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Snežana Zdravkovickorac - One of the best experts on this subject based on the ideXlab platform.
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somatic embryogenesis and shoot organogenesis from the hypocotyl slices and free radical scavenging activity of regenerants of Collard Greens brassica oleracea l var acephala
Plant Cell Tissue and Organ Culture, 2019Co-Authors: Nevena Banjac, Branka Vinterhalter, Dijana Krsticmilosevic, Jelena Milojevic, Ljiljana Tubic, Nabil Ghalawenji, Snežana ZdravkovickoracAbstract:Brassica oleracea var. acephala is an important leafy vegetable that has been widely consumed as a high-nutrient, low-calorie food. Because of the plant’s biennial and self-incompatibility nature, biotechnological approaches are alternative way for propagation and breeding improvements. Since tissue culture studies have been focused in other B. oleracea representatives, the aim of the present study was to achieve effective regeneration protocol distinctive for Collard Greens, and evaluate the total phenolic content and antioxidant activity of regenerants. The effect of 3 cytokinins [thidiazuron (TDZ), 6-benzyladenine (BA) and 6-furfuryladenine (kinetin, KIN)] at increasing concentrations (0, 5, 7.5, 10, 20 or 30 µM) in combination with tenfold lower concentration of 1-naphtaleneacetic acid (NAA) (0, 0.5, 0.75, 1, 2 or 3 µM, respectively) on the regeneration from hypocotyl slices was studied. Histological analysis revealed the two regeneration pathways, somatic embryogenesis and shoot organogenesis, simultaneously occurred in the same explant, regardless of the cytokinin/NAA combinations used. The regeneration frequency of 95.9%, with 7.5 morphogenic structures regenerated per explant, and the healthy appearance of regenerated plants indicated the optimal combination 20 µM TDZ + 2 µM NAA. TDZ at 5 µM provided the high somatic embryo proliferation rate by generation of secondary embryos (7.79) along with the lowest rate of their abnormalities. Embryo-developed plants were successfully acclimatised (above 90%). The plants regenerated and proliferated on TDZ-containing media had higher total phenolic content that correlated with the highest free radical scavenging activity (IC50 = 19.09 µg ml− 1). Somatic embryogenesis and shoot organogenesis from hypocotyl slices of the Collard Greens were established. The combination 20 µM TDZ + 2 µM NAA was optimal for regeneration providing higher total phenolic content.
Nevena Banjac - One of the best experts on this subject based on the ideXlab platform.
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somatic embryogenesis and shoot organogenesis from the hypocotyl slices and free radical scavenging activity of regenerants of Collard Greens brassica oleracea l var acephala
Plant Cell Tissue and Organ Culture, 2019Co-Authors: Nevena Banjac, Branka Vinterhalter, Dijana Krsticmilosevic, Jelena Milojevic, Ljiljana Tubic, Nabil Ghalawenji, Snežana ZdravkovickoracAbstract:Brassica oleracea var. acephala is an important leafy vegetable that has been widely consumed as a high-nutrient, low-calorie food. Because of the plant’s biennial and self-incompatibility nature, biotechnological approaches are alternative way for propagation and breeding improvements. Since tissue culture studies have been focused in other B. oleracea representatives, the aim of the present study was to achieve effective regeneration protocol distinctive for Collard Greens, and evaluate the total phenolic content and antioxidant activity of regenerants. The effect of 3 cytokinins [thidiazuron (TDZ), 6-benzyladenine (BA) and 6-furfuryladenine (kinetin, KIN)] at increasing concentrations (0, 5, 7.5, 10, 20 or 30 µM) in combination with tenfold lower concentration of 1-naphtaleneacetic acid (NAA) (0, 0.5, 0.75, 1, 2 or 3 µM, respectively) on the regeneration from hypocotyl slices was studied. Histological analysis revealed the two regeneration pathways, somatic embryogenesis and shoot organogenesis, simultaneously occurred in the same explant, regardless of the cytokinin/NAA combinations used. The regeneration frequency of 95.9%, with 7.5 morphogenic structures regenerated per explant, and the healthy appearance of regenerated plants indicated the optimal combination 20 µM TDZ + 2 µM NAA. TDZ at 5 µM provided the high somatic embryo proliferation rate by generation of secondary embryos (7.79) along with the lowest rate of their abnormalities. Embryo-developed plants were successfully acclimatised (above 90%). The plants regenerated and proliferated on TDZ-containing media had higher total phenolic content that correlated with the highest free radical scavenging activity (IC50 = 19.09 µg ml− 1). Somatic embryogenesis and shoot organogenesis from hypocotyl slices of the Collard Greens were established. The combination 20 µM TDZ + 2 µM NAA was optimal for regeneration providing higher total phenolic content.
Sarah L Booth - One of the best experts on this subject based on the ideXlab platform.
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α tocopherol disappearance rates from plasma depend on lipid concentrations studies using deuterium labeled Collard Greens in younger and older adults
The American Journal of Clinical Nutrition, 2015Co-Authors: Maret G Traber, Scott W Leonard, Gerd Bobe, Edward Saltzman, Michael A Grusak, Sarah L BoothAbstract:Background: Little is known about α-tocopherol’s bioavailability as a constituent of food or its dependence on a subject’s age. Objective: To evaluate the α-tocopherol bioavailability from food, we used Collard Greens grown in deuterated water (2H Collard Greens) as a source of deuterium-labeled (2H) α-tocopherol consumed by younger and older adults in a post hoc analysis of a vitamin K study. Design: Younger (mean ± SD age: 32 ± 7 y; n = 12 women and 9 men) and older (aged 67 ± 8 y; n = 8 women and 12 men) adults consumed a test breakfast that included 120 g 2H Collard Greens (1.2 ± 0.1 mg 2H-α-tocopherol). Plasma unlabeled α-tocopherol and 2H-α-tocopherol were measured by using liquid chromatography–mass spectrometry from fasting (>12 h) blood samples drawn before breakfast (0 h) and at 24, 48, and 72 h and from postprandial samples collected at 4, 5, 6, 7, 9, 12, and 16 h. Results: Times (12.6 ± 2.5 h) of maximum plasma 2H-α-tocopherol concentrations (0.82% ± 0.59% total α-tocopherol), fractional disappearance rates (0.63 ± 0.26 pools/d), half-lives (30 ± 11 h), and the minimum estimated 2H-α-tocopherol absorbed (24% ± 16%) did not vary between age groups or sexes (n = 41). Unlabeled α-tocopherol concentrations were higher in older adults (26.4 ± 8.6 μmol/L) than in younger adults (19.3 ± 4.2 μmol/L; P = 0.0019) and correlated with serum lipids (r = 0.4938, P = 0.0012). In addition, 2H-α-tocopherol half-lives were correlated with lipids (r = 0.4361, P = 0.0044). Conclusions: Paradoxically, α-tocopherol remained in circulation longer in participants with higher serum lipids, but the 2H-α-tocopherol absorbed was not dependent on the plasma lipid status. Neither variable was dependent on age. These data suggest that plasma α-tocopherol concentrations are more dependent on mechanisms that control circulating lipids rather than those related to its absorption and initial incorporation into plasma. This trial was registered at clinicaltrials.gov as NCT0036232.
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a tocopherol disappearance rates from plasma depend on lipid concentrations studies using deuterium labeled Collard Greens in
2015Co-Authors: Maret G Traber, Scott W Leonard, Gerd Bobe, Edward Saltzman, Michael A Grusak, Sarah L BoothAbstract:Background: Little is known about a-tocopherol’s bioavailability as a constituent of food or its dependence on a subject’s age. Objective: To evaluate the a-tocopherol bioavailability from food, we used Collard Greens grown in deuterated water ( 2 H Collard Greens) as a source of deuterium-labeled ( 2 H) a-tocopherol consumed by younger and older adults in a post hoc analysis of a vitamin K study. Design: Younger (mean 6 SD age: 32 6 7y ;n = 12 women and 9 men) and older (aged 67 6 8y ;n = 8 women and 12 men) adults consumed a test breakfast that included 120 g 2 H Collard Greens (1.2 6 0.1 mg 2 H-a-tocopherol). Plasma unlabeled a-tocopherol and 2 H-a-tocopherol were measured by using liquid chromatography–
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plasma transport of vitamin k in men using deuterium labeled Collard Greens
Metabolism-clinical and Experimental, 2004Co-Authors: Arja T Erkkila, Michael A Grusak, Alice H Lichtenstein, Gregory G Dolnikowski, Susan M Jalbert, Katherine A Aquino, James W Peterson, Sarah L BoothAbstract:The plasma transport of stable isotope-labeled phylloquinone at physiologic doses from food was studied. A single bolus of 100 g (396 +/- 28 microg phylloquinone) deuterium-labeled Collard Greens was fed with a breakfast containing 24 g fat to 5 men (26 to 71 years). Eleven blood samples were obtained over 216 hours. Phylloquinone concentrations in plasma and lipoprotein subfractions were measured using high-performance liquid chromatography (HPLC), and the ion abundances of deuterated and endogenous phylloquinone were determined using gas chromatography/mass spectrometry (GC/MS). Plasma total phylloquinone concentrations peaked at 6 to 9 hours (10.51 +/- 4.38 to 8.30 +/- 4.64 nmol/L) and returned to baseline by 24 hours (1.26 +/- 0.38 nmol/L). The triglyceride-rich lipoprotein (TRL) fraction was the major carrier of phylloquinone; low-density lipoprotein (LDL) and high-density lipoprotein (HDL) fractions contained smaller amounts. Maximum enrichment of plasma and TRL phylloquinone with deuterium (88% and 89%, respectively) was reached at 6 hours, respectively; t(1/2) was 22.8 hours (n = 3). Deuterated-phylloquinone was not detectable in plasma or TRL fraction at 72 hours. These results suggest rapid uptake and transport of physiologic doses of phylloquinone.
Nabil Ghalawenji - One of the best experts on this subject based on the ideXlab platform.
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somatic embryogenesis and shoot organogenesis from the hypocotyl slices and free radical scavenging activity of regenerants of Collard Greens brassica oleracea l var acephala
Plant Cell Tissue and Organ Culture, 2019Co-Authors: Nevena Banjac, Branka Vinterhalter, Dijana Krsticmilosevic, Jelena Milojevic, Ljiljana Tubic, Nabil Ghalawenji, Snežana ZdravkovickoracAbstract:Brassica oleracea var. acephala is an important leafy vegetable that has been widely consumed as a high-nutrient, low-calorie food. Because of the plant’s biennial and self-incompatibility nature, biotechnological approaches are alternative way for propagation and breeding improvements. Since tissue culture studies have been focused in other B. oleracea representatives, the aim of the present study was to achieve effective regeneration protocol distinctive for Collard Greens, and evaluate the total phenolic content and antioxidant activity of regenerants. The effect of 3 cytokinins [thidiazuron (TDZ), 6-benzyladenine (BA) and 6-furfuryladenine (kinetin, KIN)] at increasing concentrations (0, 5, 7.5, 10, 20 or 30 µM) in combination with tenfold lower concentration of 1-naphtaleneacetic acid (NAA) (0, 0.5, 0.75, 1, 2 or 3 µM, respectively) on the regeneration from hypocotyl slices was studied. Histological analysis revealed the two regeneration pathways, somatic embryogenesis and shoot organogenesis, simultaneously occurred in the same explant, regardless of the cytokinin/NAA combinations used. The regeneration frequency of 95.9%, with 7.5 morphogenic structures regenerated per explant, and the healthy appearance of regenerated plants indicated the optimal combination 20 µM TDZ + 2 µM NAA. TDZ at 5 µM provided the high somatic embryo proliferation rate by generation of secondary embryos (7.79) along with the lowest rate of their abnormalities. Embryo-developed plants were successfully acclimatised (above 90%). The plants regenerated and proliferated on TDZ-containing media had higher total phenolic content that correlated with the highest free radical scavenging activity (IC50 = 19.09 µg ml− 1). Somatic embryogenesis and shoot organogenesis from hypocotyl slices of the Collard Greens were established. The combination 20 µM TDZ + 2 µM NAA was optimal for regeneration providing higher total phenolic content.
Jelena Milojevic - One of the best experts on this subject based on the ideXlab platform.
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somatic embryogenesis and shoot organogenesis from the hypocotyl slices and free radical scavenging activity of regenerants of Collard Greens brassica oleracea l var acephala
Plant Cell Tissue and Organ Culture, 2019Co-Authors: Nevena Banjac, Branka Vinterhalter, Dijana Krsticmilosevic, Jelena Milojevic, Ljiljana Tubic, Nabil Ghalawenji, Snežana ZdravkovickoracAbstract:Brassica oleracea var. acephala is an important leafy vegetable that has been widely consumed as a high-nutrient, low-calorie food. Because of the plant’s biennial and self-incompatibility nature, biotechnological approaches are alternative way for propagation and breeding improvements. Since tissue culture studies have been focused in other B. oleracea representatives, the aim of the present study was to achieve effective regeneration protocol distinctive for Collard Greens, and evaluate the total phenolic content and antioxidant activity of regenerants. The effect of 3 cytokinins [thidiazuron (TDZ), 6-benzyladenine (BA) and 6-furfuryladenine (kinetin, KIN)] at increasing concentrations (0, 5, 7.5, 10, 20 or 30 µM) in combination with tenfold lower concentration of 1-naphtaleneacetic acid (NAA) (0, 0.5, 0.75, 1, 2 or 3 µM, respectively) on the regeneration from hypocotyl slices was studied. Histological analysis revealed the two regeneration pathways, somatic embryogenesis and shoot organogenesis, simultaneously occurred in the same explant, regardless of the cytokinin/NAA combinations used. The regeneration frequency of 95.9%, with 7.5 morphogenic structures regenerated per explant, and the healthy appearance of regenerated plants indicated the optimal combination 20 µM TDZ + 2 µM NAA. TDZ at 5 µM provided the high somatic embryo proliferation rate by generation of secondary embryos (7.79) along with the lowest rate of their abnormalities. Embryo-developed plants were successfully acclimatised (above 90%). The plants regenerated and proliferated on TDZ-containing media had higher total phenolic content that correlated with the highest free radical scavenging activity (IC50 = 19.09 µg ml− 1). Somatic embryogenesis and shoot organogenesis from hypocotyl slices of the Collard Greens were established. The combination 20 µM TDZ + 2 µM NAA was optimal for regeneration providing higher total phenolic content.