The Experts below are selected from a list of 183 Experts worldwide ranked by ideXlab platform
Robert C. Thompson - One of the best experts on this subject based on the ideXlab platform.
-
primary structure and functional expression from Complementary DNA of a human interleukin 1 receptor antagonist
Nature, 1990Co-Authors: Stephen P. Eisenberg, Ronald Evans, E Verderber, Michael T Brewer, Charles H Hannum, William P Arend, Robert C. ThompsonAbstract:Human monocytes induced with adherent IgG secrete an interleukin-1 receptor antagonist which could be important for the in vivo regulation of IL-1 activity. A Complementary DNA for this molecule has been isolated from a human monocyte library. Analysis of monocyte RNA indicates that the gene is transcriptionally regulated. The sequence of the receptor antagonist indicates that it is structurally similar to IL-lp. Expression of the cDNA in Escherichia coli yields IL-1 receptor antagonist activity.
-
Primary structure and functional expression from Complementary DNA of a human interleukin-1 receptor antagonist
Nature, 1990Co-Authors: Stephen P. Eisenberg, Ronald Evans, E Verderber, Michael T Brewer, Charles H Hannum, William P Arend, Robert C. ThompsonAbstract:Human monocytes induced with adherent IgG secrete an interleukin-1 receptor antagonist which could be important for the in vivo regulation of IL-1 activity. A Complementary DNA for this molecule has been isolated from a human monocyte library. Analysis of monocyte RNA indicates that the gene is transcriptionally regulated. The sequence of the receptor antagonist indicates that it is structurally similar to IL-1 beta. Expression of the cDNA in Escherichia coli yields IL-1 receptor antagonist activity.
Alexey Soldatov - One of the best experts on this subject based on the ideXlab platform.
-
Transcriptome analysis by strand-specific sequencing of Complementary DNA
Nucleic Acids Research, 2009Co-Authors: Dmitri Parkhomchuk, Tatiana Borodina, Vyacheslav Amstislavskiy, Maria Banaru, Linda Hallen, Sylvia Krobitsch, Hans Lehrach, Alexey SoldatovAbstract:High-throughput Complementary DNA sequencing (RNA-Seq) is a powerful tool for whole-transcriptome analysis, supplying information about a transcript's expression level and structure. However, it is difficult to determine the polarity of transcripts, and therefore identify which strand is transcribed. Here, we present a simple cDNA sequencing protocol that preserves information about a transcript's direction. Using Saccharomyces cerevisiae and mouse brain transcriptomes as models, we demonstrate that knowing the transcript's orientation allows more accurate determination of the structure and expression of genes. It also helps to identify new genes and enables studying promoter-associated and antisense transcription. The transcriptional landscapes we obtained are available online.
Stephen P. Eisenberg - One of the best experts on this subject based on the ideXlab platform.
-
primary structure and functional expression from Complementary DNA of a human interleukin 1 receptor antagonist
Nature, 1990Co-Authors: Stephen P. Eisenberg, Ronald Evans, E Verderber, Michael T Brewer, Charles H Hannum, William P Arend, Robert C. ThompsonAbstract:Human monocytes induced with adherent IgG secrete an interleukin-1 receptor antagonist which could be important for the in vivo regulation of IL-1 activity. A Complementary DNA for this molecule has been isolated from a human monocyte library. Analysis of monocyte RNA indicates that the gene is transcriptionally regulated. The sequence of the receptor antagonist indicates that it is structurally similar to IL-lp. Expression of the cDNA in Escherichia coli yields IL-1 receptor antagonist activity.
-
Primary structure and functional expression from Complementary DNA of a human interleukin-1 receptor antagonist
Nature, 1990Co-Authors: Stephen P. Eisenberg, Ronald Evans, E Verderber, Michael T Brewer, Charles H Hannum, William P Arend, Robert C. ThompsonAbstract:Human monocytes induced with adherent IgG secrete an interleukin-1 receptor antagonist which could be important for the in vivo regulation of IL-1 activity. A Complementary DNA for this molecule has been isolated from a human monocyte library. Analysis of monocyte RNA indicates that the gene is transcriptionally regulated. The sequence of the receptor antagonist indicates that it is structurally similar to IL-1 beta. Expression of the cDNA in Escherichia coli yields IL-1 receptor antagonist activity.
Dmitri Parkhomchuk - One of the best experts on this subject based on the ideXlab platform.
-
Transcriptome analysis by strand-specific sequencing of Complementary DNA
Nucleic Acids Research, 2009Co-Authors: Dmitri Parkhomchuk, Tatiana Borodina, Vyacheslav Amstislavskiy, Maria Banaru, Linda Hallen, Sylvia Krobitsch, Hans Lehrach, Alexey SoldatovAbstract:High-throughput Complementary DNA sequencing (RNA-Seq) is a powerful tool for whole-transcriptome analysis, supplying information about a transcript's expression level and structure. However, it is difficult to determine the polarity of transcripts, and therefore identify which strand is transcribed. Here, we present a simple cDNA sequencing protocol that preserves information about a transcript's direction. Using Saccharomyces cerevisiae and mouse brain transcriptomes as models, we demonstrate that knowing the transcript's orientation allows more accurate determination of the structure and expression of genes. It also helps to identify new genes and enables studying promoter-associated and antisense transcription. The transcriptional landscapes we obtained are available online.
Katsuhiko Mikoshiba - One of the best experts on this subject based on the ideXlab platform.
-
primary structure and functional expression from Complementary DNA of a brain calcium channel
Nature, 1991Co-Authors: Yasuo Mori, Atsushi Mikami, Thomas Friedrich, Junichi Nakai, Peter Ruth, Eva Bosse, Franz Hofmann, Veit Flockerzi, Teiichi Furuichi, Katsuhiko MikoshibaAbstract:The primary structure of a voltage-dependent cal-cium channel from rabbit brain has been deduced by cloning and sequencing the Complementary DNA. Calcium channel activity expressed from the cDNA is dramatically increased by coexpression of the α2 and β subunits, known to be associated with the dihydropyridine receptor. This channel is a high voltage-activated calcium channel that is insensitive both to nifedipine and to ω-conotoxin. We suggest that it is expressed predominantly in cerebellar Purkinje cells and granule cells.