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Masayoshi Hisama - One of the best experts on this subject based on the ideXlab platform.
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antimutagenic activity of phenylpropanoids from Clove syzygium aromaticum
Journal of Agricultural and Food Chemistry, 2003Co-Authors: Mitsuo Miyazawa, Masayoshi HisamaAbstract:Phenylpropanoids that possess antimutagenic activity were isolated from the buds of Clove (Syzygium aromaticum). The isolated compounds suppressed the expression of the umu gene following the induction of SOS response in the Salmonella typhimurium TA1535/pSK1002 that have been treated with various mutagens. The suppressive compounds were mainly localized in the ethyl acetate extract fraction of the processed Clove. This ethyl acetate fraction was further fractionated by silica gel column chromatography, which resulted in the purification and subsequent identification of the suppressive compounds. Electron impact mass spectrometry, IR, and 1H and 13C NMR spectroscopy were then used to delineate the structures of the compounds that confer the observed antimutagenic activity. The secondary suppressive compounds were identified as dehydrodieugenol (1) and trans-coniferyl aldehyde (2). When using 2-(2-furyl)-3-(5-nitro-2-furyl)acrylamide (furylfuramide) as the mutagen, compound 1 suppressed 58% of the umu gene...
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antimutagenic activity of phenylpropanoids from Clove syzygium aromaticum
Journal of Agricultural and Food Chemistry, 2003Co-Authors: Mitsuo Miyazawa, Masayoshi HisamaAbstract:Phenylpropanoids that possess antimutagenic activity were isolated from the buds of Clove (Syzygium aromaticum). The isolated compounds suppressed the expression of the umu gene following the induction of SOS response in the Salmonella typhimurium TA1535/pSK1002 that have been treated with various mutagens. The suppressive compounds were mainly localized in the ethyl acetate extract fraction of the processed Clove. This ethyl acetate fraction was further fractionated by silica gel column chromatography, which resulted in the purification and subsequent identification of the suppressive compounds. Electron impact mass spectrometry, IR, and (1)H and (13)C NMR spectroscopy were then used to delineate the structures of the compounds that confer the observed antimutagenic activity. The secondary suppressive compounds were identified as dehydrodieugenol (1) and trans-coniferyl aldehyde (2). When using 2-(2-furyl)-3-(5-nitro-2-furyl)acrylamide (furylfuramide) as the mutagen, compound 1 suppressed 58% of the umu gene expression as compared to the controls at a concentration of 0.60 micromol/mL, with an ID(50) (50% inhibitory dose) value of 0.48 micromol/mL, and compound 2 suppressed 63% of the umu gene expression as compared to the controls at a concentration of 1.20 micromol/mL, with an ID(50) value of 0.76 micromol/mL. Additionally, compounds 1 and 2 were tested for their ability to suppress the mutagenic activity of other well-known mutagens such as 4-nitroquinolin 1-oxide (4NQO) and N-methyl-N'-nitro-N-nitrosoguanidine (MNNG), which do not require liver metabolizing enzymes, and aflatoxin B(1) (AfB(1)) and 3-amino-1,4-dimethyl-5H-pyrido[4,3-b]indole (Trp-P-1), which require liver metabolizing enzymes and activated Trp-P-1 and UV irradiation. Compounds 1 and 2 showed dramatic reductions in their mutagenic potential of all of the aforementioned chemicals or treatment. For the search of the structure-activity relationship, the derivatives of 1 and 2 (1a and 2a-c) were also assayed with all mutagens. Finally, the antimutagenic activities of compounds 1, 1a, 2, and 2a-c against furylfuramide, Trp-P-1, and activated Trp-P-1 were assayed by the Ames test using the S. typhimurium TA100 strain.
Mitsuo Miyazawa - One of the best experts on this subject based on the ideXlab platform.
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antimutagenic activity of phenylpropanoids from Clove syzygium aromaticum
Journal of Agricultural and Food Chemistry, 2003Co-Authors: Mitsuo Miyazawa, Masayoshi HisamaAbstract:Phenylpropanoids that possess antimutagenic activity were isolated from the buds of Clove (Syzygium aromaticum). The isolated compounds suppressed the expression of the umu gene following the induction of SOS response in the Salmonella typhimurium TA1535/pSK1002 that have been treated with various mutagens. The suppressive compounds were mainly localized in the ethyl acetate extract fraction of the processed Clove. This ethyl acetate fraction was further fractionated by silica gel column chromatography, which resulted in the purification and subsequent identification of the suppressive compounds. Electron impact mass spectrometry, IR, and 1H and 13C NMR spectroscopy were then used to delineate the structures of the compounds that confer the observed antimutagenic activity. The secondary suppressive compounds were identified as dehydrodieugenol (1) and trans-coniferyl aldehyde (2). When using 2-(2-furyl)-3-(5-nitro-2-furyl)acrylamide (furylfuramide) as the mutagen, compound 1 suppressed 58% of the umu gene...
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antimutagenic activity of phenylpropanoids from Clove syzygium aromaticum
Journal of Agricultural and Food Chemistry, 2003Co-Authors: Mitsuo Miyazawa, Masayoshi HisamaAbstract:Phenylpropanoids that possess antimutagenic activity were isolated from the buds of Clove (Syzygium aromaticum). The isolated compounds suppressed the expression of the umu gene following the induction of SOS response in the Salmonella typhimurium TA1535/pSK1002 that have been treated with various mutagens. The suppressive compounds were mainly localized in the ethyl acetate extract fraction of the processed Clove. This ethyl acetate fraction was further fractionated by silica gel column chromatography, which resulted in the purification and subsequent identification of the suppressive compounds. Electron impact mass spectrometry, IR, and (1)H and (13)C NMR spectroscopy were then used to delineate the structures of the compounds that confer the observed antimutagenic activity. The secondary suppressive compounds were identified as dehydrodieugenol (1) and trans-coniferyl aldehyde (2). When using 2-(2-furyl)-3-(5-nitro-2-furyl)acrylamide (furylfuramide) as the mutagen, compound 1 suppressed 58% of the umu gene expression as compared to the controls at a concentration of 0.60 micromol/mL, with an ID(50) (50% inhibitory dose) value of 0.48 micromol/mL, and compound 2 suppressed 63% of the umu gene expression as compared to the controls at a concentration of 1.20 micromol/mL, with an ID(50) value of 0.76 micromol/mL. Additionally, compounds 1 and 2 were tested for their ability to suppress the mutagenic activity of other well-known mutagens such as 4-nitroquinolin 1-oxide (4NQO) and N-methyl-N'-nitro-N-nitrosoguanidine (MNNG), which do not require liver metabolizing enzymes, and aflatoxin B(1) (AfB(1)) and 3-amino-1,4-dimethyl-5H-pyrido[4,3-b]indole (Trp-P-1), which require liver metabolizing enzymes and activated Trp-P-1 and UV irradiation. Compounds 1 and 2 showed dramatic reductions in their mutagenic potential of all of the aforementioned chemicals or treatment. For the search of the structure-activity relationship, the derivatives of 1 and 2 (1a and 2a-c) were also assayed with all mutagens. Finally, the antimutagenic activities of compounds 1, 1a, 2, and 2a-c against furylfuramide, Trp-P-1, and activated Trp-P-1 were assayed by the Ames test using the S. typhimurium TA100 strain.
Mayumi Ikegamikawai - One of the best experts on this subject based on the ideXlab platform.
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analysis of volatile compounds of Clove syzygium aromaticum buds as influenced by growth phase and investigation of antioxidant activity of Clove extracts
Flavour and Fragrance Journal, 2016Co-Authors: Hiroko Kasai, Mika Shirao, Mayumi IkegamikawaiAbstract:The volatile compounds originating from fresh Clove (Syzygium aromaticum) buds cultivated in a medicinal plant garden located in southern Tokyo were investigated. The volatile compounds originating from fresh Clove buds were adsorbed onto polydimethylsiloxane-coated devices and then analysed using gas chromatography-mass spectrometry. Sampling was performed at three different development stages of Clove buds, i.e., phase I (green), phase II (pink), and phase III (red). In thermal desorption-gas chromatography-mass spectrometry procedure volatile compounds: eugenol, β-caryophyllene, α-humulene, α-farnesene, and eugenol acetate were the predominant constituents in most cases. Methyl salicylate, α-copaene, β-ocimene, 2-nonanone, ethyl hexanoate, and acetophenone were additionally found as the buds ripened. Volatile compounds obtained from Clove leaves were analysed using the same method, and β-caryophyllene, eugenol, α-humulene, 3-hexen-1-ol, 3-hexen-1-yl acetate, and hexyl acetate were identified. The antioxidant activity of both water and methanol extracts of Clove buds and leaves collected from the medicinal plant garden was confirmed using electron spin resonance spectrometry. Scavenging activities against superoxide anion radical and hydroxyl radical were evaluated. Copyright © 2015 John Wiley & Sons, Ltd.
Paramita Bhattacharjee - One of the best experts on this subject based on the ideXlab platform.
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Supercritical Carbon Dioxide Extraction of Eugenol from Clove Buds
Food and Bioprocess Technology, 2013Co-Authors: Dipan Chatterjee, Paramita BhattacharjeeAbstract:Isolation of eugenol from dried Clove buds ( Syzygium aromaticum Linn) of Indian origin was carried out using supercritical carbon dioxide (SC-CO_2) extraction. Optimization of its process parameters such as temperature, pressure and time of extraction has been conducted using response surface methodology. The optimized conditions that provided the optimum yield of eugenol were a sample size of 20 g of Clove powder of particle diameter 0.5 mm at a temperature of 60°C and pressure of 250 bar for 90 min extracting time at a flow rate of 2 l min^−1 of CO_2. Statistical analyses conducted on the extraction parameters concluded that extraction temperature have significant effect on the yield of eugenol, whereas extraction pressure and time do not. The results obtained are in accordance to the basic principle of supercritical fluid phase equilibrium behavior. Solubility of eugenol in SC-CO_2 under different temperature and pressure regimes have been determined experimentally and values were used to construct a correlated Chrastil equation in linear form to allow the prediction of solubility of eugenol in SC-CO_2 under different extraction conditions. Using dimensionless numbers, an empirical correlation was also deduced for characterization of the extraction process of eugenol in SC-CO_2 considering overall fixed bed behavior for Clove matrix and mass transfer coefficients in supercritical phases.
Hiroko Kasai - One of the best experts on this subject based on the ideXlab platform.
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analysis of volatile compounds of Clove syzygium aromaticum buds as influenced by growth phase and investigation of antioxidant activity of Clove extracts
Flavour and Fragrance Journal, 2016Co-Authors: Hiroko Kasai, Mika Shirao, Mayumi IkegamikawaiAbstract:The volatile compounds originating from fresh Clove (Syzygium aromaticum) buds cultivated in a medicinal plant garden located in southern Tokyo were investigated. The volatile compounds originating from fresh Clove buds were adsorbed onto polydimethylsiloxane-coated devices and then analysed using gas chromatography-mass spectrometry. Sampling was performed at three different development stages of Clove buds, i.e., phase I (green), phase II (pink), and phase III (red). In thermal desorption-gas chromatography-mass spectrometry procedure volatile compounds: eugenol, β-caryophyllene, α-humulene, α-farnesene, and eugenol acetate were the predominant constituents in most cases. Methyl salicylate, α-copaene, β-ocimene, 2-nonanone, ethyl hexanoate, and acetophenone were additionally found as the buds ripened. Volatile compounds obtained from Clove leaves were analysed using the same method, and β-caryophyllene, eugenol, α-humulene, 3-hexen-1-ol, 3-hexen-1-yl acetate, and hexyl acetate were identified. The antioxidant activity of both water and methanol extracts of Clove buds and leaves collected from the medicinal plant garden was confirmed using electron spin resonance spectrometry. Scavenging activities against superoxide anion radical and hydroxyl radical were evaluated. Copyright © 2015 John Wiley & Sons, Ltd.