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Petr Kubáň - One of the best experts on this subject based on the ideXlab platform.
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New approach for cystic fibrosis diagnosis based on chloride/potassium ratio analyzed in non-invasively obtained skin-wipe sweat samples by capillary electrophoresis with contactless Conductometric Detection
Analytical and Bioanalytical Chemistry, 2017Co-Authors: Pavol Ďurč, František Foret, Eva Pokojová, Lukáš Homola, Jana Skřičková, Vladimír Herout, Milan Dastych, Hana Vinohradská, Petr KubáňAbstract:A new approach for sweat analysis used in cystic fibrosis (CF) diagnosis is proposed. It consists of a noninvasive skin-wipe sampling followed by analysis of target ions using capillary electrophoresis with contactless Conductometric Detection (C4D). The skin-wipe sampling consists of wiping a defined skin area with precleaned cotton swab moistened with 100 μL deionized water. The skin-wipe sample is then extracted for 3 min into 400 μL deionized water, and the extract is analyzed directly. The developed sampling method is cheap, simple, fast, and painless, and can replace the conventional pilocarpine-induced sweat chloride test commonly applied in CF diagnosis. The aqueous extract of the skin-wipe sample content is analyzed simultaneously by capillary electrophoresis with contactless Conductometric Detection using a double opposite end injection. A 20 mmol/L l -histidine/2-( N -morpholino)ethanesulfonic acid and 2 mmol/L 18-crown-6 at pH 6 electrolyte can separate all the major ions in less than 7 min. Skin-wipe sample extracts from 30 study participants—ten adult patients with CF (25–50 years old), ten pediatric patients with CF (1–15 years old), and ten healthy control individuals (1–18 years old)—were obtained and analyzed. From the analyzed ions in all samples, a significant difference between chloride and potassium concentrations was found in the CF patients and healthy controls. We propose the use of the Cl^-/K^+ ratio rather than the absolute Cl^- concentration and a cutoff value of 4 in skin-wipe sample extracts as an alternative to the conventional sweat chloride analysis. The proposed Cl^-/K^+ ion ratio proved to be a more reliable indicator, is independent of the patient’s age, and allows better differentiation between non-CF individuals and CF patients having intermediate values on the Cl^- sweat test. Figure New approach for cystic fibrosis diagnosis based on skin-wipe sampling of forearm and analysis of ionic content (Cl^-/K^+ ratio) in skin-wipe extracts by capillary electrophoresis with contactless Conductometric Detection
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New approach for cystic fibrosis diagnosis based on chloride/potassium ratio analyzed in non-invasively obtained skin-wipe sweat samples by capillary electrophoresis with contactless Conductometric Detection.
Analytical and bioanalytical chemistry, 2017Co-Authors: Pavol Ďurč, František Foret, Eva Pokojová, Lukáš Homola, Jana Skřičková, Vladimír Herout, Milan Dastych, Hana Vinohradská, Petr KubáňAbstract:A new approach for sweat analysis used in cystic fibrosis (CF) diagnosis is proposed. It consists of a noninvasive skin-wipe sampling followed by analysis of target ions using capillary electrophoresis with contactless Conductometric Detection (C4D). The skin-wipe sampling consists of wiping a defined skin area with precleaned cotton swab moistened with 100 μL deionized water. The skin-wipe sample is then extracted for 3 min into 400 μL deionized water, and the extract is analyzed directly. The developed sampling method is cheap, simple, fast, and painless, and can replace the conventional pilocarpine-induced sweat chloride test commonly applied in CF diagnosis. The aqueous extract of the skin-wipe sample content is analyzed simultaneously by capillary electrophoresis with contactless Conductometric Detection using a double opposite end injection. A 20 mmol/L l-histidine/2-(N-morpholino)ethanesulfonic acid and 2 mmol/L 18-crown-6 at pH 6 electrolyte can separate all the major ions in less than 7 min. Skin-wipe sample extracts from 30 study participants—ten adult patients with CF (25–50 years old), ten pediatric patients with CF (1–15 years old), and ten healthy control individuals (1–18 years old)—were obtained and analyzed. From the analyzed ions in all samples, a significant difference between chloride and potassium concentrations was found in the CF patients and healthy controls. We propose the use of the Cl-/K+ ratio rather than the absolute Cl- concentration and a cutoff value of 4 in skin-wipe sample extracts as an alternative to the conventional sweat chloride analysis. The proposed Cl-/K+ ion ratio proved to be a more reliable indicator, is independent of the patient’s age, and allows better differentiation between non-CF individuals and CF patients having intermediate values on the Cl- sweat test.
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new approach for cystic fibrosis diagnosis based on chloride potassium ratio analyzed in non invasively obtained skin wipe sweat samples by capillary electrophoresis with contactless Conductometric Detection
Analytical and Bioanalytical Chemistry, 2017Co-Authors: Pavol ďurc, František Foret, Eva Pokojová, Lukáš Homola, Jana Skřičková, Vladimír Herout, Milan Dastych, Hana Vinohradská, Petr KubáňAbstract:A new approach for sweat analysis used in cystic fibrosis (CF) diagnosis is proposed. It consists of a noninvasive skin-wipe sampling followed by analysis of target ions using capillary electrophoresis with contactless Conductometric Detection (C4D). The skin-wipe sampling consists of wiping a defined skin area with precleaned cotton swab moistened with 100 μL deionized water. The skin-wipe sample is then extracted for 3 min into 400 μL deionized water, and the extract is analyzed directly. The developed sampling method is cheap, simple, fast, and painless, and can replace the conventional pilocarpine-induced sweat chloride test commonly applied in CF diagnosis. The aqueous extract of the skin-wipe sample content is analyzed simultaneously by capillary electrophoresis with contactless Conductometric Detection using a double opposite end injection. A 20 mmol/L l-histidine/2-(N-morpholino)ethanesulfonic acid and 2 mmol/L 18-crown-6 at pH 6 electrolyte can separate all the major ions in less than 7 min. Skin-wipe sample extracts from 30 study participants—ten adult patients with CF (25–50 years old), ten pediatric patients with CF (1–15 years old), and ten healthy control individuals (1–18 years old)—were obtained and analyzed. From the analyzed ions in all samples, a significant difference between chloride and potassium concentrations was found in the CF patients and healthy controls. We propose the use of the Cl-/K+ ratio rather than the absolute Cl- concentration and a cutoff value of 4 in skin-wipe sample extracts as an alternative to the conventional sweat chloride analysis. The proposed Cl-/K+ ion ratio proved to be a more reliable indicator, is independent of the patient’s age, and allows better differentiation between non-CF individuals and CF patients having intermediate values on the Cl- sweat test.
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Double opposite end injection capillary electrophoresis with contactless Conductometric Detection for simultaneous determination of chloride, sodium and potassium in cystic fibrosis diagnosis
Journal of chromatography. A, 2014Co-Authors: Petr Kubáň, Jana Skřičková, Eva Pokojová, Michal Greguš, František ForetAbstract:A novel approach for diagnosis of cystic fibrosis is presented. A simple and fast procedure to obtain sweat sample was developed. It consists of repeatedly wiping the skin of the forearm with deionized water moisturized cotton swab and extraction in 1mL of deionized water. Double opposite end injection capillary electrophoresis with contactless Conductometric Detection is used for the analysis of the extract. Chloride, sodium and potassium as the three target ions that participate in the ion transfer across the cellular membranes, and are affected by CF, are simultaneously determined in approximately 3min in a background electrolyte containing 20mM 2-(N-morpholino)ethanesulfonic acid, 20mM l-histidine and 2mM 18-crown-6. By using the target ion ratios rather than the concentrations of each individual ion combined with principal component analysis, the diagnosis of CF can be made more accurately and greatly reduce the number of false positive or negative results as is often the case when single ion (chloride) is analyzed.
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Separation of oxalate, formate and glycolate in human body fluid samples by capillary electrophoresis with contactless Conductometric Detection.
Journal of chromatography. A, 2013Co-Authors: Petr Kubáň, Pavol Ďurč, Miroslava Bittová, František ForetAbstract:A new method for rapid determination of toxic metabolites after methanol and ethylene glycol intoxication - oxalate, formate and glycolate in various body fluid samples (blood serum, saliva, urine, exhaled breath condensate) by capillary electrophoresis with contactless Conductometric Detection was developed. A selective separation of the three target analytes from other constituents present in the analyzed biological matrices was achieved in less than 6min in a fused silica capillary of 25μm I.D. using an electrolyte comprising 50mM l-histidine and 50mM 2-(N-morpholino)ethanesulfonic acid at pH 6.1. The only sample preparation was dilution with deionized water. The limits of Detection were 0.4, 0.6 and 1.3μM and limits of quantitation 1.3, 1.9 and 4.2μM for oxalate, formate and glycolate, respectively. The method provides a simple and rapid diagnostic test in suspected intoxication and is able to distinguish the ingested liquid, based on its metabolite trace. The method presents a fast screening tool that can be applicable in clinical practice.
František Foret - One of the best experts on this subject based on the ideXlab platform.
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New approach for cystic fibrosis diagnosis based on chloride/potassium ratio analyzed in non-invasively obtained skin-wipe sweat samples by capillary electrophoresis with contactless Conductometric Detection
Analytical and Bioanalytical Chemistry, 2017Co-Authors: Pavol Ďurč, František Foret, Eva Pokojová, Lukáš Homola, Jana Skřičková, Vladimír Herout, Milan Dastych, Hana Vinohradská, Petr KubáňAbstract:A new approach for sweat analysis used in cystic fibrosis (CF) diagnosis is proposed. It consists of a noninvasive skin-wipe sampling followed by analysis of target ions using capillary electrophoresis with contactless Conductometric Detection (C4D). The skin-wipe sampling consists of wiping a defined skin area with precleaned cotton swab moistened with 100 μL deionized water. The skin-wipe sample is then extracted for 3 min into 400 μL deionized water, and the extract is analyzed directly. The developed sampling method is cheap, simple, fast, and painless, and can replace the conventional pilocarpine-induced sweat chloride test commonly applied in CF diagnosis. The aqueous extract of the skin-wipe sample content is analyzed simultaneously by capillary electrophoresis with contactless Conductometric Detection using a double opposite end injection. A 20 mmol/L l -histidine/2-( N -morpholino)ethanesulfonic acid and 2 mmol/L 18-crown-6 at pH 6 electrolyte can separate all the major ions in less than 7 min. Skin-wipe sample extracts from 30 study participants—ten adult patients with CF (25–50 years old), ten pediatric patients with CF (1–15 years old), and ten healthy control individuals (1–18 years old)—were obtained and analyzed. From the analyzed ions in all samples, a significant difference between chloride and potassium concentrations was found in the CF patients and healthy controls. We propose the use of the Cl^-/K^+ ratio rather than the absolute Cl^- concentration and a cutoff value of 4 in skin-wipe sample extracts as an alternative to the conventional sweat chloride analysis. The proposed Cl^-/K^+ ion ratio proved to be a more reliable indicator, is independent of the patient’s age, and allows better differentiation between non-CF individuals and CF patients having intermediate values on the Cl^- sweat test. Figure New approach for cystic fibrosis diagnosis based on skin-wipe sampling of forearm and analysis of ionic content (Cl^-/K^+ ratio) in skin-wipe extracts by capillary electrophoresis with contactless Conductometric Detection
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New approach for cystic fibrosis diagnosis based on chloride/potassium ratio analyzed in non-invasively obtained skin-wipe sweat samples by capillary electrophoresis with contactless Conductometric Detection.
Analytical and bioanalytical chemistry, 2017Co-Authors: Pavol Ďurč, František Foret, Eva Pokojová, Lukáš Homola, Jana Skřičková, Vladimír Herout, Milan Dastych, Hana Vinohradská, Petr KubáňAbstract:A new approach for sweat analysis used in cystic fibrosis (CF) diagnosis is proposed. It consists of a noninvasive skin-wipe sampling followed by analysis of target ions using capillary electrophoresis with contactless Conductometric Detection (C4D). The skin-wipe sampling consists of wiping a defined skin area with precleaned cotton swab moistened with 100 μL deionized water. The skin-wipe sample is then extracted for 3 min into 400 μL deionized water, and the extract is analyzed directly. The developed sampling method is cheap, simple, fast, and painless, and can replace the conventional pilocarpine-induced sweat chloride test commonly applied in CF diagnosis. The aqueous extract of the skin-wipe sample content is analyzed simultaneously by capillary electrophoresis with contactless Conductometric Detection using a double opposite end injection. A 20 mmol/L l-histidine/2-(N-morpholino)ethanesulfonic acid and 2 mmol/L 18-crown-6 at pH 6 electrolyte can separate all the major ions in less than 7 min. Skin-wipe sample extracts from 30 study participants—ten adult patients with CF (25–50 years old), ten pediatric patients with CF (1–15 years old), and ten healthy control individuals (1–18 years old)—were obtained and analyzed. From the analyzed ions in all samples, a significant difference between chloride and potassium concentrations was found in the CF patients and healthy controls. We propose the use of the Cl-/K+ ratio rather than the absolute Cl- concentration and a cutoff value of 4 in skin-wipe sample extracts as an alternative to the conventional sweat chloride analysis. The proposed Cl-/K+ ion ratio proved to be a more reliable indicator, is independent of the patient’s age, and allows better differentiation between non-CF individuals and CF patients having intermediate values on the Cl- sweat test.
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new approach for cystic fibrosis diagnosis based on chloride potassium ratio analyzed in non invasively obtained skin wipe sweat samples by capillary electrophoresis with contactless Conductometric Detection
Analytical and Bioanalytical Chemistry, 2017Co-Authors: Pavol ďurc, František Foret, Eva Pokojová, Lukáš Homola, Jana Skřičková, Vladimír Herout, Milan Dastych, Hana Vinohradská, Petr KubáňAbstract:A new approach for sweat analysis used in cystic fibrosis (CF) diagnosis is proposed. It consists of a noninvasive skin-wipe sampling followed by analysis of target ions using capillary electrophoresis with contactless Conductometric Detection (C4D). The skin-wipe sampling consists of wiping a defined skin area with precleaned cotton swab moistened with 100 μL deionized water. The skin-wipe sample is then extracted for 3 min into 400 μL deionized water, and the extract is analyzed directly. The developed sampling method is cheap, simple, fast, and painless, and can replace the conventional pilocarpine-induced sweat chloride test commonly applied in CF diagnosis. The aqueous extract of the skin-wipe sample content is analyzed simultaneously by capillary electrophoresis with contactless Conductometric Detection using a double opposite end injection. A 20 mmol/L l-histidine/2-(N-morpholino)ethanesulfonic acid and 2 mmol/L 18-crown-6 at pH 6 electrolyte can separate all the major ions in less than 7 min. Skin-wipe sample extracts from 30 study participants—ten adult patients with CF (25–50 years old), ten pediatric patients with CF (1–15 years old), and ten healthy control individuals (1–18 years old)—were obtained and analyzed. From the analyzed ions in all samples, a significant difference between chloride and potassium concentrations was found in the CF patients and healthy controls. We propose the use of the Cl-/K+ ratio rather than the absolute Cl- concentration and a cutoff value of 4 in skin-wipe sample extracts as an alternative to the conventional sweat chloride analysis. The proposed Cl-/K+ ion ratio proved to be a more reliable indicator, is independent of the patient’s age, and allows better differentiation between non-CF individuals and CF patients having intermediate values on the Cl- sweat test.
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Double opposite end injection capillary electrophoresis with contactless Conductometric Detection for simultaneous determination of chloride, sodium and potassium in cystic fibrosis diagnosis
Journal of chromatography. A, 2014Co-Authors: Petr Kubáň, Jana Skřičková, Eva Pokojová, Michal Greguš, František ForetAbstract:A novel approach for diagnosis of cystic fibrosis is presented. A simple and fast procedure to obtain sweat sample was developed. It consists of repeatedly wiping the skin of the forearm with deionized water moisturized cotton swab and extraction in 1mL of deionized water. Double opposite end injection capillary electrophoresis with contactless Conductometric Detection is used for the analysis of the extract. Chloride, sodium and potassium as the three target ions that participate in the ion transfer across the cellular membranes, and are affected by CF, are simultaneously determined in approximately 3min in a background electrolyte containing 20mM 2-(N-morpholino)ethanesulfonic acid, 20mM l-histidine and 2mM 18-crown-6. By using the target ion ratios rather than the concentrations of each individual ion combined with principal component analysis, the diagnosis of CF can be made more accurately and greatly reduce the number of false positive or negative results as is often the case when single ion (chloride) is analyzed.
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Separation of oxalate, formate and glycolate in human body fluid samples by capillary electrophoresis with contactless Conductometric Detection.
Journal of chromatography. A, 2013Co-Authors: Petr Kubáň, Pavol Ďurč, Miroslava Bittová, František ForetAbstract:A new method for rapid determination of toxic metabolites after methanol and ethylene glycol intoxication - oxalate, formate and glycolate in various body fluid samples (blood serum, saliva, urine, exhaled breath condensate) by capillary electrophoresis with contactless Conductometric Detection was developed. A selective separation of the three target analytes from other constituents present in the analyzed biological matrices was achieved in less than 6min in a fused silica capillary of 25μm I.D. using an electrolyte comprising 50mM l-histidine and 50mM 2-(N-morpholino)ethanesulfonic acid at pH 6.1. The only sample preparation was dilution with deionized water. The limits of Detection were 0.4, 0.6 and 1.3μM and limits of quantitation 1.3, 1.9 and 4.2μM for oxalate, formate and glycolate, respectively. The method provides a simple and rapid diagnostic test in suspected intoxication and is able to distinguish the ingested liquid, based on its metabolite trace. The method presents a fast screening tool that can be applicable in clinical practice.
Peter D. Carpenter - One of the best experts on this subject based on the ideXlab platform.
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Enhanced Conductometric Detection of cyanide in suppressed ion chromatography.
Journal of Chromatography A, 2000Co-Authors: Arety Caliamanis, Malcolm J. Mccormick, Peter D. CarpenterAbstract:Abstract Weak-acid anions such as borate and cyanide, present problems in chemically suppressed ion chromatography, since the acids are weakly ionised, giving low conductivity and therefore decreased sensitivity. For borate this problem was overcome by converting the weak acid to its sodium salt, by the use of a second anion micromembrane suppressor (AMMS) as an ion exchange reactor (IER), flushed by EDTA reagent, to supply sodium ions for the conversion. This paper will discuss the use of this IER system to determine cyanide, which is also a very weak acid, with a similar pKa to borate. The Detection limit for cyanide of 50 μM compare favourably with indirect conductivity Detection.
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Enhancement of Conductometric Detection of weak acids in ion chromatography.
Journal of Chromatography A, 1999Co-Authors: Arety Caliamanis, Malcolm J. Mccormick, Peter D. CarpenterAbstract:Anions of weak acids can represent a problem when determined via chemically suppressed ion chromatography as the acids can be weakly ionised, giving low conductivity and hence low sensitivity. Previous work showed that converting some weak acids back to their sodium salts, using a second micromembrane suppressor, greatly enhanced conductivity and thus sensitivity. This paper will discuss further work in optimising the conversion for boric acid by using sodium salts of EDTA and the mechanisms involved.
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Conductometric Detection of anions of weak acids in chemically suppressed ion chromatography
Analytical Chemistry, 1997Co-Authors: Arety Caliamanis, And Malcolm J. Mccormick, Peter D. CarpenterAbstract:A commercial micromembrane suppressor, usually used to chemically suppress eluent conductance in ion chromatography, has been successfully used to effect ion replacement reactions in suppressed eluent streams. For 10 mM fluoride and 100 μM acetate, there were net decreases in conductance upon conversion of the acids to the sodium salts, showing that these concentrations were below their critical point concentrations (CPCs), defined here as the formal concentration above which a specified conjugate salt has a higher conductance than the same formal concentration of the weak acid. For 10 mM carbonate and 10 μM borate, there were net increases in conductance, showing that these concentrations were above their respective CPCs. The most effective of several ion replacement reagents investigated was EDTA at pH 11, which, for 5.0 mM borate, produced 250- and 1400-fold increases in peak height and peak area, respectively, compared with a normal IC system and low background conductance. The applicability of the sy...
Eva Pokojová - One of the best experts on this subject based on the ideXlab platform.
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New approach for cystic fibrosis diagnosis based on chloride/potassium ratio analyzed in non-invasively obtained skin-wipe sweat samples by capillary electrophoresis with contactless Conductometric Detection
Analytical and Bioanalytical Chemistry, 2017Co-Authors: Pavol Ďurč, František Foret, Eva Pokojová, Lukáš Homola, Jana Skřičková, Vladimír Herout, Milan Dastych, Hana Vinohradská, Petr KubáňAbstract:A new approach for sweat analysis used in cystic fibrosis (CF) diagnosis is proposed. It consists of a noninvasive skin-wipe sampling followed by analysis of target ions using capillary electrophoresis with contactless Conductometric Detection (C4D). The skin-wipe sampling consists of wiping a defined skin area with precleaned cotton swab moistened with 100 μL deionized water. The skin-wipe sample is then extracted for 3 min into 400 μL deionized water, and the extract is analyzed directly. The developed sampling method is cheap, simple, fast, and painless, and can replace the conventional pilocarpine-induced sweat chloride test commonly applied in CF diagnosis. The aqueous extract of the skin-wipe sample content is analyzed simultaneously by capillary electrophoresis with contactless Conductometric Detection using a double opposite end injection. A 20 mmol/L l -histidine/2-( N -morpholino)ethanesulfonic acid and 2 mmol/L 18-crown-6 at pH 6 electrolyte can separate all the major ions in less than 7 min. Skin-wipe sample extracts from 30 study participants—ten adult patients with CF (25–50 years old), ten pediatric patients with CF (1–15 years old), and ten healthy control individuals (1–18 years old)—were obtained and analyzed. From the analyzed ions in all samples, a significant difference between chloride and potassium concentrations was found in the CF patients and healthy controls. We propose the use of the Cl^-/K^+ ratio rather than the absolute Cl^- concentration and a cutoff value of 4 in skin-wipe sample extracts as an alternative to the conventional sweat chloride analysis. The proposed Cl^-/K^+ ion ratio proved to be a more reliable indicator, is independent of the patient’s age, and allows better differentiation between non-CF individuals and CF patients having intermediate values on the Cl^- sweat test. Figure New approach for cystic fibrosis diagnosis based on skin-wipe sampling of forearm and analysis of ionic content (Cl^-/K^+ ratio) in skin-wipe extracts by capillary electrophoresis with contactless Conductometric Detection
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New approach for cystic fibrosis diagnosis based on chloride/potassium ratio analyzed in non-invasively obtained skin-wipe sweat samples by capillary electrophoresis with contactless Conductometric Detection.
Analytical and bioanalytical chemistry, 2017Co-Authors: Pavol Ďurč, František Foret, Eva Pokojová, Lukáš Homola, Jana Skřičková, Vladimír Herout, Milan Dastych, Hana Vinohradská, Petr KubáňAbstract:A new approach for sweat analysis used in cystic fibrosis (CF) diagnosis is proposed. It consists of a noninvasive skin-wipe sampling followed by analysis of target ions using capillary electrophoresis with contactless Conductometric Detection (C4D). The skin-wipe sampling consists of wiping a defined skin area with precleaned cotton swab moistened with 100 μL deionized water. The skin-wipe sample is then extracted for 3 min into 400 μL deionized water, and the extract is analyzed directly. The developed sampling method is cheap, simple, fast, and painless, and can replace the conventional pilocarpine-induced sweat chloride test commonly applied in CF diagnosis. The aqueous extract of the skin-wipe sample content is analyzed simultaneously by capillary electrophoresis with contactless Conductometric Detection using a double opposite end injection. A 20 mmol/L l-histidine/2-(N-morpholino)ethanesulfonic acid and 2 mmol/L 18-crown-6 at pH 6 electrolyte can separate all the major ions in less than 7 min. Skin-wipe sample extracts from 30 study participants—ten adult patients with CF (25–50 years old), ten pediatric patients with CF (1–15 years old), and ten healthy control individuals (1–18 years old)—were obtained and analyzed. From the analyzed ions in all samples, a significant difference between chloride and potassium concentrations was found in the CF patients and healthy controls. We propose the use of the Cl-/K+ ratio rather than the absolute Cl- concentration and a cutoff value of 4 in skin-wipe sample extracts as an alternative to the conventional sweat chloride analysis. The proposed Cl-/K+ ion ratio proved to be a more reliable indicator, is independent of the patient’s age, and allows better differentiation between non-CF individuals and CF patients having intermediate values on the Cl- sweat test.
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new approach for cystic fibrosis diagnosis based on chloride potassium ratio analyzed in non invasively obtained skin wipe sweat samples by capillary electrophoresis with contactless Conductometric Detection
Analytical and Bioanalytical Chemistry, 2017Co-Authors: Pavol ďurc, František Foret, Eva Pokojová, Lukáš Homola, Jana Skřičková, Vladimír Herout, Milan Dastych, Hana Vinohradská, Petr KubáňAbstract:A new approach for sweat analysis used in cystic fibrosis (CF) diagnosis is proposed. It consists of a noninvasive skin-wipe sampling followed by analysis of target ions using capillary electrophoresis with contactless Conductometric Detection (C4D). The skin-wipe sampling consists of wiping a defined skin area with precleaned cotton swab moistened with 100 μL deionized water. The skin-wipe sample is then extracted for 3 min into 400 μL deionized water, and the extract is analyzed directly. The developed sampling method is cheap, simple, fast, and painless, and can replace the conventional pilocarpine-induced sweat chloride test commonly applied in CF diagnosis. The aqueous extract of the skin-wipe sample content is analyzed simultaneously by capillary electrophoresis with contactless Conductometric Detection using a double opposite end injection. A 20 mmol/L l-histidine/2-(N-morpholino)ethanesulfonic acid and 2 mmol/L 18-crown-6 at pH 6 electrolyte can separate all the major ions in less than 7 min. Skin-wipe sample extracts from 30 study participants—ten adult patients with CF (25–50 years old), ten pediatric patients with CF (1–15 years old), and ten healthy control individuals (1–18 years old)—were obtained and analyzed. From the analyzed ions in all samples, a significant difference between chloride and potassium concentrations was found in the CF patients and healthy controls. We propose the use of the Cl-/K+ ratio rather than the absolute Cl- concentration and a cutoff value of 4 in skin-wipe sample extracts as an alternative to the conventional sweat chloride analysis. The proposed Cl-/K+ ion ratio proved to be a more reliable indicator, is independent of the patient’s age, and allows better differentiation between non-CF individuals and CF patients having intermediate values on the Cl- sweat test.
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Double opposite end injection capillary electrophoresis with contactless Conductometric Detection for simultaneous determination of chloride, sodium and potassium in cystic fibrosis diagnosis
Journal of chromatography. A, 2014Co-Authors: Petr Kubáň, Jana Skřičková, Eva Pokojová, Michal Greguš, František ForetAbstract:A novel approach for diagnosis of cystic fibrosis is presented. A simple and fast procedure to obtain sweat sample was developed. It consists of repeatedly wiping the skin of the forearm with deionized water moisturized cotton swab and extraction in 1mL of deionized water. Double opposite end injection capillary electrophoresis with contactless Conductometric Detection is used for the analysis of the extract. Chloride, sodium and potassium as the three target ions that participate in the ion transfer across the cellular membranes, and are affected by CF, are simultaneously determined in approximately 3min in a background electrolyte containing 20mM 2-(N-morpholino)ethanesulfonic acid, 20mM l-histidine and 2mM 18-crown-6. By using the target ion ratios rather than the concentrations of each individual ion combined with principal component analysis, the diagnosis of CF can be made more accurately and greatly reduce the number of false positive or negative results as is often the case when single ion (chloride) is analyzed.
Jana Skřičková - One of the best experts on this subject based on the ideXlab platform.
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New approach for cystic fibrosis diagnosis based on chloride/potassium ratio analyzed in non-invasively obtained skin-wipe sweat samples by capillary electrophoresis with contactless Conductometric Detection
Analytical and Bioanalytical Chemistry, 2017Co-Authors: Pavol Ďurč, František Foret, Eva Pokojová, Lukáš Homola, Jana Skřičková, Vladimír Herout, Milan Dastych, Hana Vinohradská, Petr KubáňAbstract:A new approach for sweat analysis used in cystic fibrosis (CF) diagnosis is proposed. It consists of a noninvasive skin-wipe sampling followed by analysis of target ions using capillary electrophoresis with contactless Conductometric Detection (C4D). The skin-wipe sampling consists of wiping a defined skin area with precleaned cotton swab moistened with 100 μL deionized water. The skin-wipe sample is then extracted for 3 min into 400 μL deionized water, and the extract is analyzed directly. The developed sampling method is cheap, simple, fast, and painless, and can replace the conventional pilocarpine-induced sweat chloride test commonly applied in CF diagnosis. The aqueous extract of the skin-wipe sample content is analyzed simultaneously by capillary electrophoresis with contactless Conductometric Detection using a double opposite end injection. A 20 mmol/L l -histidine/2-( N -morpholino)ethanesulfonic acid and 2 mmol/L 18-crown-6 at pH 6 electrolyte can separate all the major ions in less than 7 min. Skin-wipe sample extracts from 30 study participants—ten adult patients with CF (25–50 years old), ten pediatric patients with CF (1–15 years old), and ten healthy control individuals (1–18 years old)—were obtained and analyzed. From the analyzed ions in all samples, a significant difference between chloride and potassium concentrations was found in the CF patients and healthy controls. We propose the use of the Cl^-/K^+ ratio rather than the absolute Cl^- concentration and a cutoff value of 4 in skin-wipe sample extracts as an alternative to the conventional sweat chloride analysis. The proposed Cl^-/K^+ ion ratio proved to be a more reliable indicator, is independent of the patient’s age, and allows better differentiation between non-CF individuals and CF patients having intermediate values on the Cl^- sweat test. Figure New approach for cystic fibrosis diagnosis based on skin-wipe sampling of forearm and analysis of ionic content (Cl^-/K^+ ratio) in skin-wipe extracts by capillary electrophoresis with contactless Conductometric Detection
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New approach for cystic fibrosis diagnosis based on chloride/potassium ratio analyzed in non-invasively obtained skin-wipe sweat samples by capillary electrophoresis with contactless Conductometric Detection.
Analytical and bioanalytical chemistry, 2017Co-Authors: Pavol Ďurč, František Foret, Eva Pokojová, Lukáš Homola, Jana Skřičková, Vladimír Herout, Milan Dastych, Hana Vinohradská, Petr KubáňAbstract:A new approach for sweat analysis used in cystic fibrosis (CF) diagnosis is proposed. It consists of a noninvasive skin-wipe sampling followed by analysis of target ions using capillary electrophoresis with contactless Conductometric Detection (C4D). The skin-wipe sampling consists of wiping a defined skin area with precleaned cotton swab moistened with 100 μL deionized water. The skin-wipe sample is then extracted for 3 min into 400 μL deionized water, and the extract is analyzed directly. The developed sampling method is cheap, simple, fast, and painless, and can replace the conventional pilocarpine-induced sweat chloride test commonly applied in CF diagnosis. The aqueous extract of the skin-wipe sample content is analyzed simultaneously by capillary electrophoresis with contactless Conductometric Detection using a double opposite end injection. A 20 mmol/L l-histidine/2-(N-morpholino)ethanesulfonic acid and 2 mmol/L 18-crown-6 at pH 6 electrolyte can separate all the major ions in less than 7 min. Skin-wipe sample extracts from 30 study participants—ten adult patients with CF (25–50 years old), ten pediatric patients with CF (1–15 years old), and ten healthy control individuals (1–18 years old)—were obtained and analyzed. From the analyzed ions in all samples, a significant difference between chloride and potassium concentrations was found in the CF patients and healthy controls. We propose the use of the Cl-/K+ ratio rather than the absolute Cl- concentration and a cutoff value of 4 in skin-wipe sample extracts as an alternative to the conventional sweat chloride analysis. The proposed Cl-/K+ ion ratio proved to be a more reliable indicator, is independent of the patient’s age, and allows better differentiation between non-CF individuals and CF patients having intermediate values on the Cl- sweat test.
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new approach for cystic fibrosis diagnosis based on chloride potassium ratio analyzed in non invasively obtained skin wipe sweat samples by capillary electrophoresis with contactless Conductometric Detection
Analytical and Bioanalytical Chemistry, 2017Co-Authors: Pavol ďurc, František Foret, Eva Pokojová, Lukáš Homola, Jana Skřičková, Vladimír Herout, Milan Dastych, Hana Vinohradská, Petr KubáňAbstract:A new approach for sweat analysis used in cystic fibrosis (CF) diagnosis is proposed. It consists of a noninvasive skin-wipe sampling followed by analysis of target ions using capillary electrophoresis with contactless Conductometric Detection (C4D). The skin-wipe sampling consists of wiping a defined skin area with precleaned cotton swab moistened with 100 μL deionized water. The skin-wipe sample is then extracted for 3 min into 400 μL deionized water, and the extract is analyzed directly. The developed sampling method is cheap, simple, fast, and painless, and can replace the conventional pilocarpine-induced sweat chloride test commonly applied in CF diagnosis. The aqueous extract of the skin-wipe sample content is analyzed simultaneously by capillary electrophoresis with contactless Conductometric Detection using a double opposite end injection. A 20 mmol/L l-histidine/2-(N-morpholino)ethanesulfonic acid and 2 mmol/L 18-crown-6 at pH 6 electrolyte can separate all the major ions in less than 7 min. Skin-wipe sample extracts from 30 study participants—ten adult patients with CF (25–50 years old), ten pediatric patients with CF (1–15 years old), and ten healthy control individuals (1–18 years old)—were obtained and analyzed. From the analyzed ions in all samples, a significant difference between chloride and potassium concentrations was found in the CF patients and healthy controls. We propose the use of the Cl-/K+ ratio rather than the absolute Cl- concentration and a cutoff value of 4 in skin-wipe sample extracts as an alternative to the conventional sweat chloride analysis. The proposed Cl-/K+ ion ratio proved to be a more reliable indicator, is independent of the patient’s age, and allows better differentiation between non-CF individuals and CF patients having intermediate values on the Cl- sweat test.
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Double opposite end injection capillary electrophoresis with contactless Conductometric Detection for simultaneous determination of chloride, sodium and potassium in cystic fibrosis diagnosis
Journal of chromatography. A, 2014Co-Authors: Petr Kubáň, Jana Skřičková, Eva Pokojová, Michal Greguš, František ForetAbstract:A novel approach for diagnosis of cystic fibrosis is presented. A simple and fast procedure to obtain sweat sample was developed. It consists of repeatedly wiping the skin of the forearm with deionized water moisturized cotton swab and extraction in 1mL of deionized water. Double opposite end injection capillary electrophoresis with contactless Conductometric Detection is used for the analysis of the extract. Chloride, sodium and potassium as the three target ions that participate in the ion transfer across the cellular membranes, and are affected by CF, are simultaneously determined in approximately 3min in a background electrolyte containing 20mM 2-(N-morpholino)ethanesulfonic acid, 20mM l-histidine and 2mM 18-crown-6. By using the target ion ratios rather than the concentrations of each individual ion combined with principal component analysis, the diagnosis of CF can be made more accurately and greatly reduce the number of false positive or negative results as is often the case when single ion (chloride) is analyzed.