The Experts below are selected from a list of 54 Experts worldwide ranked by ideXlab platform
Atul N. Parikh - One of the best experts on this subject based on the ideXlab platform.
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Lithographically Defined Macroscale Modulation of Lateral Fluidity and Phase Separation Realized via Patterned Nanoporous Silica-Supported Phospholipid Bilayers
2016Co-Authors: Eric L. Kendall, Viviane N. Ngassam, Sean F. Gilmore, Jeffrey C. Brinker, Atul N. ParikhAbstract:Using lithographically defined surfaces consisting of hydrophilic patterns of nanoporous and nonporous (bulk) amorphous silica, we show that fusion of small, unilamellar lipid vesicles produces a single, Contiguous, Fluid bilayer phase experiencing a predetermined pattern of interfacial interactions. Although long-range lateral Fluidity of the bilayer, characterized by fluorescence recovery after photobleaching, indicates a nominally single average diffusion constant, fluorescence microscopy-based measurements of temperature-dependent onset of Fluidity reveals a locally enhanced Fluidity for bilayer regions supported on nanoporous silica in the vicinity of the Fluid–gel transition temperature. Furthermore, thermally quenching lipid bilayers composed of a binary lipid mixture below its apparent miscibility transition temperature induces qualitatively different lateral phase separation in each region of the supported bilayer: The nanoporous substrate produces large, microscopic domains (and domain-aggregates), whereas surface texture characterized by much smaller domains and devoid of any domain-aggregates appears on bulk glass-supported regions of the single-lipid bilayer. Interestingly, lateral distribution of the constituent molecules also reveals an enrichment of gel-phase lipids over nanoporous regions, presumably as a consequence of differential mobilities of constituent lipids across the topographic bulk/nanoporous boundary. Together, these results reveal that subtle local variations in constraints imposed at the bilayer interface, such as by spatial variations in roughness and substrate adhesion, can give rise to significant differences in macroscale biophysical properties of phospholipid bilayers even within a single, Contiguous phase
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Lithographically Defined Macroscale Modulation of Lateral Fluidity and Phase Separation Realized via Patterned Nanoporous Silica-Supported Phospholipid Bilayers
Journal of the American Chemical Society, 2013Co-Authors: Eric L. Kendall, Viviane N. Ngassam, Sean F. Gilmore, C. Jeffrey Brinker, Atul N. ParikhAbstract:Using lithographically defined surfaces consisting of hydrophilic patterns of nanoporous and nonporous (bulk) amorphous silica, we show that fusion of small, unilamellar lipid vesicles produces a single, Contiguous, Fluid bilayer phase experiencing a predetermined pattern of interfacial interactions. Although long-range lateral Fluidity of the bilayer, characterized by fluorescence recovery after photobleaching, indicates a nominally single average diffusion constant, fluorescence microscopy-based measurements of temperature-dependent onset of Fluidity reveals a locally enhanced Fluidity for bilayer regions supported on nanoporous silica in the vicinity of the Fluid–gel transition temperature. Furthermore, thermally quenching lipid bilayers composed of a binary lipid mixture below its apparent miscibility transition temperature induces qualitatively different lateral phase separation in each region of the supported bilayer: The nanoporous substrate produces large, microscopic domains (and domain-aggregate...
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Characterization of Supported Membranes on Topographically Patterned Polymeric Elastomers and Their Applications to Microcontact Printing
Langmuir : the ACS journal of surfaces and colloids, 2007Co-Authors: Annapoorna R. Sapuri-butti, Ravi Chandra Butti, Atul N. ParikhAbstract:This article describes the fluorescence microscopy and imaging ellipsometry-based characterization of supported phospholipid bilayer formation on elastomeric substrates and its application in microcontact printing of spatially patterned phospholipid bilayers. Elastomeric stamps, displaying a uniformly spaced array of square wells (20, 50, and 100 μm linear dimensions), are prepared using poly(dimethyl)siloxane from photolithographically derived silicon masters. Exposing elastomeric stamps, following UV/ozone-induced oxidation, to a solution of small unilamellar phospholipid vesicles results in the formation of a 2D Contiguous, Fluid phospholipid bilayers. The bilayer covers both the elevated and depressed regions of the stamp and exhibits a lateral connectivity allowing molecular transport across the topographic boundaries. Applications of these bilayer-coated elastomeric stamps in microcontact printing of lipid bilayers reveal a Fluid-tearing process wherein the bilayer in contact regions selectively tra...
Eric L. Kendall - One of the best experts on this subject based on the ideXlab platform.
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Lithographically Defined Macroscale Modulation of Lateral Fluidity and Phase Separation Realized via Patterned Nanoporous Silica-Supported Phospholipid Bilayers
2016Co-Authors: Eric L. Kendall, Viviane N. Ngassam, Sean F. Gilmore, Jeffrey C. Brinker, Atul N. ParikhAbstract:Using lithographically defined surfaces consisting of hydrophilic patterns of nanoporous and nonporous (bulk) amorphous silica, we show that fusion of small, unilamellar lipid vesicles produces a single, Contiguous, Fluid bilayer phase experiencing a predetermined pattern of interfacial interactions. Although long-range lateral Fluidity of the bilayer, characterized by fluorescence recovery after photobleaching, indicates a nominally single average diffusion constant, fluorescence microscopy-based measurements of temperature-dependent onset of Fluidity reveals a locally enhanced Fluidity for bilayer regions supported on nanoporous silica in the vicinity of the Fluid–gel transition temperature. Furthermore, thermally quenching lipid bilayers composed of a binary lipid mixture below its apparent miscibility transition temperature induces qualitatively different lateral phase separation in each region of the supported bilayer: The nanoporous substrate produces large, microscopic domains (and domain-aggregates), whereas surface texture characterized by much smaller domains and devoid of any domain-aggregates appears on bulk glass-supported regions of the single-lipid bilayer. Interestingly, lateral distribution of the constituent molecules also reveals an enrichment of gel-phase lipids over nanoporous regions, presumably as a consequence of differential mobilities of constituent lipids across the topographic bulk/nanoporous boundary. Together, these results reveal that subtle local variations in constraints imposed at the bilayer interface, such as by spatial variations in roughness and substrate adhesion, can give rise to significant differences in macroscale biophysical properties of phospholipid bilayers even within a single, Contiguous phase
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Lithographically Defined Macroscale Modulation of Lateral Fluidity and Phase Separation Realized via Patterned Nanoporous Silica-Supported Phospholipid Bilayers
Journal of the American Chemical Society, 2013Co-Authors: Eric L. Kendall, Viviane N. Ngassam, Sean F. Gilmore, C. Jeffrey Brinker, Atul N. ParikhAbstract:Using lithographically defined surfaces consisting of hydrophilic patterns of nanoporous and nonporous (bulk) amorphous silica, we show that fusion of small, unilamellar lipid vesicles produces a single, Contiguous, Fluid bilayer phase experiencing a predetermined pattern of interfacial interactions. Although long-range lateral Fluidity of the bilayer, characterized by fluorescence recovery after photobleaching, indicates a nominally single average diffusion constant, fluorescence microscopy-based measurements of temperature-dependent onset of Fluidity reveals a locally enhanced Fluidity for bilayer regions supported on nanoporous silica in the vicinity of the Fluid–gel transition temperature. Furthermore, thermally quenching lipid bilayers composed of a binary lipid mixture below its apparent miscibility transition temperature induces qualitatively different lateral phase separation in each region of the supported bilayer: The nanoporous substrate produces large, microscopic domains (and domain-aggregate...
Viviane N. Ngassam - One of the best experts on this subject based on the ideXlab platform.
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Lithographically Defined Macroscale Modulation of Lateral Fluidity and Phase Separation Realized via Patterned Nanoporous Silica-Supported Phospholipid Bilayers
2016Co-Authors: Eric L. Kendall, Viviane N. Ngassam, Sean F. Gilmore, Jeffrey C. Brinker, Atul N. ParikhAbstract:Using lithographically defined surfaces consisting of hydrophilic patterns of nanoporous and nonporous (bulk) amorphous silica, we show that fusion of small, unilamellar lipid vesicles produces a single, Contiguous, Fluid bilayer phase experiencing a predetermined pattern of interfacial interactions. Although long-range lateral Fluidity of the bilayer, characterized by fluorescence recovery after photobleaching, indicates a nominally single average diffusion constant, fluorescence microscopy-based measurements of temperature-dependent onset of Fluidity reveals a locally enhanced Fluidity for bilayer regions supported on nanoporous silica in the vicinity of the Fluid–gel transition temperature. Furthermore, thermally quenching lipid bilayers composed of a binary lipid mixture below its apparent miscibility transition temperature induces qualitatively different lateral phase separation in each region of the supported bilayer: The nanoporous substrate produces large, microscopic domains (and domain-aggregates), whereas surface texture characterized by much smaller domains and devoid of any domain-aggregates appears on bulk glass-supported regions of the single-lipid bilayer. Interestingly, lateral distribution of the constituent molecules also reveals an enrichment of gel-phase lipids over nanoporous regions, presumably as a consequence of differential mobilities of constituent lipids across the topographic bulk/nanoporous boundary. Together, these results reveal that subtle local variations in constraints imposed at the bilayer interface, such as by spatial variations in roughness and substrate adhesion, can give rise to significant differences in macroscale biophysical properties of phospholipid bilayers even within a single, Contiguous phase
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Lithographically Defined Macroscale Modulation of Lateral Fluidity and Phase Separation Realized via Patterned Nanoporous Silica-Supported Phospholipid Bilayers
Journal of the American Chemical Society, 2013Co-Authors: Eric L. Kendall, Viviane N. Ngassam, Sean F. Gilmore, C. Jeffrey Brinker, Atul N. ParikhAbstract:Using lithographically defined surfaces consisting of hydrophilic patterns of nanoporous and nonporous (bulk) amorphous silica, we show that fusion of small, unilamellar lipid vesicles produces a single, Contiguous, Fluid bilayer phase experiencing a predetermined pattern of interfacial interactions. Although long-range lateral Fluidity of the bilayer, characterized by fluorescence recovery after photobleaching, indicates a nominally single average diffusion constant, fluorescence microscopy-based measurements of temperature-dependent onset of Fluidity reveals a locally enhanced Fluidity for bilayer regions supported on nanoporous silica in the vicinity of the Fluid–gel transition temperature. Furthermore, thermally quenching lipid bilayers composed of a binary lipid mixture below its apparent miscibility transition temperature induces qualitatively different lateral phase separation in each region of the supported bilayer: The nanoporous substrate produces large, microscopic domains (and domain-aggregate...
Sean F. Gilmore - One of the best experts on this subject based on the ideXlab platform.
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Lithographically Defined Macroscale Modulation of Lateral Fluidity and Phase Separation Realized via Patterned Nanoporous Silica-Supported Phospholipid Bilayers
2016Co-Authors: Eric L. Kendall, Viviane N. Ngassam, Sean F. Gilmore, Jeffrey C. Brinker, Atul N. ParikhAbstract:Using lithographically defined surfaces consisting of hydrophilic patterns of nanoporous and nonporous (bulk) amorphous silica, we show that fusion of small, unilamellar lipid vesicles produces a single, Contiguous, Fluid bilayer phase experiencing a predetermined pattern of interfacial interactions. Although long-range lateral Fluidity of the bilayer, characterized by fluorescence recovery after photobleaching, indicates a nominally single average diffusion constant, fluorescence microscopy-based measurements of temperature-dependent onset of Fluidity reveals a locally enhanced Fluidity for bilayer regions supported on nanoporous silica in the vicinity of the Fluid–gel transition temperature. Furthermore, thermally quenching lipid bilayers composed of a binary lipid mixture below its apparent miscibility transition temperature induces qualitatively different lateral phase separation in each region of the supported bilayer: The nanoporous substrate produces large, microscopic domains (and domain-aggregates), whereas surface texture characterized by much smaller domains and devoid of any domain-aggregates appears on bulk glass-supported regions of the single-lipid bilayer. Interestingly, lateral distribution of the constituent molecules also reveals an enrichment of gel-phase lipids over nanoporous regions, presumably as a consequence of differential mobilities of constituent lipids across the topographic bulk/nanoporous boundary. Together, these results reveal that subtle local variations in constraints imposed at the bilayer interface, such as by spatial variations in roughness and substrate adhesion, can give rise to significant differences in macroscale biophysical properties of phospholipid bilayers even within a single, Contiguous phase
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Lithographically Defined Macroscale Modulation of Lateral Fluidity and Phase Separation Realized via Patterned Nanoporous Silica-Supported Phospholipid Bilayers
Journal of the American Chemical Society, 2013Co-Authors: Eric L. Kendall, Viviane N. Ngassam, Sean F. Gilmore, C. Jeffrey Brinker, Atul N. ParikhAbstract:Using lithographically defined surfaces consisting of hydrophilic patterns of nanoporous and nonporous (bulk) amorphous silica, we show that fusion of small, unilamellar lipid vesicles produces a single, Contiguous, Fluid bilayer phase experiencing a predetermined pattern of interfacial interactions. Although long-range lateral Fluidity of the bilayer, characterized by fluorescence recovery after photobleaching, indicates a nominally single average diffusion constant, fluorescence microscopy-based measurements of temperature-dependent onset of Fluidity reveals a locally enhanced Fluidity for bilayer regions supported on nanoporous silica in the vicinity of the Fluid–gel transition temperature. Furthermore, thermally quenching lipid bilayers composed of a binary lipid mixture below its apparent miscibility transition temperature induces qualitatively different lateral phase separation in each region of the supported bilayer: The nanoporous substrate produces large, microscopic domains (and domain-aggregate...
C. Jeffrey Brinker - One of the best experts on this subject based on the ideXlab platform.
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Lithographically Defined Macroscale Modulation of Lateral Fluidity and Phase Separation Realized via Patterned Nanoporous Silica-Supported Phospholipid Bilayers
Journal of the American Chemical Society, 2013Co-Authors: Eric L. Kendall, Viviane N. Ngassam, Sean F. Gilmore, C. Jeffrey Brinker, Atul N. ParikhAbstract:Using lithographically defined surfaces consisting of hydrophilic patterns of nanoporous and nonporous (bulk) amorphous silica, we show that fusion of small, unilamellar lipid vesicles produces a single, Contiguous, Fluid bilayer phase experiencing a predetermined pattern of interfacial interactions. Although long-range lateral Fluidity of the bilayer, characterized by fluorescence recovery after photobleaching, indicates a nominally single average diffusion constant, fluorescence microscopy-based measurements of temperature-dependent onset of Fluidity reveals a locally enhanced Fluidity for bilayer regions supported on nanoporous silica in the vicinity of the Fluid–gel transition temperature. Furthermore, thermally quenching lipid bilayers composed of a binary lipid mixture below its apparent miscibility transition temperature induces qualitatively different lateral phase separation in each region of the supported bilayer: The nanoporous substrate produces large, microscopic domains (and domain-aggregate...