The Experts below are selected from a list of 273 Experts worldwide ranked by ideXlab platform
Guangyou Yang - One of the best experts on this subject based on the ideXlab platform.
-
MOESM1 of Molecular characterisation and expression analysis of two heat-shock proteins in Taenia multiceps
2019Co-Authors: Yuchen Liu, Weimin Lai, Xuerong Peng, Yue Xie, Cheng Guo, Xiaowei Dong, Guangyou YangAbstract:Additional file 1: Figure S1. Western blot of rTm-p36 with six Coenurus cerebralis-positive sera. Lane M, protein molecular weight markers; Lane C, western blot of rTm-p36 (10 Âľg) with negative control goat serum
-
Characterization of glutathione S-transferase and its immunodiagnostic potential for detecting Taenia multiceps.
Veterinary parasitology, 2017Co-Authors: Ying Sun, Xing Huang, Yu Wang, Weimin Lai, Xuerong Peng, Guangyou YangAbstract:Abstract Taenia multiceps is a widespread zoonotic tapeworm parasite which infects cloven-hoofed animals around the world. Animal infection with Coenurus cerebralis, the Coenurus larvae of T. multiceps (Tm), is often fatal, which is a major cause of economic losses in stockbreeding. This study amplified the glutathione S-transferase (GST) gene from the total RNA of C. cerebralis. The resulting protein, Tm-GST, consisted of 201 amino acids, and had a predicted molecular mass of 23.1 kDa. Its amino acid sequence shares 77.61% similarity with Echinococcus granulosus GST. Recombinant Tm-GST (rTm-GST) was expressed in Escherichia coli. The protein reacted with serum from goats infected with T. multiceps. Immunofluorescence signals indicated that Tm-GST was largely localized in the parenchymatous area of adult T. multiceps; in addition, it was also apparent in the Coenurus. An enzyme-linked immunosorbent assay based on rTm-GST showed specificity of 92.8% (13/14) and sensitivity of 90% (18/20) in detecting anti-GST antibodies in serum from naturally infected animals. This study suggests that Tm-GST has the potential to be used as a diagnostic antigen for Coenurosis.
-
GP50 as a promising early diagnostic antigen for Taenia multiceps infection in goats by indirect ELISA.
Parasites & vectors, 2016Co-Authors: Xing Huang, Lin Chen, Yu Wang, Weimin Lai, Xuerong Peng, Cheng Guo, Guangyou YangAbstract:Background Coenurosis is caused by Coenurus, the metacestode of Taenia multiceps, which mainly parasitizes the brain and spinal cord of cattle, sheep and goats. To date, no widely-approved methods are available to identify early Coenurus infection.
-
Expression, tissue localization and serodiagnostic potential of Taenia multiceps acidic ribosomal protein P2.
Parasites & Vectors, 2015Co-Authors: Xing Huang, Lin Chen, Yingdong Yang, Yu Wang, Weimin Lai, Xuerong Peng, Guangyou YangAbstract:The larval stage of Taenia multiceps, also known as Coenurus, is the causative agent of coenurosis, which results in severe health problems in sheep, goats, cattle and other animals that negatively impact on animal husbandry. There is no reliable method to identify Coenurus infected goats in the early period of infection. We identified a full-length cDNA that encodes acidic ribosomal protein P2 from the transcriptome of T. multiceps (TmP2). Following cloning, sequencing and structural analyses were performed using bioinformatics tools. Recombinant TmP2 (rTmP2) was prokaryotically expressed and then used to test immunoreactivity and immunogenicity in immunoblotting assays. The native proteins in adult stage and Coenurus were located via immunofluorescence assays, while the potential of rTmP2 for indirect ELISA-based serodiagnostics was assessed using native goat sera. In addition, 20 goats were randomly divided into a drug treatment group and a control group. Each goat was orally given mature, viable T. multiceps eggs. The drug treatment group was given 10 % praziquantel by intramuscular injection 45 days post-infection (p.i), and all goats were screened for anti-TmP2 antibodies with the indirect ELISA method established here, once a week for 17 weeks p.i. The open reading frame (366 bp) of the target gene encodes a 12.62 kDa protein, which showed high homology to that from Taenia solium (93 % identity) and lacked a signal peptide. Immunofluorescence staining showed that TmP2 was highly localized to the parenchymatous zone of both the adult parasite and the Coenurus; besides, it was widely distributed in cystic wall of Coenurus. Building on good immunogenic properties, rTmP2-based ELISA exhibited a sensitivity of 95.0 % (19/20) and a specificity of 96.3 % (26/27) in detecting anti-P2 antibodies in the sera of naturally infected goats and sheep. In goats experimentally infected with T. multiceps, anti-TmP2 antibody was detectable in the control group from 3 to 10 weeks and 15 to 17 weeks p.i. In the drug-treated group, the anti-TmP2 antibody dropped below the cut-off value about 2 weeks after treatment with praziquantel and remained below this critical value until the end of the experiment. The indirect ELISA method developed in this study has the potential for detection of T. multiceps infections in hosts.
Xing Huang - One of the best experts on this subject based on the ideXlab platform.
-
Molecular cloing and bioinformatics analysis of lactate dehydrogenase from Taenia multiceps.
Parasitology research, 2017Co-Authors: Cheng Guo, Xing Huang, Yu Wang, Weimin Lai, Yue Xie, Ning Wang, Ming Yan, Bo JingAbstract:Coenurus cerebralis, the larval stage (metacestode or Coenurus) of Taenia multiceps, parasitizes sheep, goats, and other ruminants and causes coenurosis. In this study, we isolated and characterized complementary DNAs that encode lactate dehydrogenase A (Tm-LDHA) and B (Tm-LDHB) from the transcriptome of T. multiceps and expressed recombinant Tm-LDHB (rTm-LDHB) in Escherichia coli. Bioinformatic analysis showed that both Tm-LDH genes (LDHA and LDHB) contain a 996-bp open reading frame and encode a protein of 331 amino acids. After determination of the immunogenicity of the recombinant Tm-LDHB, an indirect enzyme-linked immunosorbent assay (ELISA) was developed for preliminary evaluation of the serodiagnostic potential of rTm-LDHB in goats. However, the rTm-LDHB-based indirect ELISA developed here exhibited specificity of only 71.42% (10/14) and sensitivity of 1:3200 in detection of goats infected with T. multiceps in the field. This study is the first to describe LDHA and LDHB of T. multiceps; meanwhile, our results indicate that rTm-LDHB is not a specific antigen candidate for immunodiagnosis of T. multiceps infection in goats.
-
Characterization of glutathione S-transferase and its immunodiagnostic potential for detecting Taenia multiceps.
Veterinary parasitology, 2017Co-Authors: Ying Sun, Xing Huang, Yu Wang, Weimin Lai, Xuerong Peng, Guangyou YangAbstract:Abstract Taenia multiceps is a widespread zoonotic tapeworm parasite which infects cloven-hoofed animals around the world. Animal infection with Coenurus cerebralis, the Coenurus larvae of T. multiceps (Tm), is often fatal, which is a major cause of economic losses in stockbreeding. This study amplified the glutathione S-transferase (GST) gene from the total RNA of C. cerebralis. The resulting protein, Tm-GST, consisted of 201 amino acids, and had a predicted molecular mass of 23.1 kDa. Its amino acid sequence shares 77.61% similarity with Echinococcus granulosus GST. Recombinant Tm-GST (rTm-GST) was expressed in Escherichia coli. The protein reacted with serum from goats infected with T. multiceps. Immunofluorescence signals indicated that Tm-GST was largely localized in the parenchymatous area of adult T. multiceps; in addition, it was also apparent in the Coenurus. An enzyme-linked immunosorbent assay based on rTm-GST showed specificity of 92.8% (13/14) and sensitivity of 90% (18/20) in detecting anti-GST antibodies in serum from naturally infected animals. This study suggests that Tm-GST has the potential to be used as a diagnostic antigen for Coenurosis.
-
GP50 as a promising early diagnostic antigen for Taenia multiceps infection in goats by indirect ELISA.
Parasites & vectors, 2016Co-Authors: Xing Huang, Lin Chen, Yu Wang, Weimin Lai, Xuerong Peng, Cheng Guo, Guangyou YangAbstract:Background Coenurosis is caused by Coenurus, the metacestode of Taenia multiceps, which mainly parasitizes the brain and spinal cord of cattle, sheep and goats. To date, no widely-approved methods are available to identify early Coenurus infection.
-
Expression, tissue localization and serodiagnostic potential of Taenia multiceps acidic ribosomal protein P2.
Parasites & Vectors, 2015Co-Authors: Xing Huang, Lin Chen, Yingdong Yang, Yu Wang, Weimin Lai, Xuerong Peng, Guangyou YangAbstract:The larval stage of Taenia multiceps, also known as Coenurus, is the causative agent of coenurosis, which results in severe health problems in sheep, goats, cattle and other animals that negatively impact on animal husbandry. There is no reliable method to identify Coenurus infected goats in the early period of infection. We identified a full-length cDNA that encodes acidic ribosomal protein P2 from the transcriptome of T. multiceps (TmP2). Following cloning, sequencing and structural analyses were performed using bioinformatics tools. Recombinant TmP2 (rTmP2) was prokaryotically expressed and then used to test immunoreactivity and immunogenicity in immunoblotting assays. The native proteins in adult stage and Coenurus were located via immunofluorescence assays, while the potential of rTmP2 for indirect ELISA-based serodiagnostics was assessed using native goat sera. In addition, 20 goats were randomly divided into a drug treatment group and a control group. Each goat was orally given mature, viable T. multiceps eggs. The drug treatment group was given 10 % praziquantel by intramuscular injection 45 days post-infection (p.i), and all goats were screened for anti-TmP2 antibodies with the indirect ELISA method established here, once a week for 17 weeks p.i. The open reading frame (366 bp) of the target gene encodes a 12.62 kDa protein, which showed high homology to that from Taenia solium (93 % identity) and lacked a signal peptide. Immunofluorescence staining showed that TmP2 was highly localized to the parenchymatous zone of both the adult parasite and the Coenurus; besides, it was widely distributed in cystic wall of Coenurus. Building on good immunogenic properties, rTmP2-based ELISA exhibited a sensitivity of 95.0 % (19/20) and a specificity of 96.3 % (26/27) in detecting anti-P2 antibodies in the sera of naturally infected goats and sheep. In goats experimentally infected with T. multiceps, anti-TmP2 antibody was detectable in the control group from 3 to 10 weeks and 15 to 17 weeks p.i. In the drug-treated group, the anti-TmP2 antibody dropped below the cut-off value about 2 weeks after treatment with praziquantel and remained below this critical value until the end of the experiment. The indirect ELISA method developed in this study has the potential for detection of T. multiceps infections in hosts.
Weimin Lai - One of the best experts on this subject based on the ideXlab platform.
-
MOESM1 of Molecular characterisation and expression analysis of two heat-shock proteins in Taenia multiceps
2019Co-Authors: Yuchen Liu, Weimin Lai, Xuerong Peng, Yue Xie, Cheng Guo, Xiaowei Dong, Guangyou YangAbstract:Additional file 1: Figure S1. Western blot of rTm-p36 with six Coenurus cerebralis-positive sera. Lane M, protein molecular weight markers; Lane C, western blot of rTm-p36 (10 Âľg) with negative control goat serum
-
Molecular cloing and bioinformatics analysis of lactate dehydrogenase from Taenia multiceps.
Parasitology research, 2017Co-Authors: Cheng Guo, Xing Huang, Yu Wang, Weimin Lai, Yue Xie, Ning Wang, Ming Yan, Bo JingAbstract:Coenurus cerebralis, the larval stage (metacestode or Coenurus) of Taenia multiceps, parasitizes sheep, goats, and other ruminants and causes coenurosis. In this study, we isolated and characterized complementary DNAs that encode lactate dehydrogenase A (Tm-LDHA) and B (Tm-LDHB) from the transcriptome of T. multiceps and expressed recombinant Tm-LDHB (rTm-LDHB) in Escherichia coli. Bioinformatic analysis showed that both Tm-LDH genes (LDHA and LDHB) contain a 996-bp open reading frame and encode a protein of 331 amino acids. After determination of the immunogenicity of the recombinant Tm-LDHB, an indirect enzyme-linked immunosorbent assay (ELISA) was developed for preliminary evaluation of the serodiagnostic potential of rTm-LDHB in goats. However, the rTm-LDHB-based indirect ELISA developed here exhibited specificity of only 71.42% (10/14) and sensitivity of 1:3200 in detection of goats infected with T. multiceps in the field. This study is the first to describe LDHA and LDHB of T. multiceps; meanwhile, our results indicate that rTm-LDHB is not a specific antigen candidate for immunodiagnosis of T. multiceps infection in goats.
-
Characterization of glutathione S-transferase and its immunodiagnostic potential for detecting Taenia multiceps.
Veterinary parasitology, 2017Co-Authors: Ying Sun, Xing Huang, Yu Wang, Weimin Lai, Xuerong Peng, Guangyou YangAbstract:Abstract Taenia multiceps is a widespread zoonotic tapeworm parasite which infects cloven-hoofed animals around the world. Animal infection with Coenurus cerebralis, the Coenurus larvae of T. multiceps (Tm), is often fatal, which is a major cause of economic losses in stockbreeding. This study amplified the glutathione S-transferase (GST) gene from the total RNA of C. cerebralis. The resulting protein, Tm-GST, consisted of 201 amino acids, and had a predicted molecular mass of 23.1 kDa. Its amino acid sequence shares 77.61% similarity with Echinococcus granulosus GST. Recombinant Tm-GST (rTm-GST) was expressed in Escherichia coli. The protein reacted with serum from goats infected with T. multiceps. Immunofluorescence signals indicated that Tm-GST was largely localized in the parenchymatous area of adult T. multiceps; in addition, it was also apparent in the Coenurus. An enzyme-linked immunosorbent assay based on rTm-GST showed specificity of 92.8% (13/14) and sensitivity of 90% (18/20) in detecting anti-GST antibodies in serum from naturally infected animals. This study suggests that Tm-GST has the potential to be used as a diagnostic antigen for Coenurosis.
-
GP50 as a promising early diagnostic antigen for Taenia multiceps infection in goats by indirect ELISA.
Parasites & vectors, 2016Co-Authors: Xing Huang, Lin Chen, Yu Wang, Weimin Lai, Xuerong Peng, Cheng Guo, Guangyou YangAbstract:Background Coenurosis is caused by Coenurus, the metacestode of Taenia multiceps, which mainly parasitizes the brain and spinal cord of cattle, sheep and goats. To date, no widely-approved methods are available to identify early Coenurus infection.
-
Expression, tissue localization and serodiagnostic potential of Taenia multiceps acidic ribosomal protein P2.
Parasites & Vectors, 2015Co-Authors: Xing Huang, Lin Chen, Yingdong Yang, Yu Wang, Weimin Lai, Xuerong Peng, Guangyou YangAbstract:The larval stage of Taenia multiceps, also known as Coenurus, is the causative agent of coenurosis, which results in severe health problems in sheep, goats, cattle and other animals that negatively impact on animal husbandry. There is no reliable method to identify Coenurus infected goats in the early period of infection. We identified a full-length cDNA that encodes acidic ribosomal protein P2 from the transcriptome of T. multiceps (TmP2). Following cloning, sequencing and structural analyses were performed using bioinformatics tools. Recombinant TmP2 (rTmP2) was prokaryotically expressed and then used to test immunoreactivity and immunogenicity in immunoblotting assays. The native proteins in adult stage and Coenurus were located via immunofluorescence assays, while the potential of rTmP2 for indirect ELISA-based serodiagnostics was assessed using native goat sera. In addition, 20 goats were randomly divided into a drug treatment group and a control group. Each goat was orally given mature, viable T. multiceps eggs. The drug treatment group was given 10 % praziquantel by intramuscular injection 45 days post-infection (p.i), and all goats were screened for anti-TmP2 antibodies with the indirect ELISA method established here, once a week for 17 weeks p.i. The open reading frame (366 bp) of the target gene encodes a 12.62 kDa protein, which showed high homology to that from Taenia solium (93 % identity) and lacked a signal peptide. Immunofluorescence staining showed that TmP2 was highly localized to the parenchymatous zone of both the adult parasite and the Coenurus; besides, it was widely distributed in cystic wall of Coenurus. Building on good immunogenic properties, rTmP2-based ELISA exhibited a sensitivity of 95.0 % (19/20) and a specificity of 96.3 % (26/27) in detecting anti-P2 antibodies in the sera of naturally infected goats and sheep. In goats experimentally infected with T. multiceps, anti-TmP2 antibody was detectable in the control group from 3 to 10 weeks and 15 to 17 weeks p.i. In the drug-treated group, the anti-TmP2 antibody dropped below the cut-off value about 2 weeks after treatment with praziquantel and remained below this critical value until the end of the experiment. The indirect ELISA method developed in this study has the potential for detection of T. multiceps infections in hosts.
Yu Wang - One of the best experts on this subject based on the ideXlab platform.
-
Molecular cloing and bioinformatics analysis of lactate dehydrogenase from Taenia multiceps.
Parasitology research, 2017Co-Authors: Cheng Guo, Xing Huang, Yu Wang, Weimin Lai, Yue Xie, Ning Wang, Ming Yan, Bo JingAbstract:Coenurus cerebralis, the larval stage (metacestode or Coenurus) of Taenia multiceps, parasitizes sheep, goats, and other ruminants and causes coenurosis. In this study, we isolated and characterized complementary DNAs that encode lactate dehydrogenase A (Tm-LDHA) and B (Tm-LDHB) from the transcriptome of T. multiceps and expressed recombinant Tm-LDHB (rTm-LDHB) in Escherichia coli. Bioinformatic analysis showed that both Tm-LDH genes (LDHA and LDHB) contain a 996-bp open reading frame and encode a protein of 331 amino acids. After determination of the immunogenicity of the recombinant Tm-LDHB, an indirect enzyme-linked immunosorbent assay (ELISA) was developed for preliminary evaluation of the serodiagnostic potential of rTm-LDHB in goats. However, the rTm-LDHB-based indirect ELISA developed here exhibited specificity of only 71.42% (10/14) and sensitivity of 1:3200 in detection of goats infected with T. multiceps in the field. This study is the first to describe LDHA and LDHB of T. multiceps; meanwhile, our results indicate that rTm-LDHB is not a specific antigen candidate for immunodiagnosis of T. multiceps infection in goats.
-
Characterization of glutathione S-transferase and its immunodiagnostic potential for detecting Taenia multiceps.
Veterinary parasitology, 2017Co-Authors: Ying Sun, Xing Huang, Yu Wang, Weimin Lai, Xuerong Peng, Guangyou YangAbstract:Abstract Taenia multiceps is a widespread zoonotic tapeworm parasite which infects cloven-hoofed animals around the world. Animal infection with Coenurus cerebralis, the Coenurus larvae of T. multiceps (Tm), is often fatal, which is a major cause of economic losses in stockbreeding. This study amplified the glutathione S-transferase (GST) gene from the total RNA of C. cerebralis. The resulting protein, Tm-GST, consisted of 201 amino acids, and had a predicted molecular mass of 23.1 kDa. Its amino acid sequence shares 77.61% similarity with Echinococcus granulosus GST. Recombinant Tm-GST (rTm-GST) was expressed in Escherichia coli. The protein reacted with serum from goats infected with T. multiceps. Immunofluorescence signals indicated that Tm-GST was largely localized in the parenchymatous area of adult T. multiceps; in addition, it was also apparent in the Coenurus. An enzyme-linked immunosorbent assay based on rTm-GST showed specificity of 92.8% (13/14) and sensitivity of 90% (18/20) in detecting anti-GST antibodies in serum from naturally infected animals. This study suggests that Tm-GST has the potential to be used as a diagnostic antigen for Coenurosis.
-
GP50 as a promising early diagnostic antigen for Taenia multiceps infection in goats by indirect ELISA.
Parasites & vectors, 2016Co-Authors: Xing Huang, Lin Chen, Yu Wang, Weimin Lai, Xuerong Peng, Cheng Guo, Guangyou YangAbstract:Background Coenurosis is caused by Coenurus, the metacestode of Taenia multiceps, which mainly parasitizes the brain and spinal cord of cattle, sheep and goats. To date, no widely-approved methods are available to identify early Coenurus infection.
-
Expression, tissue localization and serodiagnostic potential of Taenia multiceps acidic ribosomal protein P2.
Parasites & Vectors, 2015Co-Authors: Xing Huang, Lin Chen, Yingdong Yang, Yu Wang, Weimin Lai, Xuerong Peng, Guangyou YangAbstract:The larval stage of Taenia multiceps, also known as Coenurus, is the causative agent of coenurosis, which results in severe health problems in sheep, goats, cattle and other animals that negatively impact on animal husbandry. There is no reliable method to identify Coenurus infected goats in the early period of infection. We identified a full-length cDNA that encodes acidic ribosomal protein P2 from the transcriptome of T. multiceps (TmP2). Following cloning, sequencing and structural analyses were performed using bioinformatics tools. Recombinant TmP2 (rTmP2) was prokaryotically expressed and then used to test immunoreactivity and immunogenicity in immunoblotting assays. The native proteins in adult stage and Coenurus were located via immunofluorescence assays, while the potential of rTmP2 for indirect ELISA-based serodiagnostics was assessed using native goat sera. In addition, 20 goats were randomly divided into a drug treatment group and a control group. Each goat was orally given mature, viable T. multiceps eggs. The drug treatment group was given 10 % praziquantel by intramuscular injection 45 days post-infection (p.i), and all goats were screened for anti-TmP2 antibodies with the indirect ELISA method established here, once a week for 17 weeks p.i. The open reading frame (366 bp) of the target gene encodes a 12.62 kDa protein, which showed high homology to that from Taenia solium (93 % identity) and lacked a signal peptide. Immunofluorescence staining showed that TmP2 was highly localized to the parenchymatous zone of both the adult parasite and the Coenurus; besides, it was widely distributed in cystic wall of Coenurus. Building on good immunogenic properties, rTmP2-based ELISA exhibited a sensitivity of 95.0 % (19/20) and a specificity of 96.3 % (26/27) in detecting anti-P2 antibodies in the sera of naturally infected goats and sheep. In goats experimentally infected with T. multiceps, anti-TmP2 antibody was detectable in the control group from 3 to 10 weeks and 15 to 17 weeks p.i. In the drug-treated group, the anti-TmP2 antibody dropped below the cut-off value about 2 weeks after treatment with praziquantel and remained below this critical value until the end of the experiment. The indirect ELISA method developed in this study has the potential for detection of T. multiceps infections in hosts.
Xuerong Peng - One of the best experts on this subject based on the ideXlab platform.
-
MOESM1 of Molecular characterisation and expression analysis of two heat-shock proteins in Taenia multiceps
2019Co-Authors: Yuchen Liu, Weimin Lai, Xuerong Peng, Yue Xie, Cheng Guo, Xiaowei Dong, Guangyou YangAbstract:Additional file 1: Figure S1. Western blot of rTm-p36 with six Coenurus cerebralis-positive sera. Lane M, protein molecular weight markers; Lane C, western blot of rTm-p36 (10 Âľg) with negative control goat serum
-
Characterization of glutathione S-transferase and its immunodiagnostic potential for detecting Taenia multiceps.
Veterinary parasitology, 2017Co-Authors: Ying Sun, Xing Huang, Yu Wang, Weimin Lai, Xuerong Peng, Guangyou YangAbstract:Abstract Taenia multiceps is a widespread zoonotic tapeworm parasite which infects cloven-hoofed animals around the world. Animal infection with Coenurus cerebralis, the Coenurus larvae of T. multiceps (Tm), is often fatal, which is a major cause of economic losses in stockbreeding. This study amplified the glutathione S-transferase (GST) gene from the total RNA of C. cerebralis. The resulting protein, Tm-GST, consisted of 201 amino acids, and had a predicted molecular mass of 23.1 kDa. Its amino acid sequence shares 77.61% similarity with Echinococcus granulosus GST. Recombinant Tm-GST (rTm-GST) was expressed in Escherichia coli. The protein reacted with serum from goats infected with T. multiceps. Immunofluorescence signals indicated that Tm-GST was largely localized in the parenchymatous area of adult T. multiceps; in addition, it was also apparent in the Coenurus. An enzyme-linked immunosorbent assay based on rTm-GST showed specificity of 92.8% (13/14) and sensitivity of 90% (18/20) in detecting anti-GST antibodies in serum from naturally infected animals. This study suggests that Tm-GST has the potential to be used as a diagnostic antigen for Coenurosis.
-
GP50 as a promising early diagnostic antigen for Taenia multiceps infection in goats by indirect ELISA.
Parasites & vectors, 2016Co-Authors: Xing Huang, Lin Chen, Yu Wang, Weimin Lai, Xuerong Peng, Cheng Guo, Guangyou YangAbstract:Background Coenurosis is caused by Coenurus, the metacestode of Taenia multiceps, which mainly parasitizes the brain and spinal cord of cattle, sheep and goats. To date, no widely-approved methods are available to identify early Coenurus infection.
-
Expression, tissue localization and serodiagnostic potential of Taenia multiceps acidic ribosomal protein P2.
Parasites & Vectors, 2015Co-Authors: Xing Huang, Lin Chen, Yingdong Yang, Yu Wang, Weimin Lai, Xuerong Peng, Guangyou YangAbstract:The larval stage of Taenia multiceps, also known as Coenurus, is the causative agent of coenurosis, which results in severe health problems in sheep, goats, cattle and other animals that negatively impact on animal husbandry. There is no reliable method to identify Coenurus infected goats in the early period of infection. We identified a full-length cDNA that encodes acidic ribosomal protein P2 from the transcriptome of T. multiceps (TmP2). Following cloning, sequencing and structural analyses were performed using bioinformatics tools. Recombinant TmP2 (rTmP2) was prokaryotically expressed and then used to test immunoreactivity and immunogenicity in immunoblotting assays. The native proteins in adult stage and Coenurus were located via immunofluorescence assays, while the potential of rTmP2 for indirect ELISA-based serodiagnostics was assessed using native goat sera. In addition, 20 goats were randomly divided into a drug treatment group and a control group. Each goat was orally given mature, viable T. multiceps eggs. The drug treatment group was given 10 % praziquantel by intramuscular injection 45 days post-infection (p.i), and all goats were screened for anti-TmP2 antibodies with the indirect ELISA method established here, once a week for 17 weeks p.i. The open reading frame (366 bp) of the target gene encodes a 12.62 kDa protein, which showed high homology to that from Taenia solium (93 % identity) and lacked a signal peptide. Immunofluorescence staining showed that TmP2 was highly localized to the parenchymatous zone of both the adult parasite and the Coenurus; besides, it was widely distributed in cystic wall of Coenurus. Building on good immunogenic properties, rTmP2-based ELISA exhibited a sensitivity of 95.0 % (19/20) and a specificity of 96.3 % (26/27) in detecting anti-P2 antibodies in the sera of naturally infected goats and sheep. In goats experimentally infected with T. multiceps, anti-TmP2 antibody was detectable in the control group from 3 to 10 weeks and 15 to 17 weeks p.i. In the drug-treated group, the anti-TmP2 antibody dropped below the cut-off value about 2 weeks after treatment with praziquantel and remained below this critical value until the end of the experiment. The indirect ELISA method developed in this study has the potential for detection of T. multiceps infections in hosts.