The Experts below are selected from a list of 225 Experts worldwide ranked by ideXlab platform
Dirk Wernicke - One of the best experts on this subject based on the ideXlab platform.
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Stimulation of Collagenase 3 expression in synovial fibroblasts of patients with rheumatoid arthritis by contact with a three‐dimensional collagen matrix or with normal cartilage when coimplanted in NOD/SCID mice
Arthritis and rheumatism, 2002Co-Authors: Dirk Wernicke, P.k. Petrow, Josef Zacher, Rolf Bräuer, Claudia Schulze-westhoff, Erika Gromnica-ihleAbstract:Objective To study the expression of Collagenase 3 (matrix metalloproteinase 13 [MMP-13]) and Collagenase 1 (MMP-1) in synovial fibroblasts from patients with rheumatoid arthritis (RA) when cultured within 3-dimensional collagen gels or coimplanted with normal cartilage in immunodeficient NOD/SCID mice. Methods Messenger RNA (mRNA) and protein expression of Collagenase 3 and Collagenase 1 were characterized in synovial and skin fibroblasts by Northern blot and Western blot analysis. The mRNA expression of both Collagenases in cell–cartilage implants in NOD/SCID mice was investigated by in situ hybridization in combination with immunohistochemistry of human fibroblasts. Results Synovial fibroblasts coimplanted with normal cartilage in NOD/SCID mice deeply invaded adjacent cartilage tissue. In this in vivo system of cartilage destruction, Collagenase 3 mRNA was induced in synovial fibroblasts at sites of cartilage erosion, while the expression of Collagenase 1 mRNA could not be detected. Culture of synovial fibroblasts within 3-dimensional collagen gels was a ssociated with a marked increase in Collagenase 3 mRNA expression and proenzyme production. This stimulatory effect was 1 order of magnitude higher in comparison with a 2–4-fold increase upon treatment with interleukin-1 β or tumor necrosis factor α. In contrast, mRNA expression and proenzyme production of Collagenase 1 were increased strongly, and to a similar extent, either by contact with 3-dimensional collagen or by proinflammatory cytokines. Conclusion The expression of Collagenase 3, in contrast to that of Collagenase 1, is preferentially stimulated in synovial fibroblasts by 3-dimensional collagen rather than by proinflammatory cytokines. The induction of Collagenase 3 by cell–matrix interactions represents a potential mechanism contributing to the invasive phenotype of synovial fibroblasts at sites of synovial invasion into cartilage in RA.
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stimulation of Collagenase 3 expression in synovial fibroblasts of patients with rheumatoid arthritis by contact with a three dimensional collagen matrix or with normal cartilage when coimplanted in nod scid mice
Arthritis & Rheumatism, 2002Co-Authors: Dirk Wernicke, P.k. Petrow, Josef Zacher, Rolf Bräuer, Claudia Schulzewesthoff, Erika GromnicaihleAbstract:Objective To study the expression of Collagenase 3 (matrix metalloproteinase 13 [MMP-13]) and Collagenase 1 (MMP-1) in synovial fibroblasts from patients with rheumatoid arthritis (RA) when cultured within 3-dimensional collagen gels or coimplanted with normal cartilage in immunodeficient NOD/SCID mice. Methods Messenger RNA (mRNA) and protein expression of Collagenase 3 and Collagenase 1 were characterized in synovial and skin fibroblasts by Northern blot and Western blot analysis. The mRNA expression of both Collagenases in cell–cartilage implants in NOD/SCID mice was investigated by in situ hybridization in combination with immunohistochemistry of human fibroblasts. Results Synovial fibroblasts coimplanted with normal cartilage in NOD/SCID mice deeply invaded adjacent cartilage tissue. In this in vivo system of cartilage destruction, Collagenase 3 mRNA was induced in synovial fibroblasts at sites of cartilage erosion, while the expression of Collagenase 1 mRNA could not be detected. Culture of synovial fibroblasts within 3-dimensional collagen gels was a ssociated with a marked increase in Collagenase 3 mRNA expression and proenzyme production. This stimulatory effect was 1 order of magnitude higher in comparison with a 2–4-fold increase upon treatment with interleukin-1 β or tumor necrosis factor α. In contrast, mRNA expression and proenzyme production of Collagenase 1 were increased strongly, and to a similar extent, either by contact with 3-dimensional collagen or by proinflammatory cytokines. Conclusion The expression of Collagenase 3, in contrast to that of Collagenase 1, is preferentially stimulated in synovial fibroblasts by 3-dimensional collagen rather than by proinflammatory cytokines. The induction of Collagenase 3 by cell–matrix interactions represents a potential mechanism contributing to the invasive phenotype of synovial fibroblasts at sites of synovial invasion into cartilage in RA.
Constance E Brinckerhoff - One of the best experts on this subject based on the ideXlab platform.
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interstitial Collagenases as markers of tumor progression
Clinical Cancer Research, 2000Co-Authors: Constance E Brinckerhoff, Joni L Rutter, Ulrike BenbowAbstract:Degradation of the extracellular matrix is the sine qua non of tumor invasion and metastasis. Most of this degradation is mediated by matrix metalloproteinases (MMPs), a family of enzymes that, collectively, degrades the extracellular matrix. Although the basement membrane-degrading enzymes, MMP-2 and MMP-9, have been given considerable attention for their roles in invasion and metastasis, the interstitial Collagenases, a subfamily of MMPs that cleaves the stromal collagens types I and III, have received relatively little recognition for their part in these processes. This subfamily is comprised of Collagenase 1 (MMP-1), Collagenase 3 (MMP-13), and the MT-MMPs, membrane-bound MMPs, and numerous reports over the last several years document the expression of these MMPs in a wide variety of advancing tumors. Of particular interest is a single nucleotide polymorphism in the MMP-1 promoter that increases the transcription of this gene and that is associated with melanoma and with ovarian and endometrial cancers. The Collagenases can mediate tumor invasion through several mechanisms, which include constitutive production of enzyme by the tumor cells, induction of Collagenase production in the neighboring stromal cells, and interactions between tumor/ stromal cells to induce Collagenase production by one or both cell types. Thus, evidence indicates that elevated expression of the interstitial Collagenases is associated with a poor prognosis in a variety of cancers, and therefore, these MMPs can serve as a marker of tumor progression.
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cloning of the gene for interstitial Collagenase 3 matrix metalloproteinase 13 from rabbit synovial fibroblasts differential expression with Collagenase 1 matrix metalloproteinase 1
Biochemical Journal, 1998Co-Authors: Matthew P Vincenti, P Mitchell, Charles I Coon, John A Mengshol, S Yocum, Constance E BrinckerhoffAbstract:Cartilage, bone and the interstitial stroma, composed largely of the interstitial collagens, types I, II and III, are remodelled by three members of the metalloproteinase (MMP) family, Collagenase-1 (MMP-1), Collagenase-2 (MMP-8) and Collagenase-3 (MMP-13). MMP-1 and MMP-13 may contribute directly to disease progression, since they are induced in patients with rheumatoid arthritis and osteoarthritis. The study of MMP-1 and MMP-13 gene regulation in models of arthritic disease has been problematic because mice and rats, which are typically used, only possess a homologue of MMP-13. Here we show that in contrast with mice and rats, rabbits possess distinct genes homologous to human MMP-1 and MMP-13. Furthermore, rabbit MMP-13 is expressed simultaneously with MMP-1 in chondrocytes and synovial fibroblasts in response to the cytokines interleukin-1 and tumour necrosis factor-alpha, or the phorbol ester PMA. The time course of MMP-13 induction is more rapid and transient than that of MMP-1, suggesting that distinct mechanisms regulate the expression of these two Collagenases. We have cloned the rabbit MMP-13 gene from synovial fibroblasts and demonstrated that the rabbit gene shares greater homology with human MMP-13 than does the mouse interstitial Collagenase. Together with the fact that mice and rats do not possess a homologue to human MMP-1, our data suggest that the rabbit provides an appropriate model for studying the roles of interstitial Collagenases in connective-tissue diseases, such as rheumatoid arthritis and osteoarthritis.
Erika Gromnica-ihle - One of the best experts on this subject based on the ideXlab platform.
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Stimulation of Collagenase 3 expression in synovial fibroblasts of patients with rheumatoid arthritis by contact with a three‐dimensional collagen matrix or with normal cartilage when coimplanted in NOD/SCID mice
Arthritis and rheumatism, 2002Co-Authors: Dirk Wernicke, P.k. Petrow, Josef Zacher, Rolf Bräuer, Claudia Schulze-westhoff, Erika Gromnica-ihleAbstract:Objective To study the expression of Collagenase 3 (matrix metalloproteinase 13 [MMP-13]) and Collagenase 1 (MMP-1) in synovial fibroblasts from patients with rheumatoid arthritis (RA) when cultured within 3-dimensional collagen gels or coimplanted with normal cartilage in immunodeficient NOD/SCID mice. Methods Messenger RNA (mRNA) and protein expression of Collagenase 3 and Collagenase 1 were characterized in synovial and skin fibroblasts by Northern blot and Western blot analysis. The mRNA expression of both Collagenases in cell–cartilage implants in NOD/SCID mice was investigated by in situ hybridization in combination with immunohistochemistry of human fibroblasts. Results Synovial fibroblasts coimplanted with normal cartilage in NOD/SCID mice deeply invaded adjacent cartilage tissue. In this in vivo system of cartilage destruction, Collagenase 3 mRNA was induced in synovial fibroblasts at sites of cartilage erosion, while the expression of Collagenase 1 mRNA could not be detected. Culture of synovial fibroblasts within 3-dimensional collagen gels was a ssociated with a marked increase in Collagenase 3 mRNA expression and proenzyme production. This stimulatory effect was 1 order of magnitude higher in comparison with a 2–4-fold increase upon treatment with interleukin-1 β or tumor necrosis factor α. In contrast, mRNA expression and proenzyme production of Collagenase 1 were increased strongly, and to a similar extent, either by contact with 3-dimensional collagen or by proinflammatory cytokines. Conclusion The expression of Collagenase 3, in contrast to that of Collagenase 1, is preferentially stimulated in synovial fibroblasts by 3-dimensional collagen rather than by proinflammatory cytokines. The induction of Collagenase 3 by cell–matrix interactions represents a potential mechanism contributing to the invasive phenotype of synovial fibroblasts at sites of synovial invasion into cartilage in RA.
Erika Gromnicaihle - One of the best experts on this subject based on the ideXlab platform.
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stimulation of Collagenase 3 expression in synovial fibroblasts of patients with rheumatoid arthritis by contact with a three dimensional collagen matrix or with normal cartilage when coimplanted in nod scid mice
Arthritis & Rheumatism, 2002Co-Authors: Dirk Wernicke, P.k. Petrow, Josef Zacher, Rolf Bräuer, Claudia Schulzewesthoff, Erika GromnicaihleAbstract:Objective To study the expression of Collagenase 3 (matrix metalloproteinase 13 [MMP-13]) and Collagenase 1 (MMP-1) in synovial fibroblasts from patients with rheumatoid arthritis (RA) when cultured within 3-dimensional collagen gels or coimplanted with normal cartilage in immunodeficient NOD/SCID mice. Methods Messenger RNA (mRNA) and protein expression of Collagenase 3 and Collagenase 1 were characterized in synovial and skin fibroblasts by Northern blot and Western blot analysis. The mRNA expression of both Collagenases in cell–cartilage implants in NOD/SCID mice was investigated by in situ hybridization in combination with immunohistochemistry of human fibroblasts. Results Synovial fibroblasts coimplanted with normal cartilage in NOD/SCID mice deeply invaded adjacent cartilage tissue. In this in vivo system of cartilage destruction, Collagenase 3 mRNA was induced in synovial fibroblasts at sites of cartilage erosion, while the expression of Collagenase 1 mRNA could not be detected. Culture of synovial fibroblasts within 3-dimensional collagen gels was a ssociated with a marked increase in Collagenase 3 mRNA expression and proenzyme production. This stimulatory effect was 1 order of magnitude higher in comparison with a 2–4-fold increase upon treatment with interleukin-1 β or tumor necrosis factor α. In contrast, mRNA expression and proenzyme production of Collagenase 1 were increased strongly, and to a similar extent, either by contact with 3-dimensional collagen or by proinflammatory cytokines. Conclusion The expression of Collagenase 3, in contrast to that of Collagenase 1, is preferentially stimulated in synovial fibroblasts by 3-dimensional collagen rather than by proinflammatory cytokines. The induction of Collagenase 3 by cell–matrix interactions represents a potential mechanism contributing to the invasive phenotype of synovial fibroblasts at sites of synovial invasion into cartilage in RA.
Josef Zacher - One of the best experts on this subject based on the ideXlab platform.
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Stimulation of Collagenase 3 expression in synovial fibroblasts of patients with rheumatoid arthritis by contact with a three‐dimensional collagen matrix or with normal cartilage when coimplanted in NOD/SCID mice
Arthritis and rheumatism, 2002Co-Authors: Dirk Wernicke, P.k. Petrow, Josef Zacher, Rolf Bräuer, Claudia Schulze-westhoff, Erika Gromnica-ihleAbstract:Objective To study the expression of Collagenase 3 (matrix metalloproteinase 13 [MMP-13]) and Collagenase 1 (MMP-1) in synovial fibroblasts from patients with rheumatoid arthritis (RA) when cultured within 3-dimensional collagen gels or coimplanted with normal cartilage in immunodeficient NOD/SCID mice. Methods Messenger RNA (mRNA) and protein expression of Collagenase 3 and Collagenase 1 were characterized in synovial and skin fibroblasts by Northern blot and Western blot analysis. The mRNA expression of both Collagenases in cell–cartilage implants in NOD/SCID mice was investigated by in situ hybridization in combination with immunohistochemistry of human fibroblasts. Results Synovial fibroblasts coimplanted with normal cartilage in NOD/SCID mice deeply invaded adjacent cartilage tissue. In this in vivo system of cartilage destruction, Collagenase 3 mRNA was induced in synovial fibroblasts at sites of cartilage erosion, while the expression of Collagenase 1 mRNA could not be detected. Culture of synovial fibroblasts within 3-dimensional collagen gels was a ssociated with a marked increase in Collagenase 3 mRNA expression and proenzyme production. This stimulatory effect was 1 order of magnitude higher in comparison with a 2–4-fold increase upon treatment with interleukin-1 β or tumor necrosis factor α. In contrast, mRNA expression and proenzyme production of Collagenase 1 were increased strongly, and to a similar extent, either by contact with 3-dimensional collagen or by proinflammatory cytokines. Conclusion The expression of Collagenase 3, in contrast to that of Collagenase 1, is preferentially stimulated in synovial fibroblasts by 3-dimensional collagen rather than by proinflammatory cytokines. The induction of Collagenase 3 by cell–matrix interactions represents a potential mechanism contributing to the invasive phenotype of synovial fibroblasts at sites of synovial invasion into cartilage in RA.
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stimulation of Collagenase 3 expression in synovial fibroblasts of patients with rheumatoid arthritis by contact with a three dimensional collagen matrix or with normal cartilage when coimplanted in nod scid mice
Arthritis & Rheumatism, 2002Co-Authors: Dirk Wernicke, P.k. Petrow, Josef Zacher, Rolf Bräuer, Claudia Schulzewesthoff, Erika GromnicaihleAbstract:Objective To study the expression of Collagenase 3 (matrix metalloproteinase 13 [MMP-13]) and Collagenase 1 (MMP-1) in synovial fibroblasts from patients with rheumatoid arthritis (RA) when cultured within 3-dimensional collagen gels or coimplanted with normal cartilage in immunodeficient NOD/SCID mice. Methods Messenger RNA (mRNA) and protein expression of Collagenase 3 and Collagenase 1 were characterized in synovial and skin fibroblasts by Northern blot and Western blot analysis. The mRNA expression of both Collagenases in cell–cartilage implants in NOD/SCID mice was investigated by in situ hybridization in combination with immunohistochemistry of human fibroblasts. Results Synovial fibroblasts coimplanted with normal cartilage in NOD/SCID mice deeply invaded adjacent cartilage tissue. In this in vivo system of cartilage destruction, Collagenase 3 mRNA was induced in synovial fibroblasts at sites of cartilage erosion, while the expression of Collagenase 1 mRNA could not be detected. Culture of synovial fibroblasts within 3-dimensional collagen gels was a ssociated with a marked increase in Collagenase 3 mRNA expression and proenzyme production. This stimulatory effect was 1 order of magnitude higher in comparison with a 2–4-fold increase upon treatment with interleukin-1 β or tumor necrosis factor α. In contrast, mRNA expression and proenzyme production of Collagenase 1 were increased strongly, and to a similar extent, either by contact with 3-dimensional collagen or by proinflammatory cytokines. Conclusion The expression of Collagenase 3, in contrast to that of Collagenase 1, is preferentially stimulated in synovial fibroblasts by 3-dimensional collagen rather than by proinflammatory cytokines. The induction of Collagenase 3 by cell–matrix interactions represents a potential mechanism contributing to the invasive phenotype of synovial fibroblasts at sites of synovial invasion into cartilage in RA.