The Experts below are selected from a list of 360 Experts worldwide ranked by ideXlab platform
Joseph J. Falke - One of the best experts on this subject based on the ideXlab platform.
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engineered socket study of signaling through a four helix bundle evidence for a yin yang mechanism in the kinase Control Module of the aspartate receptor
Biochemistry, 2009Co-Authors: Kalin E Swain, Miguel A Gonzalez, Joseph J. FalkeAbstract:The chemoreceptors of Escherichia coli and Salmonella typhimurium form stable oligomers that associate with the coupling protein CheW and the histidine kinase CheA to form an ultrasensitive, ultrastable signaling lattice. Attractant binding to the periplasmic domain of a given receptor dimer triggers a transmembrane conformational change transmitted through the receptor to its cytoplasmic kinase Control Module, a long four-helix bundle that binds and regulates CheA kinase. The kinase Control Module comprises three functional regions: the adaptation region possessing the receptor adaptation sites, a coupling region that transmits signals between other regions, and the protein interaction region possessing contact sites for receptor oligomerization and for CheA−CheW binding. On the basis of the spatial clustering of known signal locking Cys substitutions and engineered disulfide bonds, this study develops the yin−yang hypothesis for signal transmission through the kinase Control Module. This hypothesis prop...
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Engineered socket study of signaling through a four-helix bundle: evidence for a yin-yang mechanism in the kinase Control Module of the aspartate receptor.
Biochemistry, 2009Co-Authors: Kalin E Swain, Miguel A Gonzalez, Joseph J. FalkeAbstract:The chemoreceptors of Escherichia coli and Salmonella typhimurium form stable oligomers that associate with the coupling protein CheW and the histidine kinase CheA to form an ultrasensitive, ultrastable signaling lattice. Attractant binding to the periplasmic domain of a given receptor dimer triggers a transmembrane conformational change transmitted through the receptor to its cytoplasmic kinase Control Module, a long four-helix bundle that binds and regulates CheA kinase. The kinase Control Module comprises three functional regions: the adaptation region possessing the receptor adaptation sites, a coupling region that transmits signals between other regions, and the protein interaction region possessing contact sites for receptor oligomerization and for CheA-CheW binding. On the basis of the spatial clustering of known signal locking Cys substitutions and engineered disulfide bonds, this study develops the yin-yang hypothesis for signal transmission through the kinase Control Module. This hypothesis proposes that signals are transmitted through the four-helix bundle via changes in helix-helix packing and that the helix packing changes in the adaptation and protein interaction regions are tightly and antisymmetrically coupled. Specifically, strong helix packing in the adaptation region stabilizes the receptor on state, while strong helix packing in the protein interaction region stabilizes the off state. To test the yin-yang hypothesis, conserved sockets likely to strengthen specific helix-helix contacts via knob-in-hole packing interactions were identified in the adaptation, coupling, and protein interaction regions. For 32 sockets, the knob side chain was truncated to Ala to weaken the knob-in-hole packing and thereby destabilize the local helix-helix interaction provided by that socket. We term this approach a "knob truncation scan". Of the 32 knob truncations, 28 yielded stable receptors. Functional analysis of the signaling state of these receptors revealed seven lock-off knob truncations, all located in the adaptation region, that trap the receptor in its "off" signaling state (low kinase activity, high methylation activity). Also revealed were five lock-on knob truncations, all located in the protein interaction region, that trap the "on" state (high kinase activity, low methylation activity). These findings provide strong evidence that a yin-yang coupling mechanism generates concerted, antisymmetric helix-helix packing changes within the adaptation and protein interaction regions during receptor on-off switching. Conserved sockets that stabilize local helix-helix interactions play a central role in this mechanism: in the on state, sockets are formed in the adaptation region and disrupted in the protein interaction region, while the opposite is true in the off state.
Kalin E Swain - One of the best experts on this subject based on the ideXlab platform.
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engineered socket study of signaling through a four helix bundle evidence for a yin yang mechanism in the kinase Control Module of the aspartate receptor
Biochemistry, 2009Co-Authors: Kalin E Swain, Miguel A Gonzalez, Joseph J. FalkeAbstract:The chemoreceptors of Escherichia coli and Salmonella typhimurium form stable oligomers that associate with the coupling protein CheW and the histidine kinase CheA to form an ultrasensitive, ultrastable signaling lattice. Attractant binding to the periplasmic domain of a given receptor dimer triggers a transmembrane conformational change transmitted through the receptor to its cytoplasmic kinase Control Module, a long four-helix bundle that binds and regulates CheA kinase. The kinase Control Module comprises three functional regions: the adaptation region possessing the receptor adaptation sites, a coupling region that transmits signals between other regions, and the protein interaction region possessing contact sites for receptor oligomerization and for CheA−CheW binding. On the basis of the spatial clustering of known signal locking Cys substitutions and engineered disulfide bonds, this study develops the yin−yang hypothesis for signal transmission through the kinase Control Module. This hypothesis prop...
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Engineered socket study of signaling through a four-helix bundle: evidence for a yin-yang mechanism in the kinase Control Module of the aspartate receptor.
Biochemistry, 2009Co-Authors: Kalin E Swain, Miguel A Gonzalez, Joseph J. FalkeAbstract:The chemoreceptors of Escherichia coli and Salmonella typhimurium form stable oligomers that associate with the coupling protein CheW and the histidine kinase CheA to form an ultrasensitive, ultrastable signaling lattice. Attractant binding to the periplasmic domain of a given receptor dimer triggers a transmembrane conformational change transmitted through the receptor to its cytoplasmic kinase Control Module, a long four-helix bundle that binds and regulates CheA kinase. The kinase Control Module comprises three functional regions: the adaptation region possessing the receptor adaptation sites, a coupling region that transmits signals between other regions, and the protein interaction region possessing contact sites for receptor oligomerization and for CheA-CheW binding. On the basis of the spatial clustering of known signal locking Cys substitutions and engineered disulfide bonds, this study develops the yin-yang hypothesis for signal transmission through the kinase Control Module. This hypothesis proposes that signals are transmitted through the four-helix bundle via changes in helix-helix packing and that the helix packing changes in the adaptation and protein interaction regions are tightly and antisymmetrically coupled. Specifically, strong helix packing in the adaptation region stabilizes the receptor on state, while strong helix packing in the protein interaction region stabilizes the off state. To test the yin-yang hypothesis, conserved sockets likely to strengthen specific helix-helix contacts via knob-in-hole packing interactions were identified in the adaptation, coupling, and protein interaction regions. For 32 sockets, the knob side chain was truncated to Ala to weaken the knob-in-hole packing and thereby destabilize the local helix-helix interaction provided by that socket. We term this approach a "knob truncation scan". Of the 32 knob truncations, 28 yielded stable receptors. Functional analysis of the signaling state of these receptors revealed seven lock-off knob truncations, all located in the adaptation region, that trap the receptor in its "off" signaling state (low kinase activity, high methylation activity). Also revealed were five lock-on knob truncations, all located in the protein interaction region, that trap the "on" state (high kinase activity, low methylation activity). These findings provide strong evidence that a yin-yang coupling mechanism generates concerted, antisymmetric helix-helix packing changes within the adaptation and protein interaction regions during receptor on-off switching. Conserved sockets that stabilize local helix-helix interactions play a central role in this mechanism: in the on state, sockets are formed in the adaptation region and disrupted in the protein interaction region, while the opposite is true in the off state.
Miguel A Gonzalez - One of the best experts on this subject based on the ideXlab platform.
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engineered socket study of signaling through a four helix bundle evidence for a yin yang mechanism in the kinase Control Module of the aspartate receptor
Biochemistry, 2009Co-Authors: Kalin E Swain, Miguel A Gonzalez, Joseph J. FalkeAbstract:The chemoreceptors of Escherichia coli and Salmonella typhimurium form stable oligomers that associate with the coupling protein CheW and the histidine kinase CheA to form an ultrasensitive, ultrastable signaling lattice. Attractant binding to the periplasmic domain of a given receptor dimer triggers a transmembrane conformational change transmitted through the receptor to its cytoplasmic kinase Control Module, a long four-helix bundle that binds and regulates CheA kinase. The kinase Control Module comprises three functional regions: the adaptation region possessing the receptor adaptation sites, a coupling region that transmits signals between other regions, and the protein interaction region possessing contact sites for receptor oligomerization and for CheA−CheW binding. On the basis of the spatial clustering of known signal locking Cys substitutions and engineered disulfide bonds, this study develops the yin−yang hypothesis for signal transmission through the kinase Control Module. This hypothesis prop...
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Engineered socket study of signaling through a four-helix bundle: evidence for a yin-yang mechanism in the kinase Control Module of the aspartate receptor.
Biochemistry, 2009Co-Authors: Kalin E Swain, Miguel A Gonzalez, Joseph J. FalkeAbstract:The chemoreceptors of Escherichia coli and Salmonella typhimurium form stable oligomers that associate with the coupling protein CheW and the histidine kinase CheA to form an ultrasensitive, ultrastable signaling lattice. Attractant binding to the periplasmic domain of a given receptor dimer triggers a transmembrane conformational change transmitted through the receptor to its cytoplasmic kinase Control Module, a long four-helix bundle that binds and regulates CheA kinase. The kinase Control Module comprises three functional regions: the adaptation region possessing the receptor adaptation sites, a coupling region that transmits signals between other regions, and the protein interaction region possessing contact sites for receptor oligomerization and for CheA-CheW binding. On the basis of the spatial clustering of known signal locking Cys substitutions and engineered disulfide bonds, this study develops the yin-yang hypothesis for signal transmission through the kinase Control Module. This hypothesis proposes that signals are transmitted through the four-helix bundle via changes in helix-helix packing and that the helix packing changes in the adaptation and protein interaction regions are tightly and antisymmetrically coupled. Specifically, strong helix packing in the adaptation region stabilizes the receptor on state, while strong helix packing in the protein interaction region stabilizes the off state. To test the yin-yang hypothesis, conserved sockets likely to strengthen specific helix-helix contacts via knob-in-hole packing interactions were identified in the adaptation, coupling, and protein interaction regions. For 32 sockets, the knob side chain was truncated to Ala to weaken the knob-in-hole packing and thereby destabilize the local helix-helix interaction provided by that socket. We term this approach a "knob truncation scan". Of the 32 knob truncations, 28 yielded stable receptors. Functional analysis of the signaling state of these receptors revealed seven lock-off knob truncations, all located in the adaptation region, that trap the receptor in its "off" signaling state (low kinase activity, high methylation activity). Also revealed were five lock-on knob truncations, all located in the protein interaction region, that trap the "on" state (high kinase activity, low methylation activity). These findings provide strong evidence that a yin-yang coupling mechanism generates concerted, antisymmetric helix-helix packing changes within the adaptation and protein interaction regions during receptor on-off switching. Conserved sockets that stabilize local helix-helix interactions play a central role in this mechanism: in the on state, sockets are formed in the adaptation region and disrupted in the protein interaction region, while the opposite is true in the off state.
Peng Peng - One of the best experts on this subject based on the ideXlab platform.
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intelligent substation relay protection noninvasive test system and method
2014Co-Authors: Gao Xiang, Peng PengAbstract:The invention relates to an intelligent substation relay protection noninvasive test system and an intelligent substation relay protection noninvasive test method, which belong to the technical field of electric systems. The intelligent substation relay protection noninvasive test system comprises a network communication Module, an SCD analysis Module, a test Control Module, a test excitation Module and a test evaluation Module, wherein the SCD analysis Module, the test Control Module, the test excitation Module and the test evaluation Module are respectively connected with the network communication Module; the SCD analysis Module is respectively connected with the network communication Module, the test evaluation Module and an HMI for transmitting an SCD file; the network communication Module is respectively connected with an MMS network and a GOOSE network for transmitting the SCD file, a test instruction and a test case; the test Control Module is respectively connected with the HMI, the test excitation Module and the network communication Module for transmitting the test instruction, and the test Control Module is connected with the test evaluation Module for transmitting a test requirement; the test excitation Module is used for receiving the test instruction and outputting the test case to the network Through the system and the method, tests on an SV chain circuit between a merging unit and a protection device as well as a GOOSE chain circuit between the protection device and an intelligent terminal can be implemented, and the test efficiency of an intelligent substation is improved.
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intelligent substation relay protection noninvasive test system and method
2014Co-Authors: Gao Xiang, Peng PengAbstract:The invention relates to an intelligent substation relay protection noninvasive test system and an intelligent substation relay protection noninvasive test method, which belong to the technical field of electric systems. The intelligent substation relay protection noninvasive test system comprises a network communication Module, an SCD analysis Module, a test Control Module, a test excitation Module and a test evaluation Module, wherein the SCD analysis Module, the test Control Module, the test excitation Module and the test evaluation Module are respectively connected with the network communication Module; the SCD analysis Module is respectively connected with the network communication Module, the test evaluation Module and an HMI for transmitting an SCD file; the network communication Module is respectively connected with an MMS network and a GOOSE network for transmitting the SCD file, a test instruction and a test case; the test Control Module is respectively connected with the HMI, the test excitation Module and the network communication Module for transmitting the test instruction, and the test Control Module is connected with the test evaluation Module for transmitting a test requirement; the test excitation Module is used for receiving the test instruction and outputting the test case to the network Through the system and the method, tests on an SV chain circuit between a merging unit and a protection device as well as a GOOSE chain circuit between the protection device and an intelligent terminal can be implemented, and the test efficiency of an intelligent substation is improved.
Cheng-huei Yang - One of the best experts on this subject based on the ideXlab platform.
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Morse code application for wireless environmental Control systems for severely disabled individuals
IEEE Transactions on Neural Systems and Rehabilitation Engineering, 2003Co-Authors: Cheng-hong Yang, Li-yeh Chuang, Cheng-huei YangAbstract:Some physically-disabled people with neuromuscular diseases such as amyotrophic lateral sclerosis, multiple sclerosis, muscular dystrophy, or other conditions that hinder their ability to write, type, and speak, require an assistive tool for purposes of augmentative and alternative communication in their daily lives. In this paper, we designed and implemented a wireless environmental Control system using Morse code as an adapted access communication tool. The proposed system includes four parts: input-Control Module; recognition Module; wireless-Control Module; and electronic-equipment-Control Module. The signals are transmitted using adopted radio frequencies, which permits long distance transmission without space limitation. Experimental results revealed that three participants with physical handicaps were able to gain access to electronic facilities after two months' practice with the new system.