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Josep Guarro - One of the best experts on this subject based on the ideXlab platform.
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DNA sequencing to clarify the taxonomical conundrum of the clinical Coelomycetes.
Mycoses, 2018Co-Authors: Nicomedes Valenzuela-lopez, José F. Cano-lira, Alberto M. Stchigel, Josep GuarroAbstract:The taxonomy of the fungi that produce human infections and that develop asexual fruiting bodies in culture has become very complex. Recent molecular studies have produced dramatic changes in their classification. Currently, the Coelomycetes traditionally included in Sphaeropsidales and Melanconiales are in fact distributed across at least three different classes of the Phylum Ascomycota. Approximately 1000 genera and 7000 species have been grouped in the classes Dothideomycetes, Leotiomycetes and Sordariomycetes and their proper identification can only be made by analysing their DNA sequences and comparing them with those corresponding to type strains available in the adequate databases. To facilitate this task for scientists and clinicians involved in the study of these complex, and every day more numerous taxa, we have updated the knowledge about the taxonomy of the commonest Coelomycetes of clinical interest with the aim of improving their identification and antifungal treatment.
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Coelomycetous Dothideomycetes with emphasis on the families Cucurbitariaceae and Didymellaceae.
Studies in mycology, 2017Co-Authors: Nicomedes Valenzuela-lopez, Josep Guarro, Pedro W. Crous, Deanna A. Sutton, J. F. Cano-lira, Nathan P. Wiederhold, Alberto M. StchigelAbstract:The taxonomy of the Coelomycetes has undergone dramatic changes in recent years, but remains controversial due to the high number of taxa involved, their poor morphological differentiation, the rare occurrence of the sexual morphs, and rapid loss of fertility in vitro. In the present study, we revisited the families Cucurbitariaceae and Didymellaceae (Pleosporales, Dothideomycetes), which include numerous plant pathogens, endophytic species associated with a wide host range, and saprobes. The taxonomy of two of the most relevant genera, i.e. Phoma and Pyrenochaeta, remains ambiguous after several phylogenetic studies, and needs further revision. We have studied a total of 143 strains of Coelomycetes from clinical or environmental origin, by combining the LSU, ITS, tub2 and rpb2 sequences for a multi-locus analysis and a detailed morphological comparison. The resulting phylogenetic tree revealed that some fungi previously considered as members of Cucurbitariaceae represented five different families, and four of them, Neopyrenochaetaceae, Parapyrenochaetaceae, Pseudopyrenochaetaceae and Pyrenochaetopsidaceae, are proposed here as new. Furthermore, 13 new genera, 28 new species, and 20 new combinations are proposed within the Pleosporineae. Moreover, four new typifications are introduced to stabilise the taxonomy of these fungi.
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New Pyrenochaeta Species Causing Keratitis
Journal of clinical microbiology, 2009Co-Authors: Consuelo Ferrer, Gerard J.m. Verkley, Juan J Pérez-santonja, Alejandra E. Rodriguez, M. F. Colom, Josepa Gené, J. L. Alio, Josep GuarroAbstract:We report a new fungus as an agent of fungal keratitis in a diabetic woman. The fungal etiology was established by classic microbiology and PCR following 3 months of antibacterial therapy. The morphological features of the isolate and sequence analysis of the internal transcribed spacer region indicate a new species of Pyrenochaeta (Coelomycetes).
Olivier Lortholary - One of the best experts on this subject based on the ideXlab platform.
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Ten-Year Experience of Cutaneous and/or Subcutaneous Infections Due to Coelomycetes in France
Open Forum Infectious Diseases, 2016Co-Authors: Sarah Guégan, Dea Garcia-hermoso, Karine Sitbon, Sarah Ahmed, Philippe Moguelet, Françoise Dromer, Olivier Lortholary, French Mycosis Study GroupAbstract:Background. Coelomycetes are rarely but increasingly reported in association with human infections involving mostly skin and subcutaneous tissues, both in immunocompetent and immunocompromised patients. Coelomycetes constitute a heterogeneous group of filamentous fungi with distinct morphological characteristics in culture, namely an ability to produce asexual spores within fruit bodies. Methods. We included all cases of proven primary cutaneous and/or subcutaneous infections due to Coelomycetes received for identification at the French National Reference Center for Invasive Mycoses and Antifungals between 2005 and 2014. Eumycetoma, chromoblastomycosis, and disseminated infections were excluded. Results. Eighteen cases were analyzed. The median age was 60.5 years. In all cases, patients originated from tropical or subtropical areas. An underlying immunodepression was present in 89% of cases. Cutaneous and/or subcutaneous lesions, mainly nodules, abscesses, or infiltrated plaques, were observed in distal body areas. Isolates of different genera of Coelomycetes were identified: Medicopsis (6), Paraconiothyrium (3), Gloniopsis (3), Diaporthe (3), Peyronellaea (2), Lasiodiplodia (1). Lesion treatment consisted of complete (10) or partial (2) surgical excision and/or the use of systemic antifungal therapy, namely voriconazole (5) and posaconazole (4). Literature review yielded 48 additional cases of cutaneous and/or subcutaneous infections due to Coelomycetes. Conclusions. Infectious diseases physicians should suspect Coelomycetes when observing cutaneous and/or subcutaneous infections in immunocompromised hosts from tropical areas; a sequence-based approach is crucial for strains identification but must be supported by consistent phenotypic features; surgical treatment should be favored for solitary, well limited lesions; new triazoles may be used in case of extensive lesions, especially in immunocompromised patients.
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ten year experience of cutaneous and or subcutaneous infections due to Coelomycetes in france
Open Forum Infectious Diseases, 2016Co-Authors: Sarah Guégan, Karine Sitbon, Philippe Moguelet, Françoise Dromer, Dea Garciahermoso, Sarah A Ahmed, Olivier LortholaryAbstract:Background. Coelomycetes are rarely but increasingly reported in association with human infections involving mostly skin and subcutaneous tissues, both in immunocompetent and immunocompromised patients. Coelomycetes constitute a heterogeneous group of filamentous fungi with distinct morphological characteristics in culture, namely an ability to produce asexual spores within fruit bodies. Methods. We included all cases of proven primary cutaneous and/or subcutaneous infections due to Coelomycetes received for identification at the French National Reference Center for Invasive Mycoses and Antifungals between 2005 and 2014. Eumycetoma, chromoblastomycosis, and disseminated infections were excluded. Results. Eighteen cases were analyzed. The median age was 60.5 years. In all cases, patients originated from tropical or subtropical areas. An underlying immunodepression was present in 89% of cases. Cutaneous and/or subcutaneous lesions, mainly nodules, abscesses, or infiltrated plaques, were observed in distal body areas. Isolates of different genera of Coelomycetes were identified: Medicopsis (6), Paraconiothyrium (3), Gloniopsis (3), Diaporthe (3), Peyronellaea (2), Lasiodiplodia (1). Lesion treatment consisted of complete (10) or partial (2) surgical excision and/or the use of systemic antifungal therapy, namely voriconazole (5) and posaconazole (4). Literature review yielded 48 additional cases of cutaneous and/or subcutaneous infections due to Coelomycetes. Conclusions. Infectious diseases physicians should suspect Coelomycetes when observing cutaneous and/or subcutaneous infections in immunocompromised hosts from tropical areas; a sequence-based approach is crucial for strains identification but must be supported by consistent phenotypic features; surgical treatment should be favored for solitary, well limited lesions; new triazoles may be used in case of extensive lesions, especially in immunocompromised patients.
Sarah Guégan - One of the best experts on this subject based on the ideXlab platform.
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Ten-Year Experience of Cutaneous and/or Subcutaneous Infections Due to Coelomycetes in France
Open Forum Infectious Diseases, 2016Co-Authors: Sarah Guégan, Dea Garcia-hermoso, Karine Sitbon, Sarah Ahmed, Philippe Moguelet, Françoise Dromer, Olivier Lortholary, French Mycosis Study GroupAbstract:Background. Coelomycetes are rarely but increasingly reported in association with human infections involving mostly skin and subcutaneous tissues, both in immunocompetent and immunocompromised patients. Coelomycetes constitute a heterogeneous group of filamentous fungi with distinct morphological characteristics in culture, namely an ability to produce asexual spores within fruit bodies. Methods. We included all cases of proven primary cutaneous and/or subcutaneous infections due to Coelomycetes received for identification at the French National Reference Center for Invasive Mycoses and Antifungals between 2005 and 2014. Eumycetoma, chromoblastomycosis, and disseminated infections were excluded. Results. Eighteen cases were analyzed. The median age was 60.5 years. In all cases, patients originated from tropical or subtropical areas. An underlying immunodepression was present in 89% of cases. Cutaneous and/or subcutaneous lesions, mainly nodules, abscesses, or infiltrated plaques, were observed in distal body areas. Isolates of different genera of Coelomycetes were identified: Medicopsis (6), Paraconiothyrium (3), Gloniopsis (3), Diaporthe (3), Peyronellaea (2), Lasiodiplodia (1). Lesion treatment consisted of complete (10) or partial (2) surgical excision and/or the use of systemic antifungal therapy, namely voriconazole (5) and posaconazole (4). Literature review yielded 48 additional cases of cutaneous and/or subcutaneous infections due to Coelomycetes. Conclusions. Infectious diseases physicians should suspect Coelomycetes when observing cutaneous and/or subcutaneous infections in immunocompromised hosts from tropical areas; a sequence-based approach is crucial for strains identification but must be supported by consistent phenotypic features; surgical treatment should be favored for solitary, well limited lesions; new triazoles may be used in case of extensive lesions, especially in immunocompromised patients.
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ten year experience of cutaneous and or subcutaneous infections due to Coelomycetes in france
Open Forum Infectious Diseases, 2016Co-Authors: Sarah Guégan, Karine Sitbon, Philippe Moguelet, Françoise Dromer, Dea Garciahermoso, Sarah A Ahmed, Olivier LortholaryAbstract:Background. Coelomycetes are rarely but increasingly reported in association with human infections involving mostly skin and subcutaneous tissues, both in immunocompetent and immunocompromised patients. Coelomycetes constitute a heterogeneous group of filamentous fungi with distinct morphological characteristics in culture, namely an ability to produce asexual spores within fruit bodies. Methods. We included all cases of proven primary cutaneous and/or subcutaneous infections due to Coelomycetes received for identification at the French National Reference Center for Invasive Mycoses and Antifungals between 2005 and 2014. Eumycetoma, chromoblastomycosis, and disseminated infections were excluded. Results. Eighteen cases were analyzed. The median age was 60.5 years. In all cases, patients originated from tropical or subtropical areas. An underlying immunodepression was present in 89% of cases. Cutaneous and/or subcutaneous lesions, mainly nodules, abscesses, or infiltrated plaques, were observed in distal body areas. Isolates of different genera of Coelomycetes were identified: Medicopsis (6), Paraconiothyrium (3), Gloniopsis (3), Diaporthe (3), Peyronellaea (2), Lasiodiplodia (1). Lesion treatment consisted of complete (10) or partial (2) surgical excision and/or the use of systemic antifungal therapy, namely voriconazole (5) and posaconazole (4). Literature review yielded 48 additional cases of cutaneous and/or subcutaneous infections due to Coelomycetes. Conclusions. Infectious diseases physicians should suspect Coelomycetes when observing cutaneous and/or subcutaneous infections in immunocompromised hosts from tropical areas; a sequence-based approach is crucial for strains identification but must be supported by consistent phenotypic features; surgical treatment should be favored for solitary, well limited lesions; new triazoles may be used in case of extensive lesions, especially in immunocompromised patients.
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infections cutanees a Coelomycetes en france 2005 2014
Annales De Dermatologie Et De Venereologie, 2014Co-Authors: Sarah Guégan, D Garciahermoso, K Sitbon, O LortholaryAbstract:Introduction Les phaeohyphomycoses sont des infections chroniques cosmopolites, le plus souvent cutanees, dues a des champignons filamenteux melanises, ubiquitaires et parasites des plantes. Les agents infectieux le plus frequemment rapportes sont Exophiala spp., Alternaria spp. et Phialophora spp. Les Coelomycetes, plus rarement en cause, sont un ensemble heterogene de champignons, caracterises sur le plan morphologique par leur capacite a produire des conidies (spores asexuees) a l’interieur de structures fermees ou semi-fermees visibles en culture. Materiel et methodes Tous les cas d’infections cutanees primitives a Coelomycetes envoyes pour identification au Centre national de reference mycoses invasives et antifongiques (CNRMA) de 2005 a 2014 ont ete recenses. Les donnees cliniques, histologiques et mycologiques ont ete colligees. Les differents genres et especes de Coelomycetes ont ete identifies par analyse morphologique et par sequencage des regions ITS de l’ADN ribosomique. L’etude de la sensibilite aux antifongiques a ete realisee par la methode EUCAST. Observations Dix-sept cas ont ete analyses. Resultats L’âge median etait de 60 ans. Les patients etaient, dans la tres grande majorite des cas, originaires de zones tropicales ou subtropicales. Une immunosuppression sous-jacente etait presente dans 60 % des cas. Les lesions cutanees concernaient les membres, notamment les zones distales, et etaient a types de kyste ou d’abces sous-cutanes, de plaques infiltrees, parfois hyperkeratosiques, squameuses ou necrotiques. Plus rarement, une infiltration sous-cutanee etendue etait rapportee. La recherche d’une localisation viscerale etait negative. Les Coelomycetes identifies comprenaient Medicopsis romeroi (4), Phoma herbarum (1), P. sorghina (2), P. glomerata (1), Paraconiothyrium spp. (1) et Lasiodiplodia spp. (2). Le traitement des lesions reposait sur l’exerese chirurgicale complete (7) ou partielle (1) des lesions et/ou l’emploi de traitements antifongiques par voie generale, notamment voriconazole (3) et posaconazole (3). Discussion L’analyse de la litterature depuis 1970 met en evidence moins d’une trentaine d’autres cas de phaeohyphomycoses cutanees a Coelomycetes. Il s’agit de la premiere etude rassemblant tous les cas de telles infections a l’echelle d’un pays. L’identification par sequencage est cruciale pour le diagnostic de genre et d’espece. Le traitement chirurgical par excision est a privilegier pour les lesions uniques et bien circonscrites. Dans les lesions plus etendues et chez les patients immunodeprimes, les nouveaux azoles (voriconazole, posaconazole) permettent le controle de l’infection, sans recidive. Conclusion Du fait du nombre croissant de patients immunodeprimes, les Coelomycetes sont une cause de phaeohyphomycose cutanee dont l’incidence va probablement augmenter, notamment chez les transplantes d’organes originaires de, ou ayant voyage en zone tropicale.
French Mycosis Study Group - One of the best experts on this subject based on the ideXlab platform.
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Ten-Year Experience of Cutaneous and/or Subcutaneous Infections Due to Coelomycetes in France
Open Forum Infectious Diseases, 2016Co-Authors: Sarah Guégan, Dea Garcia-hermoso, Karine Sitbon, Sarah Ahmed, Philippe Moguelet, Françoise Dromer, Olivier Lortholary, French Mycosis Study GroupAbstract:Background. Coelomycetes are rarely but increasingly reported in association with human infections involving mostly skin and subcutaneous tissues, both in immunocompetent and immunocompromised patients. Coelomycetes constitute a heterogeneous group of filamentous fungi with distinct morphological characteristics in culture, namely an ability to produce asexual spores within fruit bodies. Methods. We included all cases of proven primary cutaneous and/or subcutaneous infections due to Coelomycetes received for identification at the French National Reference Center for Invasive Mycoses and Antifungals between 2005 and 2014. Eumycetoma, chromoblastomycosis, and disseminated infections were excluded. Results. Eighteen cases were analyzed. The median age was 60.5 years. In all cases, patients originated from tropical or subtropical areas. An underlying immunodepression was present in 89% of cases. Cutaneous and/or subcutaneous lesions, mainly nodules, abscesses, or infiltrated plaques, were observed in distal body areas. Isolates of different genera of Coelomycetes were identified: Medicopsis (6), Paraconiothyrium (3), Gloniopsis (3), Diaporthe (3), Peyronellaea (2), Lasiodiplodia (1). Lesion treatment consisted of complete (10) or partial (2) surgical excision and/or the use of systemic antifungal therapy, namely voriconazole (5) and posaconazole (4). Literature review yielded 48 additional cases of cutaneous and/or subcutaneous infections due to Coelomycetes. Conclusions. Infectious diseases physicians should suspect Coelomycetes when observing cutaneous and/or subcutaneous infections in immunocompromised hosts from tropical areas; a sequence-based approach is crucial for strains identification but must be supported by consistent phenotypic features; surgical treatment should be favored for solitary, well limited lesions; new triazoles may be used in case of extensive lesions, especially in immunocompromised patients.
Françoise Dromer - One of the best experts on this subject based on the ideXlab platform.
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Ten-Year Experience of Cutaneous and/or Subcutaneous Infections Due to Coelomycetes in France
Open Forum Infectious Diseases, 2016Co-Authors: Sarah Guégan, Dea Garcia-hermoso, Karine Sitbon, Sarah Ahmed, Philippe Moguelet, Françoise Dromer, Olivier Lortholary, French Mycosis Study GroupAbstract:Background. Coelomycetes are rarely but increasingly reported in association with human infections involving mostly skin and subcutaneous tissues, both in immunocompetent and immunocompromised patients. Coelomycetes constitute a heterogeneous group of filamentous fungi with distinct morphological characteristics in culture, namely an ability to produce asexual spores within fruit bodies. Methods. We included all cases of proven primary cutaneous and/or subcutaneous infections due to Coelomycetes received for identification at the French National Reference Center for Invasive Mycoses and Antifungals between 2005 and 2014. Eumycetoma, chromoblastomycosis, and disseminated infections were excluded. Results. Eighteen cases were analyzed. The median age was 60.5 years. In all cases, patients originated from tropical or subtropical areas. An underlying immunodepression was present in 89% of cases. Cutaneous and/or subcutaneous lesions, mainly nodules, abscesses, or infiltrated plaques, were observed in distal body areas. Isolates of different genera of Coelomycetes were identified: Medicopsis (6), Paraconiothyrium (3), Gloniopsis (3), Diaporthe (3), Peyronellaea (2), Lasiodiplodia (1). Lesion treatment consisted of complete (10) or partial (2) surgical excision and/or the use of systemic antifungal therapy, namely voriconazole (5) and posaconazole (4). Literature review yielded 48 additional cases of cutaneous and/or subcutaneous infections due to Coelomycetes. Conclusions. Infectious diseases physicians should suspect Coelomycetes when observing cutaneous and/or subcutaneous infections in immunocompromised hosts from tropical areas; a sequence-based approach is crucial for strains identification but must be supported by consistent phenotypic features; surgical treatment should be favored for solitary, well limited lesions; new triazoles may be used in case of extensive lesions, especially in immunocompromised patients.
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ten year experience of cutaneous and or subcutaneous infections due to Coelomycetes in france
Open Forum Infectious Diseases, 2016Co-Authors: Sarah Guégan, Karine Sitbon, Philippe Moguelet, Françoise Dromer, Dea Garciahermoso, Sarah A Ahmed, Olivier LortholaryAbstract:Background. Coelomycetes are rarely but increasingly reported in association with human infections involving mostly skin and subcutaneous tissues, both in immunocompetent and immunocompromised patients. Coelomycetes constitute a heterogeneous group of filamentous fungi with distinct morphological characteristics in culture, namely an ability to produce asexual spores within fruit bodies. Methods. We included all cases of proven primary cutaneous and/or subcutaneous infections due to Coelomycetes received for identification at the French National Reference Center for Invasive Mycoses and Antifungals between 2005 and 2014. Eumycetoma, chromoblastomycosis, and disseminated infections were excluded. Results. Eighteen cases were analyzed. The median age was 60.5 years. In all cases, patients originated from tropical or subtropical areas. An underlying immunodepression was present in 89% of cases. Cutaneous and/or subcutaneous lesions, mainly nodules, abscesses, or infiltrated plaques, were observed in distal body areas. Isolates of different genera of Coelomycetes were identified: Medicopsis (6), Paraconiothyrium (3), Gloniopsis (3), Diaporthe (3), Peyronellaea (2), Lasiodiplodia (1). Lesion treatment consisted of complete (10) or partial (2) surgical excision and/or the use of systemic antifungal therapy, namely voriconazole (5) and posaconazole (4). Literature review yielded 48 additional cases of cutaneous and/or subcutaneous infections due to Coelomycetes. Conclusions. Infectious diseases physicians should suspect Coelomycetes when observing cutaneous and/or subcutaneous infections in immunocompromised hosts from tropical areas; a sequence-based approach is crucial for strains identification but must be supported by consistent phenotypic features; surgical treatment should be favored for solitary, well limited lesions; new triazoles may be used in case of extensive lesions, especially in immunocompromised patients.