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Tatjana A. Iezhova - One of the best experts on this subject based on the ideXlab platform.
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Description of Haemoproteus Ciconiae sp. nov. (Haemoproteidae, Haemosporida) from the white stork Ciconia Ciconia, with remarks on insensitivity of established polymerase chain reaction assays to detect this infection
Parasitology Research, 2016Co-Authors: Gediminas Valkiūnas, Mikas Ilgūnas, Dovilė Bukauskaitė, Tatjana A. IezhovaAbstract:Haemoproteus Ciconiae sp. nov. (Haemosporida, Haemoproteidae) was found in the white stork Ciconia Ciconia (Ciconiiformes, Ciconiidae) after spring migration in Lithuania. This organism is illustrated and described based on the morphology of its blood stages. The new species can be readily distinguished from all other haemoproteids parasitizing ciconiiform birds due to the presence of large number (approximately 20 on average) small (
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description of haemoproteus Ciconiae sp nov haemoproteidae haemosporida from the white stork Ciconia Ciconia with remarks on insensitivity of established polymerase chain reaction assays to detect this infection
Parasitology Research, 2016Co-Authors: Gediminas Valkiūnas, Mikas Ilgūnas, Dovilė Bukauskaitė, Tatjana A. IezhovaAbstract:Haemoproteus Ciconiae sp. nov. (Haemosporida, Haemoproteidae) was found in the white stork Ciconia Ciconia (Ciconiiformes, Ciconiidae) after spring migration in Lithuania. This organism is illustrated and described based on the morphology of its blood stages. The new species can be readily distinguished from all other haemoproteids parasitizing ciconiiform birds due to the presence of large number (approximately 20 on average) small (<1 μm) pigment granules in its mature gametocytes. Growing and mature gametocytes of H. Ciconiae were readily visible in all blood films (parasitemia of 0.001 %). However, experienced researchers were unable to detect sequences of its mitochondrial cytochrome b (cyt b) or apicoplast genes from the microscopically positive sample by using five established assays for polymerase chain reaction (PCR)-based detection of avian haemosporidian parasites. The white stork cyt b sequence was readily detectable, indicating the well-optimised PCR protocols and the good quality of total DNA in the sample containing the new species. The failure to amplify this parasite DNA indicates insufficient sensitivity of the currently used PCR-based assays in diagnostics of avian haemosporidian infections. We suggest possible explanations of this observation. To minimize number of the false negative PCR reports, we call for the continued use of optical microscopy in parallel with molecular diagnostics in studies of haemosporidian parasites, particularly in wildlife.
Gediminas Valkiūnas - One of the best experts on this subject based on the ideXlab platform.
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Description of Haemoproteus Ciconiae sp. nov. (Haemoproteidae, Haemosporida) from the white stork Ciconia Ciconia, with remarks on insensitivity of established polymerase chain reaction assays to detect this infection
Parasitology Research, 2016Co-Authors: Gediminas Valkiūnas, Mikas Ilgūnas, Dovilė Bukauskaitė, Tatjana A. IezhovaAbstract:Haemoproteus Ciconiae sp. nov. (Haemosporida, Haemoproteidae) was found in the white stork Ciconia Ciconia (Ciconiiformes, Ciconiidae) after spring migration in Lithuania. This organism is illustrated and described based on the morphology of its blood stages. The new species can be readily distinguished from all other haemoproteids parasitizing ciconiiform birds due to the presence of large number (approximately 20 on average) small (
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description of haemoproteus Ciconiae sp nov haemoproteidae haemosporida from the white stork Ciconia Ciconia with remarks on insensitivity of established polymerase chain reaction assays to detect this infection
Parasitology Research, 2016Co-Authors: Gediminas Valkiūnas, Mikas Ilgūnas, Dovilė Bukauskaitė, Tatjana A. IezhovaAbstract:Haemoproteus Ciconiae sp. nov. (Haemosporida, Haemoproteidae) was found in the white stork Ciconia Ciconia (Ciconiiformes, Ciconiidae) after spring migration in Lithuania. This organism is illustrated and described based on the morphology of its blood stages. The new species can be readily distinguished from all other haemoproteids parasitizing ciconiiform birds due to the presence of large number (approximately 20 on average) small (<1 μm) pigment granules in its mature gametocytes. Growing and mature gametocytes of H. Ciconiae were readily visible in all blood films (parasitemia of 0.001 %). However, experienced researchers were unable to detect sequences of its mitochondrial cytochrome b (cyt b) or apicoplast genes from the microscopically positive sample by using five established assays for polymerase chain reaction (PCR)-based detection of avian haemosporidian parasites. The white stork cyt b sequence was readily detectable, indicating the well-optimised PCR protocols and the good quality of total DNA in the sample containing the new species. The failure to amplify this parasite DNA indicates insufficient sensitivity of the currently used PCR-based assays in diagnostics of avian haemosporidian infections. We suggest possible explanations of this observation. To minimize number of the false negative PCR reports, we call for the continued use of optical microscopy in parallel with molecular diagnostics in studies of haemosporidian parasites, particularly in wildlife.
Michael Lierz - One of the best experts on this subject based on the ideXlab platform.
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description and prevalence of mycoplasma Ciconiae sp nov isolated from white stork nestlings Ciconia Ciconia
International Journal of Systematic and Evolutionary Microbiology, 2016Co-Authors: Franca Moller Palauribes, Hafez M Hafez, Dirk Enderlein, N Hagen, Werner Herbst, Michael LierzAbstract:The mycoplasma strain ST 57T was isolated from the trachea of a clinically healthy, free-ranging white stork nestling in Nielitz, Mecklenburg-Western Pomerania, Germany. Strain ST 57T grew in fried-egg-shaped colonies on mycoplasma (SP4) agar plates and was dependent on sterol for growth. The organism fermented glucose and did not hydrolyse arginine or urea. The optimal growth temperature was 37 °C, with a temperature range from 23 to 44 °C. Strain ST 57Tcould not be identified as a representative of any of the currently described mycoplasma species by alignment of the 16S rRNA gene sequence or 16S–23S intergenic transcribed spacer region, or by immunobinding assays. Thus, this organism appears to be a representative of a novel species, for which the name Mycoplasma Ciconiae sp. nov. is proposed. The type strain is ST 57T (=ATCC BAA-2401T=DSM 25251T). Four further strains of this species are included in this description (ST 24=DSM 29908, ST 56 Clone 1=DSM 29054, ST 99=DSM 29909, ST 102=DSM 29010). The prevalence of this mycoplasma species in clinically healthy, white stork nestlings in northern Germany was determined. Our species-specific PCR detected 57.8 % (48/83) of the samples positive for M. Ciconiae sp. nov. As this species appears to be widespread in the healthy free-ranging white stork population, we conclude that this species is either apathogenic or an opportunistic pathogen in white storks.
Ulrich Querner - One of the best experts on this subject based on the ideXlab platform.
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conservation measures based on migration research in white storks Ciconia Ciconia Ciconia boyciana
Acta Zoologica Sinica, 2006Co-Authors: Peter Berthold, Michael Kaatz, W Van Den Bossche, Ulrich QuernerAbstract:For over a decade, we have been using satellite tracking to study the migration of white storks (Ciconia Ciconia) along their eastern migration route from Europe to southern Africa. About 120 individuals have been monitored by satellite-tracking to determine the route of the journey, choice of staging areas and overwintering patterns. Satellite-tracking not only confirmed migration routes formerly elucidated by ringing but also enabled us to detect new routes to western Sudan and Chad and identify large staging areas in this region. The most important results of the tracking are the identification of 15 major staging areas during migration and wintering, for which specific conservation measures are being implemented in a "stepping-stone" project in accord with the Bonn Convention. We have also investigated the migration physiology and stopover-site ecology of the storks. Because storks migrate to northeastern Africa with minimal fat stores and very low food intake, resting areas on this sector of their route, are currently in greater need of protection than feeding grounds. In a second, smaller study, oriental white storks (Ciconia boyciana) are being tracked from Siberia to locate their overwintering grounds in China, and identify and implement appropriate conservation measures for this species.
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long term satellite tracking of white stork Ciconia Ciconia migration constancy versus variability
Journal of Ornithology, 2004Co-Authors: Peter Berthold, Michael Kaatz, Ulrich QuernerAbstract:During satellite-tracking investigations of 140 white storks (Ciconia Ciconia), one individual, the long-term world record holder among birds, was followed along 12 migratory journeys over a period of 10 years. This long-term study confirms what previous several-year tracking studies of white storks had indicated: there can be great variability from year to year in the choice of winter quarters as well as in the routes and times of migration, intermediate destinations and stop-over periods, but constancy of winter quarters and migration routes is also possible. The variability may well be caused by external factors, of which food supply is probably predominant.
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detection of a new important staging and wintering area of the white stork Ciconia Ciconia by satellite tracking
Ibis, 2001Co-Authors: Peter Berthold, Michael Kaatz, Willem Van Den Bossche, Christoph Kaatz, Eugeniusz Nowak, Yossi Leshem, Wolfgang Fiedler, Ulrich QuernerAbstract:Since 1991, a large-scale satellite traclung study of White Stork Ciconia Ciconia has followed 75 individuals along the eastern migration route, which passes across Israel into eastern Africa. Twenty-six of these birds travelled at least as far as the Sudan. Fifteen (58%) did not migrate to the eastern Sudan - the primary winter quarters in Africa suggested by ring recoveries - but flew to western Sudan and Chad and in one case even through Cameroon and into Nigeria. The significance of this new, important staging region for White Storks should be investigated urgently, to establish the areas that are ecologically valuable for the conservation of this species. The various European populations of the White Stork Ciconia Ciconia are known to overwinter mainly in Africa and to reach this continent by routes that largely bypass the Mediterranean Sea. There are two such routes, separated by a ‘migration divide’: the western populations fly over the Iberian Peninsula and Gibraltar, primarily to northwestern Africa, whereas the eastern populations pass over Israel and the Sinai Peninsula into eastern Africa, some as far as the southern tip of the continent. For the eastern migrants, the Sudan is an important stopping place during winter. Some Storks rest there briefly, others use it as an intermediate destination for longer sojourns, and for still others it is the final winter quarters (Schuz et al. 1971). The recoveries made here in almost 100 years of White Stork ringing (Jespersen & Thing 1950) and many visual observations made on expeditions have suggested that the eastern Sudan is the most important region for White Storks.
Mikas Ilgūnas - One of the best experts on this subject based on the ideXlab platform.
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Description of Haemoproteus Ciconiae sp. nov. (Haemoproteidae, Haemosporida) from the white stork Ciconia Ciconia, with remarks on insensitivity of established polymerase chain reaction assays to detect this infection
Parasitology Research, 2016Co-Authors: Gediminas Valkiūnas, Mikas Ilgūnas, Dovilė Bukauskaitė, Tatjana A. IezhovaAbstract:Haemoproteus Ciconiae sp. nov. (Haemosporida, Haemoproteidae) was found in the white stork Ciconia Ciconia (Ciconiiformes, Ciconiidae) after spring migration in Lithuania. This organism is illustrated and described based on the morphology of its blood stages. The new species can be readily distinguished from all other haemoproteids parasitizing ciconiiform birds due to the presence of large number (approximately 20 on average) small (
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description of haemoproteus Ciconiae sp nov haemoproteidae haemosporida from the white stork Ciconia Ciconia with remarks on insensitivity of established polymerase chain reaction assays to detect this infection
Parasitology Research, 2016Co-Authors: Gediminas Valkiūnas, Mikas Ilgūnas, Dovilė Bukauskaitė, Tatjana A. IezhovaAbstract:Haemoproteus Ciconiae sp. nov. (Haemosporida, Haemoproteidae) was found in the white stork Ciconia Ciconia (Ciconiiformes, Ciconiidae) after spring migration in Lithuania. This organism is illustrated and described based on the morphology of its blood stages. The new species can be readily distinguished from all other haemoproteids parasitizing ciconiiform birds due to the presence of large number (approximately 20 on average) small (<1 μm) pigment granules in its mature gametocytes. Growing and mature gametocytes of H. Ciconiae were readily visible in all blood films (parasitemia of 0.001 %). However, experienced researchers were unable to detect sequences of its mitochondrial cytochrome b (cyt b) or apicoplast genes from the microscopically positive sample by using five established assays for polymerase chain reaction (PCR)-based detection of avian haemosporidian parasites. The white stork cyt b sequence was readily detectable, indicating the well-optimised PCR protocols and the good quality of total DNA in the sample containing the new species. The failure to amplify this parasite DNA indicates insufficient sensitivity of the currently used PCR-based assays in diagnostics of avian haemosporidian infections. We suggest possible explanations of this observation. To minimize number of the false negative PCR reports, we call for the continued use of optical microscopy in parallel with molecular diagnostics in studies of haemosporidian parasites, particularly in wildlife.