The Experts below are selected from a list of 61095 Experts worldwide ranked by ideXlab platform
Davide Sciuscio - One of the best experts on this subject based on the ideXlab platform.
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toward refinement of the colony forming unit granulocyte macrophage Clonogenic assay inclusion of a metabolic system
Toxicology in Vitro, 2006Co-Authors: Gianni Dal Negro, Luca Vandin, Monica Bonato, Davide SciuscioAbstract:This work represents a first attempt to refine the colony-forming unit-granulocyte/macrophage (CFU-GM) Clonogenic assay by incorporating liver microsomes and co-factors as a metabolic system into the in vitro test system in response to an ECVAM recommendation. From the comparison of results obtained with the CFU-GM Clonogenic assay currently used and with the new experimental protocol, different toxicity on granulocyte/macrophage precursors was demonstrated, when drugs with a known metabolism in vivo were tested.
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Toward refinement of the colony-forming unit-granulocyte/macrophage Clonogenic assay: inclusion of a metabolic system.
Toxicology in Vitro, 2005Co-Authors: Gianni Dal Negro, Luca Vandin, Monica Bonato, Davide SciuscioAbstract:This work represents a first attempt to refine the colony-forming unit-granulocyte/macrophage (CFU-GM) Clonogenic assay by incorporating liver microsomes and co-factors as a metabolic system into the in vitro test system in response to an ECVAM recommendation. From the comparison of results obtained with the CFU-GM Clonogenic assay currently used and with the new experimental protocol, different toxicity on granulocyte/macrophage precursors was demonstrated, when drugs with a known metabolism in vivo were tested.
Gianni Dal Negro - One of the best experts on this subject based on the ideXlab platform.
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toward refinement of the colony forming unit granulocyte macrophage Clonogenic assay inclusion of a metabolic system
Toxicology in Vitro, 2006Co-Authors: Gianni Dal Negro, Luca Vandin, Monica Bonato, Davide SciuscioAbstract:This work represents a first attempt to refine the colony-forming unit-granulocyte/macrophage (CFU-GM) Clonogenic assay by incorporating liver microsomes and co-factors as a metabolic system into the in vitro test system in response to an ECVAM recommendation. From the comparison of results obtained with the CFU-GM Clonogenic assay currently used and with the new experimental protocol, different toxicity on granulocyte/macrophage precursors was demonstrated, when drugs with a known metabolism in vivo were tested.
I. W. Blau - One of the best experts on this subject based on the ideXlab platform.
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Bendamustine, but not fludarabine, exhibits a low stem cell toxicity in vitro
Journal of Cancer Research and Clinical Oncology, 2009Co-Authors: M. Schmidt-hieber, A. Busse, B. Reufi, W. Knauf, E. Thiel, I. W. BlauAbstract:Purpose We investigated the in vitro toxicity of bendamustine and fludarabine to hematopoietic progenitors and stem cells from healthy donors. Methods Clonogenic agar colony assays, non-Clonogenic long-term liquid cultures (LTC) and apoptosis assays were used to assess the cytotoxicity of both the agents. Results Total colony-forming units (CFU) were more sensitive to fludarabine than to bendamustine in agar colony assays (IC_50 0.7 μM/L and 8.5 μM/L, respectively). Using the Bliss independence model and combining the two agents yielded additive inhibition of progenitors. Non-Clonogenic assays, including LTC and an apoptosis assay detecting activated caspases showed that stem cells are characterized by low sensitivity to bendamustine. In contrast, fludarabine strongly inhibited the viability and growth of stem cells in LTC. Conclusions Our data show that bendamustine is characterized by lower in vitro toxicity to hematopoietic progenitors and stem cells than fludarabine and might thus be preferable in regimens prior to stem cells apheresis.
Monica Bonato - One of the best experts on this subject based on the ideXlab platform.
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toward refinement of the colony forming unit granulocyte macrophage Clonogenic assay inclusion of a metabolic system
Toxicology in Vitro, 2006Co-Authors: Gianni Dal Negro, Luca Vandin, Monica Bonato, Davide SciuscioAbstract:This work represents a first attempt to refine the colony-forming unit-granulocyte/macrophage (CFU-GM) Clonogenic assay by incorporating liver microsomes and co-factors as a metabolic system into the in vitro test system in response to an ECVAM recommendation. From the comparison of results obtained with the CFU-GM Clonogenic assay currently used and with the new experimental protocol, different toxicity on granulocyte/macrophage precursors was demonstrated, when drugs with a known metabolism in vivo were tested.
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Toward refinement of the colony-forming unit-granulocyte/macrophage Clonogenic assay: inclusion of a metabolic system.
Toxicology in Vitro, 2005Co-Authors: Gianni Dal Negro, Luca Vandin, Monica Bonato, Davide SciuscioAbstract:This work represents a first attempt to refine the colony-forming unit-granulocyte/macrophage (CFU-GM) Clonogenic assay by incorporating liver microsomes and co-factors as a metabolic system into the in vitro test system in response to an ECVAM recommendation. From the comparison of results obtained with the CFU-GM Clonogenic assay currently used and with the new experimental protocol, different toxicity on granulocyte/macrophage precursors was demonstrated, when drugs with a known metabolism in vivo were tested.
Luca Vandin - One of the best experts on this subject based on the ideXlab platform.
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toward refinement of the colony forming unit granulocyte macrophage Clonogenic assay inclusion of a metabolic system
Toxicology in Vitro, 2006Co-Authors: Gianni Dal Negro, Luca Vandin, Monica Bonato, Davide SciuscioAbstract:This work represents a first attempt to refine the colony-forming unit-granulocyte/macrophage (CFU-GM) Clonogenic assay by incorporating liver microsomes and co-factors as a metabolic system into the in vitro test system in response to an ECVAM recommendation. From the comparison of results obtained with the CFU-GM Clonogenic assay currently used and with the new experimental protocol, different toxicity on granulocyte/macrophage precursors was demonstrated, when drugs with a known metabolism in vivo were tested.
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Toward refinement of the colony-forming unit-granulocyte/macrophage Clonogenic assay: inclusion of a metabolic system.
Toxicology in Vitro, 2005Co-Authors: Gianni Dal Negro, Luca Vandin, Monica Bonato, Davide SciuscioAbstract:This work represents a first attempt to refine the colony-forming unit-granulocyte/macrophage (CFU-GM) Clonogenic assay by incorporating liver microsomes and co-factors as a metabolic system into the in vitro test system in response to an ECVAM recommendation. From the comparison of results obtained with the CFU-GM Clonogenic assay currently used and with the new experimental protocol, different toxicity on granulocyte/macrophage precursors was demonstrated, when drugs with a known metabolism in vivo were tested.