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Juan J. Calvete - One of the best experts on this subject based on the ideXlab platform.
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Venomic Analysis of the Poorly Studied Desert Coral Snake, Micrurus tschudii tschudii, Supports the 3FTx/PLA2 Dichotomy across Micrurus Venoms
Toxins, 2016Co-Authors: Libia Sanz, Davinia Pla, Bruno Lomonte, María Salas, Alfonso Zavaleta, Alicia Perez, Yania Rodriguez, Juan J. CalveteAbstract:The venom proteome of the poorly studied desert Coral Snake Micrurus tschudii tschudii was unveiled using a venomic approach, which identified ≥38 proteins belonging to only four Snake venom protein families. The three-finger toxins (3FTxs) constitute, both in number of isoforms (~30) and total abundance (93.6% of the venom proteome), the major protein family of the desert Coral Snake venom. Phospholipases A2 (PLA2s; seven isoforms, 4.1% of the venom proteome), 1–3 Kunitz-type proteins (1.6%), and 1–2 l-amino acid oxidases (LAO, 0.7%) complete the toxin arsenal of M. t. tschudii. Our results add to the growing evidence that the occurrence of two divergent venom phenotypes, i.e., 3FTx- and PLA2-predominant venom proteomes, may constitute a general trend across the cladogenesis of Micrurus. The occurrence of a similar pattern of venom phenotypic variability among true sea Snake (Hydrophiinae) venoms suggests that the 3FTx/PLA2 dichotomy may be widely distributed among Elapidae venoms.
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venomic analysis of the poorly studied desert Coral Snake micrurus tschudii tschudii supports the 3ftx pla2 dichotomy across micrurus venoms
Toxins, 2016Co-Authors: Libia Sanz, Davinia Pla, Bruno Lomonte, María Salas, Alfonso Zavaleta, Alicia Perez, Yania Rodriguez, Juan J. CalveteAbstract:The venom proteome of the poorly studied desert Coral Snake Micrurus tschudii tschudii was unveiled using a venomic approach, which identified ≥38 proteins belonging to only four Snake venom protein families. The three-finger toxins (3FTxs) constitute, both in number of isoforms (~30) and total abundance (93.6% of the venom proteome), the major protein family of the desert Coral Snake venom. Phospholipases A2 (PLA2s; seven isoforms, 4.1% of the venom proteome), 1–3 Kunitz-type proteins (1.6%), and 1–2 l-amino acid oxidases (LAO, 0.7%) complete the toxin arsenal of M. t. tschudii. Our results add to the growing evidence that the occurrence of two divergent venom phenotypes, i.e., 3FTx- and PLA2-predominant venom proteomes, may constitute a general trend across the cladogenesis of Micrurus. The occurrence of a similar pattern of venom phenotypic variability among true sea Snake (Hydrophiinae) venoms suggests that the 3FTx/PLA2 dichotomy may be widely distributed among Elapidae venoms.
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venomic and antivenomic analyses of the central american Coral Snake micrurus nigrocinctus elapidae
Journal of Proteome Research, 2011Co-Authors: Julián Fernández, Libia Sanz, José María Gutiérrez, Juan J. Calvete, Alberto Alapegiron, Yamileth Angulo, Bruno LomonteAbstract:The proteome of the venom of Micrurus nigrocinctus (Central American Coral Snake) was analyzed by a “venomics” approach. Nearly 50 venom peaks were resolved by RP-HPLC, revealing a complex protein ...
Bruno Lomonte - One of the best experts on this subject based on the ideXlab platform.
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functional proteomic and transcriptomic characterization of the venom from micrurus browni browni identification of the first lethal multimeric neurotoxin in Coral Snake venom
Journal of Proteomics, 2020Co-Authors: Melisa Benardvalle, Bruno Lomonte, Edgar Nericastro, Manuel F Yanezmendoza, Alejandro Olvera, Fernando Z Zamudio, Rita Restanocassulini, Lourival D Possani, Enrique JimenezferrerAbstract:Abstract Proteomic characterization of Micrurus browni browni venom showed approximately 41 components belonging to 9 protein families, mainly phospholipases A2 (PLA2s) and three-finger toxins (3FTxs). Venom gland transcriptome yielded 39 venom transcripts belonging to 10 protein families. Functional characterization identified a multimeric toxin, here designated Brownitoxin-1, which comprises at least one PLA2 and one 3FTx. Its components have no or very low lethality individually but become extremely lethal when combined; both were partially characterized. Other two lethal components were identified: A neurotoxic PLA2, and a postsynaptic α-neurotoxin. LD50s as well as PLA2 and nAChR-blocking activities were determined for whole venom and isolated components. Application of venom to murine neuromuscular preparations caused a progressive decrease of twitch force that was irreversible after washing. Inhibition of PLA2 activity with p-bromophenacyl bromide (pBPB) showed that approximately 90% of toxicity is dependent on this activity. Non-lethal components include diverse 3FTxs, at least three enzymatically active PLA2s and the nociceptive toxin MitTx. No evidence of specificity towards prey was observed. This work is one of the most complete characterizations of a Coral Snake venom so far and its findings highlight the relevance of protein complexes in venom function. Significance This study represents a profound analysis of the venom of the Coral Snake Micrurus browni browni, including a venom proteome, venom gland transcriptomic data and functional studies of whole venom and isolated toxins. It significantly contributes to the understanding of North American Coral Snake venoms, which are currently largely unknown. It includes characterization of relevant venom components, one of which represents the first description of a lethal multimeric neurotoxin in Coral Snake venom. This work highlights the importance of protein complexes in Coral Snake venom and could serve as a basis for the finding of several other multimeric toxins. Finally, we report the absence of taxon specificity, which has been previously reported in the venoms of other Snakes of the same genus.
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venomic analysis of the poorly studied desert Coral Snake micrurus tschudii tschudii supports the 3ftx pla2 dichotomy across micrurus venoms
Toxins, 2016Co-Authors: Libia Sanz, Davinia Pla, Bruno Lomonte, María Salas, Alfonso Zavaleta, Alicia Perez, Yania Rodriguez, Juan J. CalveteAbstract:The venom proteome of the poorly studied desert Coral Snake Micrurus tschudii tschudii was unveiled using a venomic approach, which identified ≥38 proteins belonging to only four Snake venom protein families. The three-finger toxins (3FTxs) constitute, both in number of isoforms (~30) and total abundance (93.6% of the venom proteome), the major protein family of the desert Coral Snake venom. Phospholipases A2 (PLA2s; seven isoforms, 4.1% of the venom proteome), 1–3 Kunitz-type proteins (1.6%), and 1–2 l-amino acid oxidases (LAO, 0.7%) complete the toxin arsenal of M. t. tschudii. Our results add to the growing evidence that the occurrence of two divergent venom phenotypes, i.e., 3FTx- and PLA2-predominant venom proteomes, may constitute a general trend across the cladogenesis of Micrurus. The occurrence of a similar pattern of venom phenotypic variability among true sea Snake (Hydrophiinae) venoms suggests that the 3FTx/PLA2 dichotomy may be widely distributed among Elapidae venoms.
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Venomic Analysis of the Poorly Studied Desert Coral Snake, Micrurus tschudii tschudii, Supports the 3FTx/PLA2 Dichotomy across Micrurus Venoms
Toxins, 2016Co-Authors: Libia Sanz, Davinia Pla, Bruno Lomonte, María Salas, Alfonso Zavaleta, Alicia Perez, Yania Rodriguez, Juan J. CalveteAbstract:The venom proteome of the poorly studied desert Coral Snake Micrurus tschudii tschudii was unveiled using a venomic approach, which identified ≥38 proteins belonging to only four Snake venom protein families. The three-finger toxins (3FTxs) constitute, both in number of isoforms (~30) and total abundance (93.6% of the venom proteome), the major protein family of the desert Coral Snake venom. Phospholipases A2 (PLA2s; seven isoforms, 4.1% of the venom proteome), 1–3 Kunitz-type proteins (1.6%), and 1–2 l-amino acid oxidases (LAO, 0.7%) complete the toxin arsenal of M. t. tschudii. Our results add to the growing evidence that the occurrence of two divergent venom phenotypes, i.e., 3FTx- and PLA2-predominant venom proteomes, may constitute a general trend across the cladogenesis of Micrurus. The occurrence of a similar pattern of venom phenotypic variability among true sea Snake (Hydrophiinae) venoms suggests that the 3FTx/PLA2 dichotomy may be widely distributed among Elapidae venoms.
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integrative characterization of the venom of the Coral Snake micrurus dumerilii elapidae from colombia proteome toxicity and cross neutralization by antivenom
Journal of Proteomics, 2016Co-Authors: Paola Reysuarez, Julián Fernández, Vitelbina Núñez, Bruno LomonteAbstract:Abstract In Colombia, nearly 2.8% of the 4200 Snakebite accidents recorded annually are inflicted by Coral Snakes (genus Micrurus ). Micrurus dumerilii has a broad distribution in this country, especially in densely populated areas. The proteomic profile of its venom was here studied by a bottom-up approach combining RP-HPLC, SDS-PAGE and MALDI-TOF/TOF. Venom proteins were assigned to eleven families, the most abundant being phospholipases A 2 (PLA 2 ; 52.0%) and three-finger toxins (3FTx; 28.1%). This compositional profile shows that M. dumerilii venom belongs to the ‘PLA 2 -rich’ phenotype, in the recently proposed dichotomy for Micrurus venoms. Enzymatic and toxic venom activities correlated with protein family abundances. Whole venom induced a conspicuous myotoxic, cytotoxic and anticoagulant effect, and was mildly edematogenic and proteolytic, whereas it lacked hemorrhagic activity. Some 3FTxs and PLA 2 s reproduced the lethal effect of venom. A Coral Snake antivenom to Micrurus nigrocinctus demonstrated significant cross-recognition of M. dumerilii venom proteins, and accordingly, ability to neutralize its lethal effect. The combined compositional, functional, and immunological data here reported for M. dumerilii venom may contribute to a better understanding of these envenomings, and support the possible use of anti- M. nigrocinctus Coral Snake antivenom in their treatment. Biological significance Coral Snakes represent a highly diversified group of elapids in the New World, with nearly 70 species within the genus Micrurus . Owing to their scarce yields, the biochemical composition and toxic activities of Coral Snake venoms have been less well characterized than those of viperid species. In this work, an integrative view of the venom of M. dumerilii , a medically relevant Coral Snake from Colombia, was obtained by a combined proteomic, functional, and immunological approach. The venom contains proteins from at least eleven families, with a predominance of phospholipases A 2 (PLA 2 ), followed by three-finger toxins (3FTx). According to its compositional profile, M. dumerilii venom can be grouped with those of several Micrurus species from North and Central America that present a PLA 2 -predominant phenotype, to date it is the most southerly Coral Snake species to do so. Other Coral Snake species that a ‘PLA 2 -rich’ venom, M. dumerilii venom contains both components that form MitTx, a pain-inducing heterodimeric complex recently characterized from the venom of Micrurus tener , also present in Micrurus mosquitensis and M. nigrocinctus venoms. In addition to a lethal three-finger toxin, PLA 2 s participate in the toxicity of M. dumerilii venom, some of them displaying ability to induce cytolysis, muscle necrosis, and lethality to mice. An antivenom to M. nigrocinctus demonstrated significant cross-recognition of M. dumerilii venom proteins, and accordingly, ability to neutralize its lethal effect, being of potential therapeutic usefulness in these envenomings.
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venomic and antivenomic analyses of the central american Coral Snake micrurus nigrocinctus elapidae
Journal of Proteome Research, 2011Co-Authors: Julián Fernández, Libia Sanz, José María Gutiérrez, Juan J. Calvete, Alberto Alapegiron, Yamileth Angulo, Bruno LomonteAbstract:The proteome of the venom of Micrurus nigrocinctus (Central American Coral Snake) was analyzed by a “venomics” approach. Nearly 50 venom peaks were resolved by RP-HPLC, revealing a complex protein ...
Libia Sanz - One of the best experts on this subject based on the ideXlab platform.
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Venomic Analysis of the Poorly Studied Desert Coral Snake, Micrurus tschudii tschudii, Supports the 3FTx/PLA2 Dichotomy across Micrurus Venoms
Toxins, 2016Co-Authors: Libia Sanz, Davinia Pla, Bruno Lomonte, María Salas, Alfonso Zavaleta, Alicia Perez, Yania Rodriguez, Juan J. CalveteAbstract:The venom proteome of the poorly studied desert Coral Snake Micrurus tschudii tschudii was unveiled using a venomic approach, which identified ≥38 proteins belonging to only four Snake venom protein families. The three-finger toxins (3FTxs) constitute, both in number of isoforms (~30) and total abundance (93.6% of the venom proteome), the major protein family of the desert Coral Snake venom. Phospholipases A2 (PLA2s; seven isoforms, 4.1% of the venom proteome), 1–3 Kunitz-type proteins (1.6%), and 1–2 l-amino acid oxidases (LAO, 0.7%) complete the toxin arsenal of M. t. tschudii. Our results add to the growing evidence that the occurrence of two divergent venom phenotypes, i.e., 3FTx- and PLA2-predominant venom proteomes, may constitute a general trend across the cladogenesis of Micrurus. The occurrence of a similar pattern of venom phenotypic variability among true sea Snake (Hydrophiinae) venoms suggests that the 3FTx/PLA2 dichotomy may be widely distributed among Elapidae venoms.
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venomic analysis of the poorly studied desert Coral Snake micrurus tschudii tschudii supports the 3ftx pla2 dichotomy across micrurus venoms
Toxins, 2016Co-Authors: Libia Sanz, Davinia Pla, Bruno Lomonte, María Salas, Alfonso Zavaleta, Alicia Perez, Yania Rodriguez, Juan J. CalveteAbstract:The venom proteome of the poorly studied desert Coral Snake Micrurus tschudii tschudii was unveiled using a venomic approach, which identified ≥38 proteins belonging to only four Snake venom protein families. The three-finger toxins (3FTxs) constitute, both in number of isoforms (~30) and total abundance (93.6% of the venom proteome), the major protein family of the desert Coral Snake venom. Phospholipases A2 (PLA2s; seven isoforms, 4.1% of the venom proteome), 1–3 Kunitz-type proteins (1.6%), and 1–2 l-amino acid oxidases (LAO, 0.7%) complete the toxin arsenal of M. t. tschudii. Our results add to the growing evidence that the occurrence of two divergent venom phenotypes, i.e., 3FTx- and PLA2-predominant venom proteomes, may constitute a general trend across the cladogenesis of Micrurus. The occurrence of a similar pattern of venom phenotypic variability among true sea Snake (Hydrophiinae) venoms suggests that the 3FTx/PLA2 dichotomy may be widely distributed among Elapidae venoms.
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venomic and antivenomic analyses of the central american Coral Snake micrurus nigrocinctus elapidae
Journal of Proteome Research, 2011Co-Authors: Julián Fernández, Libia Sanz, José María Gutiérrez, Juan J. Calvete, Alberto Alapegiron, Yamileth Angulo, Bruno LomonteAbstract:The proteome of the venom of Micrurus nigrocinctus (Central American Coral Snake) was analyzed by a “venomics” approach. Nearly 50 venom peaks were resolved by RP-HPLC, revealing a complex protein ...
Alicia Perez - One of the best experts on this subject based on the ideXlab platform.
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venomic analysis of the poorly studied desert Coral Snake micrurus tschudii tschudii supports the 3ftx pla2 dichotomy across micrurus venoms
Toxins, 2016Co-Authors: Libia Sanz, Davinia Pla, Bruno Lomonte, María Salas, Alfonso Zavaleta, Alicia Perez, Yania Rodriguez, Juan J. CalveteAbstract:The venom proteome of the poorly studied desert Coral Snake Micrurus tschudii tschudii was unveiled using a venomic approach, which identified ≥38 proteins belonging to only four Snake venom protein families. The three-finger toxins (3FTxs) constitute, both in number of isoforms (~30) and total abundance (93.6% of the venom proteome), the major protein family of the desert Coral Snake venom. Phospholipases A2 (PLA2s; seven isoforms, 4.1% of the venom proteome), 1–3 Kunitz-type proteins (1.6%), and 1–2 l-amino acid oxidases (LAO, 0.7%) complete the toxin arsenal of M. t. tschudii. Our results add to the growing evidence that the occurrence of two divergent venom phenotypes, i.e., 3FTx- and PLA2-predominant venom proteomes, may constitute a general trend across the cladogenesis of Micrurus. The occurrence of a similar pattern of venom phenotypic variability among true sea Snake (Hydrophiinae) venoms suggests that the 3FTx/PLA2 dichotomy may be widely distributed among Elapidae venoms.
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Venomic Analysis of the Poorly Studied Desert Coral Snake, Micrurus tschudii tschudii, Supports the 3FTx/PLA2 Dichotomy across Micrurus Venoms
Toxins, 2016Co-Authors: Libia Sanz, Davinia Pla, Bruno Lomonte, María Salas, Alfonso Zavaleta, Alicia Perez, Yania Rodriguez, Juan J. CalveteAbstract:The venom proteome of the poorly studied desert Coral Snake Micrurus tschudii tschudii was unveiled using a venomic approach, which identified ≥38 proteins belonging to only four Snake venom protein families. The three-finger toxins (3FTxs) constitute, both in number of isoforms (~30) and total abundance (93.6% of the venom proteome), the major protein family of the desert Coral Snake venom. Phospholipases A2 (PLA2s; seven isoforms, 4.1% of the venom proteome), 1–3 Kunitz-type proteins (1.6%), and 1–2 l-amino acid oxidases (LAO, 0.7%) complete the toxin arsenal of M. t. tschudii. Our results add to the growing evidence that the occurrence of two divergent venom phenotypes, i.e., 3FTx- and PLA2-predominant venom proteomes, may constitute a general trend across the cladogenesis of Micrurus. The occurrence of a similar pattern of venom phenotypic variability among true sea Snake (Hydrophiinae) venoms suggests that the 3FTx/PLA2 dichotomy may be widely distributed among Elapidae venoms.
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Neutralization of Coral Snake Micrurus nigrocinctus venom by a monovalent antivenom
Brazilian journal of medical and biological research = Revista brasileira de pesquisas medicas e biologicas, 1991Co-Authors: José María Gutiérrez, Alicia Perez, Gustavo Rojas, Argüello I, Bruno LomonteAbstract:1. The neutralizing ability of a monovalent anti-Micrurus nigrocinctus (Coral Snake) antivenom produced in Costa Rica was tested against the lethal, myotoxic and phospholipase A2 activities of homologous venom. In addition, immunodiffusion and Western blot analyses were performed. 2. In experiments where venom and antivenom were incubated prior to the test, antivenom was effective in neutralizing lethal, myotoxic and phospholipase A2 activities, with Effective Doses 50% of 2700 microliters antivenom/mg venom, 1840 microliters antivenom/mg venom, and 3630 microliters antivenom/mg venom, respectively. 3. When Coral Snake antivenom was administered at different times after Coral Snake venom injection, neutralization of lethality was achieved when antivenom was injected i.v. immediately and 15 min after venom. In contrast, lethality was not reduced when antivenom was administered by the im route. Only partial neutralization of myotoxicity was observed even when antivenom was injected i.v. immediately after envenomation. 4. Immunodiffusion and immunoblot analyses demonstrated the presence of antibodies in antivenom against several, but not all, venom components.
Davinia Pla - One of the best experts on this subject based on the ideXlab platform.
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venomic analysis of the poorly studied desert Coral Snake micrurus tschudii tschudii supports the 3ftx pla2 dichotomy across micrurus venoms
Toxins, 2016Co-Authors: Libia Sanz, Davinia Pla, Bruno Lomonte, María Salas, Alfonso Zavaleta, Alicia Perez, Yania Rodriguez, Juan J. CalveteAbstract:The venom proteome of the poorly studied desert Coral Snake Micrurus tschudii tschudii was unveiled using a venomic approach, which identified ≥38 proteins belonging to only four Snake venom protein families. The three-finger toxins (3FTxs) constitute, both in number of isoforms (~30) and total abundance (93.6% of the venom proteome), the major protein family of the desert Coral Snake venom. Phospholipases A2 (PLA2s; seven isoforms, 4.1% of the venom proteome), 1–3 Kunitz-type proteins (1.6%), and 1–2 l-amino acid oxidases (LAO, 0.7%) complete the toxin arsenal of M. t. tschudii. Our results add to the growing evidence that the occurrence of two divergent venom phenotypes, i.e., 3FTx- and PLA2-predominant venom proteomes, may constitute a general trend across the cladogenesis of Micrurus. The occurrence of a similar pattern of venom phenotypic variability among true sea Snake (Hydrophiinae) venoms suggests that the 3FTx/PLA2 dichotomy may be widely distributed among Elapidae venoms.
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Venomic Analysis of the Poorly Studied Desert Coral Snake, Micrurus tschudii tschudii, Supports the 3FTx/PLA2 Dichotomy across Micrurus Venoms
Toxins, 2016Co-Authors: Libia Sanz, Davinia Pla, Bruno Lomonte, María Salas, Alfonso Zavaleta, Alicia Perez, Yania Rodriguez, Juan J. CalveteAbstract:The venom proteome of the poorly studied desert Coral Snake Micrurus tschudii tschudii was unveiled using a venomic approach, which identified ≥38 proteins belonging to only four Snake venom protein families. The three-finger toxins (3FTxs) constitute, both in number of isoforms (~30) and total abundance (93.6% of the venom proteome), the major protein family of the desert Coral Snake venom. Phospholipases A2 (PLA2s; seven isoforms, 4.1% of the venom proteome), 1–3 Kunitz-type proteins (1.6%), and 1–2 l-amino acid oxidases (LAO, 0.7%) complete the toxin arsenal of M. t. tschudii. Our results add to the growing evidence that the occurrence of two divergent venom phenotypes, i.e., 3FTx- and PLA2-predominant venom proteomes, may constitute a general trend across the cladogenesis of Micrurus. The occurrence of a similar pattern of venom phenotypic variability among true sea Snake (Hydrophiinae) venoms suggests that the 3FTx/PLA2 dichotomy may be widely distributed among Elapidae venoms.