The Experts below are selected from a list of 360 Experts worldwide ranked by ideXlab platform

Leonardo Di Donna - One of the best experts on this subject based on the ideXlab platform.

  • a comprehensive evaluation of tyrosol and hydroxytyrosol derivatives in extra virgin olive oil by microwave assisted hydrolysis and hplc ms ms
    Analytical and Bioanalytical Chemistry, 2018
    Co-Authors: Lucia Artella, Fabio Mazzotti, Anna Napoli, Giovanni Sindona, Leonardo Di Donna
    Abstract:

    A rapid and reliable method to assay the total amount of tyrosol and hydroxytyrosol derivatives in extra virgin olive oil has been developed. The methodology intends to establish the nutritional quality of this edible oil addressing recent international health claim legislations (the European Commission Regulation No. 432/2012) and changing the classification of extra virgin olive oil to the status of nutraceutical. The method is based on the use of high-performance liquid chromatography coupled with tandem mass spectrometry and labeled internal standards preceded by a fast hydrolysis reaction step performed through the aid of microwaves under acid conditions. The overall process is particularly time saving, much shorter than any methodology previously reported. The developed approach represents a mix of rapidity and accuracy whose values have been found near 100% on different fortified vegetable oils, while the RSD% values, calculated from repeatability and reproducibility experiments, are in all cases under 7%.

Feliciano Priegocapote - One of the best experts on this subject based on the ideXlab platform.

Sarah De Saeger - One of the best experts on this subject based on the ideXlab platform.

  • development and validation of an lc ms ms method for the simultaneous determination of citrinin and ochratoxin a in a variety of feed and foodstuffs
    Journal of Chromatography A, 2018
    Co-Authors: Celine Meerpoel, Siska Croubels, Arnau Vidal, Jose Diana Di Mavungu, Bart Huybrechts, Emmanuel Kossi Tangni, Mathias Devreese, Sarah De Saeger
    Abstract:

    Abstract An ultra-performance liquid chromatography-electrospray tandem mass spectrometry (UPLC-ESI+/−-MS/MS) method for the simultaneous analysis of citrinin (CIT) and ochratoxin A (OTA) in feed (chicken and pig) and food (cereal-based products, fruit, vegetable juices, nuts, seeds, herbs, spices, vegetarian and soy products, alcoholic beverages, baby food products and food supplements) was developed. The mycotoxins were extracted from these matrices using a QuEChERS-based extraction method without any further clean-up step. The samples were 5-fold concentrated. Final extracts were analyzed using a UPLC–MS/MS system and chromatographic separation was achieved by applying a gradient elution for a total run time of 10 min. Mycotoxins were quantified using an internal calibration via analyte/13C-labeled internal standard ratio. The developed method was validated according to the criteria described in Commission Regulation No. 401/2006/EC and Commission Decision No. 2002/657/EC. Specificity, linearity, apparent recovery, limit of detection and quantification, intraday and interday precision, measurement uncertainty, matrix effect, and extraction efficiency were the parameters studied. Finally, 90 Belgian chicken and pig feed samples were analyzed, revealing the simultaneous presence of CIT (

  • hormone and veterinary drug residue analysis in food feed biological and environmental matrices
    Analytical and Bioanalytical Chemistry, 2015
    Co-Authors: Siska Croubels, Peter Van Eenoo, Els Daeseleire, Sarah De Saeger, Lynn Vanhaecke
    Abstract:

    For decades the use of growth-promoting agents and veterinary drugs has been the subject of many international debates, leading to European Council Regulation (EEC) No. 2377/90 introducing maximum residue limits (MRLs) of veterinary medicinal products in food of animal origin. That Regulation was subsequently repealed and replaced by Regulation (EC) No. 470/2009. All the pharmacologically active substances that have been assessed in accordance with this Regulation are listed and classified in Commission Regulation (EU) No. 37/ 2010. Currently, 641 pharmacologically active authorized substances and nine prohibited substances are listed. In addition, food additives with a valid E number which are approved for human consumption are classified as BNo MRL required^. This implies residue surveillance monitoring in food and feed using appropriate analytical methods. These methods may be subdivided into screening methods and confirmatory methods. Similar methods are needed to analyze residues of these compounds in environmental samples and for anti-doping analysis in sports.

  • analysis of eu priority polycyclic aromatic hydrocarbons in food supplements using high performance liquid chromatography coupled to an ultraviolet diode array or fluorescence detector
    Analytica Chimica Acta, 2009
    Co-Authors: Sophie Danyi, Sarah De Saeger, Frana Ois Brose, Catherine Brasseur, Yvesjacques Schneider, Yvan Larondelle, Luc Pussemier, Johan Robbens, Guy Maghuinrogister, Marielouise Scippo
    Abstract:

    High performance liquid chromatography coupled to an ultraviolet, diode array or fluorescence detector (HPLC/UV-FLD) has been used to set up a method to detect the 15(+1) EU priority polycyclic aromatic hydrocarbons (PAHs) in food supplements covering the categories of dried plants and plant extracts excluding oily products. A mini validation was performed and the following parameters have been determined: limit of detection, limit of quantification, precision, recovery and linearity. They were in close agreement with quality criteria described in the Commission Regulation (EC) No 333/2007 concerning the PAH benzo[a]pyrene in foodstuffs, except the not fluorescent cyclopenta[c,d]pyrene for which the UV detection leads to a higher limit of detection. Analysis of twenty commercial food supplements covering mainly the class of dried plants was performed to evaluate their PAHs contamination levels and to test the applicability of the method to various plant matrices. Fifty percent of analyzed samples showed concentration exceeding 2 mu g kg(-1) for one or more PAHs. (C) 2008 Elsevier B.V. All rights reserved.

Aldo Lagana - One of the best experts on this subject based on the ideXlab platform.

  • development and validation of a liquid chromatography atmospheric pressure photoionization tandem mass spectrometric method for the analysis of mycotoxins subjected to Commission Regulation ec no 1881 2006 in cereals
    Journal of Chromatography A, 2010
    Co-Authors: Anna Laura Capriotti, Patrizia Foglia, Riccardo Gubbiotti, Claudia Roccia, Roberto Samperi, Aldo Lagana
    Abstract:

    Abstract A sensitive and reliable liquid chromatography/photoionization (APPI) tandem mass spectrometry method has been developed for determining nine selected mycotoxins in wheat and maize samples. The analytes were chosen on the basis of the mycotoxins under EU Commission Regulation (EC) No. 1881/2006, i.e., deoxynivalenol (DON), zearalenone (ZON), aflatoxins (AFs), and ochratoxin A (OTA), and considering the possibility of a near future Regulation for T-2 and HT-2 toxins. Mycotoxins were extracted from samples by means of an one-step solvent extraction without any cleanup. The developed multi-mycotoxin method permits simultaneous, simple, and rapid determination of several co-existing toxins separated in a single chromatographic run, in which AFs, T-2 and HT-2 toxin are acquired in positive, while OTA, DON and ZON in negative mode. Although a moderate signal suppression was noticeable, matrix effect did not give significant differences at p  = 0.05. Then, calibration in standard solution were used for quantitation. Based on the EU Commission Decision 2002/657/EC, the method was in-house validated in terms of ruggedness, specificity, linearity, trueness, within-laboratory reproducibility, decision limit (CC α ) and detection capability (CC β ). For all the analytes, the regression coefficient r ranged between 0.8752 (DON in wheat) and 0.9465 (ZON in maize), biases related to mean concentrations were from −13% to +12% of the nominal spiking level, and the overall within-laboratory reproducibility ranged 3–16%; finally, CC α values did not differ more than 20% and CC β not more than 42% from their respective maximum limit. Method quantification limits ranged from 1/20 (AFG1) to 1/4 (AFG2 and OTA) the maximum limit established by European Union in the Commission Regulation (EC) No. 1881/2006 and its subsequent amendments.

Lucia Artella - One of the best experts on this subject based on the ideXlab platform.

  • a comprehensive evaluation of tyrosol and hydroxytyrosol derivatives in extra virgin olive oil by microwave assisted hydrolysis and hplc ms ms
    Analytical and Bioanalytical Chemistry, 2018
    Co-Authors: Lucia Artella, Fabio Mazzotti, Anna Napoli, Giovanni Sindona, Leonardo Di Donna
    Abstract:

    A rapid and reliable method to assay the total amount of tyrosol and hydroxytyrosol derivatives in extra virgin olive oil has been developed. The methodology intends to establish the nutritional quality of this edible oil addressing recent international health claim legislations (the European Commission Regulation No. 432/2012) and changing the classification of extra virgin olive oil to the status of nutraceutical. The method is based on the use of high-performance liquid chromatography coupled with tandem mass spectrometry and labeled internal standards preceded by a fast hydrolysis reaction step performed through the aid of microwaves under acid conditions. The overall process is particularly time saving, much shorter than any methodology previously reported. The developed approach represents a mix of rapidity and accuracy whose values have been found near 100% on different fortified vegetable oils, while the RSD% values, calculated from repeatability and reproducibility experiments, are in all cases under 7%.