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Thomas Pape - One of the best experts on this subject based on the ideXlab platform.
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a new species of Sarcophaga meigen subgenus hoa rohdendorf diptera sarcophagidae
Zootaxa, 2020Co-Authors: Chao Wang, Dong Zhang, Wanqi Xue, Thomas PapeAbstract:Sarcophaga (Hoa) membranijuxta sp. nov. is described from the Chinese provinces Guangxi and Hainan and documented with illustrations, photographs and scanning electron microscopy images. The habitus and male terminalia of Sarcophaga (Hoa) basiseta Baranov, 1931 are documented with photographs for the first time, and the circumscription and distribution of the subgenus Hoa Rohdendorf, 1937 are updated.
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Checklist of the Sarcophagidae (Diptera) of Croatia, with new records from Croatia and other Mediterranean countries
'Pensoft Publishers', 2019Co-Authors: Stjepan Krčmar, Thomas Pape, Daniel Whitmore, Eliana BuenaventuraAbstract:An updated checklist of Croatian flesh flies is presented based on the literature, on material collected from 2004 to 2017, and on specimens in museum collections. The checklist comprises 22 genera and 148 species (two left unnamed), 105 of which are represented by new Croatian records. Twenty-five species are recorded from Croatia with certainty for the first time: Amobia pelopei (Rondani, 1859), Apodacra seriemaculata Macquart, 1854, Craticulina tabaniformis (Fabricius, 1805), Macronychia striginervis (Zetterstedt, 1838), Metopia campestris (Fallén, 1810), Miltogramma brevipila Villeneuve, 1911, Miltogramma iberica Villeneuve, 1912, Miltogramma oestracea (Fallén, 1820), Miltogramma punctata Meigen, 1824, Oebalia cylindrica (Fallén, 1810), Phylloteles pictipennis Loew, 1844, Senotainia conica (Fallén, 1810), Taxigramma hilarella (Zetterstedt, 1844), Taxigramma stictica (Meigen, 1830), Agria monachae (Kramer, 1908), Nyctia lugubris (Macquart, 1843), Blaesoxipha (Blaesoxipha) aurulenta Rohdendorf, 1937, Blaesoxipha (Blaesoxipha) batilligera Séguy, 1941, Blaesoxipha (Blaesoxipha) plumicornis (Zetterstedt, 1859), Sarcophaga (Helicophagella) okaliana (Lehrer, 1975), Sarcophaga (Heteronychia) amita Rondani, 1860, Sarcophaga (Heteronychia) ancilla Rondani, 1865, Sarcophaga (Heteronychia) pseudobenaci (Baranov, 1942), Sarcophaga (Myorhina) lunigera Böttcher, 1914 and Sarcophaga (Stackelbergeola) mehadiensis Böttcher, 1912. Taxigramma hilarella, Nyctia lugubris, Agria monachae, Blaesoxipha (Blaesoxipha) aurulenta and Sarcophaga (Heteronychia) amita are recorded from Southeast Europe with certainty for the first time. The species Sarcophaga (Sarcophaga) hennigi Lehrer, 1978 is omitted from the list, as previous records from Croatia are shown to be based on an erroneous synonymy with Sarcophaga novaki Baranov, 1941 (= Sarcophaga (Sarcophaga) croatica Baranov, 1941). Blaesoxipha (Blaesoxipha) rufipes (Macquart, 1839) could not be confirmed from Croatia and is not included in the checklist. Three new synonymies are proposed: Golania Lehrer, 2000 = Thyrsocnema Enderlein, 1928, syn. nov., ParaSarcophaga (LioSarcophaga) kovatschevitchi Strukan, 1970 = Sarcophaga (LioSarcophaga) marshalli Parker, 1923, syn. nov., and Sarcophaga subvicina ssp. novaki Baranov, 1941 = Sarcophaga (Sarcophaga) croatica Baranov, 1941, syn. nov. As part of an effort to update the European distributions of all Croatian species, the following new national and regional records are also provided: Miltogramma brevipila, Miltogramma taeniata Meigen, 1824 and Sarcophaga (Heteronychia) pandellei (Rohdendorf, 1937) new to Greece; Sarcophaga (LioSarcophaga) harpax Pandellé, 1896 and Sarcophaga (Sarcophaga) croatica new to Italy (respectively mainland and mainland and Sicily); Miltogramma iberica new to Bulgaria and Sardinia; Pterella convergens (Pandellé, 1895) new to mainland Italy and Sicily; Nyctia lugubris new to mainland Italy and Sardinia; Blaesoxipha (Blaesoxipha) litoralis (Villeneuve, 1911) new to Sardinia and thus confirmed for Italy; Apodacra seriemaculata, Macronychia striginervis, Protomiltogramma fasciata (Meigen, 1824) and Blaesoxipha (Blaesoxipha) ungulata (Pandellé, 1896) new to Sardinia and Sicily; Macronychia dolini Verves & Khrokalo, 2006, Macronychia polyodon (Meigen, 1824), Metopia argyrocephala (Meigen, 1824), Senotainia albifrons (Rondani, 1859), Taxigramma multipunctata (Rondani, 1859), Taxigramma stictica, Blaesoxipha (Blaesoxipha) unicolor (Villeneuve, 1912) and Sarcophaga (Helicophagella) agnata Rondani, 1860 new to Sardinia; Metopodia pilicornis (Pandellé, 1895), Miltogramma oestracea, Miltogramma rutilans Meigen, 1824, Nyctia halterata (Panzer, 1798), Blaesoxipha (Blaesoxipha) lapidosa Pape, 1994 and Blaesoxipha (Blaesoxipha) plumicornis new to Sicily
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molecular phylogeny of the hyperdiverse genus Sarcophaga diptera sarcophagidae and comparison between algorithms for identification of rogue taxa
Cladistics, 2017Co-Authors: Eliana Buenaventura, Daniel Whitmore, Thomas PapeAbstract:The hyperdiverse genus Sarcophaga Meigen, with about 890 valid species arranged within 169 subgenera, accounts for almost half of the diversity of the subfamily Sarcophaginae. Current phylogenetic hypotheses for this genus are poorly supported or based on small taxon sets, or both. Here, we use molecular data from the genes COI and 28S to reconstruct the phylogeny of Sarcophaga based on the most comprehensive sampling for the group to date: 144 species from 47 subgenera, including representatives from all regional faunas for the first time. Of the total sequences of Sarcophaga used in the present study, 94.7% were newly generated. The secondary structure of the D1–D3 expansion segments of 28S is presented for the first time for the family Sarcophagidae, and is used in a multiple sequence alignment. Branch support and tree resolution increased remarkably through rogue taxa identification and exclusion. Rogue behaviour was explained mostly as a missing data problem. The RogueNaRok web service and the algorithms chkmoves, IterPCR and prunmajor implemented in the computer program TNT were equally good at identifying critical rogue species, but chkmoves and IterPCR also identified rogue clades. Pruning rogues increased the number of monophyletic subgenera in consensus trees from one to six out of 19 subgenera with more than one representative species. Bayesian inference, maximum-likelihood and parsimony analyses recovered more monophyletic subgenera after the removal of rogue taxa, with parsimony showing the largest improvements in branch support and resolution. Although with low support, Nearctic taxa were found to be the earliest diverging lineages, followed by a subsequent diversification of Old World faunas, which is in agreement with currently available evidence of a New World origin and early diversification of Sarcophaga.
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multilocus and multiregional phylogeny reconstruction of the genus Sarcophaga diptera sarcophagidae
Molecular Phylogenetics and Evolution, 2017Co-Authors: Eliana Buenaventura, Thomas PapeAbstract:Abstract The flesh-fly genus Sarcophaga is extremely diverse and contains ca. 30% of the species in the family Sarcophagidae (∼3000 species). The phylogenetic position of the genus-group taxa Helicobia , Lipoptilocnema , and Peckia remains uncertain with respect to the hyperdiverse Sarcophaga , due to conflicting phylogenetic trees and insufficient sampling in recent studies. We present maximum-likelihood and Bayesian phylogenetic analyses of 145 species of 48 subgenera of the genus Sarcophaga from all biogeographic regions based on the molecular markers COI, 28 D1–D3 expansion regions, EF1α, and white. Our analyses find ( Lipoptilocnema + Peckia ) as the sister group of the monophyletic Sarcophaga . The genus Helicobia is placed outside Sarcophaga . Our hypotheses suggest that the ancestor shared by Sarcophaga and its sister clade originated in the Neotropical region, and the subsequent range expansion might be related to the formation of the Isthmus of Panama. This study supports the monophyly of most of the subgenera of Sarcophaga included here, and it shows the evolution of this genus to be a rapid radiation occurring in the Nearctic region with a subsequent dispersal into the Old World. The subgeneric clusters within Sarcophaga are in agreement with the current classification, with only Mauritiella , Rosellea , Helicophagella , LioSarcophaga , and Sarcorohdendorfia being non-monophyletic. We also validate the monotypic condition of 10 subgenera.
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molecules and morphology unite Sarcophaga stackelbergeola rohdendorf and s rohdendorfisca grunin within megadiverse Sarcophaga meigen sensu lato diptera sarcophagidae
Invertebrate Systematics, 2016Co-Authors: Ming Zhang, Eliana Buenaventura, Thomas Pape, Dong ZhangAbstract:The largest genus of the family Sarcophagidae (Insecta, Diptera), Sarcophaga Meigen (sensu lato), has ∼160 subgenera; however, the validity and phylogenetic relationships of these are still unclear, impeding progress in evolutionary studies. This study presents a phylogenetic hypothesis for selected subgenera of Sarcophaga s.l. based on COI sequences (685 bp) for 87 species representing 27 valid subgenera. The subgenera Stackelbergeola Rohdendorf and Rohdendorfisca Grunin are reconsidered in the light of new molecular, morphological and biological data. The female is described for the first time for a representative of both subgenera, and Sarcophaga (Rohdendorfisca) flagellifera (Grunin) is shown to be a parasitoid of tettigoniid grasshoppers. As the male of Sarcophaga (Stackelbergeola) sushkini (Rohdendorf) is insufficiently documented in the literature, a redescription is provided based on material from Xinjiang, thereby providing the first record of this subgenus and species from China. Detailed documentation through photographs, scanning electron microscopy and illustrations of the adult morphology is also provided. The subgenera Stackelbergeola and Rohdendorfisca are shown to be monophyletic, together forming a monophylum supported by molecular and morphological data, and they are placed in a wider phylogenetic context of the megadiverse genus Sarcophaga s.l.
Eliana Buenaventura - One of the best experts on this subject based on the ideXlab platform.
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Checklist of the Sarcophagidae (Diptera) of Croatia, with new records from Croatia and other Mediterranean countries
'Pensoft Publishers', 2019Co-Authors: Stjepan Krčmar, Thomas Pape, Daniel Whitmore, Eliana BuenaventuraAbstract:An updated checklist of Croatian flesh flies is presented based on the literature, on material collected from 2004 to 2017, and on specimens in museum collections. The checklist comprises 22 genera and 148 species (two left unnamed), 105 of which are represented by new Croatian records. Twenty-five species are recorded from Croatia with certainty for the first time: Amobia pelopei (Rondani, 1859), Apodacra seriemaculata Macquart, 1854, Craticulina tabaniformis (Fabricius, 1805), Macronychia striginervis (Zetterstedt, 1838), Metopia campestris (Fallén, 1810), Miltogramma brevipila Villeneuve, 1911, Miltogramma iberica Villeneuve, 1912, Miltogramma oestracea (Fallén, 1820), Miltogramma punctata Meigen, 1824, Oebalia cylindrica (Fallén, 1810), Phylloteles pictipennis Loew, 1844, Senotainia conica (Fallén, 1810), Taxigramma hilarella (Zetterstedt, 1844), Taxigramma stictica (Meigen, 1830), Agria monachae (Kramer, 1908), Nyctia lugubris (Macquart, 1843), Blaesoxipha (Blaesoxipha) aurulenta Rohdendorf, 1937, Blaesoxipha (Blaesoxipha) batilligera Séguy, 1941, Blaesoxipha (Blaesoxipha) plumicornis (Zetterstedt, 1859), Sarcophaga (Helicophagella) okaliana (Lehrer, 1975), Sarcophaga (Heteronychia) amita Rondani, 1860, Sarcophaga (Heteronychia) ancilla Rondani, 1865, Sarcophaga (Heteronychia) pseudobenaci (Baranov, 1942), Sarcophaga (Myorhina) lunigera Böttcher, 1914 and Sarcophaga (Stackelbergeola) mehadiensis Böttcher, 1912. Taxigramma hilarella, Nyctia lugubris, Agria monachae, Blaesoxipha (Blaesoxipha) aurulenta and Sarcophaga (Heteronychia) amita are recorded from Southeast Europe with certainty for the first time. The species Sarcophaga (Sarcophaga) hennigi Lehrer, 1978 is omitted from the list, as previous records from Croatia are shown to be based on an erroneous synonymy with Sarcophaga novaki Baranov, 1941 (= Sarcophaga (Sarcophaga) croatica Baranov, 1941). Blaesoxipha (Blaesoxipha) rufipes (Macquart, 1839) could not be confirmed from Croatia and is not included in the checklist. Three new synonymies are proposed: Golania Lehrer, 2000 = Thyrsocnema Enderlein, 1928, syn. nov., ParaSarcophaga (LioSarcophaga) kovatschevitchi Strukan, 1970 = Sarcophaga (LioSarcophaga) marshalli Parker, 1923, syn. nov., and Sarcophaga subvicina ssp. novaki Baranov, 1941 = Sarcophaga (Sarcophaga) croatica Baranov, 1941, syn. nov. As part of an effort to update the European distributions of all Croatian species, the following new national and regional records are also provided: Miltogramma brevipila, Miltogramma taeniata Meigen, 1824 and Sarcophaga (Heteronychia) pandellei (Rohdendorf, 1937) new to Greece; Sarcophaga (LioSarcophaga) harpax Pandellé, 1896 and Sarcophaga (Sarcophaga) croatica new to Italy (respectively mainland and mainland and Sicily); Miltogramma iberica new to Bulgaria and Sardinia; Pterella convergens (Pandellé, 1895) new to mainland Italy and Sicily; Nyctia lugubris new to mainland Italy and Sardinia; Blaesoxipha (Blaesoxipha) litoralis (Villeneuve, 1911) new to Sardinia and thus confirmed for Italy; Apodacra seriemaculata, Macronychia striginervis, Protomiltogramma fasciata (Meigen, 1824) and Blaesoxipha (Blaesoxipha) ungulata (Pandellé, 1896) new to Sardinia and Sicily; Macronychia dolini Verves & Khrokalo, 2006, Macronychia polyodon (Meigen, 1824), Metopia argyrocephala (Meigen, 1824), Senotainia albifrons (Rondani, 1859), Taxigramma multipunctata (Rondani, 1859), Taxigramma stictica, Blaesoxipha (Blaesoxipha) unicolor (Villeneuve, 1912) and Sarcophaga (Helicophagella) agnata Rondani, 1860 new to Sardinia; Metopodia pilicornis (Pandellé, 1895), Miltogramma oestracea, Miltogramma rutilans Meigen, 1824, Nyctia halterata (Panzer, 1798), Blaesoxipha (Blaesoxipha) lapidosa Pape, 1994 and Blaesoxipha (Blaesoxipha) plumicornis new to Sicily
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molecular phylogeny of the hyperdiverse genus Sarcophaga diptera sarcophagidae and comparison between algorithms for identification of rogue taxa
Cladistics, 2017Co-Authors: Eliana Buenaventura, Daniel Whitmore, Thomas PapeAbstract:The hyperdiverse genus Sarcophaga Meigen, with about 890 valid species arranged within 169 subgenera, accounts for almost half of the diversity of the subfamily Sarcophaginae. Current phylogenetic hypotheses for this genus are poorly supported or based on small taxon sets, or both. Here, we use molecular data from the genes COI and 28S to reconstruct the phylogeny of Sarcophaga based on the most comprehensive sampling for the group to date: 144 species from 47 subgenera, including representatives from all regional faunas for the first time. Of the total sequences of Sarcophaga used in the present study, 94.7% were newly generated. The secondary structure of the D1–D3 expansion segments of 28S is presented for the first time for the family Sarcophagidae, and is used in a multiple sequence alignment. Branch support and tree resolution increased remarkably through rogue taxa identification and exclusion. Rogue behaviour was explained mostly as a missing data problem. The RogueNaRok web service and the algorithms chkmoves, IterPCR and prunmajor implemented in the computer program TNT were equally good at identifying critical rogue species, but chkmoves and IterPCR also identified rogue clades. Pruning rogues increased the number of monophyletic subgenera in consensus trees from one to six out of 19 subgenera with more than one representative species. Bayesian inference, maximum-likelihood and parsimony analyses recovered more monophyletic subgenera after the removal of rogue taxa, with parsimony showing the largest improvements in branch support and resolution. Although with low support, Nearctic taxa were found to be the earliest diverging lineages, followed by a subsequent diversification of Old World faunas, which is in agreement with currently available evidence of a New World origin and early diversification of Sarcophaga.
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multilocus and multiregional phylogeny reconstruction of the genus Sarcophaga diptera sarcophagidae
Molecular Phylogenetics and Evolution, 2017Co-Authors: Eliana Buenaventura, Thomas PapeAbstract:Abstract The flesh-fly genus Sarcophaga is extremely diverse and contains ca. 30% of the species in the family Sarcophagidae (∼3000 species). The phylogenetic position of the genus-group taxa Helicobia , Lipoptilocnema , and Peckia remains uncertain with respect to the hyperdiverse Sarcophaga , due to conflicting phylogenetic trees and insufficient sampling in recent studies. We present maximum-likelihood and Bayesian phylogenetic analyses of 145 species of 48 subgenera of the genus Sarcophaga from all biogeographic regions based on the molecular markers COI, 28 D1–D3 expansion regions, EF1α, and white. Our analyses find ( Lipoptilocnema + Peckia ) as the sister group of the monophyletic Sarcophaga . The genus Helicobia is placed outside Sarcophaga . Our hypotheses suggest that the ancestor shared by Sarcophaga and its sister clade originated in the Neotropical region, and the subsequent range expansion might be related to the formation of the Isthmus of Panama. This study supports the monophyly of most of the subgenera of Sarcophaga included here, and it shows the evolution of this genus to be a rapid radiation occurring in the Nearctic region with a subsequent dispersal into the Old World. The subgeneric clusters within Sarcophaga are in agreement with the current classification, with only Mauritiella , Rosellea , Helicophagella , LioSarcophaga , and Sarcorohdendorfia being non-monophyletic. We also validate the monotypic condition of 10 subgenera.
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molecules and morphology unite Sarcophaga stackelbergeola rohdendorf and s rohdendorfisca grunin within megadiverse Sarcophaga meigen sensu lato diptera sarcophagidae
Invertebrate Systematics, 2016Co-Authors: Ming Zhang, Eliana Buenaventura, Thomas Pape, Dong ZhangAbstract:The largest genus of the family Sarcophagidae (Insecta, Diptera), Sarcophaga Meigen (sensu lato), has ∼160 subgenera; however, the validity and phylogenetic relationships of these are still unclear, impeding progress in evolutionary studies. This study presents a phylogenetic hypothesis for selected subgenera of Sarcophaga s.l. based on COI sequences (685 bp) for 87 species representing 27 valid subgenera. The subgenera Stackelbergeola Rohdendorf and Rohdendorfisca Grunin are reconsidered in the light of new molecular, morphological and biological data. The female is described for the first time for a representative of both subgenera, and Sarcophaga (Rohdendorfisca) flagellifera (Grunin) is shown to be a parasitoid of tettigoniid grasshoppers. As the male of Sarcophaga (Stackelbergeola) sushkini (Rohdendorf) is insufficiently documented in the literature, a redescription is provided based on material from Xinjiang, thereby providing the first record of this subgenus and species from China. Detailed documentation through photographs, scanning electron microscopy and illustrations of the adult morphology is also provided. The subgenera Stackelbergeola and Rohdendorfisca are shown to be monophyletic, together forming a monophylum supported by molecular and morphological data, and they are placed in a wider phylogenetic context of the megadiverse genus Sarcophaga s.l.
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phylogeny of the peckia genus group evolution of male genitalia in the major necrophagous guild of neotropical flesh flies diptera sarcophagidae
Organisms Diversity & Evolution, 2015Co-Authors: Eliana Buenaventura, Thomas PapeAbstract:Peckia is the most species-rich necrophagous genus among the Neotropical sarcophagids, encompassing 67 species distributed in 5 subgenera. Recent phylogenetic studies have challenged the monophyly of this genus with regard to species of the genera Peckiamyia, Titanogrypa, and Villegasia, and the genera Engelimyia, Helicobia, Retrocitomyia, and Sarcophaga. These genera have variously been hypothesized as sister groups to Peckia, or genera closely related to it. We applied cladistic methods using both molecular and morphological data to study phylogenetic relationships of these mostly necrophagous taxa. All currently recognized species of Peckia were included in our analysis. Based on 116 morphological characters and sequences of five gene fragments, we corroborate the recent division of Peckia into five subgenera, and we argue that the reduction of the acrophallic median stylus is an autapomorphic condition supporting the clade (Peckia + (Lipoptilocnema (Helicobia + Sarcophaga))). Our analysis shows that Peckiamyia is sister to Retrocitomyia, and Titanogrypa is sister to Villegasia, which together with Engelimyia form lineages that emerge in a basal divergence with regard to the clade with no median stylus. Alternative homology interpretations of the median stylus were studied and tested in a phylogenetic context. The median stylus and other phallic homologies were revisited and redefined. All studied genera were found to be monophyletic.
Shunji Natori - One of the best experts on this subject based on the ideXlab platform.
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differential activation of the lectin and antimicrobial peptide genes in Sarcophaga peregrina the flesh fly
Archives of Insect Biochemistry and Physiology, 2008Co-Authors: Shunji Natori, Takahiro Tanji, Hirohisa Shiraishi, Ayako OhashikobayashiAbstract:Sarcophaga lectin is an immune defense protein which is transcriptionally induced upon immune challenge in the flesh fly, Sarcophaga peregrina. So far, we have revealed that the Sarcophaga lectin gene has multiple NF-κB -binding motifs in its promoter. Here we showed that the nuclear extracts from Sarcophaga-derived culture cells, NIH-Sape-4, and larval fat bodies have binding activity to the multiple κB motifs in the lectin gene promoter, some of which were responsive to immune stimuli. We also compared the expression profiles of the lectin gene with those of the antibacterial peptide genes from the point of view of inducers, expression tissues and local induction in digestive tracts. In each case, the lectin gene was activated in different manners from other inducible defense genes. These results indicate the complex regulation of the lectin gene, possibly by NF-κB -related transcription factors. Arch. Insect Biochem. Physiol. 2008. © 2008 Wiley-Liss, Inc.
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a novel egg derived tyrosine phosphatase edtp that participates in the embryogenesis of Sarcophaga peregrina flesh fly
FEBS Journal, 2001Co-Authors: Shinji Yamaguchi, Koichi J Homma, Shunji NatoriAbstract:We have previously reported that cathepsin L mRNA is present in unfertilized eggs of Sarcophaga peregrina (flesh fly) as a maternal mRNA, which suggests that cathepsin L is required for embryogenesis. Now we have identified an egg protein, with a molecular mass of 100 kDa, that is extremely susceptible to cathepsin L digestion and which disappears rapidly as the embryos develop. We purified this protein to homogeneity, cloned its cDNA, and found that it contained a consensus sequence for the active site of tyrosine phosphatase. In fact this protein showed tyrosine phosphatase activity, indicating that it is a novel tyrosine phosphatase. The expression and subsequent disappearance of this protein, which we have named egg-derived tyrosine phosphatase (EDTP), may be indispensable for embryogenesis of Sarcophaga.
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the roles of Sarcophaga defense molecules in immunity and metamorphosis
Developmental and Comparative Immunology, 1999Co-Authors: Shunji Natori, Hirohisa Shiraishi, Shohei Hori, Ayako KobayashiAbstract:This article summarizes recent progress (1996–1998) in our studies on self-defense molecules in Sarcophaga peregrina. A new antibacterial substance was purified and its unique structure and function revealed a novel aspect of the Sarcophaga defense system. We found a novel lectin and cysteine protease in hemocytes which will assist in the understanding of immune response of hemocytes. There have been two major advances in research on the regulation of defense gene induction: (i) cDNA cloning of a new transcriptional factor binding to the κB-like promoter sequence of the Sarcophaga lectin gene, (ii) methylation of cytosolic factors essential for induction of immune genes in the fatbody. Metamorphosis is an interesting event from an immunological point of view: (i) a novel protease with antibacterial activity was discovered from metamorphosing gut, and (ii) a pupal hemocyte-specific surface antigen was purified and characterized in terms of its structure and possible function for larval tissue recognition and elimination.
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molecular characterization of an insect transferrin and its selective incorporation into eggs during oogenesis
FEBS Journal, 1995Co-Authors: Takeshi Kurama, Shoichiro Kurata, Shunji NatoriAbstract:A protein with a molecular mass of 65 kDa that was specifically taken up into eggs was purified from the hemolymph of adult female Sarcophaga peregrina flies. From cDNA analysis, this protein was shown to be a Sarcophaga transferrin. Unlike mammalian transferrin, the similarity between its N-terminal and C-terminal halves was only 19%, and it was suggested to conjugate one iron atom/molecule in its N-terminal half. Sarcophaga transferrin was found to transport iron ions into eggs during oogenesis and deliver them to another protein, thought to be ferritin. No significant activation of the transferrin gene was detected during embryogenesis, so probably maternal transferrin is used as an intercellular or intracellular iron-transporter during embryogenesis of this insect.
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purification and characterization of a 59 kilodalton protein that specifically binds to nf kappa b binding motifs of the defense protein genes of Sarcophaga peregrina the flesh fly
Molecular and Cellular Biology, 1993Co-Authors: A Kobayashi, M Matsui, T Kubo, Shunji NatoriAbstract:Various Sarcophaga peregrina (flesh fly) defense protein genes were shown to be activated when NIH-Sape-4 cells were cultured with bacterial lipopolysaccharides or beta-1,3-glucan. The 5' upstream regions of the defense protein genes were found to have common motifs showing similarity to the mammalian NF-kappa B-binding consensus sequence. A protein with affinity to the NF-kappa B-binding motif of the Sarcophaga lectin promoter was identified and purified to near homogeneity. This 59-kDa protein also bound to the NF-kappa B-binding motifs of other defense protein genes, e.g., sarcotoxin I and sarcotoxin II genes. This protein was found in both the cytoplasmic and the nuclear fractions of the cells, and it appeared to migrate from the cytoplasm to the nucleus on treatment of the cells with lipopolysaccharides. This 59-kDa protein is probably a transcriptional regulator of the genes for defense proteins of S. peregrina.
Stephen L. Cameron - One of the best experts on this subject based on the ideXlab platform.
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utility of coi cad and morphological data for resolving relationships within the genus Sarcophaga sensu lato diptera sarcophagidae a preliminary study
Molecular Phylogenetics and Evolution, 2013Co-Authors: Kelly A. Meiklejohn, Stephen L. Cameron, James F. Wallman, Thomas Pape, Mark DowtonAbstract:Abstract Currently there are ∼3000 known species of Sarcophagidae (Diptera), which are classified into 173 genera in three subfamilies. Almost 25% of sarcophagids belong to the genus Sarcophaga (sensu lato) however little is known about the validity of, and relationships between the ∼150 (or more) subgenera of Sarcophaga s.l. In this preliminary study, we evaluated the usefulness of three sources of data for resolving relationships between 35 species from 14 Sarcophaga s.l. subgenera: the mitochondrial COI barcode region, ∼800 bp of the nuclear gene CAD, and 110 morphological characters. Bayesian, maximum likelihood (ML) and maximum parsimony (MP) analyses were performed on the combined dataset. Much of the tree was only supported by the Bayesian and ML analyses, with the MP tree poorly resolved. The genus Sarcophaga s.l. was resolved as monophyletic in both the Bayesian and ML analyses and strong support was obtained at the species-level. Notably, the only subgenus consistently resolved as monophyletic was Liopygia. The monophyly of and relationships between the remaining Sarcophaga s.l. subgenera sampled remain questionable. We suggest that future phylogenetic studies on the genus Sarcophaga s.l. use combined datasets for analyses. We also advocate the use of additional data and a range of inference strategies to assist with resolving relationships within Sarcophaga s.l.
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the complete mitochondrial genome of the flesh fly Sarcophaga impatiens walker diptera sarcophagidae
Mitochondrial DNA, 2012Co-Authors: Leigh A Nelson, Stephen L. Cameron, David K. YeatesAbstract:Approximately 2500 fly species comprise the Sarcophagidae family worldwide. The complete mitochondrial genome of the carrion-breeding, forensically important Sarcophaga impatiens Walker (Diptera: Sarcophagidae) from Australia was sequenced. The 15,169 bp circular genome contains the 37 genes found in a typical Metazoan genome: 13 protein-coding genes, 2 ribosomal RNA genes and 22 transfer RNA genes. It also contains one non-coding A+T-rich region. The arrangement of the genes was the same as that found in the ancestral insect. All the protein initiation codons are ATN, except for cox1 that begins with TCG (encoding S). The 22 tRNA anticodons of S. impatiens are consistent with those observed in Drosophila yakuba, and all form the typical cloverleaf structure, except for tRNA-Ser(AGN) that lacks the DHU arm. The mitochondrial genome of Sarcophaga presented will be valuable for resolving phylogenetic relationships within the family Sarcophagidae and the order Diptera, and could be used to identify favourabl...
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the complete mitochondrial genome of the flesh fly Sarcophaga impatiens walker diptera sarcophagidae
Science & Engineering Faculty, 2012Co-Authors: Leigh A Nelson, Stephen L. Cameron, David K. YeatesAbstract:Approximately 2500 fly species comprise the Sarcophagidae family worldwide. The complete mitochondrial genome of the carrion-breeding, forensically important Sarcophaga impatiens Walker (Diptera: Sarcophagidae) from Australia was sequenced. The 15,169 bp circular genome contains the 37 genes found in a typical Metazoan genome: 13 protein-coding genes, 2 ribosomal RNA genes and 22 transfer RNA genes. It also contains one non-coding A+T-rich region. The arrangement of the genes was the same as that found in the ancestral insect. All the protein initiation codons are ATN, except for cox1 that begins with TCG (encoding S). The 22 tRNA anticodons of S. impatiens are consistent with those observed in Drosophila yakuba, and all form the typical cloverleaf structure, except for tRNA-Ser(AGN) that lacks the DHU arm. The mitochondrial genome of Sarcophaga presented will be valuable for resolving phylogenetic relationships within the family Sarcophagidae and the order Diptera, and could be used to identify favourable genetic markers for species identifications for forensic purposes.
Daniel Whitmore - One of the best experts on this subject based on the ideXlab platform.
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Checklist of the Sarcophagidae (Diptera) of Croatia, with new records from Croatia and other Mediterranean countries
'Pensoft Publishers', 2019Co-Authors: Stjepan Krčmar, Thomas Pape, Daniel Whitmore, Eliana BuenaventuraAbstract:An updated checklist of Croatian flesh flies is presented based on the literature, on material collected from 2004 to 2017, and on specimens in museum collections. The checklist comprises 22 genera and 148 species (two left unnamed), 105 of which are represented by new Croatian records. Twenty-five species are recorded from Croatia with certainty for the first time: Amobia pelopei (Rondani, 1859), Apodacra seriemaculata Macquart, 1854, Craticulina tabaniformis (Fabricius, 1805), Macronychia striginervis (Zetterstedt, 1838), Metopia campestris (Fallén, 1810), Miltogramma brevipila Villeneuve, 1911, Miltogramma iberica Villeneuve, 1912, Miltogramma oestracea (Fallén, 1820), Miltogramma punctata Meigen, 1824, Oebalia cylindrica (Fallén, 1810), Phylloteles pictipennis Loew, 1844, Senotainia conica (Fallén, 1810), Taxigramma hilarella (Zetterstedt, 1844), Taxigramma stictica (Meigen, 1830), Agria monachae (Kramer, 1908), Nyctia lugubris (Macquart, 1843), Blaesoxipha (Blaesoxipha) aurulenta Rohdendorf, 1937, Blaesoxipha (Blaesoxipha) batilligera Séguy, 1941, Blaesoxipha (Blaesoxipha) plumicornis (Zetterstedt, 1859), Sarcophaga (Helicophagella) okaliana (Lehrer, 1975), Sarcophaga (Heteronychia) amita Rondani, 1860, Sarcophaga (Heteronychia) ancilla Rondani, 1865, Sarcophaga (Heteronychia) pseudobenaci (Baranov, 1942), Sarcophaga (Myorhina) lunigera Böttcher, 1914 and Sarcophaga (Stackelbergeola) mehadiensis Böttcher, 1912. Taxigramma hilarella, Nyctia lugubris, Agria monachae, Blaesoxipha (Blaesoxipha) aurulenta and Sarcophaga (Heteronychia) amita are recorded from Southeast Europe with certainty for the first time. The species Sarcophaga (Sarcophaga) hennigi Lehrer, 1978 is omitted from the list, as previous records from Croatia are shown to be based on an erroneous synonymy with Sarcophaga novaki Baranov, 1941 (= Sarcophaga (Sarcophaga) croatica Baranov, 1941). Blaesoxipha (Blaesoxipha) rufipes (Macquart, 1839) could not be confirmed from Croatia and is not included in the checklist. Three new synonymies are proposed: Golania Lehrer, 2000 = Thyrsocnema Enderlein, 1928, syn. nov., ParaSarcophaga (LioSarcophaga) kovatschevitchi Strukan, 1970 = Sarcophaga (LioSarcophaga) marshalli Parker, 1923, syn. nov., and Sarcophaga subvicina ssp. novaki Baranov, 1941 = Sarcophaga (Sarcophaga) croatica Baranov, 1941, syn. nov. As part of an effort to update the European distributions of all Croatian species, the following new national and regional records are also provided: Miltogramma brevipila, Miltogramma taeniata Meigen, 1824 and Sarcophaga (Heteronychia) pandellei (Rohdendorf, 1937) new to Greece; Sarcophaga (LioSarcophaga) harpax Pandellé, 1896 and Sarcophaga (Sarcophaga) croatica new to Italy (respectively mainland and mainland and Sicily); Miltogramma iberica new to Bulgaria and Sardinia; Pterella convergens (Pandellé, 1895) new to mainland Italy and Sicily; Nyctia lugubris new to mainland Italy and Sardinia; Blaesoxipha (Blaesoxipha) litoralis (Villeneuve, 1911) new to Sardinia and thus confirmed for Italy; Apodacra seriemaculata, Macronychia striginervis, Protomiltogramma fasciata (Meigen, 1824) and Blaesoxipha (Blaesoxipha) ungulata (Pandellé, 1896) new to Sardinia and Sicily; Macronychia dolini Verves & Khrokalo, 2006, Macronychia polyodon (Meigen, 1824), Metopia argyrocephala (Meigen, 1824), Senotainia albifrons (Rondani, 1859), Taxigramma multipunctata (Rondani, 1859), Taxigramma stictica, Blaesoxipha (Blaesoxipha) unicolor (Villeneuve, 1912) and Sarcophaga (Helicophagella) agnata Rondani, 1860 new to Sardinia; Metopodia pilicornis (Pandellé, 1895), Miltogramma oestracea, Miltogramma rutilans Meigen, 1824, Nyctia halterata (Panzer, 1798), Blaesoxipha (Blaesoxipha) lapidosa Pape, 1994 and Blaesoxipha (Blaesoxipha) plumicornis new to Sicily
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molecular phylogeny of the hyperdiverse genus Sarcophaga diptera sarcophagidae and comparison between algorithms for identification of rogue taxa
Cladistics, 2017Co-Authors: Eliana Buenaventura, Daniel Whitmore, Thomas PapeAbstract:The hyperdiverse genus Sarcophaga Meigen, with about 890 valid species arranged within 169 subgenera, accounts for almost half of the diversity of the subfamily Sarcophaginae. Current phylogenetic hypotheses for this genus are poorly supported or based on small taxon sets, or both. Here, we use molecular data from the genes COI and 28S to reconstruct the phylogeny of Sarcophaga based on the most comprehensive sampling for the group to date: 144 species from 47 subgenera, including representatives from all regional faunas for the first time. Of the total sequences of Sarcophaga used in the present study, 94.7% were newly generated. The secondary structure of the D1–D3 expansion segments of 28S is presented for the first time for the family Sarcophagidae, and is used in a multiple sequence alignment. Branch support and tree resolution increased remarkably through rogue taxa identification and exclusion. Rogue behaviour was explained mostly as a missing data problem. The RogueNaRok web service and the algorithms chkmoves, IterPCR and prunmajor implemented in the computer program TNT were equally good at identifying critical rogue species, but chkmoves and IterPCR also identified rogue clades. Pruning rogues increased the number of monophyletic subgenera in consensus trees from one to six out of 19 subgenera with more than one representative species. Bayesian inference, maximum-likelihood and parsimony analyses recovered more monophyletic subgenera after the removal of rogue taxa, with parsimony showing the largest improvements in branch support and resolution. Although with low support, Nearctic taxa were found to be the earliest diverging lineages, followed by a subsequent diversification of Old World faunas, which is in agreement with currently available evidence of a New World origin and early diversification of Sarcophaga.
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phylogeny of heteronychia the largest lineage of Sarcophaga diptera sarcophagidae
Zoological Journal of the Linnean Society, 2013Co-Authors: Daniel Whitmore, Thomas Pape, Pierfilippo CerrettiAbstract:Sarcophaga Meigen is one of the megadiverse genera of true flies, with approximately 850 valid species worldwide. The genus is divided into about 160 subgenera, the validity of a vast majority of which has never been verified using cladistic methods. This paper deals with the mainly Palaearctic subgenus Heteronychia Brauer & Bergenstamm, which comprises 89 species and is thus the largest subunit of Sarcophaga. We performed a cladistic analysis of the group based exclusively on male morphological characters. Parsimony analyses were run on a matrix of 84 characters for 88 species. Species of the subgenera Discachaeta Enderlein and Notoecus Stein were also included in the matrix. A further analysis was carried out using a subset of characters from the terminalia alone (70 characters). The results show that the clade formed by Heteronychia, Discachaeta, and Notoecus is monophyletic, with Discachaeta emerging as polyphyletic whereas Sarcophaga (Notoecus) longestylata Strobl is nested within the Sarcophaga filia-group. Character states supporting Heteronychia and the few well-supported species-groups are discussed in detail. The following synonymies are proposed: Discachaeta = Heteronychia (syn. nov.) and Notoecus = Heteronychia (syn. nov.). The paper also includes a historical background of the taxon in relation to the classification of the genus Sarcophaga over the past two centuries, as well as a terminological review of the male terminalia, particularly of the distiphallus.
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new taxonomic and nomenclatural data on Sarcophaga heteronychia diptera sarcophagidae with description of six new species
Zootaxa, 2011Co-Authors: Daniel WhitmoreAbstract:This paper presents new data on Sarcophaga (Heteronychia). Six species are described as new: Sarcophaga (Heterony- chia) anatolica sp. nov. from Asiatic Turkey, S. (H.) lejlekensis sp. nov. from Kyrgyzstan, S. (H.) mediterranea sp. nov. from mainland Italy, Sicily and Croatia, S. (H.) rosellensis sp. nov. from central Italy, S. (H.) tetrix sp. nov. from Sicily, and S. (H.) tunisiae sp. nov. from Tunisia. Two new replacement names are proposed: Sarcophaga (Heteronychia) hellenica, nom. nov. for S. (H.) vervesi (Povolný, 1996) (junior secondary homonym of Kozlovea vervesi Nandi, 1993), and S. (H.) tangerensis, nom. nov. for S. (H.) amica (Peris et al., 1998) (junior secondary homonym of Phallosphaera amica Ma, 1964). Sarcophaga (Heteronychia) belanovskyi (Verves, 1973), S. (H.) helenae (Trofimov, 1948) and S. (H.) nanula (Povolný, 1999) are resurrected as valid, and the last (originally proposed as a subspecies) is given species rank. Sarcophaga amita Rondani, 1860 and S. bezziana Bottcher, 1913 are transferred from subgenus Discachaeta Enderlein to subgenus Heteronychia Brauer & Bergenstamm, subg. comb. nov. Thirty-nine new synonymies are established: Shoachaeta Lehrer, 1997 with Heteronychia Brauer & Bergenstamm, 1889, syn. nov.; Ashlaiana Lehrer, 1998 with Heteronychia Brauer & Bergenstamm, 1889, syn. nov.; Heteronychia iubita Lehrer, 1999 with Sarcophaga (Heteronychia) ancilla Rondani, 1865, syn. nov.; Spatulapica lucentina Lehrer & Martinez-Sanchez, 2001 with S. (H.) ancilla Rondani, 1865, syn. nov.; Heteronychia (Heteronychia) povolnyi Mihalyi, 1975 with S. (H.) belanovskyi (Verves, 1973), syn. nov.; Sarcophaga benaci var. tenuiforceps Bottcher, 1913 with S. (H.) benaci Bottcher, 1913, syn. nov.; Heteronychia (Heteronychia) vachai Povolný, 1987 with S. (H.) benaci Bottcher, 1913, syn. nov.; Shoachaeta cornogranda Lehrer, 2009 with S. (H.) bezziana Bottcher, 1913, syn. nov.; Pierretia (Pandelleola) taurica Rohdendorf, 1937 with S. (H.) boettcheri Villeneuve, 1912, syn. nov.; Heteronychia (Pandelleola) gaspari Lehrer, 1977 with S. (H.) boettcheri Villeneuve, 1912, syn. nov.; Heteronychia bodediana Lehrer, 1998 with S. (H.) boettcheri Villeneuve, 1912, syn. nov.; Ashlaiana shakrana Lehrer, 1998 with S. (H.) boettcheri Villeneuve, 1912, syn. nov.; Pandelleola caraormana Lehrer, 2008 with S. (H.) boettcheri Villeneuve, 1912, syn. nov.; Heteronychia (Heteronychia) morenita Peris, Gonzalez-Mora & Mingo, 1998 with S. (H.) chiquita (Peris, Gonzalez-Mora & Mingo, 1998), syn. nov.; Heteronychia cullottorum Povolný, 2005 with S. (H.) consanguinea Rondani, 1860, syn. nov.; Heteronychia (Eupierretia) spatulifera Chen & Lu, 1981 with S. (H.) curvifemoralis (Li, 1980), syn. nov.; Heteronychia (Spatulapica) kovalae Verves, 1979 with S. (H.) depressifrons Zetterstedt, 1845, syn. nov.; Heteronychia dimioniphalla Lehrer, 1996 with S. (H.) dissimilis Meigen, 1826, syn. nov.; Heteronychia (Eupierretia) macedonica Povolný, 1996 with S. (H.) enderleini Jacentkovský, 1937, syn. nov.; Devriesia weberi Lehrer, 1995 with S. (H.) ferox Villeneuve, 1908, syn. nov.; Heteronychia (As- celoctis) perplexa Peris, Gonzalez-Mora & Mingo, 1996 with S. (H.) ferox Villeneuve, 1908, syn. nov.; Pandelleola resnikae Lehrer, 1996 with S. (H.) filia Rondani, 1860, syn. nov.; Heteronychia fugitiva Povolný, 2001 with S. (H.) haemorrhoa Meigen, 1826, syn. nov.; Heteronychia (Spatulapica) fraterna Lehrer, 1977 with S. (H.) helenae (Trofimov, 1948), syn. nov.; Spatulapica delicata Lehrer, 2000 with S. (H.) helenae (Trofimov, 1948), syn. nov.; Heteronychia dayani Lehrer, 1996 with S. (H.) kerteszi Villeneuve, 1912, syn. nov.; Salemea sororia Povolný, 2004 with S. (H.) kerteszi Villeneuve, 1912, syn. nov.; Heteronychia (Eupierretia) helanshanensis Han, Chao & Ye, 1985 with S. (H.) kozlovi (Rohdendorf, 1937), syn. nov.; Sarcophaga thalhammeri Bottcher, 1913 with S. (H.) lacrymans Villeneuve, 1912, syn. nov.; Sarcophaga zhelochovtzevi Rohdendorf, 1925 with S. (H.) lacrymans Villeneuve, 1912, syn. nov.; Heteronychia (Heter- onychia) cepelaki Povolný & Slameckova, 1970 with S. (H.) lacrymans Villeneuve, 1912, syn. nov.; Heteronychia histriops Lehrer, 2008 with S. (H.) lacrymans Villeneuve, 1912, syn. nov.; Heteronychia (Eupierretia) brachystylata Chao & Zhang, 1988 with S. (H.) plotnikovi Rohdendorf, 1925, syn. nov.; Heteronychia lednicensis Povolný in Povolný & Verves, 1986 with S. (H.) proxima Rondani, 1860, syn. nov.; Heteronychia (Heteronychia) drenskiana Lehrer, 1977 with S. (H.) pseudobenaci (Baranov, 1942), syn. nov.; Heteronychia (Eupierretia) peckae Verves, 1977 with S. (H.) shnitnikovi (Roh-dendorf, 1937), syn. nov.; Heteronychia (Eupierretia) tenupenialis Chao & Zhang, 1988 with S. (H.) shnitnikovi (Rohdendorf, 1937), syn. nov.; Heteronychia (Pandelleola) volcanoaetnica Povolný, 2002 with S. (H.) sicilia Pape, 1996, syn. nov.; Hartigia anastrenua Baranov, 1942 with S. (H.) vagans Meigen, 1826, syn. nov. Lectotypes are designated for the following nominal taxa: Mehria pseudobenaci Baranov, 1942, Pierretia (Pierretia) boettcheriana Rohdendorf, 1937, Pierretia (Pierretia) obscurata Rohdendorf, 1937, Sarcophaga benaci Bottcher, 1913, Sarcophaga benaci var. tenuiforceps Bottcher, 1913, Sarcophaga boettcheri Villeneuve, 1912, Sarcophaga ferox Villeneuve, 1908, Sarcophaga fertoni Villeneuve, 1911, Sarcophaga kerteszi Villeneuve, 1912, Sarcophaga lacrymans Villeneuve, 1912, Sarcophaga metopina Villeneuve, 1908, Sarcophaga monspellensia Bottcher, 1913, Sarcophaga porrecta Bottcher, 1913, Sarcophaga setinervis var. mutila Villeneuve, 1912, Sarcophaga thalhammeri Bottcher, 1913 and Sarcophaga tricolor Villeneuve, 1908. Sarcophaga smithiana Pape, 1996 is removed from subgenus Heteronychia and considered as incertae sedis within Sarcophaga. New country or regional records are provided for Sarcophaga (H.) benaci (Spain), S. (H.) boettcheri (Serbia), S. (H.) croca (Croatia), S. (H.) depressifrons (Greece, Spain), S. (H.) ferox (Algeria), S. (H.) filia (Crete, Macedonia, Morocco, Tunisia), S. (H.) kataphygionis (Poland), S. (H.) kerteszi (Crete), S. (H.) minima (Crete), S. (H.) monspellensia (Sicily, Tunisia), S. (H.) mutila (Italy), S. (H.) nanula (France, Macedonia) and S. (H.) proxima (Sicily).
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a review of the Sarcophaga heteronychia diptera sarcophagidae of sardinia
Zootaxa, 2009Co-Authors: Daniel WhitmoreAbstract:An account is given of the species of Sarcophaga Meigen, 1826 subgenus Heteronychia Brauer & Bergenstamm, 1889 known from the island of Sardinia (Italy). Most of the nearly 1,400 specimens examined were collected in the SW part of the island during 2003–2006 as part of a project investigating the arthropod diversity of the Monti Marganai and Montimannu areas (respectively Carbonia-Iglesias and Medio Campidano provinces). The study resulted in the finding of eight species of Heteronychia, six of which are recorded from Sardinia for the first time. Sarcophaga (Heteronychia) penicillata Villeneuve, 1907, previously mentioned in the literature, is excluded from the fauna of the island. Sarcophaga (Heteronychia) thirionae (Lehrer, 1976) is recorded for the first time from Europe and North Africa (Algeria). One species, Sarcophaga (Heteronychia) gabrielei sp. nov., from various sites in the limestone massif of Marganai, is described as new. Previously unpublished records from other Italian regions and from other countries (Algeria, Canary Islands, Greece) are also given for several species. Sarcophaga (Heteronychia) schnabli Villeneuve, 1911 is recognized as a junior synonym of S. (H.) consanguinea Rondani, 1860 syn. nov. The possible synanthropy of Sarcophaga (Heteronychia) pandellei (Rohdendorf, 1937) is briefly discussed. A key to males and females of all known Sardinian and Corsican species of Sarcophaga (Heteronychia) is provided.
