The Experts below are selected from a list of 10038 Experts worldwide ranked by ideXlab platform

Behzad Kaviani - One of the best experts on this subject based on the ideXlab platform.

  • Effect of Cobalt Chloride on vase life and postharvest quality of cut tuberose(Polianthes tuberosa L.)
    European Journal of Experimental Biology, 2012
    Co-Authors: Mohsen Mohammadi, Davood Hashemabadi, Behzad Kaviani
    Abstract:

    In order to evaluate the effect of Cobalt Chloride on vase life and postharvest of cut tuberose, an experiment based on complete randomized design with 4 levels of Cobalt Chloride (0, 200, 300, 400 mg l-1) in 3 replications was conducted. Statistical analysis showed that the effect of Cobalt Chloride on measured traits was significant at 5 and 1% probability level. Mean comparison of the data showed that the 300 mg l-1 of Cobalt Chloride had the highest effect on longer vase life (10.66 days), water absorption (1.53 ml g-1 F.W.) and loss of fresh weight (19.99 g). Also, the 400 mg l-1 of Cobalt Chloride showed the maximum effect on petal’s carotenoids (0.40 g) and proteins (31.10%).

Fanfan Zhou - One of the best experts on this subject based on the ideXlab platform.

  • a derivative of betulinic acid protects human retinal pigment epithelial rpe cells from Cobalt Chloride induced acute hypoxic stress
    Experimental Eye Research, 2019
    Co-Authors: Zhengqi Cheng, Jian Zheng, Weimin Ding, Yang Wang, Ting Zhang, Fanfan Zhou
    Abstract:

    Abstract The Retinal Pigment Epithelium (RPE) is a monolayer of cells located above the choroid. It mediates human visual cycle and nourishes photoreceptors. Hypoxia-induced oxidative stress to RPE is a vital cause of retinal degeneration such as the Age-related Macular Degeneration. Most of these retinal diseases are irreversible with no efficient treatment, therefore protecting RPE cells from hypoxia stress is an important way to prevent or slow down the progression of retinal degeneration. Betulinic acid (BA) and betulin (BE) are pentacyclic triterpenoids with anti-oxidative property, but little is known about their effect on RPE cells. We investigated the protective effect of BA, BE and their derivatives against Cobalt Chloride-induced hypoxia stress in RPE cells. Human ARPE-19 cells were exposed to BA, BE and their eighteen derivatives (named as H3 H20) that we customized through replacing moieties at C3 and C28 positions. We found that Cobalt Chloride reduced cell viability, increased Reactive Oxygen Species (ROS) production as well as induced apoptosis and necrosis in ARPE-19 cells. Interestingly, the pretreatment of 3-O-acetyl-glycyl- 28-O-glycyl-betulinic acid effectively protected cells from acute hypoxia stress induced by Cobalt Chloride. Our immunoblotting results suggested that this derivative attenuated the Cobalt Chloride-induced activation of Akt, Erk and JNK pathways. All findings were further validated in human primary RPE cells. In summary, this BA derivate has protective effect against the acute hypoxic stress in human RPE cells and may be developed into a candidate agent effective in the prevention of prevalent retinal diseases.

Mohsen Mohammadi - One of the best experts on this subject based on the ideXlab platform.

  • Effect of Cobalt Chloride on vase life and postharvest quality of cut tuberose(Polianthes tuberosa L.)
    European Journal of Experimental Biology, 2012
    Co-Authors: Mohsen Mohammadi, Davood Hashemabadi, Behzad Kaviani
    Abstract:

    In order to evaluate the effect of Cobalt Chloride on vase life and postharvest of cut tuberose, an experiment based on complete randomized design with 4 levels of Cobalt Chloride (0, 200, 300, 400 mg l-1) in 3 replications was conducted. Statistical analysis showed that the effect of Cobalt Chloride on measured traits was significant at 5 and 1% probability level. Mean comparison of the data showed that the 300 mg l-1 of Cobalt Chloride had the highest effect on longer vase life (10.66 days), water absorption (1.53 ml g-1 F.W.) and loss of fresh weight (19.99 g). Also, the 400 mg l-1 of Cobalt Chloride showed the maximum effect on petal’s carotenoids (0.40 g) and proteins (31.10%).

Ting Zhang - One of the best experts on this subject based on the ideXlab platform.

  • a derivative of betulinic acid protects human retinal pigment epithelial rpe cells from Cobalt Chloride induced acute hypoxic stress
    Experimental Eye Research, 2019
    Co-Authors: Zhengqi Cheng, Jian Zheng, Weimin Ding, Yang Wang, Ting Zhang, Fanfan Zhou
    Abstract:

    Abstract The Retinal Pigment Epithelium (RPE) is a monolayer of cells located above the choroid. It mediates human visual cycle and nourishes photoreceptors. Hypoxia-induced oxidative stress to RPE is a vital cause of retinal degeneration such as the Age-related Macular Degeneration. Most of these retinal diseases are irreversible with no efficient treatment, therefore protecting RPE cells from hypoxia stress is an important way to prevent or slow down the progression of retinal degeneration. Betulinic acid (BA) and betulin (BE) are pentacyclic triterpenoids with anti-oxidative property, but little is known about their effect on RPE cells. We investigated the protective effect of BA, BE and their derivatives against Cobalt Chloride-induced hypoxia stress in RPE cells. Human ARPE-19 cells were exposed to BA, BE and their eighteen derivatives (named as H3 H20) that we customized through replacing moieties at C3 and C28 positions. We found that Cobalt Chloride reduced cell viability, increased Reactive Oxygen Species (ROS) production as well as induced apoptosis and necrosis in ARPE-19 cells. Interestingly, the pretreatment of 3-O-acetyl-glycyl- 28-O-glycyl-betulinic acid effectively protected cells from acute hypoxia stress induced by Cobalt Chloride. Our immunoblotting results suggested that this derivative attenuated the Cobalt Chloride-induced activation of Akt, Erk and JNK pathways. All findings were further validated in human primary RPE cells. In summary, this BA derivate has protective effect against the acute hypoxic stress in human RPE cells and may be developed into a candidate agent effective in the prevention of prevalent retinal diseases.

William R. Martin - One of the best experts on this subject based on the ideXlab platform.

  • Analgesic actions of local anesthetics and Cobalt Chloride in the rat brain stem
    Pharmacology biochemistry and behavior, 1992
    Co-Authors: S.r. Hamann, Joseph R. Holtman, William R. Martin
    Abstract:

    A low-intensity thermally evoked tail avoidance reflex (LITETAR) was used to study changes in nociceptive response produced by local anesthetics and Cobalt Chloride microinjected into the dorsal posterior mesencephalic tegmentum (DPMT) of conscious rats. Dose-related prolongation of the LITETAR (e.g., analgesia) was observed when lidocaine, cocaine, and bupivacaine were administered into the DPMT. Analgesic actions were also demonstrated when Cobalt Chloride was microinjected into the DPMT. The analgesic actions of these different neuronal suppressants provide support for the hypothesis that there exists tonic activity of hyperalgesic processes in the rat brain stem.