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B. Prathibha Devi - One of the best experts on this subject based on the ideXlab platform.
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In vitro Propagation of Clitoria ternatea L., A Valuable Medicinal Plant
Trends in Biosciences, 2014Co-Authors: Ch. Mohan, P. Manjula, B. Kiran Kumar, B. Naresh, B. Ramadevi, B. Prathibha DeviAbstract:Tissue culture studies of Clitoria ternatea L. is reported here which is commonly known as ‘butterfly pea’ and “shankhapushpi”. It is a traditional Ayurvedic medicinal plant belonging to the family Fabaceae. The drug ‘shankhapushpum’ of Ayurveda consists of the roots and seeds of Clitoria ternatea L. and is used as a ‘tonic of the nerves’, alternative and laxative. The leaves and roots are used in the treatment. In vitro propagation is an alternative method of propagation which can aid its conservation. The explants are cultured on MS medium containing different concentration and combinations of growth regulators like 6-benzylaminopurine (BAP). Multiple shoot buds were regenerated successfully from the nodal explants and efficiently rooted on half strength MS medium supplemented with IBA. The regenerated plantlets were successfully transferred to the glasshouse, acclimatized and transferred to the field.
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Phytochemical analysis of Clitoria ternatea Linn., a valuable medicinal plant
The Journal of Indian Botanical Society, 2013Co-Authors: P. Manjula, Ch. Mohan, D. Sreekanth, B. Keerthi, B. Prathibha DeviAbstract:The present paper deals with phytochemical studies in Clitoria ternatea Linn.. It is commonly known as ‘butterfly pea ’and “shankhapushpi”. It is a traditional Ayurvedic medicinal plant belonging to the family Fabaceae. The plant extracts were subjected to phytochemical analysis for screening of medicinal constituents. Valuable data has been collected pertaining to the presence of various phytochemicals like Alkaloids, Tannins, Glycosides, Resins, Steroids, Saponins, Flavonoids and Phenols. Further, quantitative estimation of total Flavonoids, Saponins and Phenols was also carried out which has provided information regarding the medicinal potential of the plant.
Ravindra B. Malabadi - One of the best experts on this subject based on the ideXlab platform.
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Antibacterial activity of silver nanoparticles synthesized by using whole plant extracts of Clitoria ternatea
2015Co-Authors: Ravindra B. Malabadi, Gangadhar S. Mulgund, Neelambika T. Meti, Karaba N. Nataraja, S. Vijaya KumarAbstract:This study highlights the synthesis of silver nanoparticles using whole plant extracts of Clitoria ternatea. Antibacterial activity of silver nanoparticles was assessed by using disc diffusion method against Bacillus subtilis, Staphylococcus aureus, Escherichia coli and Klebsiella pneumoniae, since Bacillus species and S. aureus strains may cause diarrhoea and an enteropathogenic form of E. coli, and Klebsiella species may cause food poising. The results of this study clearly indicate that silver nanoparticles synthesized from plant extracts of Clitoria ternatea has many pharmaceutical applications for the control of deadly pathogens.
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Regular Article Antibacterial activity of silver nanoparticles synthesized by using whole plant extracts of Clitoria ternatea
2012Co-Authors: Ravindra B. Malabadi, Gangadhar S. Mulgund, Neelambika T. Meti, Karaba N. Nataraja, S. Vijaya KumarAbstract:This study highlights the synthesis of silver nanoparticles using whole plant extracts of Clitoria ternatea. Antibacterial activity of silver nanoparticles was assessed by using disc diffusion method against Bacillus subtilis, Staphylococcus aureus, Escherichia coli and Klebsiella pneumoniae, since Bacillus species and S. aureus strains may cause diarrhoea and an enteropathogenic form of E. coli, and Klebsiella species may cause food poising. The results of this study clearly indicate that silver nanoparticles synthesized from plant extracts of Clitoria ternatea has many pharmaceutical applications for the control of deadly pathogens.
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In vitro storage of synthetic seeds in Clitoria ternatea Linn.
Phytomorphology, 2002Co-Authors: Ravindra B. Malabadi, K. NatarajaAbstract:Somatic embryos of Clitoria ternatea were encapsulated with 2.5% (w/v) sodium alginate and incubated for 5 min with CaCl 2 (100mM). Encapsulated (Synthetic) and nonencapsulated embryos were stored in dark at 2°, 10°, 20° and 27°C for up to 90 days. Synthetic seeds could be stored at 2°C for 50 days without reduction in viability as compared with nonencapsulated somatic embryos which showed 4% viability after 30 days at 2°C.
S. Vijaya Kumar - One of the best experts on this subject based on the ideXlab platform.
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Antibacterial activity of silver nanoparticles synthesized by using whole plant extracts of Clitoria ternatea
2015Co-Authors: Ravindra B. Malabadi, Gangadhar S. Mulgund, Neelambika T. Meti, Karaba N. Nataraja, S. Vijaya KumarAbstract:This study highlights the synthesis of silver nanoparticles using whole plant extracts of Clitoria ternatea. Antibacterial activity of silver nanoparticles was assessed by using disc diffusion method against Bacillus subtilis, Staphylococcus aureus, Escherichia coli and Klebsiella pneumoniae, since Bacillus species and S. aureus strains may cause diarrhoea and an enteropathogenic form of E. coli, and Klebsiella species may cause food poising. The results of this study clearly indicate that silver nanoparticles synthesized from plant extracts of Clitoria ternatea has many pharmaceutical applications for the control of deadly pathogens.
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Regular Article Antibacterial activity of silver nanoparticles synthesized by using whole plant extracts of Clitoria ternatea
2012Co-Authors: Ravindra B. Malabadi, Gangadhar S. Mulgund, Neelambika T. Meti, Karaba N. Nataraja, S. Vijaya KumarAbstract:This study highlights the synthesis of silver nanoparticles using whole plant extracts of Clitoria ternatea. Antibacterial activity of silver nanoparticles was assessed by using disc diffusion method against Bacillus subtilis, Staphylococcus aureus, Escherichia coli and Klebsiella pneumoniae, since Bacillus species and S. aureus strains may cause diarrhoea and an enteropathogenic form of E. coli, and Klebsiella species may cause food poising. The results of this study clearly indicate that silver nanoparticles synthesized from plant extracts of Clitoria ternatea has many pharmaceutical applications for the control of deadly pathogens.
Tri Putriany Agustin - One of the best experts on this subject based on the ideXlab platform.
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Antibiofilm Effect of Clitoria ternatea Flower Juice on Porphyromonas gingivalis in vitro
Journal of Indonesian Dental Association, 2018Co-Authors: Armelia Sari Widyarman, Stephani Sumadi, Tri Putriany AgustinAbstract:Introduction: Clitoria ternatea flower contains flavonoid such as anthocyanin that gives the blue color to its flower and has antimicrobial activity. Objectives: The aim of this study was to examine the effect of flower juice of Clitoria ternatea against Porphyromonas gingivalis biofilm viability in vitro. Methods: This study was experimental laboratory research using biofilm assay method. P. gingivalis was cultured in BHI broth in 37°C for 24h under anaerobic condition. Fresh flowers of Clitoria ternatea were extracted using mortar and pestle and diluted into 6 different concentrations: 100%, 50%, 25%, 12.5%, 6.25%, and 3.125% with phosphate buffer saline (PBS). Chlorhexidine (0.2%) was used as positive control and biofilm without treatment as negative control. The flower juice was distributed into 96 well-plates that contained biofilm of P. gingivalis and incubated for 1h, 3h, 6h, and 24h in 37°C, anaerobic atmosphere. Biofilm was measured using crystal violet dye with microplate reader (490 nm). Data were statistically analysed using one-way ANOVA test and Post Hoc test with p<0.05 was set as significant different. Result: Result showed that Clitoria ternatea flower juice significantly reduced the Porphyromonas gingivalis biofilm viability in all concentration and all incubation time. The most effective concentration to inhibit Porphyromonas gingivalis biofilm was 100% in 1h incubation time which biofilm was diminished (Optical Density=0.00). One way ANOVA test and Post Hoc test showed a significant biofilm reduction in all concentration and all incubation time after treatment with the flower juice compared to control (p<0.05). Conclusion: Clitoria ternatea flower juice has antibiofilm effect against Porphyromonas gingivalis. This result showed this flower juice may be useful for combating periodontal pathogens. However, further studies using other bacteria are still needed to confirm this result.
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Antibiofilm Effect of Clitoria ternatea Flower Juice on Porphyromonas gingivalis in vitro
Journal of Indonesian Dental Association, 2018Co-Authors: Armelia Sari Widyarman, Stephani Sumadi, Tri Putriany AgustinAbstract:Introduction: Clitoria ternatea flower contains flavonoid such as anthocyanin that gives the blue color to its flower and has antimicrobial activity. Objectives: The aim of this study was to examine the effect of flower juice of Clitoria ternatea against Porphyromonas gingivalis biofilm viability in vitro. Methods: This study was experimental laboratory research using biofilm assay method. P. gingivalis was cultured in BHI broth in 37°C for 24h under anaerobic condition. Fresh flowers of Clitoria ternatea were extracted using mortar and pestle and diluted into 6 different concentrations: 100%, 50%, 25%, 12.5%, 6.25%, and 3.125% with phosphate buffer saline (PBS). Chlorhexidine (0.2%) was used as positive control and biofilm without treatment as negative control. The flower juice was distributed into 96 well-plates that contained biofilm of P. gingivalis and incubated for 1h, 3h, 6h, and 24h in 37°C, anaerobic atmosphere. Biofilm was measured using crystal violet dye with microplate reader (490 nm). Data were statistically analysed using one-way ANOVA test and Post Hoc test with p
Wee Sim Choo - One of the best experts on this subject based on the ideXlab platform.
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Extraction methods of butterfly pea (Clitoria ternatea) flower and biological activities of its phytochemicals
Journal of Food Science and Technology, 2020Co-Authors: Ethel Jeyaseela Jeyaraj, Yau Yan Lim, Wee Sim ChooAbstract:Clitoria ternatea or commonly known as ‘Butterfly pea’ has been used traditionally in Ayurvedic medicine in which various parts of the plants are used to treat health issues such as indigestion, constipation, arthritis, skin diseases, liver and intestinal problems. The flowers of C. ternatea are used worldwide as ornamental flowers and traditionally used as a food colorant. This paper reviews the recent advances in the extraction and biological activities of phytochemicals from C. ternatea flowers. The application of maceration or ultrasound assisted extraction greatly increased the yield (16–247% of increase) of phytochemicals from C. ternatea flowers. Various phytochemicals such as kaempferol, quercetin and myricetin glycosides as well as anthocyanins have been isolated from C. ternatea flowers. Clitoria ternatea flower extracts were found to possess antimicrobial, antioxidant, anti-inflammatory, cytotoxic and antidiabetic activities which are beneficial to human health. Clitoria ternatea flower is a promising candidate for functional food applications owing to its wide range of pharmacotherapeutic properties as well as its safety and effectiveness.