The Experts below are selected from a list of 72 Experts worldwide ranked by ideXlab platform
Chantal J Fregeau - One of the best experts on this subject based on the ideXlab platform.
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Performance of Identifiler Direct and PowerPlex 16 HS on the Applied Biosystems 3730 DNA Analyzer for processing biological samples archived on FTA cards.
Forensic science international. Genetics, 2012Co-Authors: N Laurin, Anick Demoors, Chantal J FregeauAbstract:Direct amplification of STR loci from biological samples collected on FTA cards without prior DNA purification was evaluated using Identifiler Direct and PowerPlex 16 HS in conjunction with the use of a high throughput Applied Biosystems 3730 DNA Analyzer. In order to reduce the overall sample processing cost, reduced PCR volumes combined with various FTA disk sizes were tested. Optimized STR profiles were obtained using a 0.53 mm disk size in 10 μL PCR volume for both STR systems. These protocols proved effective in generating high quality profiles on the 3730 DNA Analyzer from both blood and buccal FTA samples. Reproducibility, concordance, robustness, sample stability and profile quality were assessed using a collection of blood and buccal samples on FTA cards from volunteer donors as well as from Convicted Offenders. The new developed protocols offer enhanced throughput capability and cost effectiveness without compromising the robustness and quality of the STR profiles obtained. These results support the use of these protocols for processing Convicted Offender samples submitted to the National DNA Data Bank of Canada. Similar protocols could be applied to the processing of casework reference samples or in paternity or family relationship testing.
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new validated analytical process for Convicted Offender samples submitted to the canadian national dna data bank
Forensic Science International: Genetics Supplement Series, 2011Co-Authors: N Laurin, A De Moors, Chantal J FregeauAbstract:Abstract Optimization and validation of direct amplification protocols for both AmpF l STR ® Identifiler ® Direct (IDD) and PowerPlex ® 16 HS (PP16HS) on the high-throughput 3730 DNA Analyzer (Applied Biosystems) were carried out in preparation for potential changes in Canadian legislation. Amplification conditions were optimized to perform direct amplification of single source blood, buccal and extracted hair roots collected from Convicted Offenders (CO) on FTA ® paper without the need for sample purification prior to amplification. Validation was performed using IDD and PP16HS on a wide collection of blood and buccal samples from volunteer donors. In addition, 111 CO blood samples were selected to assess the robustness of the new analytical process (i.e. 0.53mm FTA disks amplified in 10μL PCR volume) and verify the stability of DNA stored on FTA paper at room temperature for up to 10 years. Studies such as reproducibility, robustness and concordance were carried out. The quality of the STR profiles generated using the new analytical process was assessed by examining profile intensity, heterozygote peak height ratios and interlocus balance. Reliable and high quality profiles from all CO samples and volunteer samples were produced under the new process giving support to its implementation.
N Laurin - One of the best experts on this subject based on the ideXlab platform.
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Performance of Identifiler Direct and PowerPlex 16 HS on the Applied Biosystems 3730 DNA Analyzer for processing biological samples archived on FTA cards.
Forensic science international. Genetics, 2012Co-Authors: N Laurin, Anick Demoors, Chantal J FregeauAbstract:Direct amplification of STR loci from biological samples collected on FTA cards without prior DNA purification was evaluated using Identifiler Direct and PowerPlex 16 HS in conjunction with the use of a high throughput Applied Biosystems 3730 DNA Analyzer. In order to reduce the overall sample processing cost, reduced PCR volumes combined with various FTA disk sizes were tested. Optimized STR profiles were obtained using a 0.53 mm disk size in 10 μL PCR volume for both STR systems. These protocols proved effective in generating high quality profiles on the 3730 DNA Analyzer from both blood and buccal FTA samples. Reproducibility, concordance, robustness, sample stability and profile quality were assessed using a collection of blood and buccal samples on FTA cards from volunteer donors as well as from Convicted Offenders. The new developed protocols offer enhanced throughput capability and cost effectiveness without compromising the robustness and quality of the STR profiles obtained. These results support the use of these protocols for processing Convicted Offender samples submitted to the National DNA Data Bank of Canada. Similar protocols could be applied to the processing of casework reference samples or in paternity or family relationship testing.
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new validated analytical process for Convicted Offender samples submitted to the canadian national dna data bank
Forensic Science International: Genetics Supplement Series, 2011Co-Authors: N Laurin, A De Moors, Chantal J FregeauAbstract:Abstract Optimization and validation of direct amplification protocols for both AmpF l STR ® Identifiler ® Direct (IDD) and PowerPlex ® 16 HS (PP16HS) on the high-throughput 3730 DNA Analyzer (Applied Biosystems) were carried out in preparation for potential changes in Canadian legislation. Amplification conditions were optimized to perform direct amplification of single source blood, buccal and extracted hair roots collected from Convicted Offenders (CO) on FTA ® paper without the need for sample purification prior to amplification. Validation was performed using IDD and PP16HS on a wide collection of blood and buccal samples from volunteer donors. In addition, 111 CO blood samples were selected to assess the robustness of the new analytical process (i.e. 0.53mm FTA disks amplified in 10μL PCR volume) and verify the stability of DNA stored on FTA paper at room temperature for up to 10 years. Studies such as reproducibility, robustness and concordance were carried out. The quality of the STR profiles generated using the new analytical process was assessed by examining profile intensity, heterozygote peak height ratios and interlocus balance. Reliable and high quality profiles from all CO samples and volunteer samples were produced under the new process giving support to its implementation.
Samuel T.g. Ferreira - One of the best experts on this subject based on the ideXlab platform.
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increasing Convicted Offender genetic profiles in the brazilian national dna database legislation projects and perspectives
Forensic Science International: Genetics Supplement Series, 2019Co-Authors: Aline Costa Minervino, Ronaldo C. Silva, Mariana F. Da Mota, Cecília H.f. Matte, Daniela Koshikene, João Paulo S.c. Oliveira, Tatiana Hessab, Bruno Trindade, Guilherme S. Jacques, Samuel T.g. FerreiraAbstract:Abstract Brazilian legislation determines that individuals must obligatorily be included in DNA databases in cases of convictions for heinous or wilful violent crimes. In 2017, just over 2,000 individuals had their genetic profiles inserted into DNA databases. However, it is estimated that 137,600 individuals should be identified by genetic profiles. In early 2018, the Convict Genetic Profile Identification Project was started. It aims to obey the law, to reach the strategic goal of insert convicts' profiles in DNA databases to 50% (N = 68,670) and to promote the integration between Brazilian Forensic DNA Laboratories. There has been a growth of over 1189% in Convicted Offender genetic profile in RIBPG (2,008 on November 29, 2017, compared to 25,875 on August 28, 2019). Although these represent about 38% of the target, the forensic DNA laboratories reported they have already collected about 79% convicts’ samples. It is expected that the project's target will be reached by the end of 2019.
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Increasing Convicted Offender genetic profiles in the Brazilian National DNA Database—Legislation, projects and perspectives
Forensic Science International: Genetics Supplement Series, 2019Co-Authors: Aline Costa Minervino, Ronaldo C. Silva, Mariana F. Da Mota, Cecília H.f. Matte, Daniela Koshikene, João Paulo S.c. Oliveira, Tatiana Hessab, Bruno Trindade, Guilherme S. Jacques, Samuel T.g. FerreiraAbstract:Abstract Brazilian legislation determines that individuals must obligatorily be included in DNA databases in cases of convictions for heinous or wilful violent crimes. In 2017, just over 2,000 individuals had their genetic profiles inserted into DNA databases. However, it is estimated that 137,600 individuals should be identified by genetic profiles. In early 2018, the Convict Genetic Profile Identification Project was started. It aims to obey the law, to reach the strategic goal of insert convicts' profiles in DNA databases to 50% (N = 68,670) and to promote the integration between Brazilian Forensic DNA Laboratories. There has been a growth of over 1189% in Convicted Offender genetic profile in RIBPG (2,008 on November 29, 2017, compared to 25,875 on August 28, 2019). Although these represent about 38% of the target, the forensic DNA laboratories reported they have already collected about 79% convicts’ samples. It is expected that the project's target will be reached by the end of 2019.
Karl Reich - One of the best experts on this subject based on the ideXlab platform.
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automated forensic dna purification optimized for fta card punches and identifiler str based pcr analysis
Clinics in Laboratory Medicine, 2007Co-Authors: Lois C Tack, Michelle Thomas, Karl ReichAbstract:Forensic labs globally face the same problem—a growing need to process a greater number and wider variety of samples for DNA analysis. The same forensic lab can be tasked all at once with processing mixed casework samples from crime scenes, Convicted Offender samples for database entry, and tissue from tsunami victims for identification. Besides flexibility in the robotic system chosen for forensic automation, there is a need, for each sample type, to develop new methodology that is not only faster but also more reliable than past procedures. FTA is a chemical treatment of paper, unique to Whatman Bioscience, and is used for the stabilization and storage of biological samples. Here, the authors describe optimization of the Whatman FTA Purification Kit protocol for use with the AmpFlSTR Identifiler PCR Amplification Kit.
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automated forensic dna purification optimized for fta card punches and identifiler str based pcr analysis
Journal of Laboratory Automation, 2005Co-Authors: Lois C Tack, Michelle Thomas, Karl ReichAbstract:Forensic labs globally face the same problem—a growing need to process a greater number and wider variety of samples for DNA analysis. The same forensic lab can be tasked all at once with processing mixed casework samples from crime scenes, Convicted Offender samples for database entry, and tissue from tsunami victims for identification. Besides flexibility in the robotic system chosen for forensic automation, there is a need, for each sample type, to develop new methodology that is not only faster but also more reliable than past procedures. FTA is a chemical treatment of paper, unique to whatman Bioscience, and is used for the stabilization and storage of biological samples. Here, we describe optimization of the Whatman FTA Purification Kit protocol for use with the AmpFlSTR Identifiler PCR Amplification Kit. The conditions giving the best quality Identifiler results were used to automate a simple rapid method that processed forensic samples applied to FTA-treated paper punches in a 96-well plate in ∼30...
Anick Demoors - One of the best experts on this subject based on the ideXlab platform.
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Performance of Identifiler Direct and PowerPlex 16 HS on the Applied Biosystems 3730 DNA Analyzer for processing biological samples archived on FTA cards.
Forensic science international. Genetics, 2012Co-Authors: N Laurin, Anick Demoors, Chantal J FregeauAbstract:Direct amplification of STR loci from biological samples collected on FTA cards without prior DNA purification was evaluated using Identifiler Direct and PowerPlex 16 HS in conjunction with the use of a high throughput Applied Biosystems 3730 DNA Analyzer. In order to reduce the overall sample processing cost, reduced PCR volumes combined with various FTA disk sizes were tested. Optimized STR profiles were obtained using a 0.53 mm disk size in 10 μL PCR volume for both STR systems. These protocols proved effective in generating high quality profiles on the 3730 DNA Analyzer from both blood and buccal FTA samples. Reproducibility, concordance, robustness, sample stability and profile quality were assessed using a collection of blood and buccal samples on FTA cards from volunteer donors as well as from Convicted Offenders. The new developed protocols offer enhanced throughput capability and cost effectiveness without compromising the robustness and quality of the STR profiles obtained. These results support the use of these protocols for processing Convicted Offender samples submitted to the National DNA Data Bank of Canada. Similar protocols could be applied to the processing of casework reference samples or in paternity or family relationship testing.