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Rosanna Adone - One of the best experts on this subject based on the ideXlab platform.

  • development of a sterne based Complement Fixation Test to monitor the humoral response induced by anthrax vaccines
    Frontiers in Microbiology, 2016
    Co-Authors: Rosanna Adone, Massimiliano Francia, Michela Sali, Michela Iatarola, Adelia Donatiello, Antonio Fasanella
    Abstract:

    Anthrax is a zoonotic disease caused by Bacillus anthracis spore-forming bacterium. Since it is primarily a disease of animals, the control in animals and humans depends on the prevention in livestock, principally cattle, sheep and goats. Most veterinary vaccines utilize the toxigenic, uncapsulated (pXO1+/pXO2–) B.anthracis strain 34F2 which affords protection through the production of neutralizing antibodies directed to the toxin components Protective Antigen (PA), Lethal Factor (LF) and Edema Factor (EF). The titration of specific antibodies in sera of vaccinated animals is crucial to evaluate the efficacy of the vaccination and to obtain epidemiological information for an effective anthrax surveillance. In this study, we developed a Sterne-based Complement Fixation Test to detect specific antibodies induced in animals vaccinated with Sterne 34F2 . We assessed its efficacy in laboratory animals and under field conditions by monitoring the humoral response induced by vaccination in cattle. The results indicated that the Sterne-based CFT is able to identify vaccinated animals with a good sensitivity and specificity offering many benefits especially with regard to costs, standardization and reproducibility of the assay procedure.

  • Brucella melitensis B115-based Complement Fixation Test to detect antibodies induced by Brucella rough strains.
    Journal of Applied Microbiology, 2008
    Co-Authors: Rosanna Adone, Massimiliano Francia, Franco Ciuchini
    Abstract:

    Aims:  To assess the efficiency of a Brucella melitensis B115 rough strain, naturally devoid of antiComplementary activity, used as antigen in a Complement Fixation Test (CFT) to detect antibodies induced by Brucella strains with rough phenotype, such as Brucella abortus RB51, Brucella ovis and Brucella canis. Methods and Results:  Complement Fixation Testing was performed on sera from RB51-vaccinated cattle and buffaloes, B. ovis-infected sheep and B. canis-infected dogs using B115, RB51 and the hot saline extract (HSE) as antigens. The B115-based CFT proved highly sensitive and specific in detecting rough antibodies and its efficiency was comparable with that of RB51 and HSE-based CFT. Conclusions: Brucella melitensis B115 can be successfully used as an antigen in CFT to detect antibodies induced by Brucella rough strains. Significance and Impact of the Study: Brucella melitensis B115 antigen may represent an improvement over Brucella rough strains for Brucella antibody detection by CFT, thus enhancing the efficiency of brucellosis surveillance systems. Owing to the absence of antiComplementary activity, it does not require particular growth conditions or modifications and can be accurately standardized. The B115-based CFT may constitute a suitable supplementary Test for the diagnosis of human infections owing to rough Brucellae.

  • Brucella melitensis B115-based Complement Fixation Test to detect antibodies induced by Brucella rough strains.
    Journal of Applied Microbiology, 2008
    Co-Authors: Rosanna Adone, Massimiliano Francia, Franco Ciuchini
    Abstract:

    Aims:  To assess the efficiency of a Brucella melitensis B115 rough strain, naturally devoid of antiComplementary activity, used as antigen in a Complement Fixation Test (CFT) to detect antibodies induced by Brucella strains with rough phenotype, such as Brucella abortus RB51, Brucella ovis and Brucella canis. Methods and Results:  Complement Fixation Testing was performed on sera from RB51-vaccinated cattle and buffaloes, B. ovis-infected sheep and B. canis-infected dogs using B115, RB51 and the hot saline extract (HSE) as antigens. The B115-based CFT proved highly sensitive and specific in detecting rough antibodies and its efficiency was comparable with that of RB51 and HSE-based CFT. Conclusions: Brucella melitensis B115 can be successfully used as an antigen in CFT to detect antibodies induced by Brucella rough strains. Significance and Impact of the Study: Brucella melitensis B115 antigen may represent an improvement over Brucella rough strains for Brucella antibody detection by CFT, thus enhancing the efficiency of brucellosis surveillance systems. Owing to the absence of antiComplementary activity, it does not require particular growth conditions or modifications and can be accurately standardized. The B115-based CFT may constitute a suitable supplementary Test for the diagnosis of human infections owing to rough Brucellae.

Franco Ciuchini - One of the best experts on this subject based on the ideXlab platform.

  • Brucella melitensis B115-based Complement Fixation Test to detect antibodies induced by Brucella rough strains.
    Journal of Applied Microbiology, 2008
    Co-Authors: Rosanna Adone, Massimiliano Francia, Franco Ciuchini
    Abstract:

    Aims:  To assess the efficiency of a Brucella melitensis B115 rough strain, naturally devoid of antiComplementary activity, used as antigen in a Complement Fixation Test (CFT) to detect antibodies induced by Brucella strains with rough phenotype, such as Brucella abortus RB51, Brucella ovis and Brucella canis. Methods and Results:  Complement Fixation Testing was performed on sera from RB51-vaccinated cattle and buffaloes, B. ovis-infected sheep and B. canis-infected dogs using B115, RB51 and the hot saline extract (HSE) as antigens. The B115-based CFT proved highly sensitive and specific in detecting rough antibodies and its efficiency was comparable with that of RB51 and HSE-based CFT. Conclusions: Brucella melitensis B115 can be successfully used as an antigen in CFT to detect antibodies induced by Brucella rough strains. Significance and Impact of the Study: Brucella melitensis B115 antigen may represent an improvement over Brucella rough strains for Brucella antibody detection by CFT, thus enhancing the efficiency of brucellosis surveillance systems. Owing to the absence of antiComplementary activity, it does not require particular growth conditions or modifications and can be accurately standardized. The B115-based CFT may constitute a suitable supplementary Test for the diagnosis of human infections owing to rough Brucellae.

  • Brucella melitensis B115-based Complement Fixation Test to detect antibodies induced by Brucella rough strains.
    Journal of Applied Microbiology, 2008
    Co-Authors: Rosanna Adone, Massimiliano Francia, Franco Ciuchini
    Abstract:

    Aims:  To assess the efficiency of a Brucella melitensis B115 rough strain, naturally devoid of antiComplementary activity, used as antigen in a Complement Fixation Test (CFT) to detect antibodies induced by Brucella strains with rough phenotype, such as Brucella abortus RB51, Brucella ovis and Brucella canis. Methods and Results:  Complement Fixation Testing was performed on sera from RB51-vaccinated cattle and buffaloes, B. ovis-infected sheep and B. canis-infected dogs using B115, RB51 and the hot saline extract (HSE) as antigens. The B115-based CFT proved highly sensitive and specific in detecting rough antibodies and its efficiency was comparable with that of RB51 and HSE-based CFT. Conclusions: Brucella melitensis B115 can be successfully used as an antigen in CFT to detect antibodies induced by Brucella rough strains. Significance and Impact of the Study: Brucella melitensis B115 antigen may represent an improvement over Brucella rough strains for Brucella antibody detection by CFT, thus enhancing the efficiency of brucellosis surveillance systems. Owing to the absence of antiComplementary activity, it does not require particular growth conditions or modifications and can be accurately standardized. The B115-based CFT may constitute a suitable supplementary Test for the diagnosis of human infections owing to rough Brucellae.

Massimiliano Francia - One of the best experts on this subject based on the ideXlab platform.

  • development of a sterne based Complement Fixation Test to monitor the humoral response induced by anthrax vaccines
    Frontiers in Microbiology, 2016
    Co-Authors: Rosanna Adone, Massimiliano Francia, Michela Sali, Michela Iatarola, Adelia Donatiello, Antonio Fasanella
    Abstract:

    Anthrax is a zoonotic disease caused by Bacillus anthracis spore-forming bacterium. Since it is primarily a disease of animals, the control in animals and humans depends on the prevention in livestock, principally cattle, sheep and goats. Most veterinary vaccines utilize the toxigenic, uncapsulated (pXO1+/pXO2–) B.anthracis strain 34F2 which affords protection through the production of neutralizing antibodies directed to the toxin components Protective Antigen (PA), Lethal Factor (LF) and Edema Factor (EF). The titration of specific antibodies in sera of vaccinated animals is crucial to evaluate the efficacy of the vaccination and to obtain epidemiological information for an effective anthrax surveillance. In this study, we developed a Sterne-based Complement Fixation Test to detect specific antibodies induced in animals vaccinated with Sterne 34F2 . We assessed its efficacy in laboratory animals and under field conditions by monitoring the humoral response induced by vaccination in cattle. The results indicated that the Sterne-based CFT is able to identify vaccinated animals with a good sensitivity and specificity offering many benefits especially with regard to costs, standardization and reproducibility of the assay procedure.

  • Brucella melitensis B115-based Complement Fixation Test to detect antibodies induced by Brucella rough strains.
    Journal of Applied Microbiology, 2008
    Co-Authors: Rosanna Adone, Massimiliano Francia, Franco Ciuchini
    Abstract:

    Aims:  To assess the efficiency of a Brucella melitensis B115 rough strain, naturally devoid of antiComplementary activity, used as antigen in a Complement Fixation Test (CFT) to detect antibodies induced by Brucella strains with rough phenotype, such as Brucella abortus RB51, Brucella ovis and Brucella canis. Methods and Results:  Complement Fixation Testing was performed on sera from RB51-vaccinated cattle and buffaloes, B. ovis-infected sheep and B. canis-infected dogs using B115, RB51 and the hot saline extract (HSE) as antigens. The B115-based CFT proved highly sensitive and specific in detecting rough antibodies and its efficiency was comparable with that of RB51 and HSE-based CFT. Conclusions: Brucella melitensis B115 can be successfully used as an antigen in CFT to detect antibodies induced by Brucella rough strains. Significance and Impact of the Study: Brucella melitensis B115 antigen may represent an improvement over Brucella rough strains for Brucella antibody detection by CFT, thus enhancing the efficiency of brucellosis surveillance systems. Owing to the absence of antiComplementary activity, it does not require particular growth conditions or modifications and can be accurately standardized. The B115-based CFT may constitute a suitable supplementary Test for the diagnosis of human infections owing to rough Brucellae.

  • Brucella melitensis B115-based Complement Fixation Test to detect antibodies induced by Brucella rough strains.
    Journal of Applied Microbiology, 2008
    Co-Authors: Rosanna Adone, Massimiliano Francia, Franco Ciuchini
    Abstract:

    Aims:  To assess the efficiency of a Brucella melitensis B115 rough strain, naturally devoid of antiComplementary activity, used as antigen in a Complement Fixation Test (CFT) to detect antibodies induced by Brucella strains with rough phenotype, such as Brucella abortus RB51, Brucella ovis and Brucella canis. Methods and Results:  Complement Fixation Testing was performed on sera from RB51-vaccinated cattle and buffaloes, B. ovis-infected sheep and B. canis-infected dogs using B115, RB51 and the hot saline extract (HSE) as antigens. The B115-based CFT proved highly sensitive and specific in detecting rough antibodies and its efficiency was comparable with that of RB51 and HSE-based CFT. Conclusions: Brucella melitensis B115 can be successfully used as an antigen in CFT to detect antibodies induced by Brucella rough strains. Significance and Impact of the Study: Brucella melitensis B115 antigen may represent an improvement over Brucella rough strains for Brucella antibody detection by CFT, thus enhancing the efficiency of brucellosis surveillance systems. Owing to the absence of antiComplementary activity, it does not require particular growth conditions or modifications and can be accurately standardized. The B115-based CFT may constitute a suitable supplementary Test for the diagnosis of human infections owing to rough Brucellae.

Antonio Fasanella - One of the best experts on this subject based on the ideXlab platform.

  • development of a sterne based Complement Fixation Test to monitor the humoral response induced by anthrax vaccines
    Frontiers in Microbiology, 2016
    Co-Authors: Rosanna Adone, Massimiliano Francia, Michela Sali, Michela Iatarola, Adelia Donatiello, Antonio Fasanella
    Abstract:

    Anthrax is a zoonotic disease caused by Bacillus anthracis spore-forming bacterium. Since it is primarily a disease of animals, the control in animals and humans depends on the prevention in livestock, principally cattle, sheep and goats. Most veterinary vaccines utilize the toxigenic, uncapsulated (pXO1+/pXO2–) B.anthracis strain 34F2 which affords protection through the production of neutralizing antibodies directed to the toxin components Protective Antigen (PA), Lethal Factor (LF) and Edema Factor (EF). The titration of specific antibodies in sera of vaccinated animals is crucial to evaluate the efficacy of the vaccination and to obtain epidemiological information for an effective anthrax surveillance. In this study, we developed a Sterne-based Complement Fixation Test to detect specific antibodies induced in animals vaccinated with Sterne 34F2 . We assessed its efficacy in laboratory animals and under field conditions by monitoring the humoral response induced by vaccination in cattle. The results indicated that the Sterne-based CFT is able to identify vaccinated animals with a good sensitivity and specificity offering many benefits especially with regard to costs, standardization and reproducibility of the assay procedure.

Miklos Sardy - One of the best experts on this subject based on the ideXlab platform.

  • Complement Fixation Test an update of an old method for diagnosis of bullous pemphigoid
    Acta Dermato-venereologica, 2016
    Co-Authors: Jana Jankaskova, Orsolya N Horvath, Rita Varga, Thomas Ruzicka, Miklos Sardy
    Abstract:

    The Complement Fixation Test (CFT) is a method traditionally used for diagnosis of gestational pemphigoid. Its performance in diagnosis of bullous pemphigoid (BP) has not been investigated in a large patient cohort. The aim of this single-centre, retrospective, serological case-control study of 300 patients with BP and 136 control patients was to analyse its operating characteristics. CFT was found to have a sensitivity of 71.7% and a specificity of 100%. Furthermore, CFT diagnosed 20 of 46 patients with BP (43.5%) who were negative for both BP180 and BP230 enzyme-linked immunoassays (ELISAs), 31 of 66 patients (47.0%) who were negative for indirect immunofluorescence of the oesophagus, 5 of 14 patients (35.7%) who were serologically negative for all investigated serological assays, and 7 of 18 patients (38.9%) in whom direct immunofluorescence was negative. Combination of CFT with all other serological assays resulted in a sensitivity of 95.3%. In conclusion, CFT is suitable for the diagnosis of BP, and can help to diagnose serologically challenging cases.