The Experts below are selected from a list of 294 Experts worldwide ranked by ideXlab platform
Zhou Shao-en - One of the best experts on this subject based on the ideXlab platform.
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Investigation on the Clonorchis sinensis Infection in Zhaoqing City
Journal of Tropical Medicine, 2007Co-Authors: Zhou Shao-enAbstract:Objective To understand the current status of the Clonorchis sinensis infection in Zhaoqing city and to provide scientific basis for the future control work.Method The sampling of individuals was done by the lamination random sampling.Clonorchis sinensis eggs in stool were examined by the improved Kato-Katz’s thick slide method.Result In the whole city,10386 persons were surveyed and 498 were found to be infected with Clonorchis sinensis,with a total infection rate of 4.79%.The infection rates of male and female were 6.01% and 3.51%,respectively.The 50～59 age group showed the highest infection rate,and the infection rate of the key population was far higher than that of the general population.Gaoyao city,with an infection rate of 19.53%,was the most serious endemic area in the whole Zhaoqing city,and the next was Dinghu District,with an infection rate of 11.65%.Conclusion The mountainous counties in Zhaoqing city are low-epidemic areas of clonorchiasis,but the freshwater fish culture areas are the middle-epidemic or high-epidemic areas,including Gaoyao and Dinghu,which are the major areas of our control work against clonorchiasis.In order to lower the infection rate of Clonorchis sinensis and control the epidemic of clonorchiasis in Zhaoqing city,we suggest to enforcing the government in leading,and promoting all sections of the community to participate and taking the comprehensive control measure,mainly including behavior intervention and the survey and treatment to major areas and major crowds.
Peiming Zheng - One of the best experts on this subject based on the ideXlab platform.
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Gallbladder Stone Types in Patients with Cholelithiasis and Clonorchis sinensis Infection in the Endemic Area of Clonorchiasis
Zhongguo ji sheng chong xue yu ji sheng chong bing za zhi = Chinese journal of parasitology & parasitic diseases, 2015Co-Authors: Tie Qiao, Xiaobing Luo, Zhenliang Luo, Peiming Zheng, Liu-qing YangAbstract:OBJECTIVE To investigate the relationship between Clonorchis sinensis infection and the gallbladder stone type in patients with cholelithiasis in the endemic area of clonorchiasis. METHODS Gallbladder stones were collected from 598 patients with cholelithiasis through minimally invasive gallbladder-preserving cholelithotomy in the Sixth People's Hospital of Nansha District from May 2009 to October 2012. The stone samples were analyzed for composition by Fourier transform infrared spectroscopy to identify their types. The Clonorchis sinensis eggs were detected in the stones by microscopic examination, and the detection rates of eggs were calculated for different stone types. Then the clinical characteristics and biochemical indicators were compared among patients with different types of stones, as well as between Clonorchis sinensis egg-positive and -negative patients with the calcium-carbonate type of stones. Some calcium-carbonate stones positive for Clonorchis sinensis eggs were randomly selected for further scanning electron microscopy (SEM) examination. RESULTS Of the stones from 598 patients, 234 (39.1%) were cholesterol type, 133 (22.2%) bile pigment type, 112(18.7%) calcium-carbonate type, 86 (14.4%) mixed types and 33(5.5%) were others. The detection rate of Clonorchis sinensis eggs in these types was 6% (15/234), 44% (59/133), 60% (67/112), 36% (31/ 86) and 30% (10/33), respectively, being highest in calcium-carbonate stones while lowest in cholesterol stones. The Co2-combining power of the plasma was higher in patients with calcium-carbonate and mixed stones than in those with cholesterol stones (P < 0.05), and the CO2-combining power of the bile and biliary pH were both higher in patients with calcium-carbonate types than in those with other types (P < 0.05). In addition, in patients with calcium-carbonate stones, the CO2-combining powers of the plasma and the bile, as well as biliary pH were all higher in the egg-positives than in the egg-negatives. Further, both light microscopy and SEM revealed adherence of the Clonorchis sinensis eggs to calcium-carbonate crystals. CONCLUSION The infection rate of Clonorchis sinensis is higher in patients with calcium-carbonate gallbladder stones than in those with other types of stones.
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cholecystolithiasis is associated with Clonorchis sinensis infection
PLOS ONE, 2012Co-Authors: Tie Qiao, Xiaobing Luo, Zhenliang Luo, Peiming ZhengAbstract:Background The objective of this study was to analyze gallbladder stones for direct evidence of a relationship between Clonorchis sinensis infection and gallbladder stones formation. Methodology We investigated one hundred eighty-three gallbladder stones for the presence of Clonorchis sinensis eggs using microscopy, and analyzed their composition using Fourier transform infrared spectroscopy. We confirmed the presence of Clonorchis sinensis eggs in the gallbladder stones using real-time fluorescent PCR and scanning electron microscopy. Principal Findings Clonorchis sinensis eggs were detected in 122 of 183 gallbladder stones based on morphologic characteristics and results from real-time fluorescent PCR. The proportion of pigment stones, cholesterol stones and mixed gallstones in the egg-positive stones was 79.5% (97/122), 3.3% (4/122) and 17.2% (21/122), respectively, while 29.5% (18/61), 31.1% (19/61) and 39.3% (24/61) in the egg-negative stones. The proportion of pigment stone in the Clonorchis sinensis egg-positive stones was higher than in egg-negative stones (P<0.0001). In the 30 egg-positive stones examined by scanning electron microscopy, dozens or even hundreds of Clonorchis sinensis eggs were visible (×400) showing a distinct morphology. Many eggs were wrapped with surrounding particles, and in some, muskmelon wrinkles was seen on the surface of the eggs. Also visible were pieces of texture shed from some of the eggs. Some eggs were depressed or without operculum while most eggs were adhered to or wrapped with amorphous particles or mucoid matter (×3000). Conclusion Clonorchis sinensis eggs were detected in the gallbladder stones which suggests an association between Clonorchis sinensis infection and gallbladder stones formation, especially pigment stones.
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Cholecystolithiasis Is Associated with Clonorchis sinensis Infection
PloS one, 2012Co-Authors: Tie Qiao, Xiaobing Luo, Zhenliang Luo, Peiming ZhengAbstract:Background The objective of this study was to analyze gallbladder stones for direct evidence of a relationship between Clonorchis sinensis infection and gallbladder stones formation. Methodology We investigated one hundred eighty-three gallbladder stones for the presence of Clonorchis sinensis eggs using microscopy, and analyzed their composition using Fourier transform infrared spectroscopy. We confirmed the presence of Clonorchis sinensis eggs in the gallbladder stones using real-time fluorescent PCR and scanning electron microscopy. Principal Findings Clonorchis sinensis eggs were detected in 122 of 183 gallbladder stones based on morphologic characteristics and results from real-time fluorescent PCR. The proportion of pigment stones, cholesterol stones and mixed gallstones in the egg-positive stones was 79.5% (97/122), 3.3% (4/122) and 17.2% (21/122), respectively, while 29.5% (18/61), 31.1% (19/61) and 39.3% (24/61) in the egg-negative stones. The proportion of pigment stone in the Clonorchis sinensis egg-positive stones was higher than in egg-negative stones (P
Zhang Yong-l - One of the best experts on this subject based on the ideXlab platform.
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Cloning and Sequence Analysis of RNA Polymerase II Elongation Factor (RPEF) Gene of Clonorchis sinensis
Journal of Tropical Medicine, 2004Co-Authors: Zhang Yong-lAbstract:Objective To construct a recombinant plasmid and analyse encoding sequence of RPEF gene of Clonorchis sinensis. Methods A pair of primers was designed according to the known sequence of RPEF gene. The RPEF gene fragment was amplified by PCR. After purification and digestion with BamH Ⅰ and Sal Ⅰ, the RPEF gene was ligated to a prokaryotic expression vector, pET30a(+). Recombinant pET30a(+)-RPEF was constructed and transformed into E. coli BL21. Positive recombinants were detected by PCR, digestion with restriction enzyme and sequencing. Results The recombinant plasmid pET30a(+)-RPEF was constructed successfully. The result of sequence analysis showed that the RPEF protein of Clonorchis sinensis showed 81% of homology in the overall amino acid sequences with the mouse RNA polymerase Ⅱ elongation factor. Conclusion The construction of pET30a(+) -RPEF is important to further research on Clonorchis sinensis RPEF gene as a candidate target of anti- Clonorchis sinensis drug.
Tie Qiao - One of the best experts on this subject based on the ideXlab platform.
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Gallbladder Stone Types in Patients with Cholelithiasis and Clonorchis sinensis Infection in the Endemic Area of Clonorchiasis
Zhongguo ji sheng chong xue yu ji sheng chong bing za zhi = Chinese journal of parasitology & parasitic diseases, 2015Co-Authors: Tie Qiao, Xiaobing Luo, Zhenliang Luo, Peiming Zheng, Liu-qing YangAbstract:OBJECTIVE To investigate the relationship between Clonorchis sinensis infection and the gallbladder stone type in patients with cholelithiasis in the endemic area of clonorchiasis. METHODS Gallbladder stones were collected from 598 patients with cholelithiasis through minimally invasive gallbladder-preserving cholelithotomy in the Sixth People's Hospital of Nansha District from May 2009 to October 2012. The stone samples were analyzed for composition by Fourier transform infrared spectroscopy to identify their types. The Clonorchis sinensis eggs were detected in the stones by microscopic examination, and the detection rates of eggs were calculated for different stone types. Then the clinical characteristics and biochemical indicators were compared among patients with different types of stones, as well as between Clonorchis sinensis egg-positive and -negative patients with the calcium-carbonate type of stones. Some calcium-carbonate stones positive for Clonorchis sinensis eggs were randomly selected for further scanning electron microscopy (SEM) examination. RESULTS Of the stones from 598 patients, 234 (39.1%) were cholesterol type, 133 (22.2%) bile pigment type, 112(18.7%) calcium-carbonate type, 86 (14.4%) mixed types and 33(5.5%) were others. The detection rate of Clonorchis sinensis eggs in these types was 6% (15/234), 44% (59/133), 60% (67/112), 36% (31/ 86) and 30% (10/33), respectively, being highest in calcium-carbonate stones while lowest in cholesterol stones. The Co2-combining power of the plasma was higher in patients with calcium-carbonate and mixed stones than in those with cholesterol stones (P < 0.05), and the CO2-combining power of the bile and biliary pH were both higher in patients with calcium-carbonate types than in those with other types (P < 0.05). In addition, in patients with calcium-carbonate stones, the CO2-combining powers of the plasma and the bile, as well as biliary pH were all higher in the egg-positives than in the egg-negatives. Further, both light microscopy and SEM revealed adherence of the Clonorchis sinensis eggs to calcium-carbonate crystals. CONCLUSION The infection rate of Clonorchis sinensis is higher in patients with calcium-carbonate gallbladder stones than in those with other types of stones.
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cholecystolithiasis is associated with Clonorchis sinensis infection
PLOS ONE, 2012Co-Authors: Tie Qiao, Xiaobing Luo, Zhenliang Luo, Peiming ZhengAbstract:Background The objective of this study was to analyze gallbladder stones for direct evidence of a relationship between Clonorchis sinensis infection and gallbladder stones formation. Methodology We investigated one hundred eighty-three gallbladder stones for the presence of Clonorchis sinensis eggs using microscopy, and analyzed their composition using Fourier transform infrared spectroscopy. We confirmed the presence of Clonorchis sinensis eggs in the gallbladder stones using real-time fluorescent PCR and scanning electron microscopy. Principal Findings Clonorchis sinensis eggs were detected in 122 of 183 gallbladder stones based on morphologic characteristics and results from real-time fluorescent PCR. The proportion of pigment stones, cholesterol stones and mixed gallstones in the egg-positive stones was 79.5% (97/122), 3.3% (4/122) and 17.2% (21/122), respectively, while 29.5% (18/61), 31.1% (19/61) and 39.3% (24/61) in the egg-negative stones. The proportion of pigment stone in the Clonorchis sinensis egg-positive stones was higher than in egg-negative stones (P<0.0001). In the 30 egg-positive stones examined by scanning electron microscopy, dozens or even hundreds of Clonorchis sinensis eggs were visible (×400) showing a distinct morphology. Many eggs were wrapped with surrounding particles, and in some, muskmelon wrinkles was seen on the surface of the eggs. Also visible were pieces of texture shed from some of the eggs. Some eggs were depressed or without operculum while most eggs were adhered to or wrapped with amorphous particles or mucoid matter (×3000). Conclusion Clonorchis sinensis eggs were detected in the gallbladder stones which suggests an association between Clonorchis sinensis infection and gallbladder stones formation, especially pigment stones.
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Cholecystolithiasis Is Associated with Clonorchis sinensis Infection
PloS one, 2012Co-Authors: Tie Qiao, Xiaobing Luo, Zhenliang Luo, Peiming ZhengAbstract:Background The objective of this study was to analyze gallbladder stones for direct evidence of a relationship between Clonorchis sinensis infection and gallbladder stones formation. Methodology We investigated one hundred eighty-three gallbladder stones for the presence of Clonorchis sinensis eggs using microscopy, and analyzed their composition using Fourier transform infrared spectroscopy. We confirmed the presence of Clonorchis sinensis eggs in the gallbladder stones using real-time fluorescent PCR and scanning electron microscopy. Principal Findings Clonorchis sinensis eggs were detected in 122 of 183 gallbladder stones based on morphologic characteristics and results from real-time fluorescent PCR. The proportion of pigment stones, cholesterol stones and mixed gallstones in the egg-positive stones was 79.5% (97/122), 3.3% (4/122) and 17.2% (21/122), respectively, while 29.5% (18/61), 31.1% (19/61) and 39.3% (24/61) in the egg-negative stones. The proportion of pigment stone in the Clonorchis sinensis egg-positive stones was higher than in egg-negative stones (P
Min-ho Choi - One of the best experts on this subject based on the ideXlab platform.
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Results of the loop-mediated isothermal amplification (LAMP) assay for the detection of Clonorchis sinensis DNA in 120 stool samples compared with the results diagnosed by Kato-Katz (KK) and real-time PCR.
2017Co-Authors: S. Mazidur M. Rahman, Sung-tae Hong, Hyun Beom Song, Yan Jin, Min Kyung Lim, Min-ho ChoiAbstract:Results of the loop-mediated isothermal amplification (LAMP) assay for the detection of Clonorchis sinensis DNA in 120 stool samples compared with the results diagnosed by Kato-Katz (KK) and real-time PCR.
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Amplification of Clonorchis sinensis DNA in 120 stool samples by loop-mediated isothermal amplification (LAMP) according to the infection intensity presented as eggs per gram of feces (EPG).
2017Co-Authors: S. Mazidur M. Rahman, Sung-tae Hong, Hyun Beom Song, Yan Jin, Min Kyung Lim, Min-ho ChoiAbstract:Amplification of Clonorchis sinensis DNA in 120 stool samples by loop-mediated isothermal amplification (LAMP) according to the infection intensity presented as eggs per gram of feces (EPG).
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Specific and common antigens of Clonorchis sinensis and Opisthorchis viverrini(Opisthorchidae, Trematoda).
The Korean journal of parasitology, 2003Co-Authors: Min-ho Choi, Jong-yil Chai, Jin-sook Ryu, Mejeong Lee, Byung-suk Chung, Paiboon Sithithaworn, Smarn Tesana, Sung-tae HongAbstract:The antigenic characterizations and serological reactions of human liver flukes, Clonorchis sinensis and Opisthorchis viverrini, were analyzed by immunoblot. The antigenic profiles of the crude extract of Clonorchis contained major proteins of 8, 26-28, 34-37, 43, and 70 kDa, and those of Opisthorchis 34-37, 43, 70, and 100 kDa. Of these, the 8, 26-28 and 34-37 kDa bands of Clonorchis and the 100 kDa of Opisthorchis were major components of each excretory-secretory antigen. The 8 and 26-28 kDa bands were specific to Clonorchis but the 100 kDa of Opisthorchis cross-reacted with the sera of clonorchiasis, and the 34-37, 70 and 100 kDa bands cross-reacted with sera of other helminthiases. The frequency and intensity of the immunoblot reactions were positively correlated with the intensity of the liver fluke infection.