The Experts below are selected from a list of 48 Experts worldwide ranked by ideXlab platform

Enyong Ding - One of the best experts on this subject based on the ideXlab platform.

  • glutaraldehyde assisted synthesis of Collagen Derivative modified fe 3 tio 2 nanocomposite and their enhanced photocatalytic activity
    Applied Surface Science, 2015
    Co-Authors: Feng Xue, Enyong Ding
    Abstract:

    Abstract A unique organic–inorganic hybrid nanocomposite was designed and synthesized by chemically anchoring the cationic Collagen-based Derivatives onto the surface of Fe3+/TiO2 nanospheres for the significant enhancement in photocatalytic activity under the visible light irradiation. The NMR analysis suggested the successful fabrication of cationic Collagen-g-PDMC as grafted materials. In addition, the chemical structures, morphologies and properties of these samples were systematically characterized by Fourier transform infrared spectroscopy (FTIR), X-ray diffraction (XRD), Raman spectrum, ultra violet–visible spectroscopy (UV–vis), scanning electron microscopy (SEM), transmission electron microscopy (TEM), X-ray photoelectron spectroscopy (XPS) and photoluminescence (PL). And obtained results clearly demonstrated that Fe3+ ions diffusing into TiO2 lattice could be responsible for slightly reducing the average diameter of nanospheres to about 125 nm, promoting phase transition from anatase to rutile to some extent and extending the light harvesting range into visible region markedly. Meanwhile, the achievement that Collagen-g-PDMC molecules had been covalently immobilized onto the surface of Fe3+/TiO2 nanoparticles was also well supported by the information acquired. Furthermore, the photocatalytic activities of all the as-prepared products were carefully evaluated by adopting photocatalytic decoloration of methyl orange (MO) solution under the solar direct irradiation, and the sample CFT-3 performed the best in the photocatalytic degradation process, which was mainly attributed to the energetic synergistic effect brought about by Fe3+ ions doping and Collagen-g-PDMC molecules immobilized on the surface.

  • Glutaraldehyde assisted synthesis of Collagen Derivative modified Fe 3+ /TiO 2 nanocomposite and their enhanced photocatalytic activity
    Applied Surface Science, 2015
    Co-Authors: Feng Xue, Enyong Ding
    Abstract:

    Abstract A unique organic–inorganic hybrid nanocomposite was designed and synthesized by chemically anchoring the cationic Collagen-based Derivatives onto the surface of Fe3+/TiO2 nanospheres for the significant enhancement in photocatalytic activity under the visible light irradiation. The NMR analysis suggested the successful fabrication of cationic Collagen-g-PDMC as grafted materials. In addition, the chemical structures, morphologies and properties of these samples were systematically characterized by Fourier transform infrared spectroscopy (FTIR), X-ray diffraction (XRD), Raman spectrum, ultra violet–visible spectroscopy (UV–vis), scanning electron microscopy (SEM), transmission electron microscopy (TEM), X-ray photoelectron spectroscopy (XPS) and photoluminescence (PL). And obtained results clearly demonstrated that Fe3+ ions diffusing into TiO2 lattice could be responsible for slightly reducing the average diameter of nanospheres to about 125 nm, promoting phase transition from anatase to rutile to some extent and extending the light harvesting range into visible region markedly. Meanwhile, the achievement that Collagen-g-PDMC molecules had been covalently immobilized onto the surface of Fe3+/TiO2 nanoparticles was also well supported by the information acquired. Furthermore, the photocatalytic activities of all the as-prepared products were carefully evaluated by adopting photocatalytic decoloration of methyl orange (MO) solution under the solar direct irradiation, and the sample CFT-3 performed the best in the photocatalytic degradation process, which was mainly attributed to the energetic synergistic effect brought about by Fe3+ ions doping and Collagen-g-PDMC molecules immobilized on the surface.

Didier Levieux - One of the best experts on this subject based on the ideXlab platform.

  • Differentiation of bovine from porcine gelatines using polyclonal anti-peptide antibodies in indirect and competitive indirect ELISA.
    Journal of pharmaceutical and biomedical analysis, 2005
    Co-Authors: Annie Venien, Didier Levieux
    Abstract:

    Gelatine is a Collagen Derivative obtained from bones and hides/skin mainly from bovine and pigs. As a consequence of the outbreak of bovine spongiform encephalopathy (BSE), the use of bovine gelatine in feed, food and pharmaceutical products has been restricted by regulatory authorities. However, no method was presently available for its specific detection. The large similarity in amino-acid sequences of Collagens from different species make their immunochemical differentiation difficult when using polyclonal antibodies raised against the whole molecule [A. Venien, D. Levieux, J. Immunoassay Immunochem., in press]. To obtain bovine-specific antibodies, we immunized rabbits against putative species-specific sequences of the bovine Collagen alpha 1(I) chain. Using these antibodies, an indirect ELISA was developed to allow a quick and easy differentiation between bovine and porcine gelatines. Moreover, a competitive indirect ELISA was found suitable to detect bovine gelatine in porcine gelatine purchased from laboratory chemicals suppliers down to a dilution of 2-4 parts per 1000 with CVs ranging from 5.7 to 7.7%. When testing mixtures of the largest possible range of industrial batches of bovine and porcine gelatines (skin/hides or bones origin, acid or alkaline processes, high or low Bloom) the detection limit was down to a dilution of 8 parts per 100 bovine gelatine in porcine gelatine. These ELISAs could be routinely used by pharmaceutical and food manufacturers to secure their supply chain.

  • Differentiation of bovine from porcine gelatines using polyclonal anti-peptide antibodies in indirect and competitive indirect ELISA
    Journal of Pharmaceutical and Biomedical Analysis, 2005
    Co-Authors: Annie Venien, Didier Levieux
    Abstract:

    Gelatine is a Collagen Derivative obtained from bones and hides/skin mainly from bovine and pigs. As a consequence of the outbreak of bovine spongiform encephalopathy (BSE), the use of bovine gelatine in feed, food and pharmaceutical products has been restricted by regulatory authorities. However, no method was presently available for its specific detection. The large similarity in amino-acid sequences of Collagens from different species make their immunochemical differentiation difficult when using polyclonal antibodies raised against the whole molecule [A. Venien, D. Levieux, J. Immunoassay Immunochem., in press]. To obtain bovine-specific antibodies, we immunized rabbits against putative species-specific sequences of the bovine Collagen alpha 1 (1) chain. Using these antibodies, an indirect ELISA was developed to allow a quick and easy differentiation between bovine and porcine gelatines. Moreover, a competitive indirect ELISA was found suitable to detect bovine gelatine in porcine gelatine purchased from laboratory chemicals suppliers down to a dilution of 2-4 parts per 1000 with CVs ranging from 5.7 to 7.7%. When testing mixtures of the largest possible range of industrial batches of bovine and porcine gelatines (skin/hides or bones origin, acid or alkaline processes, high or low Bloom) the detection limit

Feng Xue - One of the best experts on this subject based on the ideXlab platform.

  • glutaraldehyde assisted synthesis of Collagen Derivative modified fe 3 tio 2 nanocomposite and their enhanced photocatalytic activity
    Applied Surface Science, 2015
    Co-Authors: Feng Xue, Enyong Ding
    Abstract:

    Abstract A unique organic–inorganic hybrid nanocomposite was designed and synthesized by chemically anchoring the cationic Collagen-based Derivatives onto the surface of Fe3+/TiO2 nanospheres for the significant enhancement in photocatalytic activity under the visible light irradiation. The NMR analysis suggested the successful fabrication of cationic Collagen-g-PDMC as grafted materials. In addition, the chemical structures, morphologies and properties of these samples were systematically characterized by Fourier transform infrared spectroscopy (FTIR), X-ray diffraction (XRD), Raman spectrum, ultra violet–visible spectroscopy (UV–vis), scanning electron microscopy (SEM), transmission electron microscopy (TEM), X-ray photoelectron spectroscopy (XPS) and photoluminescence (PL). And obtained results clearly demonstrated that Fe3+ ions diffusing into TiO2 lattice could be responsible for slightly reducing the average diameter of nanospheres to about 125 nm, promoting phase transition from anatase to rutile to some extent and extending the light harvesting range into visible region markedly. Meanwhile, the achievement that Collagen-g-PDMC molecules had been covalently immobilized onto the surface of Fe3+/TiO2 nanoparticles was also well supported by the information acquired. Furthermore, the photocatalytic activities of all the as-prepared products were carefully evaluated by adopting photocatalytic decoloration of methyl orange (MO) solution under the solar direct irradiation, and the sample CFT-3 performed the best in the photocatalytic degradation process, which was mainly attributed to the energetic synergistic effect brought about by Fe3+ ions doping and Collagen-g-PDMC molecules immobilized on the surface.

  • Glutaraldehyde assisted synthesis of Collagen Derivative modified Fe 3+ /TiO 2 nanocomposite and their enhanced photocatalytic activity
    Applied Surface Science, 2015
    Co-Authors: Feng Xue, Enyong Ding
    Abstract:

    Abstract A unique organic–inorganic hybrid nanocomposite was designed and synthesized by chemically anchoring the cationic Collagen-based Derivatives onto the surface of Fe3+/TiO2 nanospheres for the significant enhancement in photocatalytic activity under the visible light irradiation. The NMR analysis suggested the successful fabrication of cationic Collagen-g-PDMC as grafted materials. In addition, the chemical structures, morphologies and properties of these samples were systematically characterized by Fourier transform infrared spectroscopy (FTIR), X-ray diffraction (XRD), Raman spectrum, ultra violet–visible spectroscopy (UV–vis), scanning electron microscopy (SEM), transmission electron microscopy (TEM), X-ray photoelectron spectroscopy (XPS) and photoluminescence (PL). And obtained results clearly demonstrated that Fe3+ ions diffusing into TiO2 lattice could be responsible for slightly reducing the average diameter of nanospheres to about 125 nm, promoting phase transition from anatase to rutile to some extent and extending the light harvesting range into visible region markedly. Meanwhile, the achievement that Collagen-g-PDMC molecules had been covalently immobilized onto the surface of Fe3+/TiO2 nanoparticles was also well supported by the information acquired. Furthermore, the photocatalytic activities of all the as-prepared products were carefully evaluated by adopting photocatalytic decoloration of methyl orange (MO) solution under the solar direct irradiation, and the sample CFT-3 performed the best in the photocatalytic degradation process, which was mainly attributed to the energetic synergistic effect brought about by Fe3+ ions doping and Collagen-g-PDMC molecules immobilized on the surface.

Annie Venien - One of the best experts on this subject based on the ideXlab platform.

  • Differentiation of bovine from porcine gelatines using polyclonal anti-peptide antibodies in indirect and competitive indirect ELISA.
    Journal of pharmaceutical and biomedical analysis, 2005
    Co-Authors: Annie Venien, Didier Levieux
    Abstract:

    Gelatine is a Collagen Derivative obtained from bones and hides/skin mainly from bovine and pigs. As a consequence of the outbreak of bovine spongiform encephalopathy (BSE), the use of bovine gelatine in feed, food and pharmaceutical products has been restricted by regulatory authorities. However, no method was presently available for its specific detection. The large similarity in amino-acid sequences of Collagens from different species make their immunochemical differentiation difficult when using polyclonal antibodies raised against the whole molecule [A. Venien, D. Levieux, J. Immunoassay Immunochem., in press]. To obtain bovine-specific antibodies, we immunized rabbits against putative species-specific sequences of the bovine Collagen alpha 1(I) chain. Using these antibodies, an indirect ELISA was developed to allow a quick and easy differentiation between bovine and porcine gelatines. Moreover, a competitive indirect ELISA was found suitable to detect bovine gelatine in porcine gelatine purchased from laboratory chemicals suppliers down to a dilution of 2-4 parts per 1000 with CVs ranging from 5.7 to 7.7%. When testing mixtures of the largest possible range of industrial batches of bovine and porcine gelatines (skin/hides or bones origin, acid or alkaline processes, high or low Bloom) the detection limit was down to a dilution of 8 parts per 100 bovine gelatine in porcine gelatine. These ELISAs could be routinely used by pharmaceutical and food manufacturers to secure their supply chain.

  • Differentiation of bovine from porcine gelatines using polyclonal anti-peptide antibodies in indirect and competitive indirect ELISA
    Journal of Pharmaceutical and Biomedical Analysis, 2005
    Co-Authors: Annie Venien, Didier Levieux
    Abstract:

    Gelatine is a Collagen Derivative obtained from bones and hides/skin mainly from bovine and pigs. As a consequence of the outbreak of bovine spongiform encephalopathy (BSE), the use of bovine gelatine in feed, food and pharmaceutical products has been restricted by regulatory authorities. However, no method was presently available for its specific detection. The large similarity in amino-acid sequences of Collagens from different species make their immunochemical differentiation difficult when using polyclonal antibodies raised against the whole molecule [A. Venien, D. Levieux, J. Immunoassay Immunochem., in press]. To obtain bovine-specific antibodies, we immunized rabbits against putative species-specific sequences of the bovine Collagen alpha 1 (1) chain. Using these antibodies, an indirect ELISA was developed to allow a quick and easy differentiation between bovine and porcine gelatines. Moreover, a competitive indirect ELISA was found suitable to detect bovine gelatine in porcine gelatine purchased from laboratory chemicals suppliers down to a dilution of 2-4 parts per 1000 with CVs ranging from 5.7 to 7.7%. When testing mixtures of the largest possible range of industrial batches of bovine and porcine gelatines (skin/hides or bones origin, acid or alkaline processes, high or low Bloom) the detection limit

João F. Mano - One of the best experts on this subject based on the ideXlab platform.

  • GelMA/bioactive silica nanocomposite bioinks for stem cell osteogenic differentiation.
    Biofabrication, 2021
    Co-Authors: Márcia T. Tavares, Vítor M. Gaspar, Maria V. Monteiro, José Paulo S. Farinha, Carlos Baleizão, João F. Mano
    Abstract:

    Leveraging 3D bioprinting for processing stem cell-laden biomaterials has unlocked a tremendous potential for fabricating living 3D constructs for bone tissue engineering. Even though several bioinks developed to date display suitable physicochemical properties for stem cell seeding and proliferation, they generally lack the nanosized minerals present in native bone bioarchitecture. To enable the bottom-up fabrication of biomimetic 3D constructs for bioinstructing stem cells pro-osteogenic differentiation, herein we developed multi-bioactive nanocomposite bioinks that combine the organic and inorganic building blocks of bone. For the organic component gelatin methacrylate (GelMA), a photocrosslinkable denaturated Collagen Derivative used for 3D bioprinting was selected due to its rheological properties display of cell adhesion moities to which bone tissue precursors such as human bone marrow derived mesenchymal stem cells (hBM-MSCs) can attach to. The inorganic building block was formulated by incorporating mesoporous silica nanoparticles functionalized with calcium, phosphate and dexamethasone (MSNCaPDex), which previously proven to induce osteogenic differentiation. The newly formulated photocrosslinkable nanocomposite GelMA bioink incorporating MSNCaPDex nanoparticles and laden with hBM-MSCs was sucessfully processed into a 3D bioprintable construct with structural fidelity and well dispersed nanoparticles throughout the hydrogel matrix. These nanocomposite constructs could induce the deposition of apatite in vitro, thus showing attractive bioactivity properties. Viability and differentiation studies showed that hBM-MSCs remained viable and exhibited osteogenic differentiation biomarkers when incorporated in GelMA/MSNCaPDex constructs and without requiring further biochemical nor mechanical stimuli. Overall, our nanocomposite bioink has demonstrated excellent processability via extrusion bioprinting into osteogenic constructs with potential application in bone tissue repair and regeneration.