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Jerzy Pałka - One of the best experts on this subject based on the ideXlab platform.

  • The mechanism for differential effect of nelfinavir and indinavir on Collagen Metabolism in human skin fibroblasts.
    Experimental dermatology, 2019
    Co-Authors: Lukasz Szoka, Ewa Karna, Edyta Andrulewicz-botulinska, Kornelia Hlebowicz-sarat, Jerzy Pałka
    Abstract:

    The mechanism for differential effects of human immune deficiency virus protease inhibitors (HIVPIs), nelfinavir (NEL) and indinavir (IND) on Collagen Metabolism disturbances was studied in human skin fibroblasts. It has been considered that HIVPIs-dependent deregulation of Collagen biosynthesis involves prolidase (an enzyme providing proline for Collagen biosynthesis), glutamine (Gln) (a substrate for proline biosynthesis), nuclear factor-κB (NF-κB) (a transcription factor that inhibit expression of type I Collagen genes), β1 integrin receptor and Akt signalling. It was found that NEL impaired Collagen biosynthesis and the process was more pronounced in the presence of Gln, while IND stimulated Collagen biosynthesis. NEL-dependent inhibition of Collagen biosynthesis was accompanied by massive intracellular accumulation of type I Collagen, while IND slightly induced this process. This effect of NEL was reversed by ascorbic acid but not N-acetylcysteine. The mechanism for the NEL-dependent defect in Collagen Metabolism was found at the level of prolidase activity, β1 integrin signalling and NF-κB. NEL inhibited expression of β1 integrin receptor, Akt and ERK1/2 and increased expression of p65 NF-κB. However, inhibitors of p65 NF-κB did not prevent NEL-dependent inhibition of Collagen biosynthesis suggesting that this transcription factor is not involved in studied mechanism. Using PI3K inhibitor wortmannin that prevent phosphorylation of Akt revealed that NEL-dependent inhibition of Akt results in inhibition of Collagen biosynthesis. The data suggest that differential effect of NEL and IND on Collagen Metabolism involves NEL-dependent down-regulation of Akt signalling and proline availability for Collagen biosynthesis.

  • Collagen Metabolism disturbances are accompanied by an increase in prolidase activity in lung carcinoma planoepitheliale.
    International journal of experimental pathology, 2004
    Co-Authors: Ewa Karna, Arkadiusz Surażyński, Jerzy Pałka
    Abstract:

    One of the consequences of neoplastic transformation is deregulation of tissue Collagen Metabolism. Although metalloproteinases initiate the breakdown of Collagen in lung carcinoma, the final step of Collagen degradation is mediated by prolidase (E.C.3.4.13.9). We investigated whether prolidase activity could reflect disturbances of Collagen Metabolism in human lung carcinoma planoepitheliale (Ca pl.). Ten human lung Ca pl. and 10 samples of normal lung parenchyma were compared with respect to prolidase activity and expression (western immunoblot), the content of Collagen and Collagen degradation products (free and bound hydroxyproline determination), beta1 integrin subunit expression (western immunoblot) and Collagenolytic activity (zymography). An increase in Collagen content (66%, P < 0.05), free proline pool (50%, P < 0.05) and Collagenolytic activity was accompanied by a significant increase in the prolidase activity (106%, P < 0.05) and its expression in Ca pl. No differences were found between Ca pl. and the control lung tissue with respect to beta1 integrin expression. Prolidase activity may reflect disturbances in tissue Collagen Metabolism in lung Ca pl. and it may, therefore, serve as a sensitive marker of the disease.

Pechova Martina - One of the best experts on this subject based on the ideXlab platform.

  • Changes of Collagen Metabolism predict the left ventricular remodeling after myocardial infarction.
    Molecular and cellular biochemistry, 2006
    Co-Authors: Jirmar Radovan, Pelouch Vaclav, Widimsky Petr, Capek Jan, Andel Michal, Prusa Richard, Pechova Martina
    Abstract:

    To analyze the predictive value of cardiac Collagen Metabolism "in vivo" in patients with myocardial infarction (MI) treated with percutaneous coronary intervention (PCI). Forty-five patients (age 66 +/- 8.27) underwent biochemical analysis for cardiac Collagen Metabolism (groups A, B and C); 30 patients with their first MI were treated with successful PCI (group A; n = 30), group B (n = 5) were MI patients with unsuccessful PCI. Group C were patients without MI (n = 10), they underwent elective diagnostic coronary angiography only. The Collagen Metabolism was analyzed in acute and subacute MI phases by using serum blood markers: the carboxy-terminal propeptide of type I proCollagen (PICP), amino-terminal propeptide of type III proCollagen (PIIINP) and carboxy-terminal telopeptide of type I Collagen (ICTP). Furthermore, the ejection fraction (EF) and left ventricular end-diastolic volume maximal changes in the course of 6 months were measured by echocardiography. A significant increase of both PICP and PIIINP on day 4 following MI was detected. Furthermore, PICP and PIIINP level assessed on the 30th day was significantly higher in the PCI unsuccessful group versus successful group. PICP level on day 4 above 110 microg/l and PIIINP level above 4 microg/l was significantly often found in the subgroup of patients with the EF improvement less than 10% or worsening and with significant left ventricular dilatation during 6 months follow-up. Cardiac catheterization itself does not affect Collagen Metabolism. We concluded that Collagen Metabolism markers enable to study in vivo the MI healing and to predict left ventricular functional and volume changes.

  • Changes of Collagen Metabolism predict the left ventricular remodeling after myocardial infarction.
    Molecular and Cellular Biochemistry, 2006
    Co-Authors: Jirmar Radovan, Pelouch Vaclav, Widimsky Petr, Capek Jan, Andel Michal, Prusa Richard, Pechova Martina
    Abstract:

    Objectives: To analyze the predictive value of cardiac Collagen Metabolism “in vivo" in patients with myocardial infarction (MI) treated with percutaneous coronary intervention (PCI). Design: Forty-five patients (age 66 ± 8.27) underwent biochemical analysis for cardiac Collagen Metabolism (groups A, B and C); 30 patients with their first MI were treated with successful PCI (group A; n = 30), group B (n = 5) were MI patients with unsuccessful PCI. Group C were patients without MI (n = 10), they underwent elective diagnostic coronary angiography only. The Collagen Metabolism was analyzed in acute and subacute MI phases by using serum blood markers: the carboxy-terminal propeptide of type I proCollagen (PICP), amino-terminal propeptide of type III proCollagen (PIIINP) and carboxy-terminal telopeptide of type I Collagen (ICTP). Furthermore, the ejection fraction (EF) and left ventricular end-diastolic volume maximal changes in the course of 6 months were measured by echocardiography. Results: A significant increase of both PICP and PIIINP on day 4 following MI was detected. Furthermore, PICP and PIIINP level assessed on the 30th day was significantly higher in the PCI unsuccessful group versus successful group. PICP level on day 4 above 110 ug/l and PIIINP level above 4 ug/l was significantly often found in the subgroup of patients with the EF improvement less than 10% or worsening and with significant left ventricular dilatation during 6 months follow-up. Cardiac catheterization itself does not affect Collagen Metabolism. Conclusion: We concluded that Collagen Metabolism markers enable to study in vivo the MI healing and to predict left ventricular functional and volume changes.

Thomas A Luger - One of the best experts on this subject based on the ideXlab platform.

Drózdz M - One of the best experts on this subject based on the ideXlab platform.

  • Assessment of the relationship between Collagen Metabolism and selected hormonal factors in extremely obese women before and after jejunoileostomy.
    Endocrine Regulations, 1997
    Co-Authors: Zofia Ostrowska, Zwirska-korczala K, Barbara Buntner, Swietochowska E, Pardela M, Bogdan Marek, Drózdz M
    Abstract:

    The majority of obese persons have hyperinsulinemia and disturbances in the secretion of melatonin, catecholamines and pituitary, thyroid, adrenal and gonadal hormones. These hormones play an important role in the regulation of Collagen Metabolism either by acting directly or by influencing IGF-I production. This study aimed at ascertaining whether, and to what degree, the changes in the concentration of hormones listed above as well as in the concentration of sex hormone-binding globulin (SHBG) and insulin-like growth factor-I (IGF-I) affect the Metabolism of Collagen as evaluated indirectly from the measurement of propeptides of type I (PICP) and type III proCollagen (PIIINP) in blood serum and hydroxyproline in urine. The study compared 30 women with extreme obesity before and three to five years after jejunoileostomy with 20 healthy women of reproductive age. All non-operated obese women showed significantly increased concentration of serum insulin, IGF-I, melatonin, norepinephrine, free triiodothyronine, estradiol, total and free testosterone, PICP, PIIINP and urinary excretion of hydroxyproline, while the levels of epinephrine, progesterone and SHBG were significantly decreased. Changes in the level of the examined markers of Collagen Metabolism correlated positively with the concentration of insulin, IGF-I and sex hormones, while the correlation with epinephrine, cortisol and thyroid hormones was negative. All women who were treated previously by jejunoileostomy showed a decreased of body mass to regular values, normalization of hormonal disturbances and normal Collagen Metabolism. The obtained results show that the increased Collagen Metabolism observed in extremely obese women is caused indirectly by altered endocrine activity.

Gregorios Doumanis - One of the best experts on this subject based on the ideXlab platform.

  • The Relationship between Androgens, Regulators of Collagen Metabolism, and Peyronie's Disease: A Case Control Study
    The journal of sexual medicine, 2010
    Co-Authors: Markos Karavitakis, Christos Komninos, V. Simaioforidis, Stylianos Kontos, George Lefakis, Vasilios Politis, George Koritsiadis, Katerina Konstantellou, Gregorios Doumanis
    Abstract:

    ABSTRACT Introduction Changes in Collagen Metabolism have been postulated to play a pivotal role in the pathogenesis of Peyronie's Disease (PD). Androgens such as dehydroepiandrosterone sulfate (DHEA-S) and testosterone influence Collagen Metabolism by modulating the activity of matrix metalloproteases (MMP) and tissue inhibitors of metalloproteases (TIMP). Aim The aim of this study was to evaluate the interrelationship between androgens (DHEA-S and testosterone), key regulators of Collagen Metabolism such as insulin-like growth factor (IGF) 1 and IGF Binding Protein 3 (IGF-BP3), the MMP/TIMP system, and PD. Methods Age matched PD patients (14) and healthy men (10) who acted as controls were recruited. Blood samples were collected from all subjects in the early morning hours after an overnight fast. Main Outcome Measures Serum levels of testosterone, sex hormone binding globulin, DHEA-S, 3-α-androstanediol glucuronide, pro-MMP-1, MMP-1, MMP-2, TIMP-1, TIMP-2, IGF-1 and IGF-BP3 were measured in both groups. Statistical methods included univariate, bivariate, and multivariate regression models. Results Levels of DHEA-S (114.5 vs. 169.5 µg/dL; p  = 0.03), IGF-BP3 (2.96 vs. 3.79 µg/mL; p  = 0.01), and TIMP-1 (173.1 vs. 195 ng/mL; p  = 0.01) were significantly lower in PD patients. In contrast, the level of TIMP-2 (102 vs. 85 ng/mL; p  = 0.001) was significantly lower in the control group. Using stepwise regression analysis, only TIMP-2 ( p p  = 0.04) were significantly related to PD in the final model (R 2  = 0.63). TIMP-1 and DHEA-S (r = 0.55, p p p Conclusions Our findings suggest that decreased levels of adrenal androgens may be implicated in the pathogenesis of PD. The mechanism and clinical relevance of this observation remain to be established. Karavitakis M, Komninos C, Simaioforidis V, Kontos S, Lefakis G, Politis V, Koritsiadis G, Konstantellou K, and Doumanis G. The relationship between androgens, regulators of Collagen Metabolism, and peyronie's disease: A case control study.