The Experts below are selected from a list of 4488 Experts worldwide ranked by ideXlab platform
Xue Xiao - One of the best experts on this subject based on the ideXlab platform.
-
classification and analysis of Corn Steep Liquor by uplc q tof ms and hplc
Talanta, 2013Co-Authors: Xue Xiao, Jun Du, Hongzhi Zhao, Linyi Dong, Yiming WangAbstract:Abstract Corn Steep Liquor (CSL), an important raw material with high nutritional value, serves as a nitrogen source in the fermentation industry. The CSL quality directly affects the yield and quality of fermentation products. In this work, a fingerprinting technique was used to identify the potential markers of CSL. Forty-two CSL samples from different manufacturers were profiled by ultra-performance liquid chromatography with tandem quadrupole time-of-flight mass spectrometry. Sixteen compounds, almost all of which were amino acids and their derivatives, were considered as the potential markers. Then, o -phthalaldehyde-9-fluorenylmethyl chloroformate precolumn derivatization by high-performance liquid chromatography was performed to identify the free amino acids in CSL. Principal component analysis (PCA) was used to distinguish among the samples from different manufacturers. The results demonstrated that the fingerprinting technique combined with PCA analysis was a powerful tool for determining the CSL quality.
-
Classification and analysis of Corn Steep Liquor by UPLC/Q-TOF MS and HPLC
Talanta, 2013Co-Authors: Xue Xiao, Jun Du, Hongzhi Zhao, Linyi Dong, Yiming WangAbstract:Abstract Corn Steep Liquor (CSL), an important raw material with high nutritional value, serves as a nitrogen source in the fermentation industry. The CSL quality directly affects the yield and quality of fermentation products. In this work, a fingerprinting technique was used to identify the potential markers of CSL. Forty-two CSL samples from different manufacturers were profiled by ultra-performance liquid chromatography with tandem quadrupole time-of-flight mass spectrometry. Sixteen compounds, almost all of which were amino acids and their derivatives, were considered as the potential markers. Then, o -phthalaldehyde-9-fluorenylmethyl chloroformate precolumn derivatization by high-performance liquid chromatography was performed to identify the free amino acids in CSL. Principal component analysis (PCA) was used to distinguish among the samples from different manufacturers. The results demonstrated that the fingerprinting technique combined with PCA analysis was a powerful tool for determining the CSL quality.
-
determination of vitamins b2 b3 b6 and b7 in Corn Steep Liquor by nir and plsr
Transactions of Tianjin University, 2012Co-Authors: Xue Xiao, Jun DuAbstract:Corn Steep Liquor (CSL) is an important raw material that has high nutritional value and serves as a nitrogen source. Biotin in CSL is especially of great importance to fermentation. In order to develop a fast, versatile, cheap, and environmentally safe analytical method for quantifying vitamins B2 (VB2), B3 (VB3), B6 (VB6) and B7 (VB7) in CSL, the near-infrared spectroscopy (NIR) measurements of 66 samples (22 batches) of CSL were analyzed by partial least-square regression (PLSR). Multivariate models developed in the NIR regions showed good predictive abilities for VB2, VB3, VB6 and VB7. Results confirmed the probability of the multivariate spectroscopic approach as a replacement for expensive and time-consuming conventional chemical methods.
-
Determination of Vitamins B2, B3, B6 and B7 in Corn Steep Liquor by NIR and PLSR *
Transactions of Tianjin University, 2012Co-Authors: Xue Xiao, Jun DuAbstract:Corn Steep Liquor (CSL) is an important raw material that has high nutritional value and serves as a nitrogen source. Biotin in CSL is especially of great importance to fermentation. In order to develop a fast, versatile, cheap, and environmentally safe analytical method for quantifying vitamins B2 (VB2), B3 (VB3), B6 (VB6) and B7 (VB7) in CSL, the near-infrared spectroscopy (NIR) measurements of 66 samples (22 batches) of CSL were analyzed by partial least-square regression (PLSR). Multivariate models developed in the NIR regions showed good predictive abilities for VB2, VB3, VB6 and VB7. Results confirmed the probability of the multivariate spectroscopic approach as a replacement for expensive and time-consuming conventional chemical methods.
A B Moldes - One of the best experts on this subject based on the ideXlab platform.
-
ionic behavior assessment of surface active compounds from Corn Steep Liquor by exchange resins
Journal of Surfactants and Detergents, 2017Co-Authors: L Rodriguezlopez, M Rinconfontan, X Vecino, J M Cruz, A B MoldesAbstract:Depending on their ionic nature, biosurfactants can be classified as nonionic, anionic, cationic, or amphoteric. The ionic behavior of biosurfactants is an important characteristic that dictates their use in industrial applications. In this work, a biosurfactant extract obtained from Corn Steep Liquor was subjected to anionic or cationic resins, in order to study the ionic behavior under different operational conditions using response surface methodology. The independent variables included in the study are the dilution of biosurfactant solution, the amount of cationic or anionic resin, and the extraction time, whereas the dependent variables studied consisted of the surface tension of biosurfactant aqueous solution, after contacting with anionic or cationic resin. The results showed that biosurfactant extracted from Corn Steep Liquor is amphoteric, since both resins were able to entrap this biosurfactant, making it particularly suited for use in personal care preparations for sensitive skin.
-
a multifunctional extract from Corn Steep Liquor antioxidant and surfactant activities
Food & Function, 2016Co-Authors: L Rodriguezlopez, X Vecino, A B Moldes, Letricia Barbosapereira, J M CruzAbstract:In the last few years the awareness of consumers and institutions about the impact that our industrial processes has on health and the environment has increased, demanding more natural products. In this work, a multifunctional bioactive extract with surfactant and antioxidant properties, composed mainly of C16 and C18 fatty acids, and phenolic compounds (vanillic acid, p-coumaric acid, ferulic acid, sinapic acid and quercetin) was obtained from Corn Steep Liquor (CSL). Different liquid–liquid extraction protocols were evaluated obtaining a natural extract, which was able to reduce the surface tension of water by more than 30 units, showing antioxidant activity with an EC50 of 8.51 mg mL−1 and a yield of 6.85 g of extract per kg of CSL. Additionally, it was observed that after liquid–liquid extraction with chloroform, the aqueous phase can be subjected to a hydrothermal treatment, followed by extraction with ethyl acetate, in order to obtain another extract (24.7 g of extract per kg of CSL) with a higher antioxidant capacity (EC50 of 4.02 mg mL−1). In this case the antioxidant extract is composed of protocatechuic acid, vanillic acid, caffeic acid, epicatechin, p-coumaric acid, ferulic acid, sinapic acid and quercetin.
-
Optimization of liquid–liquid extraction of biosurfactants from Corn Steep Liquor
Bioprocess and Biosystems Engineering, 2015Co-Authors: X Vecino, J M Cruz, L. Barbosa-pereira, R. Devesa-rey, A B MoldesAbstract:In this work, the optimization of the operational conditions for the chloroform-based extraction of surface-active compounds from Corn Steep Liquor (CSL) was carried out and the nutritional properties of the remnant aqueous phase (CSL-less biosurfactant) was evaluated as microbial fermentation medium. The optimal conditions to obtain biosurfactants from CSL were as follows: chloroform/CSL ratio 2 (v/v), 56 °C at extraction times >30 min. At the optima conditions, 100 % of biosurfactant extract can be obtained from CSL, obtaining 12.0 ± 0.5 g of biosurfactant extract/Kg of CSL. The critical micelle concentration (CMC) of the biosurfactant extract was 399.4 mg L^−1. This value is similar to the CMC of cetrimonium bromide (CTAB), a cationic surfactant used in the formulation of nanoparticles. The extraction of biosurfactant can be also carried out at room temperature although in this case, the extraction yield decreased about 15 %. The extraction of surface-active compounds from agroindustrial streams can suppose important advances for the bio-based surfactants industry. Biosurfactants obtained in this work are not only more eco-friendly than chemical detergents but also can be cost competitive with its chemical counterparts. Furthermore, after the extraction of surface-active compounds, CSL-less biosurfactant was found to be suitable as nutritional supplement for lactic acid bacteria, maintaining its nutritional properties in comparison with regular CSL.
-
sewage sludge polycyclic aromatic hydrocarbon pah decontamination technique based on the utilization of a lipopeptide biosurfactant extracted from Corn Steep Liquor
Journal of Agricultural and Food Chemistry, 2015Co-Authors: X Vecino, L Rodriguezlopez, J M Cruz, A B MoldesAbstract:A decontamination technique based on the utilization of a lipopeptide biosurfactant extracted from Corn Steep Liquor has been developed to eliminate polycyclic aromatic hydrocarbons (PAHs) from sewage sludge. High concentrations of PAHs were used during experiments observing that 408.3 mg/kg of naphthalene was almost completely mobilized and biodegraded, only 1.7% of naphthalene remained in the sewage sludge, whereas anthracene and pyrene were reduced up to 51.7 and 69.4%, respectively. The biodegradation of PAHs was fitted to several kinetic models (zero- and first-order kinetic models), observing good correlation coefficient values when biodegradation was described by the first-order kinetic model. Experimental results suggest that biosurfactant extracted from Corn Steep Liquor may have great potential, as an ecofriendly washing agent, for the treatment of sewage sludge contaminated with PAHs. Therefore, in situ application of natural biosurfactants may be considered to be a good remediation alternative...
-
optimization of liquid liquid extraction of biosurfactants from Corn Steep Liquor
Bioprocess and Biosystems Engineering, 2015Co-Authors: X Vecino, J M Cruz, Letricia Barbosapereira, R Devesarey, A B MoldesAbstract:In this work, the optimization of the operational conditions for the chloroform-based extraction of surface-active compounds from Corn Steep Liquor (CSL) was carried out and the nutritional properties of the remnant aqueous phase (CSL-less biosurfactant) was evaluated as microbial fermentation medium. The optimal conditions to obtain biosurfactants from CSL were as follows: chloroform/CSL ratio 2 (v/v), 56 °C at extraction times >30 min. At the optima conditions, 100 % of biosurfactant extract can be obtained from CSL, obtaining 12.0 ± 0.5 g of biosurfactant extract/Kg of CSL. The critical micelle concentration (CMC) of the biosurfactant extract was 399.4 mg L−1. This value is similar to the CMC of cetrimonium bromide (CTAB), a cationic surfactant used in the formulation of nanoparticles. The extraction of biosurfactant can be also carried out at room temperature although in this case, the extraction yield decreased about 15 %. The extraction of surface-active compounds from agroindustrial streams can suppose important advances for the bio-based surfactants industry. Biosurfactants obtained in this work are not only more eco-friendly than chemical detergents but also can be cost competitive with its chemical counterparts. Furthermore, after the extraction of surface-active compounds, CSL-less biosurfactant was found to be suitable as nutritional supplement for lactic acid bacteria, maintaining its nutritional properties in comparison with regular CSL.
Morio Shoda - One of the best experts on this subject based on the ideXlab platform.
-
utilization of the buffering capacity of Corn Steep Liquor in bacterial cellulose production by acetobacter xylinum
Applied Microbiology and Biotechnology, 2004Co-Authors: Naoki Noro, Yasushi Sugano, Morio ShodaAbstract:Acetobacter xylinum BPR2001 produces water-insoluble bacterial cellulose (BC). Using a pH sensor for the accurate control of pH, which is one of the most critical factors for efficient BC production, is difficult especially in a baffled shake-flask and an airlift reactor. The buffering capacity of Corn Steep Liquor (CSL) was estimated by measuring β (buffering capacity) values in advance and was used to maintain the pH within the optimal range during the production of BC. When CSL was added to either a shake-flask, a stirred-tank reactor or an airlift reactor, BC production was almost the same as that in cultivations where pH was controlled manually or by a pH sensor.
-
Utilization of the buffering capacity of Corn Steep Liquor in bacterial cellulose production by Acetobacter xylinum
Applied Microbiology and Biotechnology, 2004Co-Authors: Naoki Noro, Yasushi Sugano, Morio ShodaAbstract:Acetobacter xylinum BPR2001 produces water-insoluble bacterial cellulose (BC). Using a pH sensor for the accurate control of pH, which is one of the most critical factors for efficient BC production, is difficult especially in a baffled shake-flask and an airlift reactor. The buffering capacity of Corn Steep Liquor (CSL) was estimated by measuring beta (buffering capacity) values in advance and was used to maintain the pH within the optimal range during the production of BC. When CSL was added to either a shake-flask, a stirred-tank reactor or an airlift reactor, BC production was almost the same as that in cultivations where pH was controlled manually or by a pH sensor.
Jun Du - One of the best experts on this subject based on the ideXlab platform.
-
classification and analysis of Corn Steep Liquor by uplc q tof ms and hplc
Talanta, 2013Co-Authors: Xue Xiao, Jun Du, Hongzhi Zhao, Linyi Dong, Yiming WangAbstract:Abstract Corn Steep Liquor (CSL), an important raw material with high nutritional value, serves as a nitrogen source in the fermentation industry. The CSL quality directly affects the yield and quality of fermentation products. In this work, a fingerprinting technique was used to identify the potential markers of CSL. Forty-two CSL samples from different manufacturers were profiled by ultra-performance liquid chromatography with tandem quadrupole time-of-flight mass spectrometry. Sixteen compounds, almost all of which were amino acids and their derivatives, were considered as the potential markers. Then, o -phthalaldehyde-9-fluorenylmethyl chloroformate precolumn derivatization by high-performance liquid chromatography was performed to identify the free amino acids in CSL. Principal component analysis (PCA) was used to distinguish among the samples from different manufacturers. The results demonstrated that the fingerprinting technique combined with PCA analysis was a powerful tool for determining the CSL quality.
-
Classification and analysis of Corn Steep Liquor by UPLC/Q-TOF MS and HPLC
Talanta, 2013Co-Authors: Xue Xiao, Jun Du, Hongzhi Zhao, Linyi Dong, Yiming WangAbstract:Abstract Corn Steep Liquor (CSL), an important raw material with high nutritional value, serves as a nitrogen source in the fermentation industry. The CSL quality directly affects the yield and quality of fermentation products. In this work, a fingerprinting technique was used to identify the potential markers of CSL. Forty-two CSL samples from different manufacturers were profiled by ultra-performance liquid chromatography with tandem quadrupole time-of-flight mass spectrometry. Sixteen compounds, almost all of which were amino acids and their derivatives, were considered as the potential markers. Then, o -phthalaldehyde-9-fluorenylmethyl chloroformate precolumn derivatization by high-performance liquid chromatography was performed to identify the free amino acids in CSL. Principal component analysis (PCA) was used to distinguish among the samples from different manufacturers. The results demonstrated that the fingerprinting technique combined with PCA analysis was a powerful tool for determining the CSL quality.
-
determination of vitamins b2 b3 b6 and b7 in Corn Steep Liquor by nir and plsr
Transactions of Tianjin University, 2012Co-Authors: Xue Xiao, Jun DuAbstract:Corn Steep Liquor (CSL) is an important raw material that has high nutritional value and serves as a nitrogen source. Biotin in CSL is especially of great importance to fermentation. In order to develop a fast, versatile, cheap, and environmentally safe analytical method for quantifying vitamins B2 (VB2), B3 (VB3), B6 (VB6) and B7 (VB7) in CSL, the near-infrared spectroscopy (NIR) measurements of 66 samples (22 batches) of CSL were analyzed by partial least-square regression (PLSR). Multivariate models developed in the NIR regions showed good predictive abilities for VB2, VB3, VB6 and VB7. Results confirmed the probability of the multivariate spectroscopic approach as a replacement for expensive and time-consuming conventional chemical methods.
-
Determination of Vitamins B2, B3, B6 and B7 in Corn Steep Liquor by NIR and PLSR *
Transactions of Tianjin University, 2012Co-Authors: Xue Xiao, Jun DuAbstract:Corn Steep Liquor (CSL) is an important raw material that has high nutritional value and serves as a nitrogen source. Biotin in CSL is especially of great importance to fermentation. In order to develop a fast, versatile, cheap, and environmentally safe analytical method for quantifying vitamins B2 (VB2), B3 (VB3), B6 (VB6) and B7 (VB7) in CSL, the near-infrared spectroscopy (NIR) measurements of 66 samples (22 batches) of CSL were analyzed by partial least-square regression (PLSR). Multivariate models developed in the NIR regions showed good predictive abilities for VB2, VB3, VB6 and VB7. Results confirmed the probability of the multivariate spectroscopic approach as a replacement for expensive and time-consuming conventional chemical methods.
X Vecino - One of the best experts on this subject based on the ideXlab platform.
-
ionic behavior assessment of surface active compounds from Corn Steep Liquor by exchange resins
Journal of Surfactants and Detergents, 2017Co-Authors: L Rodriguezlopez, M Rinconfontan, X Vecino, J M Cruz, A B MoldesAbstract:Depending on their ionic nature, biosurfactants can be classified as nonionic, anionic, cationic, or amphoteric. The ionic behavior of biosurfactants is an important characteristic that dictates their use in industrial applications. In this work, a biosurfactant extract obtained from Corn Steep Liquor was subjected to anionic or cationic resins, in order to study the ionic behavior under different operational conditions using response surface methodology. The independent variables included in the study are the dilution of biosurfactant solution, the amount of cationic or anionic resin, and the extraction time, whereas the dependent variables studied consisted of the surface tension of biosurfactant aqueous solution, after contacting with anionic or cationic resin. The results showed that biosurfactant extracted from Corn Steep Liquor is amphoteric, since both resins were able to entrap this biosurfactant, making it particularly suited for use in personal care preparations for sensitive skin.
-
a multifunctional extract from Corn Steep Liquor antioxidant and surfactant activities
Food & Function, 2016Co-Authors: L Rodriguezlopez, X Vecino, A B Moldes, Letricia Barbosapereira, J M CruzAbstract:In the last few years the awareness of consumers and institutions about the impact that our industrial processes has on health and the environment has increased, demanding more natural products. In this work, a multifunctional bioactive extract with surfactant and antioxidant properties, composed mainly of C16 and C18 fatty acids, and phenolic compounds (vanillic acid, p-coumaric acid, ferulic acid, sinapic acid and quercetin) was obtained from Corn Steep Liquor (CSL). Different liquid–liquid extraction protocols were evaluated obtaining a natural extract, which was able to reduce the surface tension of water by more than 30 units, showing antioxidant activity with an EC50 of 8.51 mg mL−1 and a yield of 6.85 g of extract per kg of CSL. Additionally, it was observed that after liquid–liquid extraction with chloroform, the aqueous phase can be subjected to a hydrothermal treatment, followed by extraction with ethyl acetate, in order to obtain another extract (24.7 g of extract per kg of CSL) with a higher antioxidant capacity (EC50 of 4.02 mg mL−1). In this case the antioxidant extract is composed of protocatechuic acid, vanillic acid, caffeic acid, epicatechin, p-coumaric acid, ferulic acid, sinapic acid and quercetin.
-
Optimization of liquid–liquid extraction of biosurfactants from Corn Steep Liquor
Bioprocess and Biosystems Engineering, 2015Co-Authors: X Vecino, J M Cruz, L. Barbosa-pereira, R. Devesa-rey, A B MoldesAbstract:In this work, the optimization of the operational conditions for the chloroform-based extraction of surface-active compounds from Corn Steep Liquor (CSL) was carried out and the nutritional properties of the remnant aqueous phase (CSL-less biosurfactant) was evaluated as microbial fermentation medium. The optimal conditions to obtain biosurfactants from CSL were as follows: chloroform/CSL ratio 2 (v/v), 56 °C at extraction times >30 min. At the optima conditions, 100 % of biosurfactant extract can be obtained from CSL, obtaining 12.0 ± 0.5 g of biosurfactant extract/Kg of CSL. The critical micelle concentration (CMC) of the biosurfactant extract was 399.4 mg L^−1. This value is similar to the CMC of cetrimonium bromide (CTAB), a cationic surfactant used in the formulation of nanoparticles. The extraction of biosurfactant can be also carried out at room temperature although in this case, the extraction yield decreased about 15 %. The extraction of surface-active compounds from agroindustrial streams can suppose important advances for the bio-based surfactants industry. Biosurfactants obtained in this work are not only more eco-friendly than chemical detergents but also can be cost competitive with its chemical counterparts. Furthermore, after the extraction of surface-active compounds, CSL-less biosurfactant was found to be suitable as nutritional supplement for lactic acid bacteria, maintaining its nutritional properties in comparison with regular CSL.
-
sewage sludge polycyclic aromatic hydrocarbon pah decontamination technique based on the utilization of a lipopeptide biosurfactant extracted from Corn Steep Liquor
Journal of Agricultural and Food Chemistry, 2015Co-Authors: X Vecino, L Rodriguezlopez, J M Cruz, A B MoldesAbstract:A decontamination technique based on the utilization of a lipopeptide biosurfactant extracted from Corn Steep Liquor has been developed to eliminate polycyclic aromatic hydrocarbons (PAHs) from sewage sludge. High concentrations of PAHs were used during experiments observing that 408.3 mg/kg of naphthalene was almost completely mobilized and biodegraded, only 1.7% of naphthalene remained in the sewage sludge, whereas anthracene and pyrene were reduced up to 51.7 and 69.4%, respectively. The biodegradation of PAHs was fitted to several kinetic models (zero- and first-order kinetic models), observing good correlation coefficient values when biodegradation was described by the first-order kinetic model. Experimental results suggest that biosurfactant extracted from Corn Steep Liquor may have great potential, as an ecofriendly washing agent, for the treatment of sewage sludge contaminated with PAHs. Therefore, in situ application of natural biosurfactants may be considered to be a good remediation alternative...
-
optimization of liquid liquid extraction of biosurfactants from Corn Steep Liquor
Bioprocess and Biosystems Engineering, 2015Co-Authors: X Vecino, J M Cruz, Letricia Barbosapereira, R Devesarey, A B MoldesAbstract:In this work, the optimization of the operational conditions for the chloroform-based extraction of surface-active compounds from Corn Steep Liquor (CSL) was carried out and the nutritional properties of the remnant aqueous phase (CSL-less biosurfactant) was evaluated as microbial fermentation medium. The optimal conditions to obtain biosurfactants from CSL were as follows: chloroform/CSL ratio 2 (v/v), 56 °C at extraction times >30 min. At the optima conditions, 100 % of biosurfactant extract can be obtained from CSL, obtaining 12.0 ± 0.5 g of biosurfactant extract/Kg of CSL. The critical micelle concentration (CMC) of the biosurfactant extract was 399.4 mg L−1. This value is similar to the CMC of cetrimonium bromide (CTAB), a cationic surfactant used in the formulation of nanoparticles. The extraction of biosurfactant can be also carried out at room temperature although in this case, the extraction yield decreased about 15 %. The extraction of surface-active compounds from agroindustrial streams can suppose important advances for the bio-based surfactants industry. Biosurfactants obtained in this work are not only more eco-friendly than chemical detergents but also can be cost competitive with its chemical counterparts. Furthermore, after the extraction of surface-active compounds, CSL-less biosurfactant was found to be suitable as nutritional supplement for lactic acid bacteria, maintaining its nutritional properties in comparison with regular CSL.