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Vendula Slechtova - One of the best experts on this subject based on the ideXlab platform.
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molecular phylogeny of the freshwater fish family Cobitidae cypriniformes teleostei delimitation of genera mitochondrial introgression and evolution of sexual dimorphism
Molecular Phylogenetics and Evolution, 2008Co-Authors: Vendula Slechtova, Jorg Bohlen, Anabel PerdicesAbstract:Abstract The family Cobitidae represents a characteristic element of the Eurasian ichthyofauna. Despite diverse features of sexual dimorphism, comparably few morphological characters have been utilized for taxonomic studies resulting in many unresolved puzzles. Here we present the phylogenetic relationships of Cobitidae as inferred from the mitochondrial cytochrome b gene and the nuclear gene RAG-1. Analyses of both markers show a group of eight nominal genera, which all occur in Europe and eastern, northern and western Asia, forming a monophyletic lineage (northern clade) while all other clades inhabit South and Southeast Asia (southern lineages). While all eight southern lineages correspond to genera as defined by morphological studies, only four lineages were reliably recovered within the northern clade, and of these only one (Sabanejewia) corresponds to a formerly considered genus. The genera Cobitis, Iksookimia and Niwaella were polyphyletic. A comparison of the two markers shows several incongruities within the northern clade and mitochondrial introgression at least in the genus Misgurnus. Mapping the characters of sexual dimorphism on our cladogram, we identified five character states that are diagnostic for certain lineages. Estimations of the divergence times dated the separation of the northern clade from the southern lineages to the middle Eocene (46 MYA) and the origin of “Cobitis” misgurnoides, the basal taxon of the northern clade, during early Oligocene (30–35 MYA). The geographic distribution of the major clades supports recently developed hypotheses about the river history of East Asia and further suggests that a range expansion of the northern clade in late Miocene (15 MYA) led to the colonisation of Europe by three already distinct genera.
Yong-jin Won - One of the best experts on this subject based on the ideXlab platform.
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Genetic patterns reveal northward range expansion and cryptic diversity in Nalbant's spined loach, Cobitis nalbanti sensu lato (Teleostei: Cypriniformes: Cobitidae)
Systematics and Biodiversity, 2020Co-Authors: Yeon Seon Jeon, Ekaterina D. Vasil’eva, Ra-yeon Myung, Yong-jin WonAbstract:The morphologically similar populations of the Korean freshwater spined loach recently described as a new species Cobitis nalbanti (Actinopterygii: Cypriniformes: Cobitidae) inhabit the rivers and ...
Katsutoshi Arai - One of the best experts on this subject based on the ideXlab platform.
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Microsatellite loci in the tetraploid spined loach, Cobitis biwae, and cross-species amplification in four related species
Genetics and molecular research : GMR, 2016Co-Authors: Olga Jablonska, Takafumi Fujimoto, Alan Marín, K. Kowalewska, Katsutoshi AraiAbstract:Fifteen microsatellite loci were identified in the tetraploid spined loach, Cobitis biwae (Teleostei: Cobitidae). Among these, 14 were polymorphic (5-31 alleles) and showed moderate to high cross-species amplification transferability in four related species, Cobitis matsubarai, Cobitis taenia, Misgurnus anguillicaudatus, and Misgurnus fossilis. The loci, described herein, will be useful for population genetics, phylogeny, parentage analysis, and detection of hybridization among Cobitis species.
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androgenetic doubled haploids induced without irradiation of eggs in loach misgurnus anguillicaudatus
Aquaculture, 2014Co-Authors: Jilun Hou, Takafumi Fujimoto, Etsuro Yamaha, Taiju Saito, Katsutoshi AraiAbstract:Abstract Androgenetic doubled haploids (DHs) were induced in the loach Misgurnus anguillicaudatus (Cobitidae) without irradiation of the eggs. The eggs of wild-type females were activated with the intact sperm of an orange-phenotype male, and treated (within 10 s of activation) at 3 ± 0.5 °C for 30 min, to eliminate the female nucleus. The eggs were then incubated in a water bath at 20 ± 0.5 °C for 35 min. Finally, diploidy was restored (65 min after activation) by heat-shock treatment at 42 ± 0.5 °C for 2 min. Under these conditions, the yield rate (mean ± SD) of putative DHs relative to the total number of eggs used was 10.43 ± 1.69%, which was significantly higher (P
Anabel Perdices - One of the best experts on this subject based on the ideXlab platform.
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molecular phylogeny of the freshwater fish family Cobitidae cypriniformes teleostei delimitation of genera mitochondrial introgression and evolution of sexual dimorphism
Molecular Phylogenetics and Evolution, 2008Co-Authors: Vendula Slechtova, Jorg Bohlen, Anabel PerdicesAbstract:Abstract The family Cobitidae represents a characteristic element of the Eurasian ichthyofauna. Despite diverse features of sexual dimorphism, comparably few morphological characters have been utilized for taxonomic studies resulting in many unresolved puzzles. Here we present the phylogenetic relationships of Cobitidae as inferred from the mitochondrial cytochrome b gene and the nuclear gene RAG-1. Analyses of both markers show a group of eight nominal genera, which all occur in Europe and eastern, northern and western Asia, forming a monophyletic lineage (northern clade) while all other clades inhabit South and Southeast Asia (southern lineages). While all eight southern lineages correspond to genera as defined by morphological studies, only four lineages were reliably recovered within the northern clade, and of these only one (Sabanejewia) corresponds to a formerly considered genus. The genera Cobitis, Iksookimia and Niwaella were polyphyletic. A comparison of the two markers shows several incongruities within the northern clade and mitochondrial introgression at least in the genus Misgurnus. Mapping the characters of sexual dimorphism on our cladogram, we identified five character states that are diagnostic for certain lineages. Estimations of the divergence times dated the separation of the northern clade from the southern lineages to the middle Eocene (46 MYA) and the origin of “Cobitis” misgurnoides, the basal taxon of the northern clade, during early Oligocene (30–35 MYA). The geographic distribution of the major clades supports recently developed hypotheses about the river history of East Asia and further suggests that a range expansion of the northern clade in late Miocene (15 MYA) led to the colonisation of Europe by three already distinct genera.
J. K. J. Van Houdt - One of the best experts on this subject based on the ideXlab platform.
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Development of nine polymorphic microsatellite loci in the spined loach, Cobitis taenia, and cross-species amplification in the related species C. elongatoides, C. taurica and C. tanaitica.
Molecular ecology resources, 2008Co-Authors: K. De Gelas, Karel Janko, F. A. M. Volckaert, D. De Charleroy, J. K. J. Van HoudtAbstract:Nine polymorphic microsatellite loci were developed for the spined loach, Cobitis taenia (Teleostei: Cobitidae). The loci were validated using 50 individuals from a population in Belgium. Moderate to high levels of polymorphism were detected (two to 11 alleles). In addition, most markers amplified successfully in three closely related taxa that are known to hybridize with C. taenia: C. elongatoides, C. taurica and C. tanaitica. Some of the loci are most likely diagnostic among species. These markers will be valuable for the study of the historical and contemporary interactions within C. taenia and the Cobitis species complex.