The Experts below are selected from a list of 200295 Experts worldwide ranked by ideXlab platform
Bart Landuyt - One of the best experts on this subject based on the ideXlab platform.
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differential peptidomics highlights adipokinetic hormone as key player in regulating digestion in anautogenous flesh fly sarcophaga crassipalpis
General and Comparative Endocrinology, 2014Co-Authors: Valerie Broeckx, Bart LanduytAbstract:Abstract Females of anautogenous flesh flies, Sarcophaga crassipalpis, need a Protein meal in order to produce their first batch of eggs. This Protein meal elicits an increase in midgut proteolytic activity that is under neuropeptidergic regulation. Time series of decapitation and rescue experiments of liver fed flies evidenced the need of a peptide factor released by corpora cardiaca (CC) within 4 h post Protein feeding in order to assure Complete Protein digestion. Q-Exactive quantitative differential peptidomics analysis on CC of sugar fed flies and flies 5 h post Protein feeding respectively, showed a unique consistent decrease in the stored amount of adipokinetic hormone (AKH) ranging between 16% up to 63%. Injection of AKH into liver fed decapitated flies as well as sugar fed intact flies resulted in dose dependent enhanced midgut proteolytic activity up to the level of intact Protein fed flies. This suggests a key role of AKH in food depended reproduction.
Shamil R Sunyaev - One of the best experts on this subject based on the ideXlab platform.
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increase of functional diversity by alternative splicing
Trends in Genetics, 2003Co-Authors: Evgenia V Kriventseva, Ina Koch, Rolf Apweiler, Martin Vingron, Peer Bork, Mikhail S Gelfand, Shamil R SunyaevAbstract:A large-scale analysis of Protein isoforms arising from alternative splicing shows that alternative splicing tends to insert or delete Complete Protein domains more frequently than expected by chance, whereas disruption of domains and other structural modules is less frequent. If domain regions are disrupted, the functional effect, as predicted from 3D structure, is frequently equivalent to removal of the entire domain. Also, short alternative splicing events within domains, which might preserve folded structure, target functional residues more frequently than expected. Thus, it seems that positive selection has had a major role in the evolution of alternative splicing.
Valerie Broeckx - One of the best experts on this subject based on the ideXlab platform.
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differential peptidomics highlights adipokinetic hormone as key player in regulating digestion in anautogenous flesh fly sarcophaga crassipalpis
General and Comparative Endocrinology, 2014Co-Authors: Valerie Broeckx, Bart LanduytAbstract:Abstract Females of anautogenous flesh flies, Sarcophaga crassipalpis, need a Protein meal in order to produce their first batch of eggs. This Protein meal elicits an increase in midgut proteolytic activity that is under neuropeptidergic regulation. Time series of decapitation and rescue experiments of liver fed flies evidenced the need of a peptide factor released by corpora cardiaca (CC) within 4 h post Protein feeding in order to assure Complete Protein digestion. Q-Exactive quantitative differential peptidomics analysis on CC of sugar fed flies and flies 5 h post Protein feeding respectively, showed a unique consistent decrease in the stored amount of adipokinetic hormone (AKH) ranging between 16% up to 63%. Injection of AKH into liver fed decapitated flies as well as sugar fed intact flies resulted in dose dependent enhanced midgut proteolytic activity up to the level of intact Protein fed flies. This suggests a key role of AKH in food depended reproduction.
Eckard Wimmer - One of the best experts on this subject based on the ideXlab platform.
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Complete Protein linkage map between the p2 and p3 non structural Proteins of poliovirus
Journal of General Virology, 2007Co-Authors: Jiang Yin, Eckard Wimmer, Ying Liu, Aniko V PaulAbstract:All of the non-structural Proteins of poliovirus, including their processing precursors, are involved in the replication of the viral RNA genome. These Proteins assemble into a replication complex, which also contains the viral RNA and cellular factors. An understanding of how these viral Proteins interact with each other would enhance our understanding of the molecular events occurring during poliovirus infection of the cell. Previously, we have employed the yeast two-hybrid system to construct two separate linkage maps for the polioviral P2 and P3 Proteins, respectively. In the present study, we have searched for interacting pairs between the P2 and P3 Proteins in a similar inducible yeast two-hybrid system. Although, the primary functions of the proteolytic products of the P2 and P3 domains of the polyProtein in the viral life cycle are different, we observed significant interactions between 2CATPase and 3AB; 2Apro and 3A, 3Cpro or 3Dpol; 2B and 3A or 3AB. All of the interactions were measured in the yeast two-hybrid system by exchanging the interacting pairs on the transcription-activation and DNA-binding constructs. In vitro GST pull-down assay suggested that the 2CATPase/3AB interaction involves both ionic and hydrophobic contacts between the two Proteins. The possible biological implication of the interactions observed in the yeast two-hybrid system will be discussed.
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Complete Protein linkage map of poliovirus p3 Proteins interaction of polymerase 3dpol with vpg and with genetic variants of 3ab
Journal of Virology, 1998Co-Authors: Wenkai Xiang, Andrea Cuconati, Debra A Hope, Karla Kirkegaard, Eckard WimmerAbstract:Poliovirus has evolved to maximize its genomic information by producing multifunctional viral Proteins. The P3 nonstructural Proteins harbor various activities when paired with different binding partners. These viral polypeptides regulate host cell macromolecular synthesis and function as Proteinases, as RNA binding Proteins, or as RNA-dependent RNA polymerase. A cleavage product of the P3 region is the genome-linked Protein VPg that is essential in the initiation of RNA synthesis. We have used an inducible yeast two-hybrid system to analyze directly Protein-Protein interactions among P3 Proteins. Sixteen signals of homo- or heterodimer interactions have been observed and have been divided into three groups. Of interest is the newly discovered affinity of VPg to 3Dpol that suggests direct interaction between these molecules in genome replication. A battery of 3AB variants (eight clustered-charge-to-alanine changes and five single-amino-acid mutations) has been used to map the binding determinants of 3AB-3AB interaction which were found to differ from the amino acids critical for the 3AB-3Dpol interaction. The viral Proteinase 3Cpro was not found to interact with other 3Cpro molecules or with any other P3 polypeptide in yeast cells, a result confirmed by glutaraldehyde cross-linking. The weak apparent interaction between 3AB and 3CDpro scored in the yeast two-hybrid system was in contrast to a strong signal by far-Western blotting. The results elucidate, in part, previous results of biochemical and genetic analyses. The role of the interactions in RNA replication is addressed.
Thomas Szyperski - One of the best experts on this subject based on the ideXlab platform.
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g matrix fourier transform nmr spectroscopy for Complete Protein resonance assignment
Proceedings of the National Academy of Sciences of the United States of America, 2004Co-Authors: Hanudatta S Atreya, Thomas SzyperskiAbstract:A G-matrix Fourier transform (GFT) NMR spectroscopy-based strategy for resonance assignment of Proteins is described. Each of the GFT NMR experiments presented here rapidly affords four-, five-, or six-dimensional spectral information in combination with precise measurements of chemical shifts. The resulting high information content enables one to obtain nearly Complete assignments by using only four NMR experiments. For the backbone amide proton detected “out-and-back” experiments, data collection was further accelerated up to ≈2.5-fold by use of longitudinal 1H relaxation optimization. The GFT NMR experiments were acquired for three Proteins with molecular masses ranging from 8.6 to 17 kDa, demonstrating that the proposed strategy is of key interest for automated resonance assignment in structural genomics.
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reduced dimensionality nmr spectroscopy for high throughput Protein resonance assignment
Proceedings of the National Academy of Sciences of the United States of America, 2002Co-Authors: Thomas Szyperski, Deok C Yeh, Dinesh K Sukumaran, Hunter N B Moseley, Gaetano T MontelioneAbstract:A suite of reduced-dimensionality 13C,15N,1H-triple-resonance NMR experiments is presented for rapid and Complete Protein resonance assignment. Even when using short measurement times, these experiments allow one to retain the high spectral resolution required for efficient automated analysis. “Sampling limited” and “sensitivity limited” data collection regimes are defined, respectively, depending on whether the sampling of the indirect dimensions or the sensitivity of a multidimensional NMR experiments per se determines the minimally required measurement time. We show that reduced-dimensionality NMR spectroscopy is a powerful approach to avoid the “sampling limited regime”—i.e., a standard set of ten experiments proposed here allows one to effectively adapt minimal measurement times to sensitivity requirements. This is of particular interest in view of the greatly increased sensitivity of NMR spectrometers equipped with cryogenic probes. As a step toward fully automated analysis, the program autoassign has been extended to provide sequential backbone and 13Cβ resonance assignments from these reduced-dimensionality NMR data.