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Anping Deng - One of the best experts on this subject based on the ideXlab platform.

  • A Competitive Immunoassay for ultrasensitive detection of Hg(2+) in water, human serum and urine samples using immunochromatographic test based on surface-enhanced Raman scattering.
    Analytica chimica acta, 2015
    Co-Authors: Pei She, Hong Wang, Kang Zhao, Yanxin Chu, Chunwei Liu, Xun Guo, Xiang Zhang, Anping Deng
    Abstract:

    An immunochromatographic test (ICT) strip was developed for ultrasensitive Competitive Immunoassay of Hg(2+). This strategy was achieved by combining the easy-operation and rapidity of ICT with the high sensitivity of surface-enhanced Raman scattering (SERS). Monoclonal antibody (mAb) against Hg(2+) and Raman active substance 4-mercaptobenzoic acid (MBA) dual labelled gold nanoparticles (GNPs) were prepared as an immunoprobe. The Raman scattering intensity of MBA on the test line of the ICT strip was measured for quantitative determination of Hg(2+). The ICT was able to directly detect Hg(2+) without complexing due to the specific recognition of the mAb with Hg(2+). The IC50 and limit of detection (LOD) of the assay for Hg(2+) detection were 0.12 ng mL(-1) and 0.45 pg mL(-1), respectively. There was no cross-reactivity (CR) of the assay with other nineteen ions and the ICT strips could be kept for 5 weeks without loss of activity. The recoveries of the assay for water, human serum and urine samples spiked with Hg(2+) were in range of 88.3-107.3% with the relative standard deviations (RSD) of 1.5-9.5% (n = 3). The proposed ICT was used for the detection of Hg(2+) in urine samples collected from Occupational Disease Hospital and the results were confirmed by cold-vapor atomic fluorescence spectroscopy (CV-AFS). The assay exhibited high sensitivity, selectivity, stability, precision and accuracy, demonstrating a promising method for the detection of trace amount of Hg(2+) in environmental water samples and biological serum and urine samples.

  • Flow injection chemiluminescent Competitive Immunoassay for the β-adrenergic agonist salbutamol using carboxylic resin beads and enzymatic amplification
    Sensors and Actuators B-chemical, 2015
    Co-Authors: Mingxia Xu, Anping Deng, Xiaole Qian, Kang Zhao, Jianguo Li
    Abstract:

    Abstract A simple, rapid and sensitive Competitive Immunoassay for the β-adrenergic agonist salbutamol (SAL) was developed with flow injection chemiluminescence (FI-CL) detection. The method was based on immobilizing coating-antigen on carboxylic resin beads and introducing the second antibody tagged with horseradish peroxidase (HRP) into the CL reaction of luminol-PIP-H2O2. The carboxylic resin beads were creatively applied for amplifying signals by carrying more coating-antigen through the chemical bonds between functional groups. With a Competitive format, the salbutamol in solution competed with the coating-antigen for the limited binding sites of the antibody. Then the second antibody labeled with HRP was simply immobilized and an enzyme-enhanced CL emission was obtained due to the catalysis of HRP for the CL reaction. Under the optimal conditions, the CL intensity was linearly related to the concentration of salbutamol in the range of 0.5–100 ng mL−1 with a detection limit (3σ) of 0.15 ng mL−1. This immunosensor exhibited favorable stability, specificity and reproducibility, and was applied to practical samples with satisfactory results. This immunosensing strategy would be of great value in other food additive residues detection.

  • a femtogram level Competitive Immunoassay of mercury ii based on surface enhanced raman spectroscopy
    Chemical Communications, 2014
    Co-Authors: Yuzhen Wang, Shuai Chen, Chao Wei, Jianlin Yao, Anping Deng
    Abstract:

    A femtogram level and specific surface enhanced Raman spectroscopy (SERS) based Competitive Immunoassay was developed to detect Hg(II) in aqueous solution for the first time. This novel approach provides an alternative, ultrasensitive and specific analytical method for the detection of Hg(II).

John C Lieske - One of the best experts on this subject based on the ideXlab platform.

  • Comparison of Urinary Albumin Quantification by Immunoturbidimetry, Competitive Immunoassay, and Protein-Cleavage Liquid Chromatography–Tandem Mass Spectrometry
    Clinical Chemistry, 2009
    Co-Authors: Jesse C. Seegmiller, Timothy M. Borland, Timothy S Larson, Glen L Hortin, Denis Sviridov, John C Lieske
    Abstract:

    Background: Increased urinary albumin excretion is a well-documented diagnostic and prognostic biomarker for renal disease. Urinary albumin is typically measured in clinical settings by Immunoassay methods. However, neither a reference method nor a urine albumin calibration reference material is currently available. Methods: We quantified urinary albumin in patient samples by using 3 commercially available reagent systems: DiaSorin SPQ™ and Beckman Coulter LX® 20 (immunoturbidimetric), and Siemens Immulite® (Competitive Immunoassay). Results were compared to values obtained by protein-cleavage liquid chromatography–tandem mass spectrometry (LC-MS/MS). Results: In general, results from the 3 Immunoassays agreed with results from LC-MS/MS. However, the SPQ results showed a negative bias across all ranges of albuminuria [(0–200 mg/L, y = 0.91 x – 3.74 (CI 0.86–0.96); > 200 mg/L, y = 0.88 x – 40.30 (CI 0.76–1.00)], whereas the LX 20 showed minimal bias in the 0–200 mg/L range [ y = 0.97 x − 88 (CI 0.92–1.02)] and the Immulite assay showed positive bias in the 0–200 mg/L range [ y = 1.15 x – 4.38 (CI 1.09–1.20)]. Conclusions: These results showed a reasonable quantification of urinary albumin by representative polyclonal and monoclonal Immunoassays compared to an LC-MS/MS assay. In addition, the results do not suggest the presence of nonimmunoreactive albumin in urine. However, differences in analytic performance between assays support the need for a reference calibration material and reference method to standardize clinical laboratory measurements of urinary albumin.

  • comparison of urinary albumin quantification by immunoturbidimetry Competitive Immunoassay and protein cleavage liquid chromatography tandem mass spectrometry
    Clinical Chemistry, 2009
    Co-Authors: Jesse C. Seegmiller, Timothy M. Borland, Timothy S Larson, Glen L Hortin, Denis Sviridov, John C Lieske
    Abstract:

    Background: Increased urinary albumin excretion is a well-documented diagnostic and prognostic biomarker for renal disease. Urinary albumin is typically measured in clinical settings by Immunoassay methods. However, neither a reference method nor a urine albumin calibration reference material is currently available. Methods: We quantified urinary albumin in patient samples by using 3 commercially available reagent systems: DiaSorin SPQ™ and Beckman Coulter LX® 20 (immunoturbidimetric), and Siemens Immulite® (Competitive Immunoassay). Results were compared to values obtained by protein-cleavage liquid chromatography–tandem mass spectrometry (LC-MS/MS). Results: In general, results from the 3 Immunoassays agreed with results from LC-MS/MS. However, the SPQ results showed a negative bias across all ranges of albuminuria [(0–200 mg/L, y = 0.91 x – 3.74 (CI 0.86–0.96); > 200 mg/L, y = 0.88 x – 40.30 (CI 0.76–1.00)], whereas the LX 20 showed minimal bias in the 0–200 mg/L range [ y = 0.97 x − 88 (CI 0.92–1.02)] and the Immulite assay showed positive bias in the 0–200 mg/L range [ y = 1.15 x – 4.38 (CI 1.09–1.20)]. Conclusions: These results showed a reasonable quantification of urinary albumin by representative polyclonal and monoclonal Immunoassays compared to an LC-MS/MS assay. In addition, the results do not suggest the presence of nonimmunoreactive albumin in urine. However, differences in analytic performance between assays support the need for a reference calibration material and reference method to standardize clinical laboratory measurements of urinary albumin.

Yuzhen Wang - One of the best experts on this subject based on the ideXlab platform.

Jan Van Den Abbeele - One of the best experts on this subject based on the ideXlab platform.

  • Description of a nanobody-based Competitive Immunoassay to detect tsetse fly exposure.
    PLoS neglected tropical diseases, 2015
    Co-Authors: Guy Caljon, Shahid Hussain, Lieve Vermeiren, Jan Van Den Abbeele
    Abstract:

    Tsetse flies are the main vectors of human and animal African trypanosomes. The Tsal proteins in tsetse fly saliva were previously identified as suitable biomarkers of bite exposure. A new Competitive assay was conceived based on nanobody (Nb) technology to ameliorate the detection of anti-Tsal antibodies in mammalian hosts. A camelid-derived Nb library was generated against the Glossina morsitans morsitans sialome and exploited to select Tsal specific Nbs. One of the three identified Nb families (family III, TsalNb-05 and TsalNb-11) was found suitable for anti-Tsal antibody detection in a Competitive ELISA format. The Competitive ELISA was able to detect exposure to a broad range of tsetse species (G. morsitans morsitans, G. pallidipes, G. palpalis gambiensis and G. fuscipes) and did not cross-react with the other hematophagous insects (Stomoxys calcitrans and Tabanus yao). Using a collection of plasmas from tsetse-exposed pigs, the new test characteristics were compared with those of the previously described G. m. moristans and rTsal1 indirect ELISAs, revealing equally good specificities (> 95%) and positive predictive values (> 98%) but higher negative predictive values and hence increased sensitivity (> 95%) and accuracy (> 95%). We have developed a highly accurate Nb-based Competitive Immunoassay to detect specific anti-Tsal antibodies induced by various tsetse fly species in a range of hosts. We propose that this Competitive assay provides a simple serological indicator of tsetse fly presence without the requirement of test adaptation to the vertebrate host species. In addition, the use of monoclonal Nbs for antibody detection is innovative and could be applied to other tsetse fly salivary biomarkers in order to achieve a multi-target immunoprofiling of hosts. In addition, this approach could be broadened to other pathogenic organisms for which accurate serological diagnosis remains a bottleneck.

  • description of a nanobody based Competitive Immunoassay to detect tsetse fly exposure
    PLOS Neglected Tropical Diseases, 2015
    Co-Authors: Guy Caljon, Shahid Hussain, Lieve Vermeiren, Jan Van Den Abbeele
    Abstract:

    Background Tsetse flies are the main vectors of human and animal African trypanosomes. The Tsal proteins in tsetse fly saliva were previously identified as suitable biomarkers of bite exposure. A new Competitive assay was conceived based on nanobody (Nb) technology to ameliorate the detection of anti-Tsal antibodies in mammalian hosts.

Jianlin Yao - One of the best experts on this subject based on the ideXlab platform.

  • Magnetic separation of clenbuterol based on Competitive Immunoassay and evaluation by surface-enhanced Raman spectroscopy
    RSC Advances, 2017
    Co-Authors: Chao Wei, Yaxian Yuan, Yi Zong, Qinghua Guo, Jianlin Yao
    Abstract:

    The elimination of β-agonist has attracted considerable interest due to its harmfulness to human health when it existed in pork. Here, a strategy based on immuno-magnetic nanoparticles has been successfully developed for the selective and successive magnetic separation of two kinds of β-agonists, clenbuterol (CL) and salbutamol (SAL). The calibration curve of Competitive Immunoassay was determined for the estimation of the final concentration of targets after the separation, in which the limit of detection (LOD) and half maximal inhibitory concentration (IC50) were about 17 fg mL−1 and 193 pg mL−1, respectively. The specific interaction between the target and the complementary antibody attached to Fe3O4@Au nanoparticles resulted in the aggregation of Fe3O4@Au nanoparticles carried with targets. The magnetic collection of the aggregation of Fe3O4@Au nanoparticles decreased the concentration of targets significantly. The results revealed that the final concentration of remaining targets was lower than the LOD. This strategy was employed to separate CL and SAL molecules in mixed solutions simultaneously or successively with high efficiency. The results demonstrate that it provides a selective and effective approach for the removal of harmful residues in practical samples.

  • a femtogram level Competitive Immunoassay of mercury ii based on surface enhanced raman spectroscopy
    Chemical Communications, 2014
    Co-Authors: Yuzhen Wang, Shuai Chen, Chao Wei, Jianlin Yao, Anping Deng
    Abstract:

    A femtogram level and specific surface enhanced Raman spectroscopy (SERS) based Competitive Immunoassay was developed to detect Hg(II) in aqueous solution for the first time. This novel approach provides an alternative, ultrasensitive and specific analytical method for the detection of Hg(II).