The Experts below are selected from a list of 180393 Experts worldwide ranked by ideXlab platform
Ruichao Lin - One of the best experts on this subject based on the ideXlab platform.
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identification of five gelatins by ultra performance Liquid Chromatography time of flight mass spectrometry uplc q tof ms using principal component analysis
Journal of Pharmaceutical and Biomedical Analysis, 2012Co-Authors: Xianlong Cheng, Feng Wei, Xinyue Xiao, Yingyong Zhao, Yan Shi, Wei Liu, Ping Zhang, Shousheng Tian, Ruichao LinAbstract:An Ultra-Performance Liquid Chromatography-quadrupole-time of flight mass spectrometry (UPLC/Q-TOF-MS) method coupled with a principal component analysis (PCA) was developed and applied toward identifying donkey-hide gelatin, bovine-hide gelatin, pig-hide gelatin, tortoise shell glue, and deerhorn glue. The UPLC-MS data of the trypsin digested samples were subjected to principal component analysis (PCA) in order to classify these five gelatins. Additionally, marker peptides given by the loadings plot of PCA were identified based on a comparison of recorded LC-MS data with a previously reported database of the corresponding gelatin variants. The results from this study indicate that the proposed method is reliable, and it has been successfully applied to the identification of variants of gelatins commonly used in Traditional Chinese Medicine (TCM).
Jiangshan Wang - One of the best experts on this subject based on the ideXlab platform.
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systems toxicology study of doxorubicin on rats using ultra performance Liquid Chromatography coupled with mass spectrometry based metabolomics
Metabolomics, 2009Co-Authors: Shuangqing Peng, Rob Van Der Heijden, Jiangshan Wang, Guowang Xu, Lijuan Chen, Theo H Reijmers, Thomas Hankemeier, Mei Wang, Jan Van Der GreefAbstract:A metabolomics-based systems toxicology approach was used to profile the urinary metabolites for the toxicity related processes and pathogenesis induced by doxorubicin (DOX) to rats. Endogenous metabolite profiles were obtained with ultra performance Liquid Chromatography-mass spectrometry (UPLC-MS) for rats receiving different single dosages of DOX (5, 10 or 20 mg/kg) prior and at three time points after dosage. Principal components analysis (PCA) allowed detection of two major systemic metabolic changes with the time due to the induced toxicity. Furthermore, Analysis of variance (ANOVA) Simultaneous Component Analysis (ASCA) was applied to reveal the variation caused by time and dose, and their interaction in a multivariate way. Finally, various metabolites involved in the toxic processes could be identified using their accurate mass and MSn experiments, and possible mechanisms of the toxicity of DOX were postulated. In conclusion, metabolomics as a systems toxicology approach was able to provide comprehensive information on the dynamic process of drug induced toxicity. In addition, detection of the systemic toxic effects could be obtained with metabolomics at an earlier stage compared to the clinical chemistry and histopathological assessment.
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systems toxicology study of doxorubicin on rats using ultra performance Liquid Chromatography coupled with mass spectrometry based metabolomics
Metabolomics, 2009Co-Authors: Shuangqing Peng, Rob Van Der Heijden, Jiangshan Wang, Guowang Xu, Lijuan Chen, Theo H Reijmers, Thomas Hankemeier, Mei Wang, Jan Van Der GreefAbstract:A metabolomics-based systems toxicology approach was used to profile the urinary metabolites for the toxicity related processes and pathogenesis induced by doxorubicin (DOX) to rats. Endogenous metabolite profiles were obtained with ultra performance Liquid Chromatography-mass spectrometry (UPLC-MS) for rats receiving different single dosages of DOX (5, 10 or 20 mg/kg) prior and at three time points after dosage. Principal components analysis (PCA) allowed detection of two major systemic metabolic changes with the time due to the induced toxicity. Furthermore, Analysis of variance (ANOVA) Simultaneous Component Analysis (ASCA) was applied to reveal the variation caused by time and dose, and their interaction in a multivariate way. Finally, various metabolites involved in the toxic processes could be identified using their accurate mass and MSn experiments, and possible mechanisms of the toxicity of DOX were postulated. In conclusion, metabolomics as a systems toxicology approach was able to provide comprehensive information on the dynamic process of drug induced toxicity. In addition, detection of the systemic toxic effects could be obtained with metabolomics at an earlier stage compared to the clinical chemistry and histopathological assessment.
Hongwei Luan - One of the best experts on this subject based on the ideXlab platform.
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profiling of yew hair roots from various species using ultra performance Liquid Chromatography electrospray ionization mass spectrometry
Rapid Communications in Mass Spectrometry, 2008Co-Authors: Hongwei Luan, Yanyan Zhang, Xingbao Liu, Li Yang, Zhengtao Wang, Ling YangAbstract:An efficient and sensitive profiling approach to complex yew samples was developed using Ultra-Performance Liquid Chromatography/electrospray ionization mass spectrometry (UPLC/ESI-MS). The UPLC-based method displayed short analytical time and improved peak capability, as well as high sensitivity. The appropriate in-source collision-induced dissociation (CID) energy was employed to produce informative characteristic ions which could be used for stereochemical and sub-structural assignment of yew constituents. The method was successfully applied in the rapid screening of yew hair roots from various species, and 53 constituents including 47 taxoids were detected from partially purified root extract. Notably, C-7 hydroxytaxane stereoisomers could be identified based on their different fragment ions under the optimal profiling conditions. It was also observed that hair roots from different Taxus species exhibited nearly identical chemical distribution, indicating they had similar metabolic frameworks. Additionally, Taxus root resources also display benign medicinal perspective because they have relatively simple chemical profiles and possess high yields of valuable taxanes such as paclitaxel, cephalomannine, 10-deacetylpaclitaxel and 7-xylosyltaxanes.
Jan Van Der Greef - One of the best experts on this subject based on the ideXlab platform.
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systems toxicology study of doxorubicin on rats using ultra performance Liquid Chromatography coupled with mass spectrometry based metabolomics
Metabolomics, 2009Co-Authors: Shuangqing Peng, Rob Van Der Heijden, Jiangshan Wang, Guowang Xu, Lijuan Chen, Theo H Reijmers, Thomas Hankemeier, Mei Wang, Jan Van Der GreefAbstract:A metabolomics-based systems toxicology approach was used to profile the urinary metabolites for the toxicity related processes and pathogenesis induced by doxorubicin (DOX) to rats. Endogenous metabolite profiles were obtained with ultra performance Liquid Chromatography-mass spectrometry (UPLC-MS) for rats receiving different single dosages of DOX (5, 10 or 20 mg/kg) prior and at three time points after dosage. Principal components analysis (PCA) allowed detection of two major systemic metabolic changes with the time due to the induced toxicity. Furthermore, Analysis of variance (ANOVA) Simultaneous Component Analysis (ASCA) was applied to reveal the variation caused by time and dose, and their interaction in a multivariate way. Finally, various metabolites involved in the toxic processes could be identified using their accurate mass and MSn experiments, and possible mechanisms of the toxicity of DOX were postulated. In conclusion, metabolomics as a systems toxicology approach was able to provide comprehensive information on the dynamic process of drug induced toxicity. In addition, detection of the systemic toxic effects could be obtained with metabolomics at an earlier stage compared to the clinical chemistry and histopathological assessment.
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systems toxicology study of doxorubicin on rats using ultra performance Liquid Chromatography coupled with mass spectrometry based metabolomics
Metabolomics, 2009Co-Authors: Shuangqing Peng, Rob Van Der Heijden, Jiangshan Wang, Guowang Xu, Lijuan Chen, Theo H Reijmers, Thomas Hankemeier, Mei Wang, Jan Van Der GreefAbstract:A metabolomics-based systems toxicology approach was used to profile the urinary metabolites for the toxicity related processes and pathogenesis induced by doxorubicin (DOX) to rats. Endogenous metabolite profiles were obtained with ultra performance Liquid Chromatography-mass spectrometry (UPLC-MS) for rats receiving different single dosages of DOX (5, 10 or 20 mg/kg) prior and at three time points after dosage. Principal components analysis (PCA) allowed detection of two major systemic metabolic changes with the time due to the induced toxicity. Furthermore, Analysis of variance (ANOVA) Simultaneous Component Analysis (ASCA) was applied to reveal the variation caused by time and dose, and their interaction in a multivariate way. Finally, various metabolites involved in the toxic processes could be identified using their accurate mass and MSn experiments, and possible mechanisms of the toxicity of DOX were postulated. In conclusion, metabolomics as a systems toxicology approach was able to provide comprehensive information on the dynamic process of drug induced toxicity. In addition, detection of the systemic toxic effects could be obtained with metabolomics at an earlier stage compared to the clinical chemistry and histopathological assessment.
Jana Hajslova - One of the best experts on this subject based on the ideXlab platform.
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ultra performance Liquid Chromatography tandem mass spectrometry a novel challenge in multiresidue pesticide analysis in food
Analytica Chimica Acta, 2006Co-Authors: Tomas Kovalczuk, Martin Jech, Jan Poustka, Jana HajslovaAbstract:Potential of Ultra-Performance Liquid Chromatography (UPLC) separation strategy coupled with tandem (in space) mass spectrometric detection (MS/MS) in multiresidue pesticide analysis was critically assessed. Performance parameters such as number of theoretical plates, height of theoretical plate, peak symmetry and peak capacity were measured/calculated on the basis of data generated by analysis of apple extracts containing 17 (semi)polar pesticides representing various classes of active ingredients of widely used crop protective preparations. Ultra-Performance Liquid Chromatography-tandem mass spectrometry (UPLC-MS/MS) procedure provided improved chromatographic parameters resulting in significantly increased sample throughput including lower solvent consumption and lower limits of quantitation (LOQs) for most of target analytes compared to common method employing conventional high-performance Liquid Chromatography (HPLC) separation.
