The Experts below are selected from a list of 360 Experts worldwide ranked by ideXlab platform

Qingyun Zeng - One of the best experts on this subject based on the ideXlab platform.

  • retraction note to Chrysophanol exhibits anti cancer activities in lung cancer cell through regulating ros hif 1a vegf signaling pathway
    Naunyn-schmiedebergs Archives of Pharmacology, 2020
    Co-Authors: Jie Zhang, Qian Wang, Qiang Wang, Peng Guo, Yong Wang, Yuqing Xing, Mengmeng Zhang, Fujun Liu, Qingyun Zeng
    Abstract:

    In the present study, we explored the anti-tumor and anti-angiogenesis effects of Chrysophanol, and to investigate the underlying mechanism of the Chrysophanol on anti-tumor and anti-angiogenesis in human lung cancer. The viability of cells was measured by CCK-8 assay, cell apoptosis was measured by Annexin-FITC/PI staining assay, and the cell migration and invasion were analyzed by wound-healing assay and transwell assay. ROS generation and mitochondrial membrane potential were analyzed by DCFH-DA probe and mitochondrial staining kit. Angiogenesis was analyzed by tube formation assay. The expression of CD31 was analyzed by immunofluorescence. The levels of proteins were measured by western blot assay. The anti-tumor effects of Chrysophanol in vivo were detected by established xenograft mice model. In this study, we found that the cell proliferation, migration, invasion, tube formation, the mitochondrial membrane potential, and the expression of CD31 were inhibited by Chrysophanol in a dose-dependent manner, but cell apoptotic ratios and ROS levels were increased by Chrysophanol in a dose-dependent manner. Furthermore, the effects of Chrysophanol on A549, H738, and HUVEC cell apoptotic rates were reversed by the ROS inhibitor NAC. Besides, the effects of Chrysophanol on HUVEC cell tube formation were reversed by the HIF-1α inhibitor KC7F2 and the VEGF inhibitor axitinib in vitro. Moreover, tumor growth was reduced by Chrysophanol, and the expression of CD31, CD34, and angiogenin was suppressed by Chrysophanol in vivo. Our finding demonstrated that Chrysophanol is a highly effective and low-toxic drug for inhibition of tumor growth especially in high vascularized lung cancer.

  • Chrysophanol exhibits anti cancer activities in lung cancer cell through regulating ros hif 1a vegf signaling pathway
    Naunyn-schmiedebergs Archives of Pharmacology, 2020
    Co-Authors: Jie Zhang, Qian Wang, Qiang Wang, Peng Guo, Yong Wang, Yuqing Xing, Mengmeng Zhang, Fujun Liu, Qingyun Zeng
    Abstract:

    In the present study, we explored the anti-tumor and anti-angiogenesis effects of Chrysophanol, and to investigate the underlying mechanism of the Chrysophanol on anti-tumor and anti-angiogenesis in human lung cancer. The viability of cells was measured by CCK-8 assay, cell apoptosis was measured by Annexin-FITC/PI staining assay, and the cell migration and invasion were analyzed by wound-healing assay and transwell assay. ROS generation and mitochondrial membrane potential were analyzed by DCFH-DA probe and mitochondrial staining kit. Angiogenesis was analyzed by tube formation assay. The expression of CD31 was analyzed by immunofluorescence. The levels of proteins were measured by western blot assay. The anti-tumor effects of Chrysophanol in vivo were detected by established xenograft mice model. In this study, we found that the cell proliferation, migration, invasion, tube formation, the mitochondrial membrane potential, and the expression of CD31 were inhibited by Chrysophanol in a dose-dependent manner, but cell apoptotic ratios and ROS levels were increased by Chrysophanol in a dose-dependent manner. Furthermore, the effects of Chrysophanol on A549, H738, and HUVEC cell apoptotic rates were reversed by the ROS inhibitor NAC. Besides, the effects of Chrysophanol on HUVEC cell tube formation were reversed by the HIF-1α inhibitor KC7F2 and the VEGF inhibitor axitinib in vitro. Moreover, tumor growth was reduced by Chrysophanol, and the expression of CD31, CD34, and angiogenin was suppressed by Chrysophanol in vivo. Our finding demonstrated that Chrysophanol is a highly effective and low-toxic drug for inhibition of tumor growth especially in high vascularized lung cancer.

Qian Wang - One of the best experts on this subject based on the ideXlab platform.

  • retraction note to Chrysophanol exhibits anti cancer activities in lung cancer cell through regulating ros hif 1a vegf signaling pathway
    Naunyn-schmiedebergs Archives of Pharmacology, 2020
    Co-Authors: Jie Zhang, Qian Wang, Qiang Wang, Peng Guo, Yong Wang, Yuqing Xing, Mengmeng Zhang, Fujun Liu, Qingyun Zeng
    Abstract:

    In the present study, we explored the anti-tumor and anti-angiogenesis effects of Chrysophanol, and to investigate the underlying mechanism of the Chrysophanol on anti-tumor and anti-angiogenesis in human lung cancer. The viability of cells was measured by CCK-8 assay, cell apoptosis was measured by Annexin-FITC/PI staining assay, and the cell migration and invasion were analyzed by wound-healing assay and transwell assay. ROS generation and mitochondrial membrane potential were analyzed by DCFH-DA probe and mitochondrial staining kit. Angiogenesis was analyzed by tube formation assay. The expression of CD31 was analyzed by immunofluorescence. The levels of proteins were measured by western blot assay. The anti-tumor effects of Chrysophanol in vivo were detected by established xenograft mice model. In this study, we found that the cell proliferation, migration, invasion, tube formation, the mitochondrial membrane potential, and the expression of CD31 were inhibited by Chrysophanol in a dose-dependent manner, but cell apoptotic ratios and ROS levels were increased by Chrysophanol in a dose-dependent manner. Furthermore, the effects of Chrysophanol on A549, H738, and HUVEC cell apoptotic rates were reversed by the ROS inhibitor NAC. Besides, the effects of Chrysophanol on HUVEC cell tube formation were reversed by the HIF-1α inhibitor KC7F2 and the VEGF inhibitor axitinib in vitro. Moreover, tumor growth was reduced by Chrysophanol, and the expression of CD31, CD34, and angiogenin was suppressed by Chrysophanol in vivo. Our finding demonstrated that Chrysophanol is a highly effective and low-toxic drug for inhibition of tumor growth especially in high vascularized lung cancer.

  • Chrysophanol exhibits anti cancer activities in lung cancer cell through regulating ros hif 1a vegf signaling pathway
    Naunyn-schmiedebergs Archives of Pharmacology, 2020
    Co-Authors: Jie Zhang, Qian Wang, Qiang Wang, Peng Guo, Yong Wang, Yuqing Xing, Mengmeng Zhang, Fujun Liu, Qingyun Zeng
    Abstract:

    In the present study, we explored the anti-tumor and anti-angiogenesis effects of Chrysophanol, and to investigate the underlying mechanism of the Chrysophanol on anti-tumor and anti-angiogenesis in human lung cancer. The viability of cells was measured by CCK-8 assay, cell apoptosis was measured by Annexin-FITC/PI staining assay, and the cell migration and invasion were analyzed by wound-healing assay and transwell assay. ROS generation and mitochondrial membrane potential were analyzed by DCFH-DA probe and mitochondrial staining kit. Angiogenesis was analyzed by tube formation assay. The expression of CD31 was analyzed by immunofluorescence. The levels of proteins were measured by western blot assay. The anti-tumor effects of Chrysophanol in vivo were detected by established xenograft mice model. In this study, we found that the cell proliferation, migration, invasion, tube formation, the mitochondrial membrane potential, and the expression of CD31 were inhibited by Chrysophanol in a dose-dependent manner, but cell apoptotic ratios and ROS levels were increased by Chrysophanol in a dose-dependent manner. Furthermore, the effects of Chrysophanol on A549, H738, and HUVEC cell apoptotic rates were reversed by the ROS inhibitor NAC. Besides, the effects of Chrysophanol on HUVEC cell tube formation were reversed by the HIF-1α inhibitor KC7F2 and the VEGF inhibitor axitinib in vitro. Moreover, tumor growth was reduced by Chrysophanol, and the expression of CD31, CD34, and angiogenin was suppressed by Chrysophanol in vivo. Our finding demonstrated that Chrysophanol is a highly effective and low-toxic drug for inhibition of tumor growth especially in high vascularized lung cancer.

Markus Riederer - One of the best experts on this subject based on the ideXlab platform.

  • direct effects of physcion Chrysophanol emodin and pachybasin on germination and appressorium formation of the barley hordeum vulgare l powdery mildew fungus blumeria graminis f sp hordei dc speer
    Journal of Agricultural and Food Chemistry, 2018
    Co-Authors: Ulrich Hildebrandt, Alexander Marsell, Markus Riederer
    Abstract:

    Several anthraquinone derivatives are active components of fungicidal formulations particularly effective against powdery mildew fungi. The antimildew effect of compounds such as physcion and Chrysophanol is largely attributed to host plant defense induction. However, so far a direct fungistatic/fungicidal effect of anthraquinone derivatives on powdery mildew fungi has not been unequivocally demonstrated. By applying a Formvar-based in vitro system we demonstrate a direct, dose-dependent effect of physcion, Chrysophanol, emodin, and pachybasin on conidial germination and appressorium formation of Blumeria graminis f. sp. hordei (DC.) Speer, the causative agent of barley (Hordeum vulgare L.) powdery mildew. Physcion was the most effective among the tested compounds. At higher doses, physcion mainly inhibited conidial germination. At lower rates, however, a distinct interference with appressorium formation became discernible. Physcion and others may act by modulating both the infection capacity of the powde...

  • Direct Effects of Physcion, Chrysophanol, Emodin, and Pachybasin on Germination and Appressorium Formation of the Barley (Hordeum vulgare L.) Powdery Mildew Fungus Blumeria graminis f. sp. hordei (DC.) Speer
    2018
    Co-Authors: Ulrich Hildebrandt, Alexander Marsell, Markus Riederer
    Abstract:

    Several anthraquinone derivatives are active components of fungicidal formulations particularly effective against powdery mildew fungi. The antimildew effect of compounds such as physcion and Chrysophanol is largely attributed to host plant defense induction. However, so far a direct fungistatic/fungicidal effect of anthraquinone derivatives on powdery mildew fungi has not been unequivocally demonstrated. By applying a Formvar-based in vitro system we demonstrate a direct, dose-dependent effect of physcion, Chrysophanol, emodin, and pachybasin on conidial germination and appressorium formation of Blumeria graminis f. sp. hordei (DC.) Speer, the causative agent of barley (Hordeum vulgare L.) powdery mildew. Physcion was the most effective among the tested compounds. At higher doses, physcion mainly inhibited conidial germination. At lower rates, however, a distinct interference with appressorium formation became discernible. Physcion and others may act by modulating both the infection capacity of the powdery mildew pathogen and host plant defense. Our results suggest a specific arrangement of substituents at the anthraquinone backbone structure being crucial for the direct antimildew effect

Jie Zhang - One of the best experts on this subject based on the ideXlab platform.

  • retraction note to Chrysophanol exhibits anti cancer activities in lung cancer cell through regulating ros hif 1a vegf signaling pathway
    Naunyn-schmiedebergs Archives of Pharmacology, 2020
    Co-Authors: Jie Zhang, Qian Wang, Qiang Wang, Peng Guo, Yong Wang, Yuqing Xing, Mengmeng Zhang, Fujun Liu, Qingyun Zeng
    Abstract:

    In the present study, we explored the anti-tumor and anti-angiogenesis effects of Chrysophanol, and to investigate the underlying mechanism of the Chrysophanol on anti-tumor and anti-angiogenesis in human lung cancer. The viability of cells was measured by CCK-8 assay, cell apoptosis was measured by Annexin-FITC/PI staining assay, and the cell migration and invasion were analyzed by wound-healing assay and transwell assay. ROS generation and mitochondrial membrane potential were analyzed by DCFH-DA probe and mitochondrial staining kit. Angiogenesis was analyzed by tube formation assay. The expression of CD31 was analyzed by immunofluorescence. The levels of proteins were measured by western blot assay. The anti-tumor effects of Chrysophanol in vivo were detected by established xenograft mice model. In this study, we found that the cell proliferation, migration, invasion, tube formation, the mitochondrial membrane potential, and the expression of CD31 were inhibited by Chrysophanol in a dose-dependent manner, but cell apoptotic ratios and ROS levels were increased by Chrysophanol in a dose-dependent manner. Furthermore, the effects of Chrysophanol on A549, H738, and HUVEC cell apoptotic rates were reversed by the ROS inhibitor NAC. Besides, the effects of Chrysophanol on HUVEC cell tube formation were reversed by the HIF-1α inhibitor KC7F2 and the VEGF inhibitor axitinib in vitro. Moreover, tumor growth was reduced by Chrysophanol, and the expression of CD31, CD34, and angiogenin was suppressed by Chrysophanol in vivo. Our finding demonstrated that Chrysophanol is a highly effective and low-toxic drug for inhibition of tumor growth especially in high vascularized lung cancer.

  • Chrysophanol exhibits anti cancer activities in lung cancer cell through regulating ros hif 1a vegf signaling pathway
    Naunyn-schmiedebergs Archives of Pharmacology, 2020
    Co-Authors: Jie Zhang, Qian Wang, Qiang Wang, Peng Guo, Yong Wang, Yuqing Xing, Mengmeng Zhang, Fujun Liu, Qingyun Zeng
    Abstract:

    In the present study, we explored the anti-tumor and anti-angiogenesis effects of Chrysophanol, and to investigate the underlying mechanism of the Chrysophanol on anti-tumor and anti-angiogenesis in human lung cancer. The viability of cells was measured by CCK-8 assay, cell apoptosis was measured by Annexin-FITC/PI staining assay, and the cell migration and invasion were analyzed by wound-healing assay and transwell assay. ROS generation and mitochondrial membrane potential were analyzed by DCFH-DA probe and mitochondrial staining kit. Angiogenesis was analyzed by tube formation assay. The expression of CD31 was analyzed by immunofluorescence. The levels of proteins were measured by western blot assay. The anti-tumor effects of Chrysophanol in vivo were detected by established xenograft mice model. In this study, we found that the cell proliferation, migration, invasion, tube formation, the mitochondrial membrane potential, and the expression of CD31 were inhibited by Chrysophanol in a dose-dependent manner, but cell apoptotic ratios and ROS levels were increased by Chrysophanol in a dose-dependent manner. Furthermore, the effects of Chrysophanol on A549, H738, and HUVEC cell apoptotic rates were reversed by the ROS inhibitor NAC. Besides, the effects of Chrysophanol on HUVEC cell tube formation were reversed by the HIF-1α inhibitor KC7F2 and the VEGF inhibitor axitinib in vitro. Moreover, tumor growth was reduced by Chrysophanol, and the expression of CD31, CD34, and angiogenin was suppressed by Chrysophanol in vivo. Our finding demonstrated that Chrysophanol is a highly effective and low-toxic drug for inhibition of tumor growth especially in high vascularized lung cancer.

  • Chrysophanol ameliorates high fat diet induced obesity and inflammation in neonatal rats
    Die Pharmazie, 2018
    Co-Authors: Jie Zhang, Hua Kang, Lifang Wang, Xiaoyan Zhao
    Abstract:

    Chrysophanol is a member of the anthraquinone family abundant in rhubarb, a widely used herb for obesity treatment in Traditional Chinese Medicine. Though several studies have indicated numerous features of Chrysophanol, no study has yet reported the effect of Chrysophanol on juvenile obesity. In this study, we tried to identify the anti-obesity effects of Chrysophanol by using high-fat diet (HFD)-induced rats as in vivo models. In HFD rats, Chrysophanol treatment decreased body weight, blood glucose and the blood level of triglyceride (TG), and enhanced the level of high-density lipoprotein-cholesterol (HDL-C). In addition, Chrysophanol markedly reduced lipid accumulation in HFD rats-derived primary hepatocytes. Moreover, Chrysophanol effectively relieved HFD-induced inflammation, as demonstrated by the reduction of interleukin (IL)-6 and IL-1β and the elevation of IL-10. Furthermore, Chrysophanol markedly increased the levels of lipolytic genes and decreased the expressions of lipogenic genes in HFD rats, which was probably benefited from the activation of AMP-activated protein kinase (AMPK)/ Sirtuin 1 (SIRT1). Taken together our study has demonstrated that Chrysophanol could improve the HFD-induced obesity and provided a molecular basis for Chrysophanol potential applications in the treatment of juvenile obesity and other metabolic diseases.

Ulrich Hildebrandt - One of the best experts on this subject based on the ideXlab platform.

  • direct effects of physcion Chrysophanol emodin and pachybasin on germination and appressorium formation of the barley hordeum vulgare l powdery mildew fungus blumeria graminis f sp hordei dc speer
    Journal of Agricultural and Food Chemistry, 2018
    Co-Authors: Ulrich Hildebrandt, Alexander Marsell, Markus Riederer
    Abstract:

    Several anthraquinone derivatives are active components of fungicidal formulations particularly effective against powdery mildew fungi. The antimildew effect of compounds such as physcion and Chrysophanol is largely attributed to host plant defense induction. However, so far a direct fungistatic/fungicidal effect of anthraquinone derivatives on powdery mildew fungi has not been unequivocally demonstrated. By applying a Formvar-based in vitro system we demonstrate a direct, dose-dependent effect of physcion, Chrysophanol, emodin, and pachybasin on conidial germination and appressorium formation of Blumeria graminis f. sp. hordei (DC.) Speer, the causative agent of barley (Hordeum vulgare L.) powdery mildew. Physcion was the most effective among the tested compounds. At higher doses, physcion mainly inhibited conidial germination. At lower rates, however, a distinct interference with appressorium formation became discernible. Physcion and others may act by modulating both the infection capacity of the powde...

  • Direct Effects of Physcion, Chrysophanol, Emodin, and Pachybasin on Germination and Appressorium Formation of the Barley (Hordeum vulgare L.) Powdery Mildew Fungus Blumeria graminis f. sp. hordei (DC.) Speer
    2018
    Co-Authors: Ulrich Hildebrandt, Alexander Marsell, Markus Riederer
    Abstract:

    Several anthraquinone derivatives are active components of fungicidal formulations particularly effective against powdery mildew fungi. The antimildew effect of compounds such as physcion and Chrysophanol is largely attributed to host plant defense induction. However, so far a direct fungistatic/fungicidal effect of anthraquinone derivatives on powdery mildew fungi has not been unequivocally demonstrated. By applying a Formvar-based in vitro system we demonstrate a direct, dose-dependent effect of physcion, Chrysophanol, emodin, and pachybasin on conidial germination and appressorium formation of Blumeria graminis f. sp. hordei (DC.) Speer, the causative agent of barley (Hordeum vulgare L.) powdery mildew. Physcion was the most effective among the tested compounds. At higher doses, physcion mainly inhibited conidial germination. At lower rates, however, a distinct interference with appressorium formation became discernible. Physcion and others may act by modulating both the infection capacity of the powdery mildew pathogen and host plant defense. Our results suggest a specific arrangement of substituents at the anthraquinone backbone structure being crucial for the direct antimildew effect