The Experts below are selected from a list of 152112 Experts worldwide ranked by ideXlab platform

Urs Von Stockar - One of the best experts on this subject based on the ideXlab platform.

  • on line biomass monitoring of cho perfusion culture with scanning dielectric spectroscopy
    Biotechnology and Bioengineering, 2003
    Co-Authors: Christopher Cannizzaro, Raphael Gugerli, I W Marison, Urs Von Stockar
    Abstract:

    In this work, dielec. spectroscopy was used to monitor two CHO perfusion culture expts. (B14 and B16). The capacitance of the cell suspension was recorded every 20 min over an excitation frequency range of 0.2 MHz to 10.0 MHz. A phase plot of the capacitance at a low excitation frequency vs. the value at a higher frequency proved to be an accurate indicator of the major transition Points of the culture, i.e., max. cell viability, end of lactate Consumption, Point of zero viability. For both expts., the capacitance signal correlated very well (R2 >0.98) with viable cell no. up to concns. of 1 * 107 cells/mL. Visual observation of the capacitance spectra indicated that changes in the capacitance relative to frequency were related to the cellular morphol. A multivariate model was developed using off-line data that could predict the median cell diam. within a single expt. (B14) with an error of 0.34 mm (2%). Upon extension to a subsequent expt. (B16), the predicted error was 1.18 mm (9%). [on SciFinder (R)]

  • on line biomass monitoring of cho perfusion culture with scanning dielectric spectroscopy
    Biotechnology and Bioengineering, 2003
    Co-Authors: Christopher Cannizzaro, Raphael Gugerli, I W Marison, Urs Von Stockar
    Abstract:

    In this work, dielectric spectroscopy was used to monitor two CHO perfusion culture experiments (B14 and B16). The capacitance of the cell suspension was recorded every 20 minutes over an excitation frequency range of 0.2 MHz to 10.0 MHz. A phase plot of the capacitance at a low excitation frequency vs. the value at a higher frequency proved to be an accurate indicator of the major transition Points of the culture, i.e., maximum cell viability, end of lactate Consumption, Point of zero viability. For both experiments, the capacitance signal correlated very well (R(2) >0.98) with viable cell number up to concentrations of 1 x 10(7) cells/mL. Visual observation of the capacitance spectra indicated that changes in the capacitance relative to frequency were related to the cellular morphology. A multivariate model was developed using off-line data that could predict the median cell diameter within a single experiment (B14) with an error of 0.34 microm (2%). Upon extension to a subsequent experiment (B16), the predicted error was 1.18 microm (9%).

Christopher Cannizzaro - One of the best experts on this subject based on the ideXlab platform.

  • on line biomass monitoring of cho perfusion culture with scanning dielectric spectroscopy
    Biotechnology and Bioengineering, 2003
    Co-Authors: Christopher Cannizzaro, Raphael Gugerli, I W Marison, Urs Von Stockar
    Abstract:

    In this work, dielec. spectroscopy was used to monitor two CHO perfusion culture expts. (B14 and B16). The capacitance of the cell suspension was recorded every 20 min over an excitation frequency range of 0.2 MHz to 10.0 MHz. A phase plot of the capacitance at a low excitation frequency vs. the value at a higher frequency proved to be an accurate indicator of the major transition Points of the culture, i.e., max. cell viability, end of lactate Consumption, Point of zero viability. For both expts., the capacitance signal correlated very well (R2 >0.98) with viable cell no. up to concns. of 1 * 107 cells/mL. Visual observation of the capacitance spectra indicated that changes in the capacitance relative to frequency were related to the cellular morphol. A multivariate model was developed using off-line data that could predict the median cell diam. within a single expt. (B14) with an error of 0.34 mm (2%). Upon extension to a subsequent expt. (B16), the predicted error was 1.18 mm (9%). [on SciFinder (R)]

  • on line biomass monitoring of cho perfusion culture with scanning dielectric spectroscopy
    Biotechnology and Bioengineering, 2003
    Co-Authors: Christopher Cannizzaro, Raphael Gugerli, I W Marison, Urs Von Stockar
    Abstract:

    In this work, dielectric spectroscopy was used to monitor two CHO perfusion culture experiments (B14 and B16). The capacitance of the cell suspension was recorded every 20 minutes over an excitation frequency range of 0.2 MHz to 10.0 MHz. A phase plot of the capacitance at a low excitation frequency vs. the value at a higher frequency proved to be an accurate indicator of the major transition Points of the culture, i.e., maximum cell viability, end of lactate Consumption, Point of zero viability. For both experiments, the capacitance signal correlated very well (R(2) >0.98) with viable cell number up to concentrations of 1 x 10(7) cells/mL. Visual observation of the capacitance spectra indicated that changes in the capacitance relative to frequency were related to the cellular morphology. A multivariate model was developed using off-line data that could predict the median cell diameter within a single experiment (B14) with an error of 0.34 microm (2%). Upon extension to a subsequent experiment (B16), the predicted error was 1.18 microm (9%).

I W Marison - One of the best experts on this subject based on the ideXlab platform.

  • on line biomass monitoring of cho perfusion culture with scanning dielectric spectroscopy
    Biotechnology and Bioengineering, 2003
    Co-Authors: Christopher Cannizzaro, Raphael Gugerli, I W Marison, Urs Von Stockar
    Abstract:

    In this work, dielec. spectroscopy was used to monitor two CHO perfusion culture expts. (B14 and B16). The capacitance of the cell suspension was recorded every 20 min over an excitation frequency range of 0.2 MHz to 10.0 MHz. A phase plot of the capacitance at a low excitation frequency vs. the value at a higher frequency proved to be an accurate indicator of the major transition Points of the culture, i.e., max. cell viability, end of lactate Consumption, Point of zero viability. For both expts., the capacitance signal correlated very well (R2 >0.98) with viable cell no. up to concns. of 1 * 107 cells/mL. Visual observation of the capacitance spectra indicated that changes in the capacitance relative to frequency were related to the cellular morphol. A multivariate model was developed using off-line data that could predict the median cell diam. within a single expt. (B14) with an error of 0.34 mm (2%). Upon extension to a subsequent expt. (B16), the predicted error was 1.18 mm (9%). [on SciFinder (R)]

  • on line biomass monitoring of cho perfusion culture with scanning dielectric spectroscopy
    Biotechnology and Bioengineering, 2003
    Co-Authors: Christopher Cannizzaro, Raphael Gugerli, I W Marison, Urs Von Stockar
    Abstract:

    In this work, dielectric spectroscopy was used to monitor two CHO perfusion culture experiments (B14 and B16). The capacitance of the cell suspension was recorded every 20 minutes over an excitation frequency range of 0.2 MHz to 10.0 MHz. A phase plot of the capacitance at a low excitation frequency vs. the value at a higher frequency proved to be an accurate indicator of the major transition Points of the culture, i.e., maximum cell viability, end of lactate Consumption, Point of zero viability. For both experiments, the capacitance signal correlated very well (R(2) >0.98) with viable cell number up to concentrations of 1 x 10(7) cells/mL. Visual observation of the capacitance spectra indicated that changes in the capacitance relative to frequency were related to the cellular morphology. A multivariate model was developed using off-line data that could predict the median cell diameter within a single experiment (B14) with an error of 0.34 microm (2%). Upon extension to a subsequent experiment (B16), the predicted error was 1.18 microm (9%).

Raphael Gugerli - One of the best experts on this subject based on the ideXlab platform.

  • on line biomass monitoring of cho perfusion culture with scanning dielectric spectroscopy
    Biotechnology and Bioengineering, 2003
    Co-Authors: Christopher Cannizzaro, Raphael Gugerli, I W Marison, Urs Von Stockar
    Abstract:

    In this work, dielec. spectroscopy was used to monitor two CHO perfusion culture expts. (B14 and B16). The capacitance of the cell suspension was recorded every 20 min over an excitation frequency range of 0.2 MHz to 10.0 MHz. A phase plot of the capacitance at a low excitation frequency vs. the value at a higher frequency proved to be an accurate indicator of the major transition Points of the culture, i.e., max. cell viability, end of lactate Consumption, Point of zero viability. For both expts., the capacitance signal correlated very well (R2 >0.98) with viable cell no. up to concns. of 1 * 107 cells/mL. Visual observation of the capacitance spectra indicated that changes in the capacitance relative to frequency were related to the cellular morphol. A multivariate model was developed using off-line data that could predict the median cell diam. within a single expt. (B14) with an error of 0.34 mm (2%). Upon extension to a subsequent expt. (B16), the predicted error was 1.18 mm (9%). [on SciFinder (R)]

  • on line biomass monitoring of cho perfusion culture with scanning dielectric spectroscopy
    Biotechnology and Bioengineering, 2003
    Co-Authors: Christopher Cannizzaro, Raphael Gugerli, I W Marison, Urs Von Stockar
    Abstract:

    In this work, dielectric spectroscopy was used to monitor two CHO perfusion culture experiments (B14 and B16). The capacitance of the cell suspension was recorded every 20 minutes over an excitation frequency range of 0.2 MHz to 10.0 MHz. A phase plot of the capacitance at a low excitation frequency vs. the value at a higher frequency proved to be an accurate indicator of the major transition Points of the culture, i.e., maximum cell viability, end of lactate Consumption, Point of zero viability. For both experiments, the capacitance signal correlated very well (R(2) >0.98) with viable cell number up to concentrations of 1 x 10(7) cells/mL. Visual observation of the capacitance spectra indicated that changes in the capacitance relative to frequency were related to the cellular morphology. A multivariate model was developed using off-line data that could predict the median cell diameter within a single experiment (B14) with an error of 0.34 microm (2%). Upon extension to a subsequent experiment (B16), the predicted error was 1.18 microm (9%).

Massoud Tabesh - One of the best experts on this subject based on the ideXlab platform.

  • Integrated risk assessment of urban water supply systems from source to tap
    Stochastic Environmental Research and Risk Assessment, 2013
    Co-Authors: Abbas Roozbahani, Banafsheh Zahraie, Massoud Tabesh
    Abstract:

    Urban water supply systems (UWSS) are generally composed of water sources, transmission pipes, treatment plants, and distribution networks from source to tap and usually are exposed to variety of uncertain threatening hazards. These threats can be divided to three main groups of natural, human-made, and operational hazards which affect either water quantity or water quality. In order to evaluate the reliability of water supply systems, risk assessment tools must be used to identify threats, their probability, and consequences and vulnerabilities of each element of these systems against the hazards. Due to the complexity and uncertainties affecting water supply systems and threatening hazards, a comprehensive and effective risk assessment method is required. In this study, an integrated fuzzy hierarchical risk assessment model for water supply systems (IFHRA-WSS) is proposed to assess hazards in a complex UWSS using a systematic approach incorporating both water quantity and quality issues. This model uses a hierarchical framework for breaking down the UWSS infrastructures to their interrelated elements to reduce the overall complexity of the system. It also considers uncertainties using Fuzzy Logic approach. Effects of functional interdependencies between different components of the system have also been considered in the vulnerability analysis. IFHRA-WSS incorporates the contributions of urban water experts in a group risk assessment procedure in a way that they can be easily expressed in terms of the qualitative and quantitative risk measures. Efficiency of this model has been examined in a case study which includes a large part of a drinking water supply system in a major city in Iran. This system includes all the elements of the UWSS from the delivery Point to the Consumption Point. In the case study, different components and subcomponents of this system have been ranked based on their estimated risk values. It is envisaged that the results of the proposed model can help the decision makers to plan for effective risk mitigation measures.