The Experts below are selected from a list of 9225 Experts worldwide ranked by ideXlab platform
Yitao Zeng - One of the best experts on this subject based on the ideXlab platform.
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association between mitochondrial dna haplotype compatibility and increased efficiency of bovine intersubspecies cloning
2011Co-Authors: Hao Yan, Zhonghai Yan, Fei Jiao, Shuzhen Huang, Fanyi Zeng, Yitao ZengAbstract:Reconstructed embryos derived from intersubspecies somatic cell nuclear transfer (SCNT) have poorer developmental potential than those from intrasubspecies SCNT. Based on our previous study that Holstein dairy bovine (HD) mitochondrial DNA (mtDNA) haplotype compatibility between donor karyoplast and recipient cytoplast is crucial for SCNT embryo development, we performed intersubspecies SCNT using HD as donor karyoplast and Luxi yellow heifer (LY) as recipient cytoplast according to mtDNA haplotypes determined by polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) analysis. The results demonstrated that intersubspecies mtDNA homotype SCNT embryos had higher pre- and post-implantation developmental competence than intrasubspecies mtDNA heterotype embryos as well as improved blastocyst reprogramming status, including normal H3K9 dimethylation pattern and promoter hypomethylation of pluripotent genes such as Oct4 and Sox2, suggesting that intersubspecies SCNT using LY oocytes maintains HD cloning efficiency and may reprogram HD nuclei to develop into a normal Cloned Animal ultimately. Our results indicated that karyoplast-cytoplast interactions and mtDNA haplotype compatibility may affect bovine intersubspecies SCNT efficiency. This study on bovine intersubspecies SCNT is valuable for understanding the mechanisms of mtDNA haplotype compatibility between karyoplast and cytoplast impacting the bovine SCNT efficiency, and provides an alternative and economic resource for HD cloning.
Guangpeng Li - One of the best experts on this subject based on the ideXlab platform.
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coexpression analysis identifies nuclear reprogramming barriers of somatic cell nuclear transfer embryos
2017Co-Authors: Guanghua Su, Lei Cheng, Yu Feng, Guangpeng LiAbstract:// Yongchun Zuo 1, 2, * , Guanghua Su 1, * , Lei Cheng 1 , Kun Liu 1 , Yu Feng 1 , Zhuying Wei 1 , Chunling Bai 1 , Guifang Cao 2 and Guangpeng Li 1 1 The Research Center for Laboratory Animal Science, College of Life Sciences, Inner Mongolia University, Hohhot 010021, China 2 College of Veterinary Medicine, Inner Mongolia Agricultural University, Hohhot 010018, China * These authors have contributed equally to this work Correspondence to: Yongchun Zuo, email: yczuo@imu.edu.cn Guangpeng Li, email: gpengli@imu.edu.cn Keywords: somatic cell nuclear transfer (SCNT), gene co-expression analysis, enrichment of GO category, pathway aberrant activation, reprogramming barriers Received: April 23, 2017 Accepted: June 30, 2017 Published: July 22, 2017 ABSTRACT The success of Cloned Animal “Dolly Sheep” demonstrated the somatic cell nuclear transfer (SCNT) technique holds huge potentials for mammalian asexual reproduction. However, the extremely poor development of SCNT embryos indicates their molecular mechanism remain largely unexplored. Deciphering the spatiotemporal patterns of gene expression in SCNT embryos is a crucial step toward understanding the mechanisms associated with nuclear reprogramming. In this study, a valuable transcriptome recourse of SCNT embryos was firstly established, which derived from different inter-/intra donor cells. The gene co-expression analysis identified 26 cell-specific modules, and a series of regulatory pathways related to reprogramming barriers were further enriched. Compared to the intra-SCNT embryos, the inter-SCNT embryos underwent only complete partially reprogramming. As master genome trigger genes, the transcripts related to TFIID subunit, RNA polymerase and mediators were incomplete activated in inter-SCNT embryos. The inter-SCNT embryos only wasted the stored maternal mRNA of master regulators, but failed to activate their self-sustained pathway of RNA polymerases. The KDM family of epigenetic regulator also seriously delayed in inter-SCNT embryo reprogramming process. Our study provided new insight into understanding of the mechanisms of nuclear reprogramming.
Naresh L Selokar - One of the best experts on this subject based on the ideXlab platform.
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10 years after the birth of india s first Cloned farm Animal where is buffalo cloning heading
2020Co-Authors: Naresh L SelokarAbstract:India owns the best buffalo breeds, particularly Murrah which is famous all over the world for high milk production. India’s white and pink revolution cannot be imagined without the contribution of buffalo, and to achieve these, the best productive Animals need to be produced through scientific interventions. Animal cloning is a technique used to produce multiple copies of the best Animals without normal reproduction. In India, buffalo cloning has already happened and India’s first Cloned buffalo was produced in 2009. Later, several buffalo clones were produced and attempts are ongoing to produce stock of more elite Animals. Buffalo cloning has made its way from scientific manuscript to farmer’s farm. In this viewpoint article, we provide an overview of the progress of buffalo cloning and we discuss some of the public perceptions of Animal cloning such as aging and food safety of Cloned Animal products
Yadav, Prem Singh - One of the best experts on this subject based on the ideXlab platform.
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10 years after the birth of India’s first Cloned farm Animal, where is buffalo cloning heading
2019Co-Authors: Selokar, Naresh L, Saini Monika, Kumar Dharmendra, Sharma, Rakesh K, Yadav, Prem SinghAbstract:191-192India owns the best buffalo breeds, particularly Murrah which is famous all over the world for high milk production. India’s white and pink revolution cannot be imagined without the contribution of buffalo and to achieve these, the best productive Animals need to be produced through scientific interventions. Animal cloning is a technique used to produce multiple copies of the best Animals without normal reproduction. In India, buffalo cloning has already happened and India’s first Cloned buffalo was produced in 2009. Later, several buffalo clones were produced and attempts are ongoing to produce stock of more elite Animals. Buffalo cloning has made its way from scientific curiosity to farmer’s farm. In this viewpoint article, we provide an overview of the progress of buffalo cloning and we discuss some of the public perceptions of Animal cloning such as aging and food safety of Cloned Animal products
Guanghua Su - One of the best experts on this subject based on the ideXlab platform.
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coexpression analysis identifies nuclear reprogramming barriers of somatic cell nuclear transfer embryos
2017Co-Authors: Guanghua Su, Lei Cheng, Yu Feng, Guangpeng LiAbstract:// Yongchun Zuo 1, 2, * , Guanghua Su 1, * , Lei Cheng 1 , Kun Liu 1 , Yu Feng 1 , Zhuying Wei 1 , Chunling Bai 1 , Guifang Cao 2 and Guangpeng Li 1 1 The Research Center for Laboratory Animal Science, College of Life Sciences, Inner Mongolia University, Hohhot 010021, China 2 College of Veterinary Medicine, Inner Mongolia Agricultural University, Hohhot 010018, China * These authors have contributed equally to this work Correspondence to: Yongchun Zuo, email: yczuo@imu.edu.cn Guangpeng Li, email: gpengli@imu.edu.cn Keywords: somatic cell nuclear transfer (SCNT), gene co-expression analysis, enrichment of GO category, pathway aberrant activation, reprogramming barriers Received: April 23, 2017 Accepted: June 30, 2017 Published: July 22, 2017 ABSTRACT The success of Cloned Animal “Dolly Sheep” demonstrated the somatic cell nuclear transfer (SCNT) technique holds huge potentials for mammalian asexual reproduction. However, the extremely poor development of SCNT embryos indicates their molecular mechanism remain largely unexplored. Deciphering the spatiotemporal patterns of gene expression in SCNT embryos is a crucial step toward understanding the mechanisms associated with nuclear reprogramming. In this study, a valuable transcriptome recourse of SCNT embryos was firstly established, which derived from different inter-/intra donor cells. The gene co-expression analysis identified 26 cell-specific modules, and a series of regulatory pathways related to reprogramming barriers were further enriched. Compared to the intra-SCNT embryos, the inter-SCNT embryos underwent only complete partially reprogramming. As master genome trigger genes, the transcripts related to TFIID subunit, RNA polymerase and mediators were incomplete activated in inter-SCNT embryos. The inter-SCNT embryos only wasted the stored maternal mRNA of master regulators, but failed to activate their self-sustained pathway of RNA polymerases. The KDM family of epigenetic regulator also seriously delayed in inter-SCNT embryo reprogramming process. Our study provided new insight into understanding of the mechanisms of nuclear reprogramming.