The Experts below are selected from a list of 162 Experts worldwide ranked by ideXlab platform

Heinz L Sanger - One of the best experts on this subject based on the ideXlab platform.

  • secondary structure probing of potato spindle tuber viroid pstvd and sequence comparison with other small pathogenic rna replicons provides evidence for central non canonical base pairs large a rich loops and a terminal branch
    Journal of Molecular Biology, 1996
    Co-Authors: Frankulrich Gast, Dirk Kempe, Reiner L Spieker, Heinz L Sanger
    Abstract:

    Using PCR and in vitro transcription, linear (non-circular) unit-length (+)strand RNA molecules of a lethal PSTVd variant were produced which are able to initiate typical disease symptoms when inoculated into tomato. Non-denaturing gel electrophoresis shows that these transcripts can adopt the same two conformations as circular PSTVd molecules, namely a fast migrating rod-like and a slowly migrating cruciform structure. The rod-like conformer of two end-labelled transcripts was probed with nucleases and dimethyl sulphate, revealing that in solution its right part is identical to Computer Prediction. In the left part, however, three unique features could be substantiated. (1) In the central region a UV-cross-linkable loop is closed and thus contains non-canonical base-pairs ("loop E structure"). (2) Three large "pre-melting loops" are present at 25 degrees to 37 degrees C. The structure of the leftmost one, which is A-rich and conserved in most viroids, correlates with pathogenicity. (3) Two small stem-loops instead of an unbranched structure are found at the left terminus. These hairpins can form in all "large" viroids (approximately 300 nucleotides or longer), thus placing the dodecamer conserved among these viroids, GGUUCCUGUGGU, within the upper helix and the branch junction. A large viroid from Iresine lacks one of these hairpins, whereas all "small" viroids (approximately 300 nucleotides or smaller) lack both. In several plant virus satellite RNAs and the newt satellite RNA, the motif GAUUU(U) and dodecamer remnants appear in an equivalent structure comprising two or three hairpins. Using lead- and terbium-induced cleavage of the RNA, metal binding sites were found, mostly in loops. Thus, probing of PSTVd RNA and comparison with other.

  • secondary structure probing of potato spindle tuber viroid pstvd and sequence comparison with other small pathogenic rna replicons provides evidence for central non canonical base pairs large a rich loops and a terminal branch
    Journal of Molecular Biology, 1996
    Co-Authors: Frankulrich Gast, Dirk Kempe, Reiner L Spieker, Heinz L Sanger
    Abstract:

    Abstract Using PCR andin vitrotranscription, linear (non-circular) unit-length (+)strand RNA molecules of a lethal PSTVd variant were produced which are able to initiate typical disease symptoms when inoculated into tomato. Non-denaturing gel electrophoresis shows that these transcripts can adopt the same two conformations as circular PSTVd molecules, namely a fast migrating rod-like and a slowly migrating cruciform structure. The rod-like conformer of two end-labelled transcripts was probed with nucleases and dimethyl sulphate, revealing that in solution its right part is identical to Computer Prediction. In the left part, however, three unique features could be substantiated. (1) In the central region a UV-cross-link able loop is closed and thus contains non-canonical base-pairs (“loop E structure”). (2) Three large “pre-melting loops” are present at 25° to 37°C. The structure of the leftmost one, which is A-rich and conserved in most viroids, correlates with pathogenicity. (3) Two small stem-loops instead of an unbranched structure are found at the left terminus. These hairpins can form in all “large” viroids (≈300 nucleotides or longer), thus placing the dodecamer conserved among these viroids, GGUUCCUGUGGU, within the upper helix and the branch junction. A large viroid fromIresinelacks one of these hairpins, whereas all “small” viroids (≈300 nucleotides or smaller) lack both. In several plant virus satellite RNAs and the newt satellite RNA, the motif GAUUU(U) and dodecamer remnants appear in an equivalent structure comprising two or three hairpins. Using lead- and terbium-induced cleavage of the RNA, metal binding sites were found, mostly in loops. Thus, probing of PSTVd RNA and comparison with other viroids, virusoids, and satellite RNAs contributes to our understanding of the secondary structure of these small pathogenic RNAs.

Frankulrich Gast - One of the best experts on this subject based on the ideXlab platform.

  • secondary structure probing of potato spindle tuber viroid pstvd and sequence comparison with other small pathogenic rna replicons provides evidence for central non canonical base pairs large a rich loops and a terminal branch
    Journal of Molecular Biology, 1996
    Co-Authors: Frankulrich Gast, Dirk Kempe, Reiner L Spieker, Heinz L Sanger
    Abstract:

    Using PCR and in vitro transcription, linear (non-circular) unit-length (+)strand RNA molecules of a lethal PSTVd variant were produced which are able to initiate typical disease symptoms when inoculated into tomato. Non-denaturing gel electrophoresis shows that these transcripts can adopt the same two conformations as circular PSTVd molecules, namely a fast migrating rod-like and a slowly migrating cruciform structure. The rod-like conformer of two end-labelled transcripts was probed with nucleases and dimethyl sulphate, revealing that in solution its right part is identical to Computer Prediction. In the left part, however, three unique features could be substantiated. (1) In the central region a UV-cross-linkable loop is closed and thus contains non-canonical base-pairs ("loop E structure"). (2) Three large "pre-melting loops" are present at 25 degrees to 37 degrees C. The structure of the leftmost one, which is A-rich and conserved in most viroids, correlates with pathogenicity. (3) Two small stem-loops instead of an unbranched structure are found at the left terminus. These hairpins can form in all "large" viroids (approximately 300 nucleotides or longer), thus placing the dodecamer conserved among these viroids, GGUUCCUGUGGU, within the upper helix and the branch junction. A large viroid from Iresine lacks one of these hairpins, whereas all "small" viroids (approximately 300 nucleotides or smaller) lack both. In several plant virus satellite RNAs and the newt satellite RNA, the motif GAUUU(U) and dodecamer remnants appear in an equivalent structure comprising two or three hairpins. Using lead- and terbium-induced cleavage of the RNA, metal binding sites were found, mostly in loops. Thus, probing of PSTVd RNA and comparison with other.

  • secondary structure probing of potato spindle tuber viroid pstvd and sequence comparison with other small pathogenic rna replicons provides evidence for central non canonical base pairs large a rich loops and a terminal branch
    Journal of Molecular Biology, 1996
    Co-Authors: Frankulrich Gast, Dirk Kempe, Reiner L Spieker, Heinz L Sanger
    Abstract:

    Abstract Using PCR andin vitrotranscription, linear (non-circular) unit-length (+)strand RNA molecules of a lethal PSTVd variant were produced which are able to initiate typical disease symptoms when inoculated into tomato. Non-denaturing gel electrophoresis shows that these transcripts can adopt the same two conformations as circular PSTVd molecules, namely a fast migrating rod-like and a slowly migrating cruciform structure. The rod-like conformer of two end-labelled transcripts was probed with nucleases and dimethyl sulphate, revealing that in solution its right part is identical to Computer Prediction. In the left part, however, three unique features could be substantiated. (1) In the central region a UV-cross-link able loop is closed and thus contains non-canonical base-pairs (“loop E structure”). (2) Three large “pre-melting loops” are present at 25° to 37°C. The structure of the leftmost one, which is A-rich and conserved in most viroids, correlates with pathogenicity. (3) Two small stem-loops instead of an unbranched structure are found at the left terminus. These hairpins can form in all “large” viroids (≈300 nucleotides or longer), thus placing the dodecamer conserved among these viroids, GGUUCCUGUGGU, within the upper helix and the branch junction. A large viroid fromIresinelacks one of these hairpins, whereas all “small” viroids (≈300 nucleotides or smaller) lack both. In several plant virus satellite RNAs and the newt satellite RNA, the motif GAUUU(U) and dodecamer remnants appear in an equivalent structure comprising two or three hairpins. Using lead- and terbium-induced cleavage of the RNA, metal binding sites were found, mostly in loops. Thus, probing of PSTVd RNA and comparison with other viroids, virusoids, and satellite RNAs contributes to our understanding of the secondary structure of these small pathogenic RNAs.

Reiner L Spieker - One of the best experts on this subject based on the ideXlab platform.

  • secondary structure probing of potato spindle tuber viroid pstvd and sequence comparison with other small pathogenic rna replicons provides evidence for central non canonical base pairs large a rich loops and a terminal branch
    Journal of Molecular Biology, 1996
    Co-Authors: Frankulrich Gast, Dirk Kempe, Reiner L Spieker, Heinz L Sanger
    Abstract:

    Using PCR and in vitro transcription, linear (non-circular) unit-length (+)strand RNA molecules of a lethal PSTVd variant were produced which are able to initiate typical disease symptoms when inoculated into tomato. Non-denaturing gel electrophoresis shows that these transcripts can adopt the same two conformations as circular PSTVd molecules, namely a fast migrating rod-like and a slowly migrating cruciform structure. The rod-like conformer of two end-labelled transcripts was probed with nucleases and dimethyl sulphate, revealing that in solution its right part is identical to Computer Prediction. In the left part, however, three unique features could be substantiated. (1) In the central region a UV-cross-linkable loop is closed and thus contains non-canonical base-pairs ("loop E structure"). (2) Three large "pre-melting loops" are present at 25 degrees to 37 degrees C. The structure of the leftmost one, which is A-rich and conserved in most viroids, correlates with pathogenicity. (3) Two small stem-loops instead of an unbranched structure are found at the left terminus. These hairpins can form in all "large" viroids (approximately 300 nucleotides or longer), thus placing the dodecamer conserved among these viroids, GGUUCCUGUGGU, within the upper helix and the branch junction. A large viroid from Iresine lacks one of these hairpins, whereas all "small" viroids (approximately 300 nucleotides or smaller) lack both. In several plant virus satellite RNAs and the newt satellite RNA, the motif GAUUU(U) and dodecamer remnants appear in an equivalent structure comprising two or three hairpins. Using lead- and terbium-induced cleavage of the RNA, metal binding sites were found, mostly in loops. Thus, probing of PSTVd RNA and comparison with other.

  • secondary structure probing of potato spindle tuber viroid pstvd and sequence comparison with other small pathogenic rna replicons provides evidence for central non canonical base pairs large a rich loops and a terminal branch
    Journal of Molecular Biology, 1996
    Co-Authors: Frankulrich Gast, Dirk Kempe, Reiner L Spieker, Heinz L Sanger
    Abstract:

    Abstract Using PCR andin vitrotranscription, linear (non-circular) unit-length (+)strand RNA molecules of a lethal PSTVd variant were produced which are able to initiate typical disease symptoms when inoculated into tomato. Non-denaturing gel electrophoresis shows that these transcripts can adopt the same two conformations as circular PSTVd molecules, namely a fast migrating rod-like and a slowly migrating cruciform structure. The rod-like conformer of two end-labelled transcripts was probed with nucleases and dimethyl sulphate, revealing that in solution its right part is identical to Computer Prediction. In the left part, however, three unique features could be substantiated. (1) In the central region a UV-cross-link able loop is closed and thus contains non-canonical base-pairs (“loop E structure”). (2) Three large “pre-melting loops” are present at 25° to 37°C. The structure of the leftmost one, which is A-rich and conserved in most viroids, correlates with pathogenicity. (3) Two small stem-loops instead of an unbranched structure are found at the left terminus. These hairpins can form in all “large” viroids (≈300 nucleotides or longer), thus placing the dodecamer conserved among these viroids, GGUUCCUGUGGU, within the upper helix and the branch junction. A large viroid fromIresinelacks one of these hairpins, whereas all “small” viroids (≈300 nucleotides or smaller) lack both. In several plant virus satellite RNAs and the newt satellite RNA, the motif GAUUU(U) and dodecamer remnants appear in an equivalent structure comprising two or three hairpins. Using lead- and terbium-induced cleavage of the RNA, metal binding sites were found, mostly in loops. Thus, probing of PSTVd RNA and comparison with other viroids, virusoids, and satellite RNAs contributes to our understanding of the secondary structure of these small pathogenic RNAs.

Dirk Kempe - One of the best experts on this subject based on the ideXlab platform.

  • secondary structure probing of potato spindle tuber viroid pstvd and sequence comparison with other small pathogenic rna replicons provides evidence for central non canonical base pairs large a rich loops and a terminal branch
    Journal of Molecular Biology, 1996
    Co-Authors: Frankulrich Gast, Dirk Kempe, Reiner L Spieker, Heinz L Sanger
    Abstract:

    Using PCR and in vitro transcription, linear (non-circular) unit-length (+)strand RNA molecules of a lethal PSTVd variant were produced which are able to initiate typical disease symptoms when inoculated into tomato. Non-denaturing gel electrophoresis shows that these transcripts can adopt the same two conformations as circular PSTVd molecules, namely a fast migrating rod-like and a slowly migrating cruciform structure. The rod-like conformer of two end-labelled transcripts was probed with nucleases and dimethyl sulphate, revealing that in solution its right part is identical to Computer Prediction. In the left part, however, three unique features could be substantiated. (1) In the central region a UV-cross-linkable loop is closed and thus contains non-canonical base-pairs ("loop E structure"). (2) Three large "pre-melting loops" are present at 25 degrees to 37 degrees C. The structure of the leftmost one, which is A-rich and conserved in most viroids, correlates with pathogenicity. (3) Two small stem-loops instead of an unbranched structure are found at the left terminus. These hairpins can form in all "large" viroids (approximately 300 nucleotides or longer), thus placing the dodecamer conserved among these viroids, GGUUCCUGUGGU, within the upper helix and the branch junction. A large viroid from Iresine lacks one of these hairpins, whereas all "small" viroids (approximately 300 nucleotides or smaller) lack both. In several plant virus satellite RNAs and the newt satellite RNA, the motif GAUUU(U) and dodecamer remnants appear in an equivalent structure comprising two or three hairpins. Using lead- and terbium-induced cleavage of the RNA, metal binding sites were found, mostly in loops. Thus, probing of PSTVd RNA and comparison with other.

  • secondary structure probing of potato spindle tuber viroid pstvd and sequence comparison with other small pathogenic rna replicons provides evidence for central non canonical base pairs large a rich loops and a terminal branch
    Journal of Molecular Biology, 1996
    Co-Authors: Frankulrich Gast, Dirk Kempe, Reiner L Spieker, Heinz L Sanger
    Abstract:

    Abstract Using PCR andin vitrotranscription, linear (non-circular) unit-length (+)strand RNA molecules of a lethal PSTVd variant were produced which are able to initiate typical disease symptoms when inoculated into tomato. Non-denaturing gel electrophoresis shows that these transcripts can adopt the same two conformations as circular PSTVd molecules, namely a fast migrating rod-like and a slowly migrating cruciform structure. The rod-like conformer of two end-labelled transcripts was probed with nucleases and dimethyl sulphate, revealing that in solution its right part is identical to Computer Prediction. In the left part, however, three unique features could be substantiated. (1) In the central region a UV-cross-link able loop is closed and thus contains non-canonical base-pairs (“loop E structure”). (2) Three large “pre-melting loops” are present at 25° to 37°C. The structure of the leftmost one, which is A-rich and conserved in most viroids, correlates with pathogenicity. (3) Two small stem-loops instead of an unbranched structure are found at the left terminus. These hairpins can form in all “large” viroids (≈300 nucleotides or longer), thus placing the dodecamer conserved among these viroids, GGUUCCUGUGGU, within the upper helix and the branch junction. A large viroid fromIresinelacks one of these hairpins, whereas all “small” viroids (≈300 nucleotides or smaller) lack both. In several plant virus satellite RNAs and the newt satellite RNA, the motif GAUUU(U) and dodecamer remnants appear in an equivalent structure comprising two or three hairpins. Using lead- and terbium-induced cleavage of the RNA, metal binding sites were found, mostly in loops. Thus, probing of PSTVd RNA and comparison with other viroids, virusoids, and satellite RNAs contributes to our understanding of the secondary structure of these small pathogenic RNAs.

Khrustalev V.n. - One of the best experts on this subject based on the ideXlab platform.

  • One-pot synthesis of (1 RS,21 SR)-diethyl 2-[23-amino-22-ethoxycarbonyl-8,11,14-trioxa-25-azatetracyclo[19.3.1.0 2,7.0 15,20]pentacosa-2,4,6,15(20),16,18,22-heptaen-25-yl]but-2-endioate
    'International Union of Crystallography (IUCr)', 2020
    Co-Authors: Tran T.t.v., Le T.a., Truong H.h., Dao T.n., Soldatenkov A.t., Khrustalev V.n.
    Abstract:

    The title compound, C30 H34 N2 O9 (4), is a product of the Michael reaction of azacrown ether with dimethyl acetylenedicarboxylate modified by an addition of NH 3 (aq.) at 298 K. The aza-14-crown-4-ether ring adopts a bowl conformation. The dihedral angle between the planes of the benzene rings fused to the aza-14-crown-4-ether moiety is 8.65 (5)°. The tetrahydropyridine ring has a boat conformation. The molecular conformation is supported by one N-H..O and two C-H..O intramolecular hydrogen bonds. Both heterocyclic and amino N atoms have essentially planar configurations (sums of the bond angles are 359.35 and 358.00°). Compound 4 crystallizes as a racemate consisting of enantiomeric pairs of the 1R,21S diastereomer. In the crystal, molecules of 4 are connected by N-H..O hydrogen bonds, forming chains along [100]. According to the PASS program (Computer Prediction of biological activities), compound 4 may exhibit antiallergic (72% probability) and antiasthmatic (67%) activity, as well as be a membrane permeability inhibitor (65%). © 2018 Tran et al