intraperitoneal doxycycline decreased the antinociceptive effect and spinal beta-endorphin levels in a dose-dependent manner. We concluded that intrathecal pTRE2-POMC/pTel-off electroporation alleviates CCI-induced limb pain, and can be controlled by intraperitoneal doxycycline administration.

Yenling Wang - One of the best experts on this subject based on the ideXlab platform.

  • regulated electroporation mediated delivery of pro opiomelanocortin gene suppresses Chronic Constriction Injury induced neuropathic pain in rats
    Gene Therapy, 2004
    Co-Authors: C.m. Wu, Yenling Wang, Jiintsuey Cheng, Yujie Huang
    Abstract:

    Regulated, electroporation-mediated delivery of pro-opiomelanocortin gene suppresses Chronic Constriction Injury-induced neuropathic pain in rats

  • Regulated, electroporation-mediated delivery of pro-opiomelanocortin gene suppresses Chronic Constriction Injury-induced neuropathic pain in rats.
    Gene therapy, 2004
    Co-Authors: Ming-chia Lin, Yenling Wang, Jiintsuey Cheng, Yujie Huang, Wei-zen Sun, Chen-jung Lin
    Abstract:

    We previously reported that intrathecal pro-opiomelanocortin gene electroporation could reduce pain sensitivity induced by Chronic Constriction Injury (CCI) of the sciatic nerve. For optimal use of antinociceptive gene therapy, it might be important to control the expression of the transfected gene extrinsically. For this purpose, a doxycycline-controlled transrepressor system composed of two plasmids coding, respectively, for pro-opiomelanocortin gene (pTRE2-POMC) and the silencer (pTel-off) was employed. The regulation of beta-endorphin expression was first assessed in spinal neuronal culture, then we electrotranfected this plasmid into the spinal cord of mononeuropathic rats and evaluated the analgesic potential of this therapy in vivo by thermal and mechanical withdrawal latency. Intraperitoneal injections of various doses of doxycycline were made to elucidate the possible exogenous downregulation of transfected beta-endorphin gene expression in vivo. The levels of beta-endorphin were analyzed by intrathecal microdialysis and radioimmunoassay. Intrathecal pTRE2-POMC/pTel-off electroporation elevated spinal beta-endorphin levels, as manifested in a significantly elevated pain threshold for Chronic Constriction Injury limbs. Intraperitoneal doxycycline decreased the antinociceptive effect and spinal beta-endorphin levels in a dose-dependent manner. We concluded that intrathecal pTRE2-POMC/pTel-off electroporation alleviates CCI-induced limb pain, and can be controlled by intraperitoneal doxycycline administration.

Jiintsuey Cheng - One of the best experts on this subject based on the ideXlab platform.

  • regulated electroporation mediated delivery of pro opiomelanocortin gene suppresses Chronic Constriction Injury induced neuropathic pain in rats
    Gene Therapy, 2004
    Co-Authors: C.m. Wu, Yenling Wang, Jiintsuey Cheng, Yujie Huang
    Abstract:

    Regulated, electroporation-mediated delivery of pro-opiomelanocortin gene suppresses Chronic Constriction Injury-induced neuropathic pain in rats

  • Regulated, electroporation-mediated delivery of pro-opiomelanocortin gene suppresses Chronic Constriction Injury-induced neuropathic pain in rats.
    Gene therapy, 2004
    Co-Authors: Ming-chia Lin, Yenling Wang, Jiintsuey Cheng, Yujie Huang, Wei-zen Sun, Chen-jung Lin
    Abstract:

    We previously reported that intrathecal pro-opiomelanocortin gene electroporation could reduce pain sensitivity induced by Chronic Constriction Injury (CCI) of the sciatic nerve. For optimal use of antinociceptive gene therapy, it might be important to control the expression of the transfected gene extrinsically. For this purpose, a doxycycline-controlled transrepressor system composed of two plasmids coding, respectively, for pro-opiomelanocortin gene (pTRE2-POMC) and the silencer (pTel-off) was employed. The regulation of beta-endorphin expression was first assessed in spinal neuronal culture, then we electrotranfected this plasmid into the spinal cord of mononeuropathic rats and evaluated the analgesic potential of this therapy in vivo by thermal and mechanical withdrawal latency. Intraperitoneal injections of various doses of doxycycline were made to elucidate the possible exogenous downregulation of transfected beta-endorphin gene expression in vivo. The levels of beta-endorphin were analyzed by intrathecal microdialysis and radioimmunoassay. Intrathecal pTRE2-POMC/pTel-off electroporation elevated spinal beta-endorphin levels, as manifested in a significantly elevated pain threshold for Chronic Constriction Injury limbs. Intraperitoneal doxycycline decreased the antinociceptive effect and spinal beta-endorphin levels in a dose-dependent manner. We concluded that intrathecal pTRE2-POMC/pTel-off electroporation alleviates CCI-induced limb pain, and can be controlled by intraperitoneal doxycycline administration.

C.m. Wu - One of the best experts on this subject based on the ideXlab platform.

Chen-jung Lin - One of the best experts on this subject based on the ideXlab platform.

  • Regulated, electroporation-mediated delivery of pro-opiomelanocortin gene suppresses Chronic Constriction Injury-induced neuropathic pain in rats.
    Gene therapy, 2004
    Co-Authors: Ming-chia Lin, Yenling Wang, Jiintsuey Cheng, Yujie Huang, Wei-zen Sun, Chen-jung Lin
    Abstract:

    We previously reported that intrathecal pro-opiomelanocortin gene electroporation could reduce pain sensitivity induced by Chronic Constriction Injury (CCI) of the sciatic nerve. For optimal use of antinociceptive gene therapy, it might be important to control the expression of the transfected gene extrinsically. For this purpose, a doxycycline-controlled transrepressor system composed of two plasmids coding, respectively, for pro-opiomelanocortin gene (pTRE2-POMC) and the silencer (pTel-off) was employed. The regulation of beta-endorphin expression was first assessed in spinal neuronal culture, then we electrotranfected this plasmid into the spinal cord of mononeuropathic rats and evaluated the analgesic potential of this therapy in vivo by thermal and mechanical withdrawal latency. Intraperitoneal injections of various doses of doxycycline were made to elucidate the possible exogenous downregulation of transfected beta-endorphin gene expression in vivo. The levels of beta-endorphin were analyzed by intrathecal microdialysis and radioimmunoassay. Intrathecal pTRE2-POMC/pTel-off electroporation elevated spinal beta-endorphin levels, as manifested in a significantly elevated pain threshold for Chronic Constriction Injury limbs. Intraperitoneal doxycycline decreased the antinociceptive effect and spinal beta-endorphin levels in a dose-dependent manner. We concluded that intrathecal pTRE2-POMC/pTel-off electroporation alleviates CCI-induced limb pain, and can be controlled by intraperitoneal doxycycline administration.