The Experts below are selected from a list of 324 Experts worldwide ranked by ideXlab platform

Lepoint Gilles - One of the best experts on this subject based on the ideXlab platform.

  • Trophic diversity of seagrass detritus copepods: A consequence of species-specific specialization or a random diet?
    'PeerJ', 2016
    Co-Authors: Mascart Thibaud, De Troch Marleen, Remy François, Michel Loïc, Lepoint Gilles
    Abstract:

    audience: researcher, professional, studentOne of the major ecological research questions is understanding how biodiversity influences ecosystem functioning. Unravelling interspecific feeding preferences of organisms with overlapping trophic niches will give part of the answer. Subsequently, the present study displays the trophic diversity of a benthic copepod community in a North-Western Corsican Posidonia oceanica seagrass ecosystem. These seagrass meadows are often interrupted by bare sand patches serving as deposition area for loose detritus. The accumulated macrophytodetritus, mainly derived from senescent macrophytes, harbour a diverse community of Harpacticoida (Crustacea, Copepoda). The most abundant copepods (i.e. three harpacticoids and one calanoid, belonging to different eco-morphological types) and their potential food sources (i.e. macrophytodetritus, epiphytic biofilm and suspended organic matter) were analysed for stable isotope ratios (δ13C, δ15N) and total lipids content. The results revealed a harpacticoid copepod feeding preference towards the epiphytic biofilm, while calanoid copepods preferred suspended organic matter. Additionally, a species-specific composition variation revealed finer partitioning of food resources (e.g. different micro-organisms present in the biofilm like bacteria, diatoms, fungi) over time.In conclusion, results showed species-specific food preferences, resulting in trophic niche and resource partitioning. Every eco-morphological type seems to cope in different ways with temporal fluctuations of food sources to comply with their nutritional needs. This illustrates the high resilience of the copepod community present in macrophytodetritus accumulations. Moreover, our results underlined the importance of multiple biomarker species-specific analysis in trophic ecology studies, especially in complex and dynamic environments offering numerous food items to consumers

  • Trophic diversity of seagrass detritus copepods: A consequence of species-specific specialization or a random diet?
    2016
    Co-Authors: Mascart Thibaud, De Troch Marleen, Remy François, Michel Loïc, Lepoint Gilles
    Abstract:

    One of the major ecological research questions is understanding how biodiversity influences ecosystem functioning. Unravelling interspecific feeding preferences of organisms with overlapping trophic niches will give part of the answer. Subsequently, the present study displays the trophic diversity of a benthic copepod community in a North-Western Corsican Posidonia oceanica seagrass ecosystem. These seagrass meadows are often interrupted by bare sand patches serving as deposition area for loose detritus. The accumulated macrophytodetritus, mainly derived from senescent macrophytes, harbour a diverse community of Harpacticoida (Crustacea, Copepoda). The most abundant copepods (i.e. three harpacticoids and one calanoid, belonging to different eco-morphological types) and their potential food sources (i.e. macrophytodetritus, epiphytic biofilm and suspended organic matter) were analysed for stable isotope ratios (δ13C, δ15N) and total lipids content. The results revealed a harpacticoid copepod feeding preference towards the epiphytic biofilm, while calanoid copepods preferred suspended organic matter. Additionally, a species-specific composition variation revealed finer partitioning of food resources (e.g. different micro-organisms present in the biofilm like bacteria, diatoms, fungi) over time.In conclusion, results showed species-specific food preferences, resulting in trophic niche and resource partitioning. Every eco-morphological type seems to cope in different ways with temporal fluctuations of food sources to comply with their nutritional needs. This illustrates the high resilience of the copepod community present in macrophytodetritus accumulations. Moreover, our results underlined the importance of multiple biomarker species-specific analysis in trophic ecology studies, especially in complex and dynamic environments offering numerous food items to consumers

Mascart Thibaud - One of the best experts on this subject based on the ideXlab platform.

  • Trophic diversity of seagrass detritus copepods: A consequence of species-specific specialization or a random diet?
    'PeerJ', 2016
    Co-Authors: Mascart Thibaud, De Troch Marleen, Remy François, Michel Loïc, Lepoint Gilles
    Abstract:

    audience: researcher, professional, studentOne of the major ecological research questions is understanding how biodiversity influences ecosystem functioning. Unravelling interspecific feeding preferences of organisms with overlapping trophic niches will give part of the answer. Subsequently, the present study displays the trophic diversity of a benthic copepod community in a North-Western Corsican Posidonia oceanica seagrass ecosystem. These seagrass meadows are often interrupted by bare sand patches serving as deposition area for loose detritus. The accumulated macrophytodetritus, mainly derived from senescent macrophytes, harbour a diverse community of Harpacticoida (Crustacea, Copepoda). The most abundant copepods (i.e. three harpacticoids and one calanoid, belonging to different eco-morphological types) and their potential food sources (i.e. macrophytodetritus, epiphytic biofilm and suspended organic matter) were analysed for stable isotope ratios (δ13C, δ15N) and total lipids content. The results revealed a harpacticoid copepod feeding preference towards the epiphytic biofilm, while calanoid copepods preferred suspended organic matter. Additionally, a species-specific composition variation revealed finer partitioning of food resources (e.g. different micro-organisms present in the biofilm like bacteria, diatoms, fungi) over time.In conclusion, results showed species-specific food preferences, resulting in trophic niche and resource partitioning. Every eco-morphological type seems to cope in different ways with temporal fluctuations of food sources to comply with their nutritional needs. This illustrates the high resilience of the copepod community present in macrophytodetritus accumulations. Moreover, our results underlined the importance of multiple biomarker species-specific analysis in trophic ecology studies, especially in complex and dynamic environments offering numerous food items to consumers

  • Trophic diversity of seagrass detritus copepods: A consequence of species-specific specialization or a random diet?
    2016
    Co-Authors: Mascart Thibaud, De Troch Marleen, Remy François, Michel Loïc, Lepoint Gilles
    Abstract:

    One of the major ecological research questions is understanding how biodiversity influences ecosystem functioning. Unravelling interspecific feeding preferences of organisms with overlapping trophic niches will give part of the answer. Subsequently, the present study displays the trophic diversity of a benthic copepod community in a North-Western Corsican Posidonia oceanica seagrass ecosystem. These seagrass meadows are often interrupted by bare sand patches serving as deposition area for loose detritus. The accumulated macrophytodetritus, mainly derived from senescent macrophytes, harbour a diverse community of Harpacticoida (Crustacea, Copepoda). The most abundant copepods (i.e. three harpacticoids and one calanoid, belonging to different eco-morphological types) and their potential food sources (i.e. macrophytodetritus, epiphytic biofilm and suspended organic matter) were analysed for stable isotope ratios (δ13C, δ15N) and total lipids content. The results revealed a harpacticoid copepod feeding preference towards the epiphytic biofilm, while calanoid copepods preferred suspended organic matter. Additionally, a species-specific composition variation revealed finer partitioning of food resources (e.g. different micro-organisms present in the biofilm like bacteria, diatoms, fungi) over time.In conclusion, results showed species-specific food preferences, resulting in trophic niche and resource partitioning. Every eco-morphological type seems to cope in different ways with temporal fluctuations of food sources to comply with their nutritional needs. This illustrates the high resilience of the copepod community present in macrophytodetritus accumulations. Moreover, our results underlined the importance of multiple biomarker species-specific analysis in trophic ecology studies, especially in complex and dynamic environments offering numerous food items to consumers

Senjie Lin - One of the best experts on this subject based on the ideXlab platform.

  • Spliced leader RNA trans-splicing discovered in copepods.
    Scientific reports, 2015
    Co-Authors: Feifei Yang, Senjie Lin, Yunyun Zhuang, Yousong Huang, Hongju Chen, David A. Campbell, Nancy R. Sturm, Guangxing Liu
    Abstract:

    Copepods are one of the most abundant metazoans in the marine ecosystem, constituting a critical link in aquatic food webs and contributing significantly to the global carbon budget, yet molecular mechanisms of their gene expression are not well understood. Here we report the detection of spliced leader (SL) trans-splicing in calanoid copepods. We have examined nine species of wild-caught copepods from Jiaozhou Bay, China that represent the major families of the calanoids. All these species contained a common 46-nt SL (CopepodSL). We further determined the size of CopepodSL precursor RNA (slRNA; 108-158 nt) through genomic analysis and 3′-RACE technique, which was confirmed by RNA blot analysis. Structure modeling showed that the copepod slRNA folded into typical slRNA secondary structures. Using a CopepodSL-based primer set, we selectively enriched and sequenced copepod full-length cDNAs, which led to the characterization of copepod transcripts and the cataloging of the complete set of 79 eukaryotic cytoplasmic ribosomal proteins (cRPs) for a single copepod species. We uncovered the SL trans-splicing in copepod natural populations, and demonstrated that CopepodSL was a sensitive and specific tool for copepod transcriptomic studies at both the individual and population levels and that it would be useful for metatranscriptomic analysis of copepods.

  • Detecting In Situ Copepod Diet Diversity Using Molecular Technique: Development of a Copepod/Symbiotic Ciliate-Excluding Eukaryote-Inclusive PCR Protocol
    PloS one, 2014
    Co-Authors: Zhiling Guo, Edward J. Carpenter, Sheng Liu, Senjie Lin
    Abstract:

    Knowledge of in situ copepod diet diversity is crucial for accurately describing pelagic food web structure but is challenging to achieve due to lack of an easily applicable methodology. To enable analysis with whole copepod-derived DNAs, we developed a copepod-excluding 18S rDNA-based PCR protocol. Although it is effective in depressing amplification of copepod 18S rDNA, its applicability to detect diverse eukaryotes in both mono- and mixed-species has not been demonstrated. Besides, the protocol suffers from the problem that sequences from symbiotic ciliates are overrepresented in the retrieved 18S rDNA libraries. In this study, we designed a blocking primer to make a combined primer set (copepod/symbiotic ciliate-excluding eukaryote-common: CEEC) to depress PCR amplification of symbiotic ciliate sequences while maximizing the range of eukaryotes amplified. We firstly examined the specificity and efficacy of CEEC by PCR-amplifying DNAs from 16 copepod species, 37 representative organisms that are potential prey of copepods and a natural microplankton sample, and then evaluated the efficiency in reconstructing diet composition by detecting the food of both lab-reared and field-collected copepods. Our results showed that the CEEC primer set can successfully amplify 18S rDNA from a wide range of isolated species and mixed-species samples while depressing amplification of that from copepod and targeted symbiotic ciliate, indicating the universality of CEEC in specifically detecting prey of copepods. All the predetermined food offered to copepods in the laboratory were successfully retrieved, suggesting that the CEEC-based protocol can accurately reconstruct the diets of copepods without interference of copepods and their associated ciliates present in the DNA samples. Our initial application to analyzing the food composition of field-collected copepods uncovered diverse prey species, including those currently known, and those that are unsuspected, as copepod prey. While testing is required, this protocol provides a useful strategy for depicting in situ dietary composition of copepods.

  • detecting in situ copepod diet diversity using molecular technique development of a copepod symbiotic ciliate excluding eukaryote inclusive pcr protocol
    PLOS ONE, 2014
    Co-Authors: Zhiling Guo, Edward J. Carpenter, Sheng Liu, Senjie Lin
    Abstract:

    Knowledge of in situ copepod diet diversity is crucial for accurately describing pelagic food web structure but is challenging to achieve due to lack of an easily applicable methodology. To enable analysis with whole copepod-derived DNAs, we developed a copepod-excluding 18S rDNA-based PCR protocol. Although it is effective in depressing amplification of copepod 18S rDNA, its applicability to detect diverse eukaryotes in both mono- and mixed-species has not been demonstrated. Besides, the protocol suffers from the problem that sequences from symbiotic ciliates are overrepresented in the retrieved 18S rDNA libraries. In this study, we designed a blocking primer to make a combined primer set (copepod/symbiotic ciliate-excluding eukaryote-common: CEEC) to depress PCR amplification of symbiotic ciliate sequences while maximizing the range of eukaryotes amplified. We firstly examined the specificity and efficacy of CEEC by PCR-amplifying DNAs from 16 copepod species, 37 representative organisms that are potential prey of copepods and a natural microplankton sample, and then evaluated the efficiency in reconstructing diet composition by detecting the food of both lab-reared and field-collected copepods. Our results showed that the CEEC primer set can successfully amplify 18S rDNA from a wide range of isolated species and mixed-species samples while depressing amplification of that from copepod and targeted symbiotic ciliate, indicating the universality of CEEC in specifically detecting prey of copepods. All the predetermined food offered to copepods in the laboratory were successfully retrieved, suggesting that the CEEC-based protocol can accurately reconstruct the diets of copepods without interference of copepods and their associated ciliates present in the DNA samples. Our initial application to analyzing the food composition of field-collected copepods uncovered diverse prey species, including those currently known, and those that are unsuspected, as copepod prey. While testing is required, this protocol provides a useful strategy for depicting in situ dietary composition of copepods.

Remy François - One of the best experts on this subject based on the ideXlab platform.

  • Trophic diversity of seagrass detritus copepods: A consequence of species-specific specialization or a random diet?
    'PeerJ', 2016
    Co-Authors: Mascart Thibaud, De Troch Marleen, Remy François, Michel Loïc, Lepoint Gilles
    Abstract:

    audience: researcher, professional, studentOne of the major ecological research questions is understanding how biodiversity influences ecosystem functioning. Unravelling interspecific feeding preferences of organisms with overlapping trophic niches will give part of the answer. Subsequently, the present study displays the trophic diversity of a benthic copepod community in a North-Western Corsican Posidonia oceanica seagrass ecosystem. These seagrass meadows are often interrupted by bare sand patches serving as deposition area for loose detritus. The accumulated macrophytodetritus, mainly derived from senescent macrophytes, harbour a diverse community of Harpacticoida (Crustacea, Copepoda). The most abundant copepods (i.e. three harpacticoids and one calanoid, belonging to different eco-morphological types) and their potential food sources (i.e. macrophytodetritus, epiphytic biofilm and suspended organic matter) were analysed for stable isotope ratios (δ13C, δ15N) and total lipids content. The results revealed a harpacticoid copepod feeding preference towards the epiphytic biofilm, while calanoid copepods preferred suspended organic matter. Additionally, a species-specific composition variation revealed finer partitioning of food resources (e.g. different micro-organisms present in the biofilm like bacteria, diatoms, fungi) over time.In conclusion, results showed species-specific food preferences, resulting in trophic niche and resource partitioning. Every eco-morphological type seems to cope in different ways with temporal fluctuations of food sources to comply with their nutritional needs. This illustrates the high resilience of the copepod community present in macrophytodetritus accumulations. Moreover, our results underlined the importance of multiple biomarker species-specific analysis in trophic ecology studies, especially in complex and dynamic environments offering numerous food items to consumers

  • Trophic diversity of seagrass detritus copepods: A consequence of species-specific specialization or a random diet?
    2016
    Co-Authors: Mascart Thibaud, De Troch Marleen, Remy François, Michel Loïc, Lepoint Gilles
    Abstract:

    One of the major ecological research questions is understanding how biodiversity influences ecosystem functioning. Unravelling interspecific feeding preferences of organisms with overlapping trophic niches will give part of the answer. Subsequently, the present study displays the trophic diversity of a benthic copepod community in a North-Western Corsican Posidonia oceanica seagrass ecosystem. These seagrass meadows are often interrupted by bare sand patches serving as deposition area for loose detritus. The accumulated macrophytodetritus, mainly derived from senescent macrophytes, harbour a diverse community of Harpacticoida (Crustacea, Copepoda). The most abundant copepods (i.e. three harpacticoids and one calanoid, belonging to different eco-morphological types) and their potential food sources (i.e. macrophytodetritus, epiphytic biofilm and suspended organic matter) were analysed for stable isotope ratios (δ13C, δ15N) and total lipids content. The results revealed a harpacticoid copepod feeding preference towards the epiphytic biofilm, while calanoid copepods preferred suspended organic matter. Additionally, a species-specific composition variation revealed finer partitioning of food resources (e.g. different micro-organisms present in the biofilm like bacteria, diatoms, fungi) over time.In conclusion, results showed species-specific food preferences, resulting in trophic niche and resource partitioning. Every eco-morphological type seems to cope in different ways with temporal fluctuations of food sources to comply with their nutritional needs. This illustrates the high resilience of the copepod community present in macrophytodetritus accumulations. Moreover, our results underlined the importance of multiple biomarker species-specific analysis in trophic ecology studies, especially in complex and dynamic environments offering numerous food items to consumers

De Troch Marleen - One of the best experts on this subject based on the ideXlab platform.

  • Trophic diversity of seagrass detritus copepods: A consequence of species-specific specialization or a random diet?
    'PeerJ', 2016
    Co-Authors: Mascart Thibaud, De Troch Marleen, Remy François, Michel Loïc, Lepoint Gilles
    Abstract:

    audience: researcher, professional, studentOne of the major ecological research questions is understanding how biodiversity influences ecosystem functioning. Unravelling interspecific feeding preferences of organisms with overlapping trophic niches will give part of the answer. Subsequently, the present study displays the trophic diversity of a benthic copepod community in a North-Western Corsican Posidonia oceanica seagrass ecosystem. These seagrass meadows are often interrupted by bare sand patches serving as deposition area for loose detritus. The accumulated macrophytodetritus, mainly derived from senescent macrophytes, harbour a diverse community of Harpacticoida (Crustacea, Copepoda). The most abundant copepods (i.e. three harpacticoids and one calanoid, belonging to different eco-morphological types) and their potential food sources (i.e. macrophytodetritus, epiphytic biofilm and suspended organic matter) were analysed for stable isotope ratios (δ13C, δ15N) and total lipids content. The results revealed a harpacticoid copepod feeding preference towards the epiphytic biofilm, while calanoid copepods preferred suspended organic matter. Additionally, a species-specific composition variation revealed finer partitioning of food resources (e.g. different micro-organisms present in the biofilm like bacteria, diatoms, fungi) over time.In conclusion, results showed species-specific food preferences, resulting in trophic niche and resource partitioning. Every eco-morphological type seems to cope in different ways with temporal fluctuations of food sources to comply with their nutritional needs. This illustrates the high resilience of the copepod community present in macrophytodetritus accumulations. Moreover, our results underlined the importance of multiple biomarker species-specific analysis in trophic ecology studies, especially in complex and dynamic environments offering numerous food items to consumers

  • Trophic diversity of seagrass detritus copepods: A consequence of species-specific specialization or a random diet?
    2016
    Co-Authors: Mascart Thibaud, De Troch Marleen, Remy François, Michel Loïc, Lepoint Gilles
    Abstract:

    One of the major ecological research questions is understanding how biodiversity influences ecosystem functioning. Unravelling interspecific feeding preferences of organisms with overlapping trophic niches will give part of the answer. Subsequently, the present study displays the trophic diversity of a benthic copepod community in a North-Western Corsican Posidonia oceanica seagrass ecosystem. These seagrass meadows are often interrupted by bare sand patches serving as deposition area for loose detritus. The accumulated macrophytodetritus, mainly derived from senescent macrophytes, harbour a diverse community of Harpacticoida (Crustacea, Copepoda). The most abundant copepods (i.e. three harpacticoids and one calanoid, belonging to different eco-morphological types) and their potential food sources (i.e. macrophytodetritus, epiphytic biofilm and suspended organic matter) were analysed for stable isotope ratios (δ13C, δ15N) and total lipids content. The results revealed a harpacticoid copepod feeding preference towards the epiphytic biofilm, while calanoid copepods preferred suspended organic matter. Additionally, a species-specific composition variation revealed finer partitioning of food resources (e.g. different micro-organisms present in the biofilm like bacteria, diatoms, fungi) over time.In conclusion, results showed species-specific food preferences, resulting in trophic niche and resource partitioning. Every eco-morphological type seems to cope in different ways with temporal fluctuations of food sources to comply with their nutritional needs. This illustrates the high resilience of the copepod community present in macrophytodetritus accumulations. Moreover, our results underlined the importance of multiple biomarker species-specific analysis in trophic ecology studies, especially in complex and dynamic environments offering numerous food items to consumers